Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Date: February 2012
Creator: Horn, Patrick J.; Korte, Andrew R.; Neogi, Purnima B.; Love, Ebony; Fuchs, Johannes; Strupat, Kerstin et al.
Description: Article on the spatial mapping of lipids at cellular resolution in embryos of cotton.
Contributing Partner: UNT College of Arts and Sciences
The Chemical Analysis of the Mebane Cottonseed Kernel

The Chemical Analysis of the Mebane Cottonseed Kernel

Date: 1941
Creator: Hanna, Alvis Nelson
Description: The purpose of this work is to make a fairly complete chemical analysis of the Mebane 804-50 cottonseed kernel. A brief history of cotton plant and the economic value of its products are also presented.
Contributing Partner: UNT Libraries
Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin

Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin

Date: December 2003
Creator: Wilkinson, Jeffery Roland
Description: Three overlapping genomic clones covering 29.0 kilobases of cotton DNA were found to encompass a cluster of two presumptive osmotin genes (OSMI and OSMII) and two osmotin pseudogenes (OSMIII and OSMIV). A segment of 16,007 basepairs of genomic DNA was sequenced from the overlapping genomic clones (GenBank Accessions AY303690 and AF304007). The two cotton osmotin genes were found to have open reading frames of 729 basepairs without any introns, and would encode presumptive osmotin preproteins of 242 amino acids. The open reading frames of the genes are identical in sequence to two corresponding cDNA clones (GenBank Accessions AF192271 and AY301283). The two cDNA inserts are almost full-length, since one lacks codons for the four N-terminal amino acids, and the other cDNA insert lacks the coding region for the 34 N-terminal amino acids. The cotton osmotin preproteins can be identified as PR5 proteins from their similarities to the deduced amino acid sequences of other plant osmotin PR5 preproteins. The preproteins would have N-terminal signal sequences of 24 amino acids, and the mature 24 kilodalton isoforms would likely be targeted for extracellular secretion. Prospective promoter elements, including two ethylene response elements, implicated as being positive regulatory elements in the expression of a ...
Contributing Partner: UNT Libraries
Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants.

Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants.

Date: August 2008
Creator: Zhang, Daiyuan
Description: Fatty acid desaturase 2 (FAD2) enzymes are phosphatidylcholine desaturases occurring as integral membrane proteins in the endoplasmic reticulum membrane and convert monounsaturated oleic acid into polyunsaturated linoleic acid. The major objective of this research was to study the expression and function of two cotton FAD2 genes (the FAD2-3 and FAD2-4 genes) and their possible role in plant sensitivity to environmental stress, since plants may increase the polyunsaturated phospholipids in membranes under environmental stress events, such as low temperature and osmotic stress. Two FAD2 cDNA clones corresponding to the two FAD2 genes have been isolated from a cotton cDNA library, indicating both genes are truly expressed in cotton. Model yeast cells transformed with two cotton FAD2 genes were used to study the chilling sensitivity, ethanol tolerance, and growth rate of yeast cells. The expression patterns of the two FAD2 genes were analyzed by reverse transcription polymerase chain reactions (RT-PCR) and Western blot analyses in cotton plants under different treatment conditions. The coding regions of both FAD2 genes were inserted downstream from the CaMV 35S promoter in the pMDC gateway binary vector system. Five different FAD2/pMDC constructs were transformed into the Arabidopsis fad2 knockout mutant background, and multiple potential transgenic Arabidopsis plant ...
Contributing Partner: UNT Libraries
Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.

Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.

Date: May 2002
Creator: Nampaisansuk, Mongkol
Description: A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six exons are identical in nucleotide sequence to the open reading frame of the corresponding cDNA, and would encode a preprotein of 413 amino acids. The preprotein is identified as a FatB thioesterase from its deduced amino acid sequence similarity to those of other FatB thioesterase preproteins. A 5'-flanking region of 914 bp was sequenced, with the potential promoter/enhancer elements including basic helix-loop-helix elements (E box). Alkaline blot hybridization of cotton genomic DNA suggests the presence at least two FatB1 thioesterase genes in cotton. Four plasmid constructs for both constitutive and seed-specific anti-sense RNA suppression and gene-transgene co- suppression of PATE gene expression were successfully generated. Two overlapping cotton genomic clones were found to encompass a Δ-12 fatty acid desaturase (FAD2-3) ...
Contributing Partner: UNT Libraries
National Plant Genome Initiative

National Plant Genome Initiative

Date: December 2001
Creator: National Science and Technology Council (U.S.). Committee on Science. Interagency Working Group on Plant Genomes.
Description: This report is an update on progress of federal plant genome research. The focus in this report is on plants that are economically important to agribusiness.
Contributing Partner: UNT Libraries
Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Date: May 2007
Creator: Spradling, Kimberly Diane
Description: The expression of two cotton osmotin genes was evaluated in terms of the mRNA and protein expression patterns in response to chemical inducers such as ethylene, hydrogen peroxide, and sodium chloride. Reverse transcriptase-polymerase chain reactions (RT-PCR) indicated that osmotin mRNAs are expressed constitutively in root tissues of cotton plants, and that they are rapidly induced in leaf and stem tissues upon ethylene treatment. Real time RT-PCR indicated that osmotin transcript levels were induced 2 to 4 h after treatment with ethephon. The osmotin mRNA levels appear to increase 12 h after treatment, decrease, and then increase again. The osmotin protein expression patterns were analyzed in Western blot analyses using an anti-osmotin antibody preparation. A 24-KDa protein band was detected from cotton plants treated with the inducers. The 24-KDa osmotin proteins were induced 4 h after treatment with ethephon, while down-regulated 96 h after treatment. Multiple osmotin isoforms were observed to be induced in cotton plants upon treatment with ethephon by two-dimensional gel electrophoresis. One goal of this dissertation research was to genetically engineer two cotton osmotin genes to routinely overproduce their antifungal proteins in transgenic Arabidopsis and cotton plants as a natural defense against fungal infections, using co-cultivation with Agrobacterium ...
Contributing Partner: UNT Libraries
Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings.

Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings.

Access: Use of this item is restricted to the UNT Community.
Date: May 2005
Creator: McHugh, John
Description: N-Acylethanolamines (NAEs) are enriched in seed-derived tissues and are believed to be formed from the membrane phospholipid, N-acylphosphatidylethanolamine (NAPE) via the action of phospholipase D (PLD). In an effort to identify a functional NAPE-PLD in cotton seeds and seedlings, we have screened a cotton seedling cDNA (cotyledon mRNA from 48 h dark grown seedlings) library with a 1.2 kb tobacco partial cDNA fragment encoding the middle third of a putative PLDβ/γ (genbank accession, AF195614) isoform. Six plaques were isolated from the Uni-ZAP lambda library, excised as pBluescript SK(-) phagemids and subjected to nucleotide sequence analysis. Alignment of derived sequences with Arabidopsis PLD family members indicated that the cDNAs represent six different PLD gene products -three putative PLD β isoforms and three putative PLD δ isoforms. The PLD β isoforms, designated Ghpldβ1a, GHpldβ1b and a truncated Ghpldβ1b isoform. Both the full-length PLD β proteins contained characteristic HKxxxxD catalytic domains, a PC-binding domain, a PIP2-binding domain and a C2 domain. In addition both cotton PLD β isoforms had a N-terminal "SPQY" rich domain which appeared to be unique to these PLDs. The three PLD δ isoforms, designated Ghpldδ1a, Ghpldδ1b and Ghpldδ1b-2 encode full-length PLDδ proteins, and like the above PLDs, contained the ...
Contributing Partner: UNT Libraries
Interweaving History: The Texas Textile Mill and McKinney, Texas, 1903-1968.

Interweaving History: The Texas Textile Mill and McKinney, Texas, 1903-1968.

Date: August 2009
Creator: Kilgore, Deborah Katheryn
Description: Texas textile mills comprise an untold part of the modern South. The bulk of Texas mills were built between 1890 and 1925, a compressed period of expansion in contrast to the longer developmental pattern of mills in the rest of the United States. This compression meant that Texas mill owners benefited from knowledge gained from mill expansion elsewhere, and owners ran their mills along the same lines as the dominant southeastern model. Owners veered from the established pattern when conditions warranted. This case study focuses on three mills in Texas that operated both independently and as a corporation for a total of sixty years. One mill in McKinney dominated the economy of a small town and serves as the primary focus of this paper. A second mill in Waco served a diversified economy in the center of the state; and the third mill, built in Dallas was concentrated in a major city in a highly competitive job market. All three of these mills will illuminate the single greatest difference between Texas mills and mills elsewhere, the composition of the labor force. Women did not dominate the mill labor force in Texas nor did children, except in limited cases, make-up a ...
Contributing Partner: UNT Libraries
Genetic Modification of Fatty Acid Profiles in Cotton

Genetic Modification of Fatty Acid Profiles in Cotton

Access: Use of this item is restricted to the UNT Community.
Date: August 2005
Creator: Rommel, Amy A.
Description: The industrial uses of cottonseed oil are limited by its fatty acid composition. Genetic modification of cotton lipid profiles using seed-specific promoters could allow cotton growers to produce valuable new oils in the seed without adverse effects on fiber quality and yield, therefore making this crop more commercially profitable. Transgenic cotton callus harboring a diverged fatty acid desaturase gene (FADX) from Momordica charantia was characterized for production of alpha-eleostearic acid (conjugated double bonds: 18:3 D9 cis, 11 trans, 13 trans), not normally found in cotton. Gas chromatography (GC) in conjunction with mass spectrometry (MS) confirmed production of alpha-eleostearic acid in the transgenic cotton tissues. A second series of transformation experiments introduced the cotton fatty acid thioesterase B (FATB) cDNA, fused to the seed-specific oleosin promoter into cotton to promote the over-expression of FATB, to generate cotton with increased palmitate in the cottonseed. PCR amplification, as well as fatty acid analysis by gas chromatography, confirmed introduction of the FATB cDNA in transgenic tissues. Collectively, these results demonstrate the feasibility of manipulating the fatty acid composition in cotton via transgenic approaches and form the basis for continued efforts to create novel oils in cottonseed.
Contributing Partner: UNT Libraries
Method of Enhancing Quality Factors in Cotton

Method of Enhancing Quality Factors in Cotton

Date: June 9, 2006
Creator: Hake, Kater Davis; Chapman, Kent D.; Kerby, Thomas Arthur & Speed, Thomas Rainey
Description: Patent relating to a method of enhancing quality factors in cotton.
Contributing Partner: UNT College of Arts and Sciences
Expression of genes associated with carbohydrate metabolism in cotton stems and roots

Expression of genes associated with carbohydrate metabolism in cotton stems and roots

Date: January 22, 2009
Creator: Taliercio, Earl W.; Romano, Gabriela; Scheffler, Jodi & Ayre, Brian G.
Description: Article discussing the expression of genes associated with carbohydrate metabolism in cotton stems and roots.
Contributing Partner: UNT College of Arts and Sciences
A Study of the Effect of Three "Non-Rinsing" Compounds on the Tensile Strength of Cotton Percale

A Study of the Effect of Three "Non-Rinsing" Compounds on the Tensile Strength of Cotton Percale

Date: 1951
Creator: Bell, Mildred L.
Description: The purpose of the present study is to determine the effect of three "non-rinse" washing compounds upon the tensile strength of cotton percale, in order to have some basis for recommendation as to use when teaching laundering to homemaking students and homemakers.
Contributing Partner: UNT Libraries
The Development of the Sugar, Rubber, and Cotton Industries in Brazil

The Development of the Sugar, Rubber, and Cotton Industries in Brazil

Date: 1950
Creator: Miller, James C.
Description: In this study of the development of the sugar, rubber, and cotton industries in Brazil, the writer proposes to show the development of these industries from the beginning of the industries to about 1947.
Contributing Partner: UNT Libraries
Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.)

Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.)

Date: December 1998
Creator: Local, Andrea
Description: Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of roles in animals, plants and bacteria. Cotton genomic and cDNA libraries were screened for the plastidial isoform of carbonic anhydrase. The nucleotide sequences of two 1.2 Kb partial cDNA clones were determined. These clones exhibit high homology to carbonic anhydrases from other dicot plants and possess all the expected peptide motifs. For example, serine and threonine rich chloroplastic targeting peptide and conserved zinc binding residues are both present. These clones were utilized to isolate two carbonic anhydrase genes that were shown to encode different isoforms by PCR and RFLP analysis.
Contributing Partner: UNT Libraries
Cotton seed and its products.

Cotton seed and its products.

Date: June 1896
Creator: United States. Dept. of Agriculture.
Description: Discusses cottonseed and its uses. Describes methods of manufacturing cottonseed products.
Contributing Partner: UNT Libraries Government Documents Department
Farm Practice in the Use of Commercial Fertilizers in the South Atlantic States

Farm Practice in the Use of Commercial Fertilizers in the South Atlantic States

Date: 1910
Creator: Beavers, J. C.
Description: Report discussing the use of fertilizers on the more important soils of the South Atlantic States in the growing of staple farm crops. Factors which influence the use of commercial fertilizers such as crop rotation, legumes, and manure are discussed. In addition, methods for fertilizing cotton, corn, oats, wheat, and cowpea are discussed.
Contributing Partner: UNT Libraries Government Documents Department
A Profitable Cotton Farm

A Profitable Cotton Farm

Date: 1909
Creator: Goodrich, C. L. (Charles Landon)
Description: "This bulletin is an account of the progressive and successful farm operations of a farmer of South Carolina who, by combining thorough tillage, crop rotation, barnyard manure, and a judicious use of commercial fertilizer, has changed a previously badly managed and run-down cotton farm into a very productive and profitable enterprise. The impulse prompting the writing of this bulletin is the belief that it may suggest to other farmers of the South ways and means by which they may so improve their methods of management as to make their farms more profitable." -- p. 7
Contributing Partner: UNT Libraries Government Documents Department
Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.

Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.

Access: Use of this item is restricted to the UNT Community.
Date: May 2002
Creator: Yoder, David W.
Description: Putative cotton cDNA clones and cognate genomic clones for a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE) and an osmotin-like pathogenesis-related 5 (PR5) protein have been isolated and characterized. PATE is a class B fatty acid thioesterase with specificity for saturated long-chain fatty acids such as palmitate, and is implicated as a key enzyme to be targeted for regulation of fatty acid synthesis in order to alter cotton seed oil profiles. A nearly full-length 1.7-kb cDNA clone was isolated using a hybridization probe derived from an Arabidopsis PATE cDNA clone designated TE 3-2. A 17-kb genomic segment encompassing the PATE gene was also isolated, which has six exons and five introns with high sequence identity with other FatB cDNA/gene sequences. The deduced PATE preprotein amino acid sequence of 413 residues has putative signal sequences for targeting to the chloroplast stroma. PR5 proteins called osmotins are made in response to fungal pathogen stress or osmotic stress (water deprivation or salt exposure). Osmotins may actually form pores in fungal membranes, leading to osmotic rupture and destruction of the fungal cells. A cotton osmotin-like PR5 cDNA insert of 1,052 base-pairs was isolated and shown to encode a preprotein of 242 amino acids and is ...
Contributing Partner: UNT Libraries
Child's self-help overall.

Child's self-help overall.

Date: December 1948
Creator: Scott, Clarice L. (Clarice Louisba), b. 1899.
Description: This document contains recommendations for the construction of a child's self-help overall, designed for freedom of movement and easy dressing.
Contributing Partner: UNT Libraries Government Documents Department
Poisoning the cotton boll weevil.

Poisoning the cotton boll weevil.

Date: April 1929
Creator: Coad, B. R. & Gaines, R. C. (Robert Callaway), 1898-
Description: A guide to selecting the most suitable method for controlling the boll weevil. Describes poisons and equipment used in boll-weevil control.
Contributing Partner: UNT Libraries Government Documents Department
Methods and equipment for home laundering.

Methods and equipment for home laundering.

Date: February 1940
Creator: Holbrook, Helen S. (Helen Shepard), b. 1882. & O'Brien, Ruth, b. 1892.
Description: A guide to planning a laundry center at home. Describes various types of equipment and machinery available for washing, drying, and ironing. Describes the products used for laundering and the recommended laundering methods for specific fabric types.
Contributing Partner: UNT Libraries Government Documents Department
Winter legumes for green manure in the Cotton Belt.

Winter legumes for green manure in the Cotton Belt.

Date: May 1942
Creator: McKee, Roland. & McNair, A. D.
Description: Describes varieties of the winter legumes used for "green manure" in the southern United States; provides suggestions for considerations to make when using winter legumes on farmland.
Contributing Partner: UNT Libraries Government Documents Department
The Southern Unity Movement

The Southern Unity Movement

Date: 1956
Creator: Chappell, Ben A.
Description: This thesis describes the history of the Southern unity movement beginning in the mid nineteenth century, with a focus on the legal and political conflicts that surrounded it.
Contributing Partner: UNT Libraries
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