Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Date: February 2012
Creator: Horn, Patrick J.; Korte, Andrew R.; Neogi, Purnima B.; Love, Ebony; Fuchs, Johannes; Strupat, Kerstin et al.
Description: Article on the spatial mapping of lipids at cellular resolution in embryos of cotton.
Contributing Partner: UNT College of Arts and Sciences
Poisoning the cotton boll weevil.

Poisoning the cotton boll weevil.

Date: April 1929
Creator: Coad, B. R. & Gaines, R. C. (Robert Callaway), 1898-
Description: A guide to selecting the most suitable method for controlling the boll weevil. Describes poisons and equipment used in boll-weevil control.
Contributing Partner: UNT Libraries Government Documents Department
The Chemical Analysis of the Mebane Cottonseed Kernel

The Chemical Analysis of the Mebane Cottonseed Kernel

Date: 1941
Creator: Hanna, Alvis Nelson
Description: The purpose of this work is to make a fairly complete chemical analysis of the Mebane 804-50 cottonseed kernel. A brief history of cotton plant and the economic value of its products are also presented.
Contributing Partner: UNT Libraries
Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin

Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin

Date: December 2003
Creator: Wilkinson, Jeffery Roland
Description: Three overlapping genomic clones covering 29.0 kilobases of cotton DNA were found to encompass a cluster of two presumptive osmotin genes (OSMI and OSMII) and two osmotin pseudogenes (OSMIII and OSMIV). A segment of 16,007 basepairs of genomic DNA was sequenced from the overlapping genomic clones (GenBank Accessions AY303690 and AF304007). The two cotton osmotin genes were found to have open reading frames of 729 basepairs without any introns, and would encode presumptive osmotin preproteins of 242 amino acids. The open reading frames of the genes are identical in sequence to two corresponding cDNA clones (GenBank Accessions AF192271 and AY301283). The two cDNA inserts are almost full-length, since one lacks codons for the four N-terminal amino acids, and the other cDNA insert lacks the coding region for the 34 N-terminal amino acids. The cotton osmotin preproteins can be identified as PR5 proteins from their similarities to the deduced amino acid sequences of other plant osmotin PR5 preproteins. The preproteins would have N-terminal signal sequences of 24 amino acids, and the mature 24 kilodalton isoforms would likely be targeted for extracellular secretion. Prospective promoter elements, including two ethylene response elements, implicated as being positive regulatory elements in the expression of a ...
Contributing Partner: UNT Libraries
Cotton seed and its products.

Cotton seed and its products.

Date: June 1896
Creator: United States. Dept. of Agriculture.
Description: Discusses cottonseed and its uses. Describes methods of manufacturing cottonseed products.
Contributing Partner: UNT Libraries Government Documents Department
Winter legumes for green manure in the Cotton Belt.

Winter legumes for green manure in the Cotton Belt.

Date: May 1942
Creator: McKee, Roland. & McNair, A. D.
Description: Describes varieties of the winter legumes used for "green manure" in the southern United States; provides suggestions for considerations to make when using winter legumes on farmland.
Contributing Partner: UNT Libraries Government Documents Department
Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants.

Expression analysis of the fatty acid desaturase 2-4 and 2-3 genes from Gossypium hirsutum in transformed yeast cells and transgenic Arabidopsis plants.

Date: August 2008
Creator: Zhang, Daiyuan
Description: Fatty acid desaturase 2 (FAD2) enzymes are phosphatidylcholine desaturases occurring as integral membrane proteins in the endoplasmic reticulum membrane and convert monounsaturated oleic acid into polyunsaturated linoleic acid. The major objective of this research was to study the expression and function of two cotton FAD2 genes (the FAD2-3 and FAD2-4 genes) and their possible role in plant sensitivity to environmental stress, since plants may increase the polyunsaturated phospholipids in membranes under environmental stress events, such as low temperature and osmotic stress. Two FAD2 cDNA clones corresponding to the two FAD2 genes have been isolated from a cotton cDNA library, indicating both genes are truly expressed in cotton. Model yeast cells transformed with two cotton FAD2 genes were used to study the chilling sensitivity, ethanol tolerance, and growth rate of yeast cells. The expression patterns of the two FAD2 genes were analyzed by reverse transcription polymerase chain reactions (RT-PCR) and Western blot analyses in cotton plants under different treatment conditions. The coding regions of both FAD2 genes were inserted downstream from the CaMV 35S promoter in the pMDC gateway binary vector system. Five different FAD2/pMDC constructs were transformed into the Arabidopsis fad2 knockout mutant background, and multiple potential transgenic Arabidopsis plant ...
Contributing Partner: UNT Libraries
Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.

Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.

Date: May 2002
Creator: Nampaisansuk, Mongkol
Description: A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six exons are identical in nucleotide sequence to the open reading frame of the corresponding cDNA, and would encode a preprotein of 413 amino acids. The preprotein is identified as a FatB thioesterase from its deduced amino acid sequence similarity to those of other FatB thioesterase preproteins. A 5'-flanking region of 914 bp was sequenced, with the potential promoter/enhancer elements including basic helix-loop-helix elements (E box). Alkaline blot hybridization of cotton genomic DNA suggests the presence at least two FatB1 thioesterase genes in cotton. Four plasmid constructs for both constitutive and seed-specific anti-sense RNA suppression and gene-transgene co- suppression of PATE gene expression were successfully generated. Two overlapping cotton genomic clones were found to encompass a Δ-12 fatty acid desaturase (FAD2-3) ...
Contributing Partner: UNT Libraries
Farm Practice in the Use of Commercial Fertilizers in the South Atlantic States

Farm Practice in the Use of Commercial Fertilizers in the South Atlantic States

Date: 1910
Creator: Beavers, J. C.
Description: Report discussing the use of fertilizers on the more important soils of the South Atlantic States in the growing of staple farm crops. Factors which influence the use of commercial fertilizers such as crop rotation, legumes, and manure are discussed. In addition, methods for fertilizing cotton, corn, oats, wheat, and cowpea are discussed.
Contributing Partner: UNT Libraries Government Documents Department
A Profitable Cotton Farm

A Profitable Cotton Farm

Date: 1909
Creator: Goodrich, C. L. (Charles Landon)
Description: "This bulletin is an account of the progressive and successful farm operations of a farmer of South Carolina who, by combining thorough tillage, crop rotation, barnyard manure, and a judicious use of commercial fertilizer, has changed a previously badly managed and run-down cotton farm into a very productive and profitable enterprise. The impulse prompting the writing of this bulletin is the belief that it may suggest to other farmers of the South ways and means by which they may so improve their methods of management as to make their farms more profitable." -- p. 7
Contributing Partner: UNT Libraries Government Documents Department
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