Investigation of the Pharmacokinetics of Diazepam in Juvenile Channel Catfish (Ictalurus Punctatus)

Investigation of the Pharmacokinetics of Diazepam in Juvenile Channel Catfish (Ictalurus Punctatus)

Date: December 2013
Creator: Overturf, Carmen L.
Description: The presence of pharmaceuticals in the environment is becoming an increasing regulatory and scientific concern. Thus, the metabolic profile and bioconcentration potential of diazepam, a model benzodiazepine, were examined, as well as effects on the endocrine system in channel catfish. Through the use of specific and non-specific cytochrome P450 (CYP450) inhibitors, it was determined that CYP3A-like enzymes may play a role in the biotransformation of diazepam into temazepam; however, the isoform(s) required for the formation of other metabolites is still unknown. Overall, only around 7-8% of diazepam is biotransformed into two known metabolites. Due to the lack of inherent metabolism of diazepam in channel catfish, further analysis was conducted to determine the tissue-specific bioconcentration potential of diazepam in catfish. Various tissues were analyzed for the presence of diazepam as well as metabolites and bioconcentration factors (BCF) were calculated, which were all well below regulatory threshold values (> 2000). Additionally, modulation of the endocrine system by diazepam was examined by measuring steroid hormone concentrations and analyzing mRNA expression of selected steroidogenic enzymes and receptors. Two steroidogenic enzymes were modulated following diazepam exposure, indicating potential endocrine disrupting properties of diazepam. Together, these data suggest that diazepam exhibits low metabolic transformation rates in ...
Contributing Partner: UNT Libraries
Solubility of Benzodiazepines in Polyethylene Glycol 200 + Water Mixtures at 303.2 K

Solubility of Benzodiazepines in Polyethylene Glycol 200 + Water Mixtures at 303.2 K

Date: June 15, 2009
Creator: Jouyban, Abolghasem; Shokri, Javad; Barzegar-Jalali, Mohammad; Hassanzadeh, Davoud; Acree, William E. (William Eugene); Ghafourian, Taravat et al.
Description: Article discussing the solubility of benzodiazepines in polyethylene glycol 200 plus water mixtures at 303.2 K.
Contributing Partner: UNT College of Arts and Sciences
Solubility of Chlordiazepoxide, Diazepam, and Lorazepam in Ethanol + Water Mixtures at 303.2 K

Solubility of Chlordiazepoxide, Diazepam, and Lorazepam in Ethanol + Water Mixtures at 303.2 K

Date: April 22, 2009
Creator: Jouyban, Abolghasem; Shokri, Javad; Barzegar-Jalali, Mohammad; Hassanzadeh, Davoud; Acree, William E. (William Eugene); Ghafourian, Taravat et al.
Description: Article discussing the solubility of chlordiazepoxide, diazepam, and lorazepam in ethanol + water mixtures at 303.2 K.
Contributing Partner: UNT College of Arts and Sciences
Benzodiazepines and their Effects on Cognitive-Behavioral Therapy

Benzodiazepines and their Effects on Cognitive-Behavioral Therapy

Date: April 14, 2011
Creator: Reichenberger, David A. & Taylor, Daniel J.
Description: Poster presentation for the 2011 University Scholars Day at the University of North Texas discussing research on benzodiazepines and their effects on cognitive-behavioral therapy.
Contributing Partner: UNT Honors College