Description: In this book, the authors discuss WebGBrowse 2.1. Genome browsers are critical bioinformatics tools for biologists to visualize genome annotations and the other sequence features along a reference sequence. GBrowse is one of the most popular genome browsers used by the research community. However, its installation and configuration prove to be difficult for many biologists. The authors have developed a web server, WebGBrowse, which takes a user-supplied annotation file in GFF3 format, guides users through the configuration of the display of each genomic feature, and allows them to visualize the genome annotation information via the GBrowse software. This book describes an upgraded WebGBrowse server, WebGBrowse 2.0, which provides users with a choice to display their genome annotation with different versions of the GBrowse software.