Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle

Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle

Date: December 2010
Creator: Akel, Amal
Description: Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single quantum dots were photoactivated, localized and reduced to their centroid and then reconstructed to a super-resolution image. The acquired super-resolution image shows a lateral and an axial sub-diffraction resolution and demonstrates ultrafine striations with widths less than 70 nm that are not evident by conventional confocal microscopy. The striations appear to be related to nebulin thin filament binding protein. This newly developed imaging system is cutting edge for its high resolution and localization as well its simplicity and convenience.
Contributing Partner: UNT Libraries
De wonderlijke openbarigen van de microscoop

De wonderlijke openbarigen van de microscoop

Date: 1965 - 1973
Creator: Franco-Suisse
Description: An overview of the use of microscopes, and the types of things that can be seen through them.
Contributing Partner: UNT Libraries Rare Book and Texana Collections
Materials Evaluation and Inspection : New Methods, Materials and Dimensions, Proceedings of the Conference Held at Argonne National Laboratory, September 25-26, 1989

Materials Evaluation and Inspection : New Methods, Materials and Dimensions, Proceedings of the Conference Held at Argonne National Laboratory, September 25-26, 1989

Date: November 1990
Creator: Nevitt, Michael V. & Peterson, Norman D.
Description: A conference at Argonne National Laboratory for executives and technical personnel in small and medium-size manufacturing companies summarized how materials evaluation and inspection by nondestructive methods are changing and broadening. The application of these rapidly emerging techniques in maintaining and improving the quality and competitiveness of manufactured products was stressed. Individual papers have been cataloged separately.
Contributing Partner: UNT Libraries Government Documents Department
Analytical Electron Microscopy Characterization of Uranium-Contaminated Soils from the Fernald Site, FY1993 Report

Analytical Electron Microscopy Characterization of Uranium-Contaminated Soils from the Fernald Site, FY1993 Report

Date: October 1994
Creator: Buck, E. C.; Cunnane, J. C.; Brown, N. R. & Dietz, N. L.
Description: A combination of optical microscopy, scanning electron microscopy with backscattered electron detection (SEM/BSE), and analytical electron microscopy (AEM) is being used to determine the nature of uranium in soils from the Fernald Environmental Management Project. The information gained from these studies is being used to develop and test remediation technologies. Investigations using SEM have shown that uranium is contained within particles that are typically 1 to 100 micrometers in diameter. Further analysis with AEM has shown that these uranium-rich regions are made up of discrete uranium-bearing phases. The distribution of these uranium phases was found to be inhomogeneous at the microscopic level.
Contributing Partner: UNT Libraries Government Documents Department
Standard Test Specimens of Zinc Bronze (Cu 88, Sn 10, Zn 2) - Parts 1 and 2: Part 1. - Preparation of Specifications, Part II. - Microstructure

Standard Test Specimens of Zinc Bronze (Cu 88, Sn 10, Zn 2) - Parts 1 and 2: Part 1. - Preparation of Specifications, Part II. - Microstructure

Date: March 15, 1916
Creator: Karr, C. P. & Rawdon, Henry S.
Description: Technical paper issued by the Bureau of Standards over tests conducted on the preparation of an alloy. The tests conducted, and their results are presented and discussed. This paper includes tables, illustrations, and photographs.
Contributing Partner: UNT Libraries Government Documents Department
Analytical Electron Microscopy Characterization of Fernald soils. Annual Report, October 1993 - September 1994

Analytical Electron Microscopy Characterization of Fernald soils. Annual Report, October 1993 - September 1994

Date: March 1995
Creator: Buck, E. C.; Brown, N. R. & Dietz, N. L.
Description: A combination of backscattered electron imaging and analytical electron microscopy (AEM) with electron diffraction have been used to determine the physical and chemical properties of uranium contamination in soils from the Fernald Environmental Management Project in Ohio. The information gained from these studies has been used in the development and testing of remediation technologies.
Contributing Partner: UNT Libraries Government Documents Department