3 Matching Results

Search Results

Results: 1 - 3
open access

Applications of Nanomanipulation Coupled to Nanospray Mass Spectrometry in Trace Fiber Analysis and Cellular Lipid Analysis.

Description: The novel instrumentation of nanomanipulation coupled to nanospray mass spectrometry and its applications are presented. The nanomanipulator has the resolution of 10nm step sizes allowing for specific fine movement used to probe and characterize objects of interest. Nanospray mass spectrometry only needs a minimum sample volume of 300nl and a minimum sample size of 300attograms to analyze an analyte making it the ideal instrument to couple to nanomanipulation. The nanomanipulator is mounted to … more
Date: December 2008
Creator: Ledbetter, Nicole
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Lipidomic Analysis of Single Cells and Organelles Using Nanomanipulation Coupled to Mass Spectrometry

Description: The capability to characterize disease states by way of determining novel biomarkers has led to a high demand of single cell and organelle analytical methodologies due to the unexpected heterogeneity present in cells of the same type. Lipids are of particular interest in the search for biomarkers due to their active roles in cellular metabolism and energy storage. Analyzing localized lipid chemistry from individual cells and organelles is challenging however, due to low analyte volume, limited … more
Date: May 2016
Creator: Bowman, Amanda
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

A Rapid, Accurate Thin Layer Chromatographic Analysis of Phospholipids and Neutral Lipids

Description: A modified ascending thin layer chromatographic technique has been developed which resolves the major phospholipid and neutral lipid classes of five common fluids and tissues. A one-half milliliter sample is extracted with n-butanol:diisopropylether (40:60 by volume, cholesteryl acetate = 100 ng/ul) for thirty minutes and the organic phase is spotted onto a thin layer chromatography plate and carried through three successive chromatographic developments. The lipids are then visualized either by… more
Date: December 1979
Creator: Wilson, Charlie W.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
Back to Top of Screen