Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle
Description:
Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single q…
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Date:
December 2010
Creator:
Akel, Amal
Partner:
UNT Libraries