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open access

FLP-mediated conditional loss of an essential gene to facilitate complementation assays

Description: Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious and time-consuming step, the newly developed two-component system utilizes a site-specific recombinase to excise a wild-type copy of the gene of interest from transformed tissues. In the first componen… more
Date: December 2007
Creator: Ganesan, Savita
Partner: UNT Libraries
open access

Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

Description: This article discusses functional characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter.
Date: September 2008
Creator: Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. & Ayre, Brian G.
Partner: UNT College of Arts and Sciences
open access

Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Description: This article discusses the effect and overall importance of the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene on carbon partitioning and plant growth.
Date: January 20, 2009
Creator: Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. & Ayre, Brian G.
Partner: UNT College of Arts and Sciences
open access

A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

Description: Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.
Date: April 15, 2011
Creator: Dasgupta, Kasturi; Ganesan, Savita; Manivasagam, Sindhu & Ayre, Brian G.
Partner: UNT College of Arts and Sciences
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