Identification of functional information subgraphs in cultured neural networks

Identification of functional information subgraphs in cultured neural networks

Date: July 13, 2009
Creator: Gintautas, Vadas; Bettencourt, Luis & Ham, Michael I.
Description: This paper accompanies an oral presentation on the identification of functional information subgraphs in cultured neural networks.
Contributing Partner: UNT College of Arts and Sciences
Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro

Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro

Date: December 2004
Creator: Miller, Benjamin R.
Description: Spontaneously active frontal cortex and spinal cord networks grown on microelectrode arrays were used to study effects of D-methylphenidate. These central nervous system tissues have relatively low concentrations of dopaminergic and noradrenergic neurons compared to the richly populated loci, yet exhibit similar neurophysiological responses to methylphenidate. The spontaneous spike activity of both tissues was inhibited in a concentration-dependent manner by serial additions of 1-500 µM methylphenidate. Methylphenidate is non-toxic as spike inhibition was recovered following washes. The average concentrations for 50% spike rate inhibition (IC50 ± SD) were 118 ± 52 (n= 6) and 57 ± 43 (n = 11) for frontal cortex and spinal cord networks, respectively. A 3 hour exposure of a network to 1 mM methylphenidate was nontoxic. The effective concentrations described in this study are within the therapeutic dosage range. Therefore, the platform may be used for further investigations of drug mechanisms.
Contributing Partner: UNT Libraries
Commentary: Feedback stabilizes propagation of synchronous spiking in cortical neural networks

Commentary: Feedback stabilizes propagation of synchronous spiking in cortical neural networks

Date: June 10, 2015
Creator: Claverol-Tinturé, Enric & Gross, Guenter W.
Description: This article provides a commentary on the article "Feedback stabilizes propagation of synchronous spiking in cortical neural networks" by Moldakarimov, S., Bazhenov, M., and Sejnowski, T.J.
Contributing Partner: UNT College of Arts and Sciences
Problems Associated with the Estimation of Nerve Cell Densities in Cultured Neuronal Networks

Problems Associated with the Estimation of Nerve Cell Densities in Cultured Neuronal Networks

Date: Spring 2007
Creator: Pankonien, J. Blake
Description: Thesis written by a student in the UNT Honors College discussing the problems and variables associated with assessing the total number of neurons in a network from cell seeding concentrations and measurements of network area.
Contributing Partner: UNT Honors College
An in vitro model for testing drugs to treat tinnitus

An in vitro model for testing drugs to treat tinnitus

Date: October 2011
Creator: Wu, Calvin; Gopal, Kamakshi V.; Gross, Guenter W.; Lukas, Thomas J. & Moore, Ernest J.
Description: Article discussing an in vitro model for testing drugs to treat tinnitus.
Contributing Partner: UNT College of Arts and Sciences
Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

Date: December 2007
Creator: Santa Maria, Cara L.
Description: This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are discussed. This volume offers value and utility to the rapidly expanding fields of MEA recording and neuronal cell culture. Due to increasing interest in determining the mechanisms underlying Parkinson's disease, the newly developed procedures for mesencephalon isolation and culture on MEAs are an important research contribution.
Contributing Partner: UNT Libraries
Determining Properties of Synaptic Structure in a Neural Network through Spike Train Analysis

Determining Properties of Synaptic Structure in a Neural Network through Spike Train Analysis

Date: May 2007
Creator: Brooks, Evan
Description: A "complex" system typically has a relatively large number of dynamically interacting components and tends to exhibit emergent behavior that cannot be explained by analyzing each component separately. A biological neural network is one example of such a system. A multi-agent model of such a network is developed to study the relationships between a network's structure and its spike train output. Using this model, inferences are made about the synaptic structure of networks through cluster analysis of spike train summary statistics A complexity measure for the network structure is also presented which has a one-to-one correspondence with the standard time series complexity measure sample entropy.
Contributing Partner: UNT Libraries
Pharmacodynamics of potassium channel openers in cultured neuronal networks

Pharmacodynamics of potassium channel openers in cultured neuronal networks

Date: June 5, 2014
Creator: Wu, Calvin; Gopal, Kamakshi V.; Lukas, Thomas J.; Gross, Guenter W. & Moore, Ernest J.
Description: Article discussing pharmacodynamics of potassium channel openers in cultured neuronal networks.
Contributing Partner: UNT College of Arts and Sciences
Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition

Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition

Date: February 15, 2014
Creator: Wu, Calvin; Gopal, Kamakshi V.; Moore, Ernest J. & Gross, Guenter W.
Description: Article on antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition.
Contributing Partner: UNT College of Arts and Sciences
Towards the timely detection of toxicants

Towards the timely detection of toxicants

Date: April 2004
Creator: Ignaccolo, Massimiliano; Grigolini, Paolo & Gross, Guenter W.
Description: Article discussing the problem of enhancing the sensitivity of biosensors to the influence of toxicants, with an entropy method of analysis, denoted as CASSANDRA, for the specific purpose of studying non-stationary time series.
Contributing Partner: UNT College of Arts and Sciences
Assessment of Styrene Oxide Neurotoxicity Using In Vitro Auditory Cortex Networks

Assessment of Styrene Oxide Neurotoxicity Using In Vitro Auditory Cortex Networks

Date: May 24, 2011
Creator: Gopal, Kamakshi V.; Wu, Calvin; Moore, Ernest J. & Gross, Guenter W.
Description: Article on the assessment of styrene oxide neurotoxicity using in vitro auditory cortex networks.
Contributing Partner: UNT College of Arts and Sciences
Improved Fabrication and Quality Control of Substrate Integrated Microelectrode Arrays

Improved Fabrication and Quality Control of Substrate Integrated Microelectrode Arrays

Date: May 2000
Creator: Zim, Bret E.
Description: Spontaneously active monolayer neuronal networks cultured on photoetched multimicroelectrode plates (MMEPs) offer great potential for use in studying neuronal networks. However, there are many problems associated with frequent, long-term use of MMEPs. The major problems include (1) polysiloxane insulation deterioration and breakdown, (2) and loss of gold at the gold electroplated indium-tin oxide (ITO) electrodes. The objective of this investigation was to correct these major problems. Quality control measures were employed to monitor MMEP fabrication variables. The phenotypes of polysiloxane degradation were identified and classified. Factors that were found to contribute most to insulation deterioration were (1) moisture contamination during MMEP insulation, (2) loss of the quartz barrier layer from excessive exposure to basic solutions, and (3) repetitive use in culture. As a result, the insulation equipment and methods were modified to control moisture-dependent insulation deterioration, and the KOH reprocessing solution was replaced with tetramethylguanidine to prevent damage to the quartz. The problems associated with gold electroplating were solved via the addition of a pulsed-DC application of gold in a new citrate buffered electroplating solution.
Contributing Partner: UNT Libraries
Spontaneous coordinated activity in cultured networks: analysis of multiple ignition sites, primary circuits, and burst phase delay distributions

Spontaneous coordinated activity in cultured networks: analysis of multiple ignition sites, primary circuits, and burst phase delay distributions

Date: June 2008
Creator: Ham, Michael I.; Bettencourt, Luis; McDaniel, Floyd Del. (Floyd Delbert), 1942- & Gross, Guenter W.
Description: This article discusses an analysis of multiple ignition sites, primary circuits, and burst phase delay distributions.
Contributing Partner: UNT College of Arts and Sciences
Botulinum Toxin Suppression of CNS Network Activity In Vitro

Botulinum Toxin Suppression of CNS Network Activity In Vitro

Date: July 20, 2013
Creator: Pancrazio, Joseph J.; Gopal, Kamakshi V.; Keefer, Edward W. & Gross, Guenter W.
Description: Article on botulinum toxin suppression of CNS network activity in vitro.
Contributing Partner: UNT College of Arts and Sciences
ReSource, Volume 12, 2000

ReSource, Volume 12, 2000

Date: 2000
Creator: University of North Texas
Description: ReSource magazine includes articles and notes about research at University of North Texas in various academic fields.
Contributing Partner: University Relations, Communications & Marketing department for UNT
Investigation of cryopreservation methods for adherent nerve cell networks in vitro.

Investigation of cryopreservation methods for adherent nerve cell networks in vitro.

Date: December 2009
Creator: Webb, Veronica Fine
Description: Cryopreservation in suspension is commonplace for a variety of cell types. However, cryopreservation of adherent cells has achieved limited success. This research aimed to cryopreserve adherent nerve cell networks in vitro in a manner that preserved network morphology and physiology. Successful implementation would enable long term storage of adherent neuronal networks on microelectrode arrays and on-demand access for use in pharmacological and toxicological testing. Based upon morphological assessments, excellent post-thaw preservation was obtained and post-thaw cultures survived in a transitional medium for up to 3.5 hours. However, transitions to native culture medium post-thaw presented difficulties, ultimately resulting in necrosis. A discussion of methods to supplement the current research and increase post-thaw viability is included in the thesis.
Contributing Partner: UNT Libraries
ReSource, Volume 11, Number 1, Fall 1994

ReSource, Volume 11, Number 1, Fall 1994

Date: 1994
Creator: University of North Texas
Description: ReSource magazine includes articles and notes about research at University of North Texas in various academic fields.
Contributing Partner: University Relations, Communications & Marketing department for UNT
D-Methionine protects against cisplatin-induced neurotoxicity in cortical networks

D-Methionine protects against cisplatin-induced neurotoxicity in cortical networks

Date: June 23, 2012
Creator: Gopal, Kamakshi V.; Wu, Calvin; Shrestha, Bibesh; Campbell, Kathleen C. M.; Moore, Ernest J. & Gross, Guenter W.
Description: Article discussing D-Methionine protecting against cisplatin-induced neurotoxicity in cortical networks.
Contributing Partner: UNT College of Arts and Sciences
The North Texan, Volume 41, Number 2, June 1991

The North Texan, Volume 41, Number 2, June 1991

Date: June 1991
Creator: University of North Texas
Description: The North Texan includes articles and notes about University of North Texas students, faculty, and alumni activities.
Contributing Partner: University Relations, Communications & Marketing department for UNT
ReSource, Volume 4, Number 1, [1987]

ReSource, Volume 4, Number 1, [1987]

Date: 1987
Creator: North Texas State University
Description: ReSource magazine includes articles and notes about research at North Texas State University in various academic fields.
Contributing Partner: University Relations, Communications & Marketing department for UNT
The North Texan, Volume 46, Number 1, Spring 1996

The North Texan, Volume 46, Number 1, Spring 1996

Date: Spring 1996
Creator: University of North Texas
Description: The North Texan includes articles and notes about University of North Texas students, faculty, and alumni activities.
Contributing Partner: University Relations, Communications & Marketing department for UNT
Unique applications of cultured neuronal networks in pharmacology, toxicology, and basic neuroscience

Unique applications of cultured neuronal networks in pharmacology, toxicology, and basic neuroscience

Date: May 2001
Creator: Keefer, Edward W.
Description: This dissertation research explored the capabilities of neuronal networks grown on substrate integrated microelectrode arrays in vitro with emphasis on utilizing such preparations in three specific application domains: pharmacology and drug development, biosensors and neurotoxicology, and the study of burst and synaptic mechanisms. Chapter 1 details the testing of seven novel AChE inhibitors, demonstrating that neuronal networks rapidly detect small molecular differences in closely related compounds, and reveal information about their probable physiological effects that are not attainable through biochemical characterization alone. Chapter 2 shows how neuronal networks may be used to classify and characterize an unknown compound. The compound, trimethylol propane phosphate (TMPP) elicited changes in network activity that resembled those induced by bicuculline, a known epileptogenic. Further work determined that TMPP produces its effects on network activity through a competitive inhibition of the GABAA receptor. This demonstrates that neuronal networks can provide rapid, reliable warning of the presence of toxic substances, and from the manner in which the spontaneous activity changes provide information on the class of compound present and its potential physiological effects. Additional simple pharmacological tests can provide valuable information on primary mechanisms involved in the altered neuronal network responses. Chapter 3 explores the effects produced ...
Contributing Partner: UNT Libraries
Neuronal Network Analyses in vitro of Acute Individual and Combined Responses to Fluoxetine and Ethanol

Neuronal Network Analyses in vitro of Acute Individual and Combined Responses to Fluoxetine and Ethanol

Date: August 2002
Creator: Xia,Yun
Description: Embryonic murine neuronal networks cultured on microelectrode arrays were used to quantify acute electrophysiological effects of fluoxetine and ethanol. Spontaneously active frontal cortex cultures showed highly repeatable, dose-dependent sensitivities to both compounds. Cultures began to respond to fluoxetine at 3 µM and were shut off at 10-16 µM. EC50s mean ± S.D. for spike and burst rates were 4.1 ± 1.5 µM and 4.5 ± 1.1 µM (n=14). The fluoxetine inhibition was reversible and without effect on action potential wave shapes. Ethanol showed initial inhibition at 20 mM, with spike and burst rate EC50s at 52.0 ± 17.4 mM and 56.0 ± 17.0 mM (n=15). Ethanol concentrations above 100 -140 mM led to cessation of activity. Although ethanol did not change the shape and amplitude of action potentials, unit specific effects were found. The combined application of ethanol and fluoxetine was additive. Ethanol did not potentiate the effect of fluoxetine.
Contributing Partner: UNT Libraries
Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure

Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure

Date: August 2010
Creator: Colbert, Crystal
Description: Polycyclic aromatic hydrocarbons (PAHs) are a well studied and diverse class of environmental toxicants. PAHs act via the aryl hydrocarbon receptor (AhR), and studies have suggested that PAHs may elicit neurological and estrogenic effects. Doses of PAHs between 50 to 150 ppm may elicit neurotoxicity in rodent models. The present study investigated the effects of naphthalene on in vivo steroidogenesis in Swiss Webster male mice, and in vitro neural function of Balb-C/ICR mice frontal cortex neurons. These data suggest that naphthalene may not elicit steroidogenic effects at concentrations ranging from 0.2 to 25 mg/kg/day, following a 7 day subcutaneous dosing regime. In addition, naphthalene may cause functional toxicity of frontal cortex neurons at concentrations of 32 to 160 ppm naphthalene.
Contributing Partner: UNT Libraries
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