FLP-mediated conditional loss of an essential gene to facilitate complementation assays
Description:
Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious and time-consuming step, the newly developed two-component system utilizes a site-specific recombinase to excise a wild-type copy of the gene of interest from transformed tissues. In the first componen…
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Date:
December 2007
Creator:
Ganesan, Savita
Partner:
UNT Libraries