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Water potential and starvation stress in deep subsurface microorganisms

Description: Nine intact core samples, collected aseptically from depths of 10--436 m near the Savannah River Plant in South Carolina, were tested for water potential, microbial numbers, and microbial activity. Although all samples were collected from below the water table, two samples (a Pee Dee clay from 238 m and a Middendorf clay from 324 m) showed unsaturated conditions ({minus}2.7 and {minus}2.1 MPa, respectively). Both of these samples had very low numbers of culturable cells, low microbial biomass (… more
Date: December 31, 1990
Creator: Kieft, Thomas L.; Rosacker, Laurie L.; Willcox, David & Franklin, Abraham J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Water Potential and Starvation Stress in Deep Subsurface Microorganisms

Description: Nine intact core samples, collected aseptically from depths of 10--436 m near the Savannah River Plant in South Carolina, were tested for water potential, microbial numbers, and microbial activity. Although all samples were collected from below the water table, two samples (a Pee Dee clay from 238 m and a Middendorf clay from 324 m) showed unsaturated conditions ({minus}2.7 and {minus}2.1 MPa, respectively). Both of these samples had very low numbers of culturable cells, low microbial biomass (… more
Date: January 1, 1990
Creator: Kieft, Thomas L.; Rosacker, Laurie L.; Willcox, David & Franklin, Abraham J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Environmental Parameters Controlling Microbial Activities in Terrestrial Subsurface Environments. Technical Completion Report.

Description: This project was begun in July 1988 as part of Phase I of the Deep Microbiology Subprogram. At this time, the Subprogram was preparing for sampling near the Savannah River Site (SRS) from what was being termed the ``Investigator`s Hole.`` This was the fourth hole drilled for sampling in the coastal plain sediments at a site near the SRS. Since there was a possibility of sampling from the saline Triassic basin in the deeper regions in this fourth hole, there was particular interest in quantifyin… more
Date: December 31, 1990
Creator: Kieft, Thomas L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Environmental Parameters Controlling Microbial Activities in Terrestrial Subsurface Environments

Description: This project was begun in July 1988 as part of Phase I of the Deep Microbiology Subprogram. At this time, the Subprogram was preparing for sampling near the Savannah River Site (SRS) from what was being termed the Investigator's Hole.'' This was the fourth hole drilled for sampling in the coastal plain sediments at a site near the SRS. Since there was a possibility of sampling from the saline Triassic basin in the deeper regions in this fourth hole, there was particular interest in quantifying … more
Date: January 1, 1990
Creator: Kieft, Thomas L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Factors Limiting Microbial Activity in Volcanic Tuff at Yucca Mountain

Description: Samples of tuff aseptically collected from 10 locations in the Exploratory Shaft Facility at the site of the proposed high-level nuclear waste repository at Yucca Mountain, Nevada Test Site were analyzed for microbiological populations, activities, and factors limiting microbial activity. Radiotracer assays ({sup 14}C-labeled organic substrate mineralization), direct microscopic counts, and plate counts were used. Radiolabeled substrates were glucose, acetate, and glutamate. Radiotracer experim… more
Date: September 1, 1996
Creator: Kieft, Thomas L.; Kovacik, William P. & Taylor, Jennifer
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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