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  Partner: UNT College of Arts and Sciences
 Department: Biological Sciences
Rapid Analysis of Legume Root Nodule Development Using Confocal Microscopy

Rapid Analysis of Legume Root Nodule Development Using Confocal Microscopy

Date: 2004
Creator: Haynes, Janine G.; Czymmek, Kirk J.; Carlson, Carol A.; Veereshlingam, Harita; Dickstein, Rebecca & Sherrier, D. Janine
Description: This article discusses the rapid analysis of legume root nodule development using confocal microscopy. A rapid method for detailed analysis of nodule formation has been developed. Inoculated root tissues were stained with SYTO 13, a cell-permeant fluorescent nucleic acid-binding dye, and visualized using confocal laser scanning microscopy (CLSM). Structures with high concentrations of DNA and RNA, such as plant cell nuclei and bacteria, labeled strongly. The autofluorescent properties of cell walls made it possible to use CLSM to visualize both plant and rhizobial structures and generate a three-dimensional reconstruction of the root and invading bacteria. This method allowed clear observation of stages and structures important in nodule formation, such as rhizobial attachment to root hairs, hair deformation, infection thread ramification, nodule primordium development and nodule cell invasion. Bacteroid structures were easily were easily assessed without the need for fixation that might alter cellular integrity. Plant nodulation mutants with phenotypic differences in thread growth, cellular invasion and plant defense response were also documented. Multiple samples can be assessed using detailed microscopy without the need for extensive preparative work, labor-intensive analysis, or the generation of genetically modified samples.
Contributing Partner: UNT College of Arts and Sciences
Remote Sensing and GIS for Nonpoint Source Pollution Analysis in the City of Dallas' Eastern Watersheds

Remote Sensing and GIS for Nonpoint Source Pollution Analysis in the City of Dallas' Eastern Watersheds

Date: June 1989
Creator: University of North Texas. Dept. of Biological Sciences
Description: This report describes the findings of a study conducted on the Eastern Watersheds of Lake Lavon, Lake Ray Hubbard, Lake Tawakoni, Lake Palestine and Lake Fork, which are located within the Blackland Prairie, Post Oak Savannah and Pineywoods provinces. These watersheds are among nine that provide drinking water to Dallas, Texas. The study examines the potential benefit of "remote sensing and geographic information systems (GIS) for watershed management" in these five watersheds (p. iii).
Contributing Partner: UNT College of Arts and Sciences
Remote Sensing and GIS for Nonpoint Source Pollution Analysis in the City of Dallas' Western Watersheds

Remote Sensing and GIS for Nonpoint Source Pollution Analysis in the City of Dallas' Western Watersheds

Date: August 1988
Creator: University of North Texas. Dept. of Biological Sciences
Description: This report describes the findings of a study conducted on the watersheds of "Lake Lewisville, Lake Ray Roberts, Lake Grapevine and the Elm Fork of the Trinity River between Lake Lewisville and Frazier Dam," which are all part of the upper Trinity drainage basin (p. 31). The study examines the potential benefit of "remote sensing and geographic information systems (GIS) for watershed management" in and around Dallas, Texas (p. i).
Contributing Partner: UNT College of Arts and Sciences
Response to: Use of prior odds for missing persons identifications - author's reply

Response to: Use of prior odds for missing persons identifications - author's reply

Date: February 1, 2012
Creator: Budowle, Bruce; Ge, Jianye; Chakraborty, Ranajit & Gill-King, Harrell
Description: This article is in response to an authors' reply to 'Use of prior odds for missing persons identifications.' This response is to the reply by Alex Biedermann, Franco Taroni, and Pierre Margot.
Contributing Partner: UNT College of Arts and Sciences
RICHEST - a web server for richness estimation in biological data

RICHEST - a web server for richness estimation in biological data

Date: February 27, 2009
Creator: Durden, Chris & Dong, Qunfeng
Description: This article discusses RICHEST, a web server for richness estimation in biological data. Richness is defined as the number of distinct species or classes in a sample or population. Although richness estimation is an important practice, it requires mathematical and computational methods that are challenging to understand and implement. The authors have developed a web server, RICHness ESTimator (RICHEST), which implements three non-parametric statistical methods for richness estimation. Its user-friendly web interface allows users to analyze and compare their data conveniently over the web.
Contributing Partner: UNT College of Arts and Sciences
Role of Hypoxia in the Evolution and Development of the Cardiovascular System

Role of Hypoxia in the Evolution and Development of the Cardiovascular System

Date: 2007
Creator: Fisher, Steven A. & Burggren, Warren W.
Description: This article discusses the role of hypoxia in the evolution and development of the cardiovascular system. How multicellular organisms obtain and use oxygen and other substrates has evolved over hundreds of millions of years in parallel with the evolution of oxygen-delivery systems. A steady supply of oxygen is critical to the existence of organisms that depend on oxygen as a primary source of fuel (i.e., those that live by aerobic metabolism). Not surprisingly, a number of mechanisms have evolved to defend against oxygen deprivation. This review highlights evolutionary and developmental aspects of O(2) delivery to allow understanding of adaptive responses to O(2) deprivation (hypoxia). First, the authors consider how the drive for more efficient oxygen delivery from the heart to the periphery may have shaped the evolution of the cardiovascular system, with particular attention to the routing of oxygenated and deoxygenated blood in the cardiac outlet. Then the authors consider the role of O(2) in the morphogenesis and the cardiovascular system of animals of increasing size and complexity. The authors conclude by suggesting areas for future research regarding the role of oxygen deprivation and oxidative stress in the normal development of the heart and vascular or in the pathogenesis of ...
Contributing Partner: UNT College of Arts and Sciences
A self-adaptive burst-detection algorithm

A self-adaptive burst-detection algorithm

Date: July 6, 2007
Creator: Tam, Nicoladie D.
Description: This article accompanies a poster presentation on a self-adaptive burst-detection algorithm. A self-adaptive, time-scale invariant single-unit spike train analysis technique is introduced to detect burst firings in neurons. This burst-detection method is an adaptive algorithm that uses the characteristic firing patterns statistics within and between bursts to identify the inter-burst period, intra-burst period and burst duration.
Contributing Partner: UNT College of Arts and Sciences
Symplastic Continuity between Companion Cells and the Translocation Stream: Long-Distance Transport Is Controlled by Retention and Retrieval Mechanisms in the Phloem

Symplastic Continuity between Companion Cells and the Translocation Stream: Long-Distance Transport Is Controlled by Retention and Retrieval Mechanisms in the Phloem

Date: April 2003
Creator: Ayre, Brian G.; Keller, Felix & Turgeon, Robert
Description: This article discusses symplastic continuity between companion cells and the translocation stream. Substantial symplastic continuity appears to exist between companion cells (CCs) and sieve elements of the phloem, which suggests that small solutes within the CC are subject to indiscriminate long-distance transport via the translocation stream. To test this hypothesis, the distributions of exotic and endogenous solutes synthesized in the CCs of minor veins were studied. Octopine, a charged molecule derived from arginine and pyruvate, was efficiently transported through the phloem but was also transferred in substantial amounts to the apoplast, and presumably other non-phloem compartments. The disaccharide galactinol also accumulated in non-phloem compartments, but long-distance transport was limited. Conversely, sucrose, raffinose, and especially stachyose demonstrated reduced accumulation and efficient transport out of the leaf. The authors conclude that small metabolites in the cytosol of CCs do enter the translocation stream indiscriminately but are also subject to distributive forces, such as nonselective and carrier-mediated membrane transport and symplastic dispersal, that may effectively clear a compound from the phloem or retain it for long-distance transport. A model is proposed in which the transport or oligosaccharides is an adaptive strategy to improve photoassimilate retention, and consequently translocation efficiency, the phloem.
Contributing Partner: UNT College of Arts and Sciences
TableMaker: An ad hoc Query Tool for Relational Databases

TableMaker: An ad hoc Query Tool for Relational Databases

Date: July 2008
Creator: Lushbough, Carol; Duvick, Jon; Dong, Qunfeng; Jennewein, Douglas; Reynoldson, Joe & Brendel, Volker
Description: This paper discusses an ad hoc query tool for relational databases. Most Web servers hosting biological data limit users to a defined set of search options and output formats that are short of the whole range of options available to users with direct database access. However, to make full use of the wealth of data in the database resource, it is desirable to have an intermediate solution that provides a broad range of flexible query and output options through a Web portal.
Contributing Partner: UNT College of Arts and Sciences
Temporal associative memory (TAM) by spike-timing dependent plasticity

Temporal associative memory (TAM) by spike-timing dependent plasticity

Date: July 2010
Creator: Tam, Nicoladie D.
Description: This article accompanies a poster presentation on temporal associative memory (TAM). Spike-timing synaptic plasticity (STDP) describes the increase and decrease in synaptic strength depending on the relative arrival time of the pre- and post-synaptic spikes. The relative timing that determines whether the synapse is strengthened by long-term potentiation (LTP), or weakened by long-term depression (LTD) was first reported experimentally and computationally. Because of the asymmetry in timing, it is also called "temporally asymmetric Hebbian plasticity" theoretically. Numerous studies on STDP had explored the biological mechanisms for induction of LTP and LTD. This paper will focus on the computational function of STDP in the formation of temporal associative memory (TAM).
Contributing Partner: UNT College of Arts and Sciences