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- Acculturation and Sociocultural Influences as Predictors of Family Relationships and Body Image Dissatisfaction in African American, Hispanic American, and European American Women
- Ethnic differences in etiological factors linked to body image dissatisfaction and eating disorders were examined. In addition, the interaction of acculturation and body image dissatisfaction in influencing minority women's relationships with their parents was investigated. Participants consisted of 302 undergraduates from three ethnic groups: Caucasian, Hispanic American, and African American women who were administered self-report measures. Differences were not found between the groups in body image dissatisfaction. Low self-esteem, internalization of the thin ideal, and family emphasis on weight and appearance were all related to more body image dissatisfaction for each of these groups; however, differences in degree of endorsement were also noted between the ethnic groups on these factors. Based on the interaction findings (body image x acculturation) separation from one's mother was found in the area of attitudes and emotions for the Hispanic sample but not for the African American sample on any of the parent scales. Areas for future research and implications for diagnosis and treatment of minority populations are also discussed. digital.library.unt.edu/ark:/67531/metadc5463/
- Acculturation Level, Generational Status and Gender: Their Role in Acculturative Stress in Young Adolescent Mexican Americans
- The purpose of this study was to determine relationships between acculturation level, generational status, and gender with acculturative stress. Acculturation level was determined by the Acculturation Rating Scale for Mexican Americans-II (ARSMA-II) and acculturative stress was determined by the Societal, Attitudinal, Familial and Environmental Acculturative Stress Scale-Children's Version (SAFE-C). Subjects included 1268 Hispanic children ages 11-15. In order to validate the usefulness of the ARSMA-II with this sample, analyses were conducted between acculturation level and generational status. The Pearson product moment correlation (r=.44) and the ANOVA between the mean acculturation score and generational status were significant. However, the mean acculturation score from this study was considerably lower than the ARSMA-II score; therefore, new acculturation levels were developed to establish local adolescent norms for the ARSMA-II. All analyses involving acculturation levels were conducted using both the ARSMA-II and new acculturation levels because 300 subjects were reclassified with the new norms. Significant results were similar using both acculturation levels; however, there were more between group differences using the new acculturation levels. It was hypothesized that as acculturation level increased toward the Anglo culture, acculturative stress would decrease. The one-way ANOVA confirmed this relationship. It was also hypothesized that as generational status increased, acculturative stress would decrease. A one-way ANOVA also supported this hypothesis. In order to replicate previous findings on gender, a one-way ANOVA was conducted with acculturative stress and acculturation level. Results for both were non-significant. Overall findings indicate that generational status and acculturation level have a significant impact on acculturative stress in Hispanic children; however, gender does not seem to be a factor. Findings emphasize the importance of addressing cultural issues in the assessment, intervention, and treatment of acculturating Hispanic children. Furthermore, the ARSMA-II appears to be a useful instrument in assessing acculturation level in young adolescent Hispanics though new local adolescent norms for the ARSMA-II were developed from this study. digital.library.unt.edu/ark:/67531/metadc4592/
- Acculturation, Parental Control, and Adjustment among Asian Indian Women
- The present study examines the relationship between acculturation, parental control, and psychological adjustment among adult first and second-generation Asian Indian women who have immigrated, or whose parents have immigrated to the United States, from the Indian state of Kerala. Data from 73 participants indicate second-generation immigrants report poorer psychological adjustment than do their counterparts. Additionally, regression analyses reveal discomfort towards Kerala culture significantly predicts depressive symptoms, while high maternal control predicts self-esteem. Qualitative data were collected to provide richer understanding of immigrants' adaptation to the U.S. Implications of this research may impact mental health practitioners' ability to improve quality of life with Asian Indian women from Kerala. digital.library.unt.edu/ark:/67531/metadc3600/
- Acculturative Processes and Their Impact on Self-Reports of Psychological Distress in Mexican-American Adolescents
- The current study examined the effects of acculturative processes on the self-report of behavioral problems in Hispanic children ages 11-14. Acculturation was measured by the Acculturation Rating Scale for Mexican Americans-II (ARSMA-II) (ã Sage Publications, Thousand Oaks, CA, www.sagepub.com) (Cuellar, Arnold, and Maldonado, 1995) and the self-report of behavioral symptoms was assessed using the Youth Self-Report (ã T.M. Achenbach, Burlington, VT, www.aseba.com) (Achenbach, 1991). It was hypothesized that while both the linear and orthogonal categories of acculturation would account for a significant proportion of the variance in behavior problems in this age group, the orthogonal model would account for a larger proportion of variance due to its multidimensional nature. As well, it was hypothesized that the experimental Marginalization scales of the ARSMA-II would be predictive of behavioral problems. Multivariate analysis of variance was used to test these hypotheses and results were non-significant for the linear, orthogonal, and marginalization categories. The effects of the ethnic/cultural homogeneity of the region from which the sample was drawn, the buffering of social support, and the developmental aspects of ethnic identity are discussed as factors which may have influenced the potential impact of acculturative stress on psychological and behavioral functioning. digital.library.unt.edu/ark:/67531/metadc4217/
- Accuracy and Efficiency in Computational Chemistry: The Correlation Consistent Composite Approach
- One of the central concerns of computational chemistry is that of efficiency (i.e. the development of methodologies which will yield increased accuracy of prediction without requiring additional computational resources – RAM, disk space, computing time). Though the equations of quantum mechanics are known, the solutions to these equations often require a great deal of computing power. This dissertation primarily concerns the theme of improved computational efficiency (i.e. the achievement of greater accuracy with reduced computational cost). Improvements in the efficiency of computational chemistry are explored first in terms of the correlation consistent composite approach (ccCA). The ccCA methodology was modified and this enhanced ccCA methodology was tested against the diverse G3/05 set of 454 energetic properties. As computational efficiency improves, molecules of increasing size may be studied and this dissertation explored the issues (differential correlation and size extensivity effects) associated with obtaining chemically accurate (within 1 kcal mol-1) enthalpies of formation for hydrocarbon molecules of escalating size. Two applied projects are also described; these projects concerned the theoretical prediction of a novel rare gas compound, FKrOH, and the mechanism of human glutathione synthetase’s (hGS) negative cooperativity. The final work examined the prospect for the parameterization of the modified embedded atom method (MEAM) potential using first principles calculations of dimer and trimer energies of nickel and carbon systems. This method of parameterization holds promise for increasing the accuracy of simulations for bulk properties within the field of materials science. digital.library.unt.edu/ark:/67531/metadc84300/
- Accuracy of Self-Reported Height, Weight, and Calculated BMI and Resulting FITNESSGRAM® Healthy Fitness Zone Classification
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The determination of adiposity in adolescents is often assessed with calculations of body mass indices (BMI). Researchers often obtain these measurements from self-reported (SR) values. The purpose of this study was to determine the accuracy of SR height, weight, and calculated BMI (from height and weight). SR and actual measured (ME) BMI values were compared with standards from the FITNESSGRAM® Healthy Fitness Zone (HFZ) classifications. SR height and calculated BMI were found to be accurate while SR weight was, on average, underreported by 4.77 lbs. Because of these errors in SR height and weight, accuracy of classification into the FITNESSGRAM® HFZ was compromised. Consequently, it is important that researchers ascertain actual values of height and weight when measuring adolescents rather than use those from self-reports. digital.library.unt.edu/ark:/67531/metadc5214/ - The Accuracy of Teacher Rating of Pupil Personality and Achievement as Compared with Standardized Test Scores
- This study has the following purpose: (1) to find the relationship between teacher rating of pupil personality and standardized test results; (2) to find the relationship between teacher rating of pupil achievement and standardized test results; and (3) to determine where more emphasis should be placed in order to better evaluate the child and his work. digital.library.unt.edu/ark:/67531/metadc70293/
- Accuracy variations in human facial identification based on time of exposure.
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Access: Use of this item is restricted to the UNT Community.
This study examined the relationship between time of exposure to the human face and accurate subsequent photo line-up identification. A volunteer group of 124 undergraduate students was divided into three approximately equal sized subgroups. The three groups were then exposed to a video or a portion of a video depicting a theft. Exposure times ranged from two minutes to 30 seconds. The subjects were then given a questionnaire and shown a photo line-up of the mock perpetrator and five foils. Subjects were asked to identify the perpetrator and mark that identification on the questionnaire. Results of the experiment indicated that the longer a subject was exposed the greater the possibility of an accurate identification. digital.library.unt.edu/ark:/67531/metadc5415/ - Acting Ethically: Behavior and the Sustainable Society
- One of the most important factors for creating the sustainable society is that the individuals in that society behave in an environmentally sustainable fashion. Yet achieving appropriate behavior in any society is difficult, and the challenge is no less with regards to sustainability. Three of the most important factors for determining behavior have recently been highlighted by psychologists: personal efficacy, social influence, and internal standards. Because these three factors play a prominent role in behavior, it is necessary to examine what role they play in creating sustainability and how they may be utilized to achieve optimal behavior patterns. Ultimately, in order to achieve sustainability solutions must focus on individual action, realistic governmental regulation, and sustained, direct encounters with the natural world. While much time and energy has been spent on social influence and personal efficacy, less has been devoted to internal standards and this area needs more attention if there is to be any realistic attempt at creating proper behavior patterns. digital.library.unt.edu/ark:/67531/metadc3916/
- The Actions and Operational Thinking of Generals Stratemeyer and Partridge during the Korean War: Adjusting to Political Restrictions of Air Campaigns
- Airpower played an important supporting role in the Korean War, and as General of the Army Douglas MacArthur pursued victory in the war and President Harry S Truman's objectives altered throughout the first year of the conflict, tension arose between the two men. One issue in these frictions was the restriction of airpower. Not only MacArthur, but also his admiring subordinate Lieutenant General George E. Stratemeyer commanding the Far East Air Forces, and Fifth Air Force commander Major General Earle E. Partridge opposed the restrictions which had been imposed on airmen from the outset of the conflict. Stratemeyer did so partly because of his loyalty to MacArthur, who wanted latitude in coping with the situation in the field and defeating the Communist enemy. Partridge did so because he thought they endangered his personnel and limited the effectiveness of airpower in the war. These commanders had a fundamentally different opinion from Washington regarding the likelihood of overt Soviet intervention in the war, and because they did not think the Korean War would become a world war, they were more willing than Washington to prosecute the war more aggressively. MacArthur's conflict ended with his removal in April 1951, and Stratemeyer (who suffered a heart attack weeks afterward) continued to advocate for forceful American foreign policy in Asia during his retirement. Partridge eventually earned four stars and long after the war likewise continued to disfavor the restrictions which had been put in place. Between oral history interviews in 1974 and 1978, however, Partridge reconsidered the issue of restrictions. He expressed that the Korean War had been a considerable challenge without a wider war, implying that restrictions had perhaps been important. digital.library.unt.edu/ark:/67531/metadc6056/
- Actions Taken by Texas School Districts to Prevent Fraud
- This research is a descriptive analysis answering the question, what measures are currently taken by the leadership―boards of trustees and superintendents of schools―of Texas school districts to prevent embezzlement? The research perspective utilized was quantitative with a descriptive, cross-sectional design. Data collection was accomplished through a survey with questions constructed from the most commonly recommended strategies discovered through the review of literature. The survey was distributed to the 1031 superintendents of school districts in Texas via email. The response rate was 33% or 339 returned surveys. The data set created concentrates on the four most common preventive measures: policy and procedure, management, auditing, and ethics. These measures are considered as they function to interrupt the principles of the fraud triangle. Comparisons were completed regarding region, district size, superintendent tenure and superintendent experience. Policy adoption was found to be extremely widespread. Procedures written to fully implement policy were less prevalent. Review of management practices found problems concerning credit cards, personnel evaluations, and password access to multiple computer finance recordkeeping systems concentrated in one employee. External auditing programs were universal due to statutory mandate but internal auditors and internal audit committees were few. Ethics training for business office personnel existed but with little consistent application across districts. The adoption of a code of ethics for business office personnel was rare. Recommendations made were that school leaders should be educated concerning appropriate actions in the common prevention areas. They need an to understand the importance of internal auditing, know the language in local policy, and they need to write procedures. digital.library.unt.edu/ark:/67531/metadc68052/
- Activating Space within the Object and the Site
- I look at the world as a sculptor, examining physical constructs and implied meanings. My current research developed from my earlier studies of “containment” or, more specifically, “encapsulation,” creating visual, often physical, boundaries around selected content. Encapsulation confers a more active role than “containment”, a process rather than a result. This idea speaks to the issues of form, and asks the viewer to question the outside “shape of the form” in relation to the inside shape and content. My work focuses on exposed interior spaces and forms, allowing the viewer to enter the space physically as well as mentally and psychologically. Built in a large enough scale, the viewer could actually become the content. The sculpture’s interpretation revolves around the seen as well as the unseen. I built this duality into my work by using transparent and opaque materials. I also implemented small diameter stainless steel rod along with the transparent and opaque vinyl to reduce forms to their respective shapes and volumes. This approach allowed me to clean the “slate” of an object’s collective meaning and context, adapting it to the intent of my work. digital.library.unt.edu/ark:/67531/metadc5827/
- [The Activator], Volume 1, Number 1, October 1944
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67667/
- [The Activator], Volume 1, Number 2, November 1944
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75208/
- The Activator, Volume 1 , Number 3, December 1944
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75209/
- The Activator, Volume 1, Number 4, January 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67668/
- The Activator, Volume 1, Number 5, February 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67669/
- The Activator, Volume 1, Number 6, March 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67670/
- The Activator, Volume 1, Number 7, April 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67671/
- The Activator, Volume 1, Number 8, May 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67672/
- The Activator, Volume 2, Number 1, October 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67673/
- The Activator, Volume 2, Number 2, November 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc67674/
- The Activator, Volume 2, Number 3, December 1945
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. According to the cover, this issue includes "Abstracts of Papers Given at Texas Regional Meeting of The American Chemical Society, Dec. 7th & 8th, 1945." digital.library.unt.edu/ark:/67531/metadc67675/
- The Activator, Special Issue Program
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. This special issue includes a full program for the events of the society's regional meeting at the University of Texas as well as descriptions of local attractions and special programs. digital.library.unt.edu/ark:/67531/metadc67677/
- The Activator, Volume 2, Number 4, January 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75210/
- The Activator, Volume 2, Number 5, February 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75211/
- The Activator, Volume 2, Number 6, March 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. This is the directory issue. digital.library.unt.edu/ark:/67531/metadc75212/
- The Activator, Volume 2, Number 7, April 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75213/
- The Activator, Volume 2, Number 8, May 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75214/
- The Activator, Volume 3, Number 1, October 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75215/
- The Activator, Volume 3, Number 3, December 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. According to the cover, this issue includes "Abstracts of Papers Given at Texas Regional Meeting of The American Chemical Society, Dallas, Texas, December 12th & 13th, 1946." digital.library.unt.edu/ark:/67531/metadc75217/
- The Activator, Volume 3, Number 2, November 1946
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75216/
- The Activator, Special Issue Program
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. This special issue includes a full program for the events of the society's regional meeting at the University of Texas as well as descriptions of local attractions and special programs. digital.library.unt.edu/ark:/67531/metadc75218/
- The Activator, Volume 3, Number 4, January 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75219/
- The Activator, Volume 3, Number 5, February 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75220/
- The Activator, Volume 3, Number 6, March 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75221/
- The Activator, Volume 3, Number 7, April 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75222/
- The Activator, Volume 3, Number 8, May 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75223/
- The Activator, Volume 4, Number 1, October 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75224/
- The Activator, Volume 4, Number 2, November 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75225/
- The Activator, Volume 4, Number 3, December 1947
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75226/
- The Activator, Volume 4, Number 4, January 1948
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75227/
- The Activator, Volume 4, Number 5, February 1948
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75228/
- The Activator, Volume 4, Number 6, March 1948
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75229/
- The Activator, Volume 4, Number 7, April 1948
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75230/
- The Activator, Volume 4, Number 8, May 1948
- This publication of the Dallas-Fort Worth Section of the American Chemical Society includes information about research, prominent scientist, organizational business, and various other stories of interest to the community. Published monthly during long academic semesters. digital.library.unt.edu/ark:/67531/metadc75231/
- Active Climate Legislative Commissions and Executive Branch Advisory Groups
- This map shows states with government bodies that study the impacts of climate change and recommend policies for mitigation and adaptation. digital.library.unt.edu/ark:/67531/metadc31120/
- Activities and Experiences of Academic Librarians Embedded in Online Courses
- This presentation discusses activities and experiences of academic librarians embedded in online courses. The purpose is to determine common practices and assess various experiences. digital.library.unt.edu/ark:/67531/metadc67611/
- An actor-partner interdependence model of attachment processes, conflict resolution, and psychological abuse on relationship quality in a community sample of heterosexual couples.
- The purpose of this study is to determine whether adult attachment style, psychological abuse in the marriage, conflict resolution strategies, and gender are associated with relational quality in childless couples in the early years of their marriage. Data were collected from 92 married couples who were recruited from university campuses, churches, and community organizations through e-mails, flyers, newspaper advertisements and mailings. Conceptualizing the interdependence of dyadic data from the actor-partner interdependence model (APIM), multilevel linear modeling (MLM) was used to analyze differences within and between couples. It was hypothesized that higher levels of attachment anxiety or avoidance, psychological abuse, and maladaptive conflict resolution strategies would be associated with lower relational quality. Results indicated that attachment avoidance had stronger associations with relational quality than did attachment anxiety, and that higher levels of attachment avoidance were associated with lower relational quality. Additionally, findings indicated a direct negative relationship between both actor and partner psychological abuse and the actor's relational quality. The discussion section addresses strengths and limitations of the present study as well as directions for future research. digital.library.unt.edu/ark:/67531/metadc9932/
- N-Acylethanolamine metabolism during seed germination: Molecular identification of a functional N-acylethanolamine amidohydrolase.
- N-Acylethanolamines (NAEs) are endogenous lipid metabolites that occur in a variety of dry seeds, and their levels decline rapidly during the first few hours of imbibition (Chapman et al., 1999, Plant Physiol., 120:1157-1164). Biochemical studies supported the existence of an NAE amidohydrolase activity in seeds and seedlings, and efforts were directed toward identification of DNA sequences encoding this enzyme. Mammalian tissues metabolize NAEs via an amidase enzyme designated fatty acid amide hydrolase (FAAH). Based on the characteristic amidase signature sequence in mammalian FAAH, a candidate Arabidopsis cDNA was identified and isolated by reverse transcriptase-PCR. The Arabidopsis cDNA was expressed in E. coli and the recombinant protein indeed hydrolyzed a range of NAEs to free fatty acids and ethanolamine. Kinetic parameters for the recombinant protein were consistent with those properties of the rat FAAH, supporting identification of this Arabidopsis cDNA as a FAAH homologue. Two T-DNA insertional mutant lines with disruptions in the Arabidopsis NAE amidohydrolase gene (At5g64440) were identified. The homozygous mutant seedlings were more sensitive than the wild type to exogenously applied NAE 12:0. Transgenic seedlings overexpressing the NAE amidohydrolase enzyme showed noticeably greater tolerance to NAE 12:0 than wild type seedlings. These results together provide evidence in vitro and in vivo for the molecular identification of Arabidopsis NAE amidohydrolase. Moreover, the plants with altered NAE amidohydrolase expression may provide new tools for improved understanding of the role of NAEs in germination and seedling growth. digital.library.unt.edu/ark:/67531/metadc4575/