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  Partner: UNT Libraries
 Degree Discipline: Biology
 Collection: UNT Theses and Dissertations
Improved Fabrication and Quality Control of Substrate Integrated Microelectrode Arrays

Improved Fabrication and Quality Control of Substrate Integrated Microelectrode Arrays

Date: May 2000
Creator: Zim, Bret E.
Description: Spontaneously active monolayer neuronal networks cultured on photoetched multimicroelectrode plates (MMEPs) offer great potential for use in studying neuronal networks. However, there are many problems associated with frequent, long-term use of MMEPs. The major problems include (1) polysiloxane insulation deterioration and breakdown, (2) and loss of gold at the gold electroplated indium-tin oxide (ITO) electrodes. The objective of this investigation was to correct these major problems. Quality control measures were employed to monitor MMEP fabrication variables. The phenotypes of polysiloxane degradation were identified and classified. Factors that were found to contribute most to insulation deterioration were (1) moisture contamination during MMEP insulation, (2) loss of the quartz barrier layer from excessive exposure to basic solutions, and (3) repetitive use in culture. As a result, the insulation equipment and methods were modified to control moisture-dependent insulation deterioration, and the KOH reprocessing solution was replaced with tetramethylguanidine to prevent damage to the quartz. The problems associated with gold electroplating were solved via the addition of a pulsed-DC application of gold in a new citrate buffered electroplating solution.
Contributing Partner: UNT Libraries
The In Vitro Bactericidal Activity of Antiserum Against Pseudomonas Aeruginosa

The In Vitro Bactericidal Activity of Antiserum Against Pseudomonas Aeruginosa

Date: May 1972
Creator: Stanford, Shirley A.
Description: The purpose of this research was to attempt to duplicate the in vitro bactericidal test against Pseudomonas aeruginosa as reported by Bjornson and Michael and to correlate agglutinating titer of an antiserum to protective activity.
Contributing Partner: UNT Libraries
In vitro Cultures of Morus alba for Enhancing Production of Phytoestrogens

In vitro Cultures of Morus alba for Enhancing Production of Phytoestrogens

Date: December 2009
Creator: Bakshi, Vibhu
Description: Plant estrogens have long been associated with health benefits. The potential of tissue culture techniques for the production of several secondary metabolites has been known for many years. Tissue cultures stimulate the production or induce the biosynthesis of novel compounds not found in the mature plant. Tissue culture of Morus alba, family Moraceae, is known to contain phytoestrogens, was established on plant-hormone supplemented Murashige and Skoog (MS) medium. Petiole and the stem tissue from mature trees were the best explants for initiation and proliferation of calli. The best callus proliferation was obtained on MS medium containing 1-napthalene acetic acid (1mg/ml) and benzylaminopurine (0.5mg/ml) for M. alba. Comparison of phytoestrogens of Moraceae species from in vivo and in vitro tissue isolation were carried out. The estrogenic activities of callus extracts were assayed in an estrogen-responsive yeast system expressing the human estrogen receptor alpha. Male callus extracts had higher estrogenic activity than male and female extracts from in vivo and in vitro tissues. Isolation and characterization of phytoestrogens from above tissues were carried out using solid phase extraction, high perfomance liquid chromatography and mass spectrometry techniques. Biochanin A, an isoflavonoid, was isolated as one of the compounds in male callus extracts. Biochanin ...
Contributing Partner: UNT Libraries
In Vitro Determination of the Cellulose-Decomposing Rates of Twelve Denton County, Texas Soils

In Vitro Determination of the Cellulose-Decomposing Rates of Twelve Denton County, Texas Soils

Date: 1950
Creator: Heather, Carl D.
Description: In this study twelve types of top soil were collected under aseptic conditions. The cellulose-decomposing rates of these were compared in order to determine the relative rates in the cellulose-decomposing potential of the microorganisms involved. Furthermore, this investigation is designed to acquire pertinent information on the rate at which natural cellulose materials are returned to available plant food.
Contributing Partner: UNT Libraries
The In Vitro Interaction of 3-Methylcholanthrene with Deoxyribonucleic Acid

The In Vitro Interaction of 3-Methylcholanthrene with Deoxyribonucleic Acid

Date: August 1967
Creator: Chapel, J. Frederick
Description: The purpose of this thesis is to report the interaction of aromatic hydrocarbons with DNA and to attempt to determine the relative binding affinities. The effect of the hydrocarbons on the continuity of the DNA molecule has been studied also and discussed.
Contributing Partner: UNT Libraries
The Incidence of Colon Bacteria on the Hands of North Texas State Teachers College Students

The Incidence of Colon Bacteria on the Hands of North Texas State Teachers College Students

Date: 1946
Creator: Hooper, Eugene
Description: This investigation has for its aim the determination, in a measure, of the degree of personal sanitation existent among students at North Texas State Teachers College, Denton, Texas.
Contributing Partner: UNT Libraries
The Incidence of Escherichia Coli and other Coliform Organisms on Seventy-One Samples of Fresh Fruits and Vegetables Purchased from Eight Grocery Stores in Denton, Texas

The Incidence of Escherichia Coli and other Coliform Organisms on Seventy-One Samples of Fresh Fruits and Vegetables Purchased from Eight Grocery Stores in Denton, Texas

Date: 1945
Creator: Scoggin, Frances Grammar
Description: This investigation has for its aim the explanation of the incidence of colon bacteria on a number of food samples purchased in grocery stores of Denton, Texas, by an analysis of correlative sanitary factors.
Contributing Partner: UNT Libraries
Incubation humidity as an environmental stressor on the osmoregulatory developmental program of the chicken, Gallus gallus domesticus.

Incubation humidity as an environmental stressor on the osmoregulatory developmental program of the chicken, Gallus gallus domesticus.

Date: August 2009
Creator: Bolin, Greta M.
Description: Fetal programming results from stressors during fetal development and may influence the occurrence of disease later in life. Maternal nutritional status and/or environment can affect renal development by inducing limited nephron endowment at birth, which results in diseases such as hypertension and coronary heart disease in mammals. Birds are likely to be effective models for this process because, like mammals, they have high pressure cardiovascular systems, mammalian-type nephrons and are homeothermic. This project uses the chicken embryo to explore physiological responses of disrupted hydration state thereby providing insights into renal fetal programming. Under normal conditions the chorioallantoic membrane (CAM) and developing avian kidney work in unison to ensure a proper balance of ions and water within the egg. White leghorn chicken eggs were incubated at 37.5oC±0.5oC and either <35%, 55-60% (normal) or >85% relative humidity. Amniotic fluid serves as the drinking source for the embryo late in development; its composition is important to salt and water homeostasis. High amniotic fluid osmolality increased the blood osmolality for embryos exposed to low humidity incubation thereby indirectly influencing the renal developmental program of the embryos from this group. Indeed estimated filtering capacity was doubled in the low humidity group (6.77 ± 0.43 mm3) ...
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Individual, Social, and Seasonal Behavior of the Thirteen-Lined Ground Squirrel (Spermophilus tridecemlineatus)

Individual, Social, and Seasonal Behavior of the Thirteen-Lined Ground Squirrel (Spermophilus tridecemlineatus)

Date: January 1970
Creator: Wistrand, Harry E.
Description: The purpose of this study is to provide a qualitative, detailed description of individual and social behavior in a free-living population of thirteen-lined ground squirrels. Behavioral differences in relation to various periods of the annual cycle are also evaluated.
Contributing Partner: UNT Libraries
Induced Bradycardia Effects on Angiogenesis, Growth and Development in Early Development in Chicken Embryos, Gallus Domesticus

Induced Bradycardia Effects on Angiogenesis, Growth and Development in Early Development in Chicken Embryos, Gallus Domesticus

Date: December 2010
Creator: Ruck, Sylvia A.
Description: Cardiac performance, angiogenesis and growth was investigated during early chicken development. Heart rate, and thus arterial pulse pressure and cardiac output, were altered with the bradycardic drug ZD7288. Heart rates at 72 h of development of control embryos and those dosed with chicken Ringer were not different at 171 bpm. Acute and chronic application of ZD7288 caused significant bradycardia. Chronic dosing of Ringer and ZD7288 changed neither eye diameter nor development rate. Chronic dosing of ZD7288 did not significantly alter CAM vessel density close to the embryo (2, 3 and 4 mm) but at farther distances (5 and 6 mm) chronic dosing with both Ringer and ZD7288 decreased vessel density by 13 - 16%. Chronic dosing with ZD7288 also reduced body mass by 20%. Thus, lowered heart rate and cardiac output had little effect on vessel density or developmental stage, but did reduce embryo growth.
Contributing Partner: UNT Libraries