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 Degree Discipline: Biology
Effects of CFT Legumine™ Rotenone on Macroinvertebrates in Four Drainages of Montana and New Mexico

Effects of CFT Legumine™ Rotenone on Macroinvertebrates in Four Drainages of Montana and New Mexico

Date: August 2011
Creator: Skorupski, Joseph A., Jr.
Description: Rotenone is considered essential in the restoration of native fish populations; however, the technique is contentious and criticized, specifically concerning impacts to invertebrates. Knowledge of effects to non-target organisms is important for the management and conservation of fish populations. This thesis has two general objectives: (1) demonstrate the influence CFT Legumine™ rotenone has on benthic macroinvertebrates for restoration projects in Montana and New Mexico and (2) evaluate the immediate response by means of invertebrate drift. Chapters 2 and 4 incorporate results from four different restoration projects that examine benthic macroinvertebrate response. Results indicate treatment effects are minimal for Specimen and Cherry Creek projects in Montana. New Mexico projects, Comanche and Costilla Creek suggest a greater influence. Potassium permanganate used to neutralize rotenone, influenced communities in three of the four projects. Regardless, invertebrates in all four projects recovered one-year after treatment. Chapter 3 examines macroinvertebrate drift during rotenone treatment. Results suggest a delayed response compared to previous literature. Rotenone appears to have the greatest immediate influence on the early life stages of Ephemeroptera and Plecoptera. To reduce impacts of rotenone to invertebrates, managers should apply CFT Legumine and use the minimal dosage and duration to complete the projects goal of removing ...
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The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes

The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes

Date: August 1959
Creator: Sissom, Stanley L.
Description: The role that aquatic actinomycetes play, in the production of tastes and odors in water supplies has been investigated since 1948. The ability of these organisms to produce by-products in lakes and streams, which renders the water unpalatable, is of considerable public health importance. It is desirable that the waterworks industry has as much information as possible concerning the factors that contribute to the growth of these organisms. Since it appears that the aquatic actinomycetes may be isolated from most fresh-water sources, the problem of diversified environments and nutritional requirements offers an excellent field of investigation. The fresh-waters of the world contain variable quantities of electrolytes that may determine in part the biological activity of these organisms. The unsolved questions in this instance are concerned with the electrolytes present and their quantitative effects on the growth and development of these forms.
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Effects of Copper on Immune Responses of Largemouth Bass, Micropterus salmoides

Effects of Copper on Immune Responses of Largemouth Bass, Micropterus salmoides

Date: August 1994
Creator: Connell, Patrice M. (Patrice Michelle)
Description: Copper exposures of 400 μg/L for 5,10 and 15 days resulted in no significant differences in antibody titers of largemouth bass, Micropterus salmoides injected with Aeromonas hydrophila compared to control-injected bass. Twenty days of exposure did significantly increase titers. The control group had significantly lower antibody titers than either control-injected or copper-exposed.
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Effects of Daily Oral Injections of Quercetin on Implanted Colon-25 Tumor Growth in Balb-C Mice

Effects of Daily Oral Injections of Quercetin on Implanted Colon-25 Tumor Growth in Balb-C Mice

Date: May 2000
Creator: Hayashi, Adam
Description: The effects of three oral dosages (0.4 mg, 0.8 mg, and 1.6 mg) of quercetin on Colon-25 tumors implanted in Balb-c mice were studied. The data in this study show that: (1) certain dosages of quercetin in alcohol solutions, reduces the weight, and size of implanted Colon-25 tumors in Balb-c mice, (2) these same dosages of quercetin all produce a profound neutrophilia combined with a significant lymphopenia at day 20 post-implantation, and (3) there was relatively little evidence of histological changes in the quercetin-treated tumor section which would indicate that the action(s) of quercetin is primarily at the subcellular level probably within the nuclei of the tumor cells.
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Effects of External Electric Fields on Light Transmittance in Isolated Crayfish Nerves

Effects of External Electric Fields on Light Transmittance in Isolated Crayfish Nerves

Date: December 1995
Creator: Northcutt, Brian S. W.
Description: Acute effects of a pulsed external electric field (PEEF) at 20 V/cm and a d.c. EEF at 90 V/cm on light transmittance in an isolated compound crayfish nerve was measured. In a third series, the nerve was pre-treated with the Na+ channel blocker tetrodotoxin (TTX). A PEEF produced an irreversible increase in the variation of light transmittance in normal nerves but a reversible increase in TTX treated nerves. This data was statistically insignificant. The d.c. EEFs produced a reversible and statistically significant enhancement of variation in light transmittance in both untreated and TTX-treated nerves. The findings may be due to either (1) an alteration in the ion/fluid flux within the nerve or (2) a physical alteration of protein molecules in the membranes.
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The Effects of Heparin on the Development of Resistance to Antibiotics by Staphylococcus Aureus

The Effects of Heparin on the Development of Resistance to Antibiotics by Staphylococcus Aureus

Date: August 1959
Creator: Blanton, William George
Description: Since heparin combines with some antibiotics to decrease the toxicity of the antibiotic to the patient, the purpose of this investigation is to determine whether it has any effect upon the development of resistance to antibiotics by Staphylococcus aureus.
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The Effects of Hexadecanol on the Microbiota of Lake Hefner

The Effects of Hexadecanol on the Microbiota of Lake Hefner

Date: May 1968
Creator: Dickson, Kenneth L.
Description: It seemed desirable to investigate more fully the effect of hexadecanol on the microbial population of a reservoir. It was the purpose of this investigation to determine the effect of hexadecanol on the micro-biota of Lake Hefner, to ascertain which organisms were stimulated by hexadecanol both in the laboratory and the reservoir, and to investigate the degradation of hexadecanol by microorganisms selected from Lake Hefner.
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Effects of High Altitude Exposure on Capillary Permeability

Effects of High Altitude Exposure on Capillary Permeability

Date: December 1971
Creator: Reaves, Troy Albert
Description: Observations of decreases in plasma volume, shifts in plasma and lymph protein concentrations, and increases in capillary permeability at high altitude have been reported in the literature by several investigators. This investigation was begun in an attempt to elucidate the possible significance of these phenomena in future space exploration, and because of the lack of knowledge concerning the underlying mechanisms. The purpose of this investigation was to determine the effects of exposure to hypobaric pressures on the capillary permeability to the normal plasma and lymph proteins.
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The Effects of Hypothermia on the Release of Cardiac Enzymes

The Effects of Hypothermia on the Release of Cardiac Enzymes

Date: August 1977
Creator: Strawn, William B.
Description: The myocardium is known to release CPK, LDH1 , and GOT in response to ischemia as a result of myocardial infarction. This study was designed to induce the release of cardiac enzymes without adversely effecting the myocardium by perfusion hypothermia, thereby suggesting that these enzymes are not as specific in the diagnosis of myocardial infarction as once thought. Hypothermia was by in vivo perfusion of the left anterior descending coronary artery. Enzyme activity was measured from sera samples spectrophotometrically and electrophoretically. Significant CPK and LDH1 increases were observed in animals perfused between 25 and 19 C. These results indicate that, while heart function remained unchanged, an alteration occurred in the membrane integrity of the myocardial cells.
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The Effects of Inbreeding on Fitness Traits in the Critically Endangered Attwater’s Prairie-chicken

The Effects of Inbreeding on Fitness Traits in the Critically Endangered Attwater’s Prairie-chicken

Date: August 2014
Creator: The effects of inbreeding on fitness traits in the critically endangered Attwater’s prairie-chicken.
Description: The goals of captive breeding programs for endangered species include preserving genetic diversity and avoiding inbreeding. Typically this is accomplished by minimizing population mean kinship; however, this approach becomes less effective when errors in the pedigree exist and may result in inbreeding depression, or reduced survival. Here, both pedigree- and DNA-based methods were used to assess inbreeding depression in the critically endangered Attwater’s prairie-chicken (Tympanuchus cupido attwateri). Less variation in the pedigree-based inbreeding coefficients and parental relatedness values were observed compared to DNA-based measures suggesting that errors exist in the pedigree. Further, chicks identified with high parental DNA-based relatedness exhibited decreased survival at both 14- and 50-days post-hatch. A similar pattern was observed in later life stages (> 50 days post-hatch) with birds released to the wild; however, the pattern varied depending on the time post-release. While DNA-based inbreeding coefficient was positively correlated with mortality to one month post-release, an opposite pattern was observed at nine months suggesting purging of deleterious alleles. I also investigated whether immunocompetence, or the ability to produce a normal immune response, was correlated with survival; however, no significant correlation was observed suggesting that inbreeding was a more important factor influencing survival. Pairing individuals for breeding ...
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The Effects of Media Constituents Upon the Growth and Pigment Production of Micrococcus Flavus, Micrococcus Roseus, Micrococcus Subcitreus, and Sarcina Citrea

The Effects of Media Constituents Upon the Growth and Pigment Production of Micrococcus Flavus, Micrococcus Roseus, Micrococcus Subcitreus, and Sarcina Citrea

Date: 1947
Creator: Martin, Joseph Hearn
Description: This investigation has for its aim the explanation of growth and pigment production of Micrococcus flavus, Micrococcus roseus, Micrococcus subvitreus, and Sarcina citrea by the addition of various nutrient test materials to a standard culture medium.
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Effects of Methanol, Atrazine, and Copper on the Ultrastructure of Pseudokirchneriella Subcapitata (Selenastrum Capricornutum).

Effects of Methanol, Atrazine, and Copper on the Ultrastructure of Pseudokirchneriella Subcapitata (Selenastrum Capricornutum).

Date: May 2004
Creator: Garrett, David C.
Description: The toxicity of methanol, atrazine, and copper to Pseudokirchneriella subcapitata (Korshikov) Hindák historically referred to as Selenastrum capricornutum Printz were determined following 96 hrs growth in a modified Goram's growth media. Methanol and atrazine inhibited fluorescence readings in the cultures by 50% (IC50) at concentrations of 2% and 82 µg/l respectively. These toxicity values compared favorably to other published reports. The IC50 for copper was 160 µg/l which is substantially higher than reported values. This is understandable because of the high chelating capacity of Goram's media. The use of stereologically derived relative volume in the chloroplasts, mitochondria, lipid bodies, phosphate bodies, and nucleus was investigated to determine if it could be used as a sensitive endpoint in toxicity tests. The volume fractions for the chloroplasts and mitochondria were normally distributed in control cells while the nuclei, phosphate bodies, and lipid bodies were not. The chloroplasts were the most dominate organelle occupying a mean relative volume of 46% and mitochondria occupied a mean relative volume of 3%. The nucleus and phosphate bodies occupied a median relative volume of 7% and 2% respectively. The lipid bodies were rare in section profile and no meaningful median relative volume could be calculated. Up to ...
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Effects of n-Decenylsuccinic Acid (n-DSA) and It's Monomethyl Ester (m-MDSA) on Water and Ion Flux in Isolated Roots

Effects of n-Decenylsuccinic Acid (n-DSA) and It's Monomethyl Ester (m-MDSA) on Water and Ion Flux in Isolated Roots

Date: January 1969
Creator: Teng, Yao-sheng
Description: The purpose of this study was (1) to measure the changes in water flux in isolated roots exposed to n-DSA and its mono-methly ester (m-MDSA) using a photometric technique in the absence of an externally applied suction and (2) to measure the flux of Na and K in similarly treated roots using a flame photometric method.
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The Effects of Organic Surface Amendments on Soil Nutrients and Initial Tree Establishment

The Effects of Organic Surface Amendments on Soil Nutrients and Initial Tree Establishment

Date: May 1999
Creator: Thuesen, Kevin (Kevin Andrew)
Description: This study examined the effects of replicating woodland soil surface horizonation on the nutrient status of underlying soils and the initial establishment and growth of trees. A total of 283 container grown trees were planted in a bufferzone around a future landfill site. Control amendments consisted of an 8 cm layer (0.5 m3) of wood chips applied in a circular area of 4.6 m2 around the trees' planting pit. For the treatment, a 2.5 cm layer of composted biosolids (0.15 m3 or 80 Mg/ha) was applied in a circular area of 4.6 m2 around the trees' planting pit followed by an 8 cm layer (0.5 m3) of wood chips. The results indicate that the replication of woodland soil surface attributes using composted biosolids can significantly improve the nutrient status of underlying soil. Some significant effects were seen under control conditions, too. However, the effects on tree establishment and growth parameters were, for the most part, not statistically significant.
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Effects of Peripheral Nerve Injury on the Cells of the Dorsal Root Ganglion: a Role for Primary Cilia

Effects of Peripheral Nerve Injury on the Cells of the Dorsal Root Ganglion: a Role for Primary Cilia

Date: December 2012
Creator: Smith, Sarah K.
Description: Primary cilia are ubiquitous sensory organelles found on most cell types including cells of the dorsal root ganglia (DRG). The DRG are groups of peripheral neurons that relay sensory information from the periphery to the CNS. Other cell types in the DRG include a type of glial cell, the satellite glial cells (SGCs). The SGCs surround the DRG neurons and, with the neurons, form functional sensory units. Currently are no reports describing the numbers of DRG cells that have cilia. We found that 26% of the SGCs had primary cilia. The incidence of cilia on neurons varied with neuron size, a property that roughly correlates with physiological characteristics. We found that 29% of the small, 16% of the medium and 5% of the large neurons had primary cilia. Primary cilia have been shown to have a role in cell proliferation in a variety of cell types. In some of the cells the cilia mediate the proliferative effects of Sonic hedgehog (Shh). In the CNS, Shh signaling through primary cilia affects proliferation during development as well as following injury, but no studies have looked at this function in the PNS. The SGCs and neurons of the DRG undergo complex changes following ...
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Effects of pH and Substrate on Growth of Escherichia Coli and Pseudomonas Aeruginosa in Mixed Continuous Culture

Effects of pH and Substrate on Growth of Escherichia Coli and Pseudomonas Aeruginosa in Mixed Continuous Culture

Date: January 1964
Creator: Cooper, Billy Howard
Description: The express purpose for doing this project was to develop methods for the continuous culture of E. coli and P. aeruginosa as a mixed population, and to apply these methods in studying the effects of pH and substrate upon the growth of these two organisms in mixed continuous culture.
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Effects of Ribavirin on Normal Rat Kidney Cells and Chicken Embryo Fibroblasts Infected with Rous Sarcoma Virus

Effects of Ribavirin on Normal Rat Kidney Cells and Chicken Embryo Fibroblasts Infected with Rous Sarcoma Virus

Date: April 1979
Creator: Jenkins, Frank J.
Description: Ribavirin, a synthetic nucleoside, was found to inhibit the replication of Rous sarcoma viruses (RSV) and subsequent cell transformation in chick embryo fibroblasts (CEF). It also blocked the transformation of normal rat kidney (NRK) cells infected with temperature-sensitive mutants of RSV. The action of Ribavirin was found to be reversible as removal of the drug from the NRK cells reversed the effects on cell transformation. Ribavirin appears to have a static effect on cell growth of both NRK and CEF cells. In addition, guanosine, xanthosine and inosine altered the effect of Ribavirin on cell growth.
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The Effects of Selected Algicides and Some Coordination Complexes upon the Apparent Photosynthesis of Chlorella Pyrenoidosa

The Effects of Selected Algicides and Some Coordination Complexes upon the Apparent Photosynthesis of Chlorella Pyrenoidosa

Date: June 1965
Creator: Phelps, Robert G.
Description: Many experiments have been performed with the Warburg apparatus, or variations of this manometric technique, since Warburg's experiments (52, 53) where the effects of cyanides upon dark reactions and of urethanes upon light reactions of photosynthesis were demonstrated. The same basic techniques were utilized in this research in attempting to determine the effects of some coordination complexes upon the apparent photosynthetic rate of Chlorella pyrenoidosa. A second goal of the present paper was to investigate the potential of the Warburg apparatus as a tool for screening algicidal compounds.
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Effects of Selected Phytohormones on the Growth and Morphology of Escherichia Coli

Effects of Selected Phytohormones on the Growth and Morphology of Escherichia Coli

Date: January 1968
Creator: Little, Lynn Mallory
Description: The present investigation was undertaken as a preliminary study to learn the response of Escherichia coli cells grown under identical experimental conditions to various concentrations of indoleacetic acid, gibberellic acid, and kinetin alone, and in combination with one another.
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Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis

Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis

Date: December 2014
Creator: Carty, Dennis R.
Description: Sertraline is a selective serotonin reuptake inhibitor (SSRI) that is widely used for the treatment of depression and anxiety. Due to the abundant therapeutic use of sertraline, low levels have been detected in municipal wastewater effluents suggesting that aquatic organisms may be exposed. The purpose of this study was to evaluate the steroidogenic effects of sertraline on larval (FHM) and adult female fathead minnows (FFHM), Pimephales promelas. Larval FHM were exposed to 0.1, 1, and 10 µg/L sertraline for 28 days and analyzed via RT-qPCR for differential expression of 11β-Hydroxysteroid dehydrogenase (11β-HSD), 20β-Hydroxysteroid dehydrogenase (20β-HSD), aromatase (CYP19), and nuclear thyroid receptor alpha (TRα). FFHM were exposed to 3 or 10 µg/L sertraline for 7 days with the brain and ovary excised at exposure termination. Juvenile FHM exposed to 0.1 μg/L sertraline had a significant upregulation of both 20β-HSD and TRα. FFHM exposed to 10 µg/L sertraline had a significant upregulation of 11β-HSD expression in brain tissue, while no steroidogenic changes were observed in the FFHM ovary. Similarly, in FFHM brain tissue, CYP19 and 20β-HSD expression levels were significantly higher in fish exposed to 10µg/L sertraline compared to control. The significance of these findings with respect to survival, growth and reproduction ...
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The Effects of Simulated Altitude on the Intestinal Flora of Guinea Pigs

The Effects of Simulated Altitude on the Intestinal Flora of Guinea Pigs

Date: May 1969
Creator: Funderburk, Noel R.
Description: The purpose of this paper is to report the results of studies on the aerobic, mesophilic intestinal flora of guinea pigs subjected to conditions similar to those encountered by man in spacecraft.
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The Effects of the Soil Conditioner, Superbio, Upon the Cellulose Decomposing Bacteria and the Crop Yield of a Soil

The Effects of the Soil Conditioner, Superbio, Upon the Cellulose Decomposing Bacteria and the Crop Yield of a Soil

Date: August 1968
Creator: Gunn, Bruce Alan
Description: The purpose of this investigation was to determine if a commercial soil conditioner, Superbio, can improve crop yield, and if the "advertised" soil improvement might be due to an increase in the activity and numbers of aerobic cellulose decomposing bacteria following treatment.
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Effects of Triclosan, Triclocarban, and Caffeine Exposure on the Development of Amphibian Larvae.

Effects of Triclosan, Triclocarban, and Caffeine Exposure on the Development of Amphibian Larvae.

Date: August 2009
Creator: Palenske, Nicole Marie
Description: Triclosan and triclocarban are antimicrobials found in numerous consumer products, while caffeine is the most commonly consumed stimulant by humans. This study was undertaken to determine the effects of triclosan, triclocarban, and caffeine on the development and physiology of amphibian larvae. LC50 values of triclosan and triclocarban were determined after 96 hours for three North American larval species: Acris crepitans blanchardii, Bufo woodhousii woodhousii, Rana sphenocephala, and for a common amphibian developmental model: Xenopus laevis. Amphibian larvae were most sensitive to triclosan and triclocarban exposure during early development based upon 96-hour LC50 values. Heart rates for X. laevis and North American larvae exposed to triclosan were variable throughout development. However, significantly lower heart rates were observed in all larvae exposed to triclocarban. Metabolic rates of X. laevis and R. sphenocephala larvae exposed to triclosan were significantly affected in larvae exposed to ½ LC50 and the LC50 concentration. Metabolic rates of X. laevis larvae exposed to triclocarban were significantly affected by exposure to ½ LC50 concentrations in three of four stages investigated. No significant differences were observed in North American larvae exposed to triclocarban. Tissue uptake, lipid uptake, tissue bioconcentration factor (BCF) and lipid BCF of triclosan and triclocarban were investigated ...
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Effects of Turbidity on Gilling Rates and Oxygen Consumption on Green Sunfish, Lepomis cyanellus

Effects of Turbidity on Gilling Rates and Oxygen Consumption on Green Sunfish, Lepomis cyanellus

Date: May 1974
Creator: Horkel, John Duane
Description: Laboratory studies conducted at 5, 15, 25, and 35 C measured changes in gilling rates and oxygen consumption of green sunfish in response to exposure to bentonite clay suspensions. The tests indicate that gilling rates are not affected by bentonite clay suspensions below 2125 FTU at 5 C, 1012 FTU at 15 C, and 898 FTU at 25 C. At turbidity levels exceeding 1012 FTU at 15 C and 898 at 25 C, gilling rates increased 50-70%. Tests were inconclusive at 35 C. Oxygen consumption rates were found to be unaffected by turbid suspensions below 3500 FTU at all four temperatures. Evidence suggests that increased gilling rates under highly turbid conditions are a means of compensating for reduced respiratory efficiency and a strategy for maintaining a constant oxygen uptake. Evidence indicates that the cost of increased gilling rates is probably met by a reduction in activity.
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