Isolation and Characterization of Two Enzyme Proteins Catalyzing Oxido-Reduction at C-9 and C-15 of Prostaglandins from Swine Kidney
Description:
Two swine kidney proteins (PI 4.8 and 5.8) both possessing 9-prostaglandin ketoreductase (9-PGKR) and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) activities were purified to homogeneity. Purification increased specific activities in parallel. Molecular weight, subunit size, amino acid composition, coenzyme and substrate specificity and antigenicity of both proteins were similar. Gel filtration and SDS-polyacrylamide gel electrophoresis molecular weights of 29,500 and 29,000, respectively, s…
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Date:
December 1980
Creator:
Chang, David Guey-Bin