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Actinoplanes Philippinensis: Effect of Carbon Sources on Zoospore Production: Actinomycetes are able to utilize a great variety of carbohydrates, like sugar. The particular kind of sugar and its concentration has decisive effect on the growth of microorganisms. The proper nutritional media aids also in the production of spores. Based on this generalization, that the growth and sporulation of microorganisms are greatly influenced by the nature and the concentration of carbohydrates, an attempt has been made to study Actinoplanes philippinensis with respect to this influence.
Acute Effects of the Antibiotic Streptomycin on Neural Network Activity and Pharmacological Responses: The purpose of this study is to find out that if antibiotic streptomycin decreases neuronal network activity or affects the pharmacological responses. The experiments in this study were conducted via MEA (multi-electrode array) technology which records neuronal activity from devices that have multiple small electrodes, serve as neural interfaces connecting neurons to electronic circuitry. The result of this study shows that streptomycin lowered the spike production of neuronal network, and also, sensitization was seen when neuronal network pre-exposed to streptomycin.
The Adaptability and Present Status of Andropogon Ischaemum L. in Denton County, Texas: This investigation has been made with reference to the adaptability of Andropogon ischaemum, King Ranch bluestem, to various soils, various slopes, and various erosion conditions in Denton County.
Adenylate Energy Charge Determinations of Soil Bacteria Grown in Soil Extract Medium: The adenylate energy charge values of twenty bacteria isolated from soil and cultured in a medium consisting of soil and distilled water were determined by the luciferin-luciferase bioluminescense method. The purpose of this study was to examine the growth and energy charge values of these organisms in soil extract medium, and to determine what effect the addition of glucose has on their energy charge values. Three of the organisms employed in this study showed energy charge values similar to those reported for bacteria grown in enriched media. The remainder of the isolates demonstrated low energy charge values, and scant growth in the soil medium.
Advanced Molecular and Microbial Techniques: a Complete Laboratory Notebook: The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summer semester at the University of North Texas. This class is a team taught effort by Professors O'Donovan and Kunz. The course is constructed as an intensive practice exercise to teach the student about gene mutations, biosynthetic pathways, preparation and analysis of plasmid DNA, and many other topics included in the notebook.
Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course: Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr G.A.O. Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiment, essays,. preparation and analysis of plasmid DNA and various other topics. Part 2 covers the experiments conducted by Dr. Daniel Kunz and includes various topics like media preparation, phenotyping strains, conjugative transfer of plasmids, SDS-PAGE, induction and measurement of enzyme and transposon mutagenesis
The Age and Rate of Growth of the Black Crappie, Pomoxis Nigro-Maculatus (Le Sueur), and the White Crappie, Pomoxis Annularis Rafinesque, in the Koon Kreek Klub Lakes, Texas: This study is another phase of the overall research program designed to furnish basic information for the management of East Texas lakes and has as its specific purpose the determination of the age and growth characteristics of two valuable species on these waters. The species to be considered are the black crappie, Pomoxis nigro-maculatus (Le Sueur) and the white crappie, Pomoxis annularis Rafinesque. Both species are highly regarded as food fish and have for many years offered recreational outlets for Texas fishermen.
The Age, Growth, and Food Habits of the Lake Dallas White Bass, Lepibema Chrysops (Rafinesque): This study was made to secure certain new information concerning the food habits and growth of the white bass, Lepibema chrysops (Rafinesque), which is quite generally considered a good game and good fish.
Age, Growth, and Food Habits of the Largemouthed Black Bass (Micropterus Salmoides) and the Spotted Bass (Micropterus Puctulatus SSP) in North and East Texas Lakes: This study is based on the examinations and analyses of 388 largemouthed black bass (Micropterus salmoides) and 118 spotted black bass (Micropterus puctulatus ssp.) which were collected over a period of three years from 9 lakes in the North-Central and East Texas areas. The results should show the following: (1) the average age of the black bass in each of the nine reservoirs in Texas; (2) the growth rate of the bass in these reservoirs; (3) the feeding habits of the bass in all reservoirs studied; (4) a comparison of the growth rates of the largemouthed and spotted basses; and (5) a comparison of the growth rates of bass from Texas and Oklahoma.
Air Breathing Fish: Development of Air Breathing in Bristlenose Plecos (Ancistrus cirrhosus): The bristlenose pleco (Ancistrus cirrhosus) is a species of armored catfish in the Loricariidae family that breathes air facultatively when the aquatic environment becomes hypoxic. The bristlenose pleco uses its highly vascularized stomach as an air breathing organ. The two main goals of this developmental study were to determine the size of onset of air breathing and to determine the frequency of air breathing behavior in bristlenose plecos from juveniles to adults. Developing juveniles reach functional maturity within four to six months of hatching and grow to an adult size of eight to ten cm in length. To examine the developmental timing for the onset of air breathing, we tested different sized juveniles beginning at one cm up until 8 cm in length. The developmental timing for the onset of air breathing was measured by exposing each fish to a slowly decreasing aquatic oxygen content from 100% air saturation down to 8% air saturation. Fish were first able to breathe air at just over 2 cm and 1 gram in mass. There was a weak negative correlation between fish length and % air saturation at which air breathing began. When exposed to 15% air saturation, frequency of air breathing was negatively correlated with fish length. Armored catfish are becoming an invasive species in the southern US, outcompeting local fauna potentially because of this adaptation. This research provides important insight into the development of the air breathing adaptation that may allow these fish to outcompete the others.
Altered Leukemogenic Activity by Thyrotropic Treatment of Leukemic Mouse Thymus in Vitro: This investigation was planned to observe if the leukemic thymic tissue in vitro would inactivate TSH and to study the effect that TSH would have on the leukemogenic properties of these cells when transplanted into a high-leukemia strain of mice.
Anaerobic Organisms in Acute and Chronic Pulmonary Diseases: This study concerns a determination as to whether anaerobic organisms are involved in pulmonary diseases, particularly those of the chronic type.
An Analysis and Evaluation of the Elementary Science Curricula in the State Universities and Leading Teachers Colleges in the United States: The purpose of this study is to determine the status of elementary science curricula in the different state universities and the leading teachers colleges in the United States.
An Analysis of Distribution Patterns of Amphibians and Reptiles in Texas: The purpose of this investigation was to analyze the distribution of amphibians and reptiles in Texas by means of the methods of Webb and Hagmeier and Stults. An additional graphical analysis was made, including range and range limits which provides a cross-section of faunal change along selected base lines across the state.
An Analysis of the Seasonal Food Habits of Two Species of Texas Centrarchids: This thesis explores the feeding habits of sunfish and bluegill in Texas lakes. Data gathered for this study is aimed to aid conservation of these pan fish by making a contribution toward a greater life history understanding of each species.
Anatomical and Morphological Responses of Cardiospermum Halicacabum L. (Balloon Vine), to Four Levels of Water Availability: C. halicacabum (Sapindaceae) is an invasive plant that is considered a nuisance species in Texas riparian environments. Little is known of the tolerance of C. halicacabum to flooding and drought; however, this information may provide insight into the characteristics that contribute to C. halicacabum purported invasiveness. C. halicacabum seedlings (n = 92) were exposed to one of four levels of water availability (flooded, saturated, intermediate and dry) over six weeks under greenhouse conditions. Plant performance was affected by water availability; however, there was no effect on survivorship. Flooded and saturated plants exhibited morphological adaptations; producing adventitious roots, hypertrophy, and aerenchyma tissue. Morphological measures, anatomical responses, and patterns of biomass allocation all indicate that C. halicacabum is able to survive periodic inundation, perform in saturation, and establish and thrive on the drier end of a moisture gradient.
Anatomy and Physiology Syllabus for Community Colleges: This syllabus includes both lecture notes and laboratory activities for a two-semester anatomy and physiology community college course. The syllabus is based on a 16-week semester that is comprised of a three-hour lecture section and a one-hour laboratory class each week. Both the lecture course and laboratory are necessary to fulfill the requirement for anatomy and physiology. Laboratory activities coincide with lectures to enhance understanding of each topic by providing visual and hands-on experiments for the concepts learned in the lecture. Laboratory quizzes will be given each week to help students maintain a working knowledge of the material learned in the laboratory. This course is appropriate for the typical anatomy and physiology student and should benefit both students who plan to major in biology and also those who are non-biology majors. Because subject matter in anatomy and physiology is quite difficult, the importance of attendance and good study skills is stressed.
Annual Lipid Cycles in the Lizard Cnemidophorus Tigris: Annual lipid cycles were determined for adult male and female Cnemidophorus tigris collected near El Paso, Texas during 1970-1971.
Antibacterial Effect of the Oleoresins of One Hundred Common Texas Plants upon Twenty-Five Gram-Negative Mirco-Organisms: This investigation deals with the study of the possible antibiotic effect of oleoresins of one hundred common Texas plants upon twenty-five gram-negative bacterial organisms.
Antibiocity of the Oleoresins of One Hundred Texas Spermatophytes upon Twenty Gram-Positive Bacterial Organisms: This investigation is concerned with the testing, in vitro, of oleoresins from one hundred higher plants common to North and South Central Texas. The plants used were selected in order to obtain a representative collection which might be of value in future research.
The Antibiotic Activities of Some Members of the Cactaceae Family: This problem has been concerned with, first, the collection of sixteen species of plants belonging to Cactaceae family; second, the drying of these and the extraction of the oleoresins thereof; third, the determination of the extent to which these substances inhibit the growth of ten gram-positive and ten gram-negative bacterial organisms; and fourth, a determination of the possible utilization of these extracts a prophylactic or chemotherapeutic agents.
The Antibiotic Effect of Pigmented Actinomycetes: This thesis attempts, first, to correlate pigmentation of actinomycetes with the degree of antibiocity; second, to establish the most favorable means of producing a rich luxuriant pigmentation in the individual organism studied by enriching favorable media with specific types of proteins and amino acids.
The Antibiotic Properties of the Oleoresins of Twenty-Five Common Garden Vegetables: The purpose of this problem is to determine the presence and extent of antibiotic materials as found in the oleoresins of a selected group of garden vegetables. The problem has consisted of, first, the collection and preparation of specimens of twenty-five commonly used garden vegetables; second, the extraction of the oleoresins from these; third, the determination of the inhibitory and other effects of these oleoresins against several strains of selected gram-positive and gram-negative bacteria; and fourth, the evaluation of the potentialities of these oleoresins with regard to their future use as medicinal prophylactics and therapeutics.
Antibiotic Sensitivity of Bacteria Isolated from City Water Distribution Systems: This thesis studies antibiotic sensitivity of bacteria isolated from city water distribution systems.
Antibody Adsorption Used in Identification of Similar Streptomyces Species: This investigation involved the production of specific antisera against known International Streptomyces project strains of Streptomyces.
The Application of Chlorine Dioxide to Tastes and Odors in Water Supplies: This problem was undertaken to determine the potentialities of chlorine dioxide as an algaecide and as an agent for the removal of tastes and odors from fresh water.
Application of Fluorescent Antibody Methods for the Enumeration and Identification of Bacillus Cereus: This particular work is proposed as a test of the expedience of using the fluorescent-antibody technique as a method for enumeration and identification of certain strains of B. cereus that have been found to be effective in preventing taste and odor in water supplies resulting from certain Actinomycete blooms.
Applications of Remote Sensing and GIS to Modeling Fire for Vegetative Restoration in Northern Arizona: An accurate fire model is a useful tool in predicting the behavior of a prescribed fire. Simulation of fire requires an extensive amount of data and can be accomplished best using GIS applications. This paper demonstrates integrative procedures of using of ArcGIS™, ERDAS Imagine™, GPS, and FARSITE© to predict prescribed fire behavior on the Kaibab-Paiute Reservation. ArcGIS was used to create a database incorporating all variables into a common spatial reference system and format for the FARSITE model. ArcGIS Spatial Analyst was then used to select optimal burn sites for simulation. Our predictions will be implemented in future interagency efforts towards vegetative restoration on the reservation.
Aquatic Vegetation Nutrient Budgets and Sedimentation in a Southwestern Reservoir: During four growing seasons, aquatic vascular plant production and distribution were studied in Pat Mayse Lake, Texas, a 2425 hectare oligo-mesotrophic reservoir. The dominant macrophyte population was Myriophyllum spicatum L. Growth rates and regrowth rates of mechanically harvested Myriophyllum beds were found to be dissimilar. Based on estimates of watermilfoil nutrient content, there were insufficient nutrients in the entire population to alter the trophic status of this reservoir should all of the nutrients be instantaneously released. Sediments were the primary nutrient (nitrogen and phosphorus) sink. Bank erosion and solids transport from the watershed appear to contribute most of the sediments and a lake-wide mean sedimentation rate of 2.5 cm/year was estimated from sediment trap and core sample data.
Aryl Hydrocarbon Hydroxylase and Sixteen Alpha Hydroxylase in Cultured Human Lymphocytes: Cultured human lymphocytes may be assayed for aryl hydrocarbon hydroxylase (AHH) in whole cell preparations. The optimum assay conditions are pH 8.5, and 1.5 mM Mg++. The reaction is linear with time and cell number, and is inhibited by CO. Estradiol may inhibit induction of AHH by 3-methylcholanthrene, but is a poor competitor for the enzyme. A Caucasian population was assayed for AHH activity. The distribution was lognormal; no difference was found in cultured cells from males and females or smokers and nonsmokers. Cells from relatives of lung cancer patients showed higher activity. An American Indian population showed no difference from the Caucasian population in enzyme level. No linkage was found between AHH and 16a-hydroxylase.
Aspartate Transcarbamoylase of Aeromonas Hydrophila: This study focused on the enzyme, aspartate transcarbamoylase (ATCase) from A. hydrophila, a Gram-negative bacterium found in fresh water. The molecular mass of the ATCase holoenzyme from A. hydrophila is 310 kDa. The enzyme is likely composed of 6 catalytic polypeptides of 34 kDa each and 6 regulatory polypeptides of 17 kDa each. The velocity-substrate curve for A. hydrophila ATCase is sigmoidal for both aspartate and carbamoylphosphate. The Km for aspartate was the highest to date for an enteric bacterium at 97.18 mM. The Km for carbamoylphosphate was 1.18 mM. When heated to 60 ºC, the specific activity of the enzyme dropped by more than 50 %. When heated to 100 ºC, the enzyme showed no activity. The enzyme's activity was inhibited by ATP, CTP or UTP.
An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa: The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the isotopicallylabelled, liquid-liquid partitioning assay is reliable, sensitive and adaptable to rapid assay conditions. It was also determined that different strains of Pseudomonas aeruginosa produce varying amounts of lipase. Partial purification of supernatant by gel filtration produced two protein peaks showing enzymatic activity.
Assessing Effects of an Environmental Education Field Science Program Fostering Responsibility at an Urban Middle School: The study investigated the ability of an extracurricular program to influence environmental responsibility of sixth and seventh graders. The Children's Environmental Attitude and Knowledge Survey (CHEAKS) was evaluated for appropriateness in assessing the worth of this particular environmental education strategy emphasizing water quality fieldwork and technology. CHEAKS is designed with psychometric reliability and validity that may be used in comparing disparate programs. Wilcoxon two sample tests were used to analyze data gathered from two student groups; one participated in an "Enviro-Mentals Club"; the other received no treatment. Analysis showed no significant change in environmental attitudes between groups, but did show significance (p <= 0.05) in environmental knowledge growth. Therefore, the investigated program had marginal success in influencing environmental responsibility.
Assessment of Changes in Aquatic Macrophyte Occurrence Following Introduction of Triploid Grass Carp in a North Texas Reservoir: The objectives of this project were to measure changes in frequency of occurrence of submerged macrophytes over the first two growing seasons following stocking with triploid grass carp at two fish per acre and to measure differences in macrophyte biomass between areas excluded from herbivory and adjacent control sites after 16 months following establishment of exclosures. The project also seeks to measure concentrations of fluridone following the herbicide treatment, and to compare two methods of aquatic vegetation sampling.
Assimilation of Inorganic Nitrogen by Aquatic Actinomycetes: It was the purpose of this investigation to present laboratory data concerning the assimilation of inorganic nitrogen by the aquatic actinomycetes. The strains of aquatic actinomycetes under consideration represented a cross section of those currently under culture at North Texas State University.
Assimilation of Organic Carbon by Aquatic Actinomycetes: There were two purposes of this investigation: the first was to develop a method for studying spore germination, hyphal growth and mycelial development of the aquatic actinomycetes under the microscope so that the life cycle of this organism could be continuously followed. The second purpose was to determine the rate of carbon utilization from various types of nutritional sources, and to correlate this with the colony development and spore formation.
Attenuation of Escherichia Coli Aspartate Transcarbamoylase Expressed in Pseudomonas Aeruginosa Mutant and Wild Type Strains: No apparent repression of pyr gene expression in Pseudomonas aeruginosa is observed upon addition of exogenous pyrimidines to the growth medium. Upon introduction of the subcloned Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) into a P. aeruginosa pyrB mutant strain, repression was observed in response to exogenously fed pyrimidine compounds. The results proved that it is possible to bring about changes in pyrimidine nucleotide pool levels and changes in transcriptional regulation of gene expression as a result. Thus, the lack of regulatory control in P. aeruginosa pyr gene expression is not due to an inability to take up and incorporate pyrimidine compounds into metabolic pools, or to an inability of the RNA polymerase to respond to regulatory sequences in the DNA but is probably due to a lack of specific regulatory signals in the promoter of the genes themselves.
The Autecology of Celtis Laevigata in Flood Plain Forests of Denton County, Texas: This thesis describes the present nature of one facet of some of the flood plain forest stands in Denton County, Texas. The specific purpose was to demonstrate the presence or absence of difference between the Celtis laevigata (commonly known as the hackberry, southern hackberry or sugarberry) populations in stands on Denton Creek and Elm Fork of the Trinity River.
Autoimmunity of Periodontitis: The purpose of this investigation is to determine if auto-antibodies are demonstrable in inflammatory periodontal disease using methods other than those of Novotny.
Autonomic Nerve Activity and Cardiovascular Function in the Chicken Embryo (Gallus gallus): The goal of this study was to build on the historic use of the avian model of development and also to further the knowledge of autonomic nervous system (ANS) regulation of cardiovascular function in vertebrates. Vasoactive drugs sodium nitroprusside, a vasodilator and phenylephrine, a vasoconstrictor were used to study the correlation of cardiovascular function relationship with nerve activity, both sympathetic and parasympathetic (vagal). Additionally, ANG II was used to assess its effects on vagal inhibition. The present study shows that pharmacologically-induced hypertension is associated with a fall in mSNA, indicating that the capacity for sympathetic autonomic cardiovascular regulation is established by late incubation however, late-stage embryonic chickens did not show a significant increase in mSNA during hypotension. The hypotensive response of the embryo was not accompanied by the expected inhibition of vagal discharge; however a slight but insignificant reduction in vagal discharge was noted. When vagal efferent output was isolated, a significant drop in vagal efferent activity was noted in response to hypotension. The present study showed late-stage embryonic chickens lack a vagal response to hypertension in both efferent and sensory limbs. In this study, vagal discharge was reduced from baseline levels in response to Ang II. Collectively, the present study indicates that the lack of a decreased heart rate, in response to increases in Pm caused by Ang II, is due to a central inhibitory action of Ang II on the vagus. Data from the present study suggests that although autonomic interaction with the cardiovascular system in present in late-stage chicken embryos, it is still underdeveloped and possesses a limited capacity.
Bacterial Antibiotic Properties of the Oleoresins of Thirty Summer Flowering Spermatophytes: The purpose of this investigation is to add to the present day knowledge concerning the presence of antibiotics in additional members of the spermatophyte group of plants.
Bacterial Challenge in Lumbricus Terrestris: A Terrestrial Invertebrate Immunotoxicity Model.: A bacterial challenge assay was developed utilizing the earthworm, Lumbricus terrestris, in order to assess potential immunotoxic effects from exposure to specific polychlorinated biphenyl congeners. Earthworms were inoculated with Aeromonous hydrophila, establishing a 10-day LD50. In vitro assays for effects of PCBs on phagocytosis agreed with mammalian studies, demonstrating potent suppression of phagocytosis by the non-coplanar PCB congener 138 and no suppression by the coplanar congener 126. However, when the effects of the two PCB congeners were evaluated for suppression of resistance to a whole animal infection challenge assay, coplanar PCB 126 decreased the ability of L. terrestris to withstand infection while non-coplanar PCB 138 did not.
A Bacterial Spectrum of Antibiotic "S": This thesis is an investigation of Antibiotic "S" with the purpose of determining which organisms it has a marked effect on, the amount required to cause inhibition, its action in blood, and its therapeutic effect and toxicity in laboratory animals.
Bacterial Survey of Representative Denton County Wells with Special Reference to Sanitation: This thesis aimed to study the seventy-two representative wells of Denton County and to determine the possibilities of infection with typhoid organism. The sanitary survey and the test for Colon-Aerogenes bacteria show that 55% of the 72 wells studied are unsanitary, 27% are sanitary, and 18% are doubtful.
Bacterial Survey of Representative Wells of Canyon, Texas, with Special Emphasis on Sanitation: The problem of this thesis consists of a bacterial analysis of twenty-five representative wells within a radius of thirty miles of Canyon, Texas. An attempt has been made to determine the possible presence of the typhoid organism in these wells.
Bacterial Survey of the Sources of Drinking Water of Trinidad, Texas, with Special Reference to Sanitation: A bacterial analysis of the water from thirty-six sources of consumption by the white population of Trinidad, Henderson County, Texas, was made to determine the potability of each of these in regard to infection from typhoid or related organisms.
Bacterial Utilization of Volatile Substances Produced by Streptomyces Lavendulae: The purpose of this investigation is to attempt to learn something of the biochemical ecology of volatile substances produced by actinomycetes.
Bacteriological Studies of the Campus Drinking Fountains of North Texas State Teachers College Denton, Texas: "In order to gain an adequate idea of the sanitary condition of the drinking fountains on the North Texas State Teachers College campus, it was found necessary to approach these bacteriological studies from a seasonal point of view."--1.
A Bacteriological Survey of a Freshwater Reservoir: In this study organisms that can be subcultured from lake water, using a prescribed procedure, limit, to an extent, the population, or portions of the population, that can be monitored. In essence, what is taking place is that a set of conditions is set forth and a study is made of the bacteria that will grow under these prescribed conditions.
Bentric Algae of Selected Thermal Springs of Yellowstone National Park: The purpose of this investigation was to characterize the population dynamics of the benthos of selected pristine thermal springs.

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