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  Partner: UNT Libraries
 Degree Discipline: Biology
 Collection: UNT Theses and Dissertations
The Mechanism of Action of Immune Guinea Pig Serum in Staphylococcus Infection in Mice
It is the aim of this work to study the role, if any, of C'4 in the immune response against Staphylococcus aureus infections. digital.library.unt.edu/ark:/67531/metadc108010/
Biological Indices of Stream Pollution
A thorough biological survey and evaluation is a lengthy and expensive project. The number of technically trained persons required prevent its use by most public agencies. Since public health departments are the groups most frequently concerned with measuring the effects of pollution in streams, a need exists for a simplified method of sanitary survey. Recognizing this need, the Texas Heal Department assigned the writer the problem of devising a method of survey which would require a field party of two and could be conducted entirely from a mobile field laboratory. digital.library.unt.edu/ark:/67531/metadc130298/
An Investigation of Methods for the Concentration of Chemical Compounds Produced by Actinomycetes and Their Relation to Tastes and Odors in Municipal Water Supplies
It is the purpose of this investigation to improve upon techniques to enhance the taste of municipal water and attempt to devise one that could desorb the compounds from carbon in an unaltered state. It was decided that the odor character would be the criterion used to decide if the eluted compounds were the same as those in the raw water. digital.library.unt.edu/ark:/67531/metadc107928/
The Incidence of Colon Bacteria on the Hands of North Texas State Teachers College Students
This investigation has for its aim the determination, in a measure, of the degree of personal sanitation existent among students at North Texas State Teachers College, Denton, Texas. digital.library.unt.edu/ark:/67531/metadc53434/
The Relationship of Light Wave Length to Tissue Differentiation in Sunflower Seedlings
The purpose of this study is to determine the relationship of light wave length to tissue differentiation in sunflower seedlings. digital.library.unt.edu/ark:/67531/metadc107922/
Microbial Utilization of a Certain Hydrocarbon Insecticide
This problem includes, first, the isolation and identification of microorganisms which utilized the hydrocarbon insecticide as a sole source of carbon and energy; second, a determination of the effect on plants sprayed with the hydrocarbon medium as compared with the effect on plants sprayed with the hydrocarbon medium containing a good growth of hydrocarbon-utilizers; and third, a determination of the ability of laboratory stock cultures of organisms to utilize or remain alive in the hydrocarbon medium. digital.library.unt.edu/ark:/67531/metadc130266/
Taxonomic Analysis of Marine Actinomycetic Isolates
Though this current study was initiated independently and was not a test laboratory for the taxonomic sub-committee's evaluative program, the problem outlined in this treatise was also designed in an effort to test certain characteristics of the actinomycetes of both a biochemical and morphological nature. This problem employed methods that might absolve or establish certain criteria for taxonomic use in the group of actinomycetes. digital.library.unt.edu/ark:/67531/metadc108052/
Treatment of Akr Mouse Leukemia with Specific Rabbit and Mouse Antiserum
This work is concerned with a study of the role of complement and antibodies in the serum of rabbits and of a non-susceptible strain of mice in the protection of Akr mice injected with active Akr tumor cells. digital.library.unt.edu/ark:/67531/metadc108050/
Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method
The present study may be considered endocrinological and radiobiological in nature. The endocrinology phase was concerned with studying the changes in endocrine function following the application of a stress agent. X-irradiation was chosen as the stressor in order to determine any difference in effect of this stressor from others which have been studied, e. g. heat, cold, metabolic poisons. Liver slices taken from rats at various time intervals following whole body X-irradiation were tested for their ability to metabolize hydrocortisone from a Krebs-Ringer solution. digital.library.unt.edu/ark:/67531/metadc108062/
The Effects of Heparin on the Development of Resistance to Antibiotics by Staphylococcus Aureus
Since heparin combines with some antibiotics to decrease the toxicity of the antibiotic to the patien, the purpose of this investigation is to determine whether it has any effect upon the development of resistance to antibiotics by Staphylococcus aureus. digital.library.unt.edu/ark:/67531/metadc108264/
Studies on the Nutrition of Rhodospirillum Rubrum
The purpose of this study has been to examine the nutritional requirements of Rhodospirillum rubrum and, on the basis of the results, develop a culture medium which could be use to promote more rapid and abundant growth facilitating the laboratory cultivation and observations of the properties so exhibited. digital.library.unt.edu/ark:/67531/metadc107978/
Morphological and Physiological Changes in Micrococcus Pyogenes Var. Aureus during Development of its Resistance to Terramycin
The problem in this investigation consists of, first, the procurement of several strains of Micrococcus pyogenes var. aureus; second, the comparison of the degree and rate of development of resistance of these organisms to terramycin; and, third, to study the morphological and physiological changes which occur during the development of resistance. digital.library.unt.edu/ark:/67531/metadc130350/
The Seasonal Cycle in the Testis of the Large-Mouthed Black Bass, Huro Salmoides, (Lacepede)
Study to determine the seasonal changes in histological and cytological structures in the testis of Huro Salmoides (Lacepede), the large-mouthed black bass. digital.library.unt.edu/ark:/67531/metadc53337/
Chemical and Physiological Properties of an Antibiotic Produced by a Variant of the Penicillium Notatum-Chrysogenum Group
The present study of the production and properties of an antibiotic was undertaken in the hope that some useful information could be contributed to a long-range investigation program being carried on at North Texas State Teachers College. This program is concerned with the production of a useful antibiotic from a variant of the Penicillium notatum-chysogenum group of molds. digital.library.unt.edu/ark:/67531/metadc70386/
The Effect of Repeated Antigen Injections on the C' and C'4 Titers in Guinea Pig Serum
In this study the effects of repeated antigen injections on total complement (C') and C'4 of guinea pig serum were investigated to determine if constant antigenic stimulation would show changes in the C' and C'4 titers. Attempts were also made to correlate any changes with variations in antibody titers during the repeated antigen injections. digital.library.unt.edu/ark:/67531/metadc130458/
The Effect of Avidin Injected Intraperitoneally on the Course of Leukemia in the Mouse
The current work was undertaken to test the ability of avidin to affect the course of leukemia when administered intraperitoneally. digital.library.unt.edu/ark:/67531/metadc108118/
Relation of Dosage of Antiserum to Protection in Mouse Leukemia
It is the purpose of this paper to attempt to confirm the work of Schlagenhauf and Ingebrigsten and to show the effects of repeated injections of guinea pig serum immunized with various strains of mouse leukemia. digital.library.unt.edu/ark:/67531/metadc108124/
Colonial Variations of a Mucoid Strain of Staphylococcus Aureus
It was the purpose of the present investigation to demonstrate the selection of a variant of one strain of Staphylococcus aureus by altering certain environmental factors, and to study that variant as to biochemical activities and capsule formation. digital.library.unt.edu/ark:/67531/metadc108202/
The Effects of Media Constituents Upon the Growth and Pigment Production of Micrococcus Flavus, Micrococcus Roseus, Micrococcus Subcitreus, and Sarcina Citrea
This investigation has for its aim the explanation of growth and pigment production of Micrococcus flavus, Micrococcus roseus, Micrococcus subvitreus, and Sarcina citrea by the addition of various nutrient test materials to a standard culture medium. digital.library.unt.edu/ark:/67531/metadc75621/
The Vegetation of the Austin Chalk Formation of Collin County, Texas
No Description digital.library.unt.edu/ark:/67531/metadc75631/
The Effect of Short Term Immobilization and Drug Induced Muscle Atony on the Blood and Urine Chemistry of Dogs
This thesis investigates immobilization and its complications in dogs. digital.library.unt.edu/ark:/67531/metadc130568/
The Effect of Media Constituents on Growth and Pigment Production of Mycobacterium Phlei, Pseudomonas Fluorescens, Pseudomonas Aeruginosa, and Staphylococcus Citreus
Little is known concerning the production and significance of bacterial pigments. There is seemingly an open field for studying the effects produced by varying the nutritive content of culture media upon which organisms are grown. This has led to an especial interest in, and the purpose of this investigation. digital.library.unt.edu/ark:/67531/metadc75634/
The Effects of Selected Algicides and Some Coordination Complexes upon the Apparent Photosynthesis of Chlorella Pyrenoidosa
Many experiments have been performed with the Warburg apparatus, or variations of this manometric technique, since Warburg's experiments (52, 53) where the effects of cyanides upon dark reactions and of urethanes upon light reactions of photosynthesis were demonstrated. The same basic techniques were utilized in this research in attempting to determine the effects of some coordination complexes upon the apparent photosynthetic rate of Chlorella pyrenoidosa. A second goal of the present paper was to investigate the potential of the Warburg apparatus as a tool for screening algicidal compounds. digital.library.unt.edu/ark:/67531/metadc130600/
Pressure-Flow Relationships in the Isolated Cerebral Circulation of the Dog
The purpose of this investigation is to determine the pressure-flow relationships in the isolated canine cerebral vascular bed. These relationships in vessels which supply an organ surrounded by bone are theoretically influenced by limiting factors which are not present in those vessels surrounded by soft tissue. An evaluation of the extent of these limitations is essential to thorough understanding of the dynamics of the cerebral vascular bed. digital.library.unt.edu/ark:/67531/metadc163848/
Stream water quality corridor assessment and management using spatial analysis techniques: Introduction, evaluation, and implementation of the WQCM model.
The rapid development of once-rural landscapes often produces detrimental effects on surface water quality entering local reservoirs through vulnerable stream channels. This study presents a methodology that incorporates geographic information systems (GIS) and remote sensing techniques for the creation of a stream corridor evaluation mechanism, coined the water quality corridor management (WQCM) model. Specifically, the study focuses on determining the viability of the WQCM model in assessing the stream corridor conditions within a northern Denton County pilot study region. These results will aid in the prediction and evaluation of the quality of stream water entering reservoirs that serve as the primary drinking water source for local municipalities. digital.library.unt.edu/ark:/67531/metadc3976/
Thermal Identification of Clandestine Burials: A Signature Analysis and Image Classification Approach
Clandestine burials, the interred human remains of forensic interest, are generally small features located in isolated environments. Typical ground searches can be both time-consuming and dangerous. Thermal remote sensing has been recognized for some time as a possible search strategy for such burials that are in relatively open areas; however, there is a paucity of published research with respect to this application. This project involved image manipulation, the analyses of signatures for "graves" of various depths when compared to an undisturbed background, and the use of image classification techniques to tease out these features. This research demonstrates a relationship between the depth of burial disturbance and the resultant signature. Further, image classification techniques, especially object-oriented algorithms, can be successfully applied to single band thermal imagery. These findings may ultimately decrease burial search times for law enforcement and increase the likelihood of locating clandestine graves. digital.library.unt.edu/ark:/67531/metadc33201/
Endocannabinoid System in a Planarian Model
In this study, the presence and possible function of endocannabinoid ligands in the planarian is investigated. The endocannabinoids ananadamide (AEA) and 2-arachidonoylglycerol (2-AG) and entourage NAE compounds palmitoylethanolamide (PEA), stearoylethanolamide (SEA) and oleoylethanolamide (OEA) were found in Dugesia dorotocephala. Changes in SEA, PEA, and AEA levels were observed over the initial twelve hours of active regeneration. Exogenously applied AEA, 2-AG and their catabolic inhibition effected biphasic changes in locomotor velocity, analogous to those observed in murines. The genome of a close relative, Schmidtea mediterranea, courtesy of the University of Utah S. med genome database, was explored for cannabinoid receptors, none were found. A putative fatty acid amide hydrolase (FAAH) homolog was found in Schmidtea mediterranea. digital.library.unt.edu/ark:/67531/metadc33188/
Use of Geographic Information System and Remote Sensing Technologies to Describe Mosquito Population Dynamics in the Ray Roberts Greenbelt, Denton County, Texas
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A population survey was conducted from April through September 2002 on mosquito species occurring on the Ray Roberts Greenbelt, a riparian corridor used for public recreation on the Elm Fork of the Trinity River, in Denton County, Texas. ArcGIS software was used to set up a stratified random sampling design based on habitat parameters. Multivariate analyses of sampling data and climatic variables were used to describe spatial and temporal patterns of mosquito species. A total of 33 species were collected during this study belonging to the following genera: Aedes, Anopheles, Coquillettidia, Culex, Mansonia, Ochlerotatus, Orthopodomyia, Psorophora, Toxorhynchites, and Uranotaenia. Seasonal distributions of the dominant species revealed population fluctuations. Aedes vexans was the primary species collected in April and May, occurring in low numbers throughout the rest of the sampling period. Psorophora columbiae reached its highest population density in June, with a smaller peak occurring in late July. Present from May through the end of September, Culex erraticus was the most abundant species collected with major peaks in mid-June and the end of July. Abundance of Culex salinarius followed the same general trend as that for Cx. erraticus, but with smaller numbers. The specimens were tested for a variety of arboviruses by the Texas Department of Health. One pool of Cx. erraticus and Cx. salinarius, collected in August 2002, tested positive for West Nile virus. Variables that were important factors for determining dominant species abundance were temperature, wind speed, rain accumulation occurring one-week and two-weeks prior to sampling, number of day since last rain event, dew point, and average canopy coverage. digital.library.unt.edu/ark:/67531/metadc4160/
The aquatic insect communities of Holbrook Creek and Cochetopa Creek in Colorado.
The first objective for this problem in lieu of thesis project was to gather, identify to the lowest practical taxonomic level and organize all available aquatic insects collected from high altitude Colorado aquatic systems during the summers of 1994, 1996, 1998, and 2002 for the University of North Texas Environmental Science Field Course (BIOL 5650). The curated collection will be housed in the Elm Fork Natural History Museum, located at the University of North Texas. The second objective was to provide a summary and discussion of the occurrence and distribution of the aquatic insects collected from Mt. Blanca in 1994, 1996, and 1998 and to create a taxa list of aquatic insects collected from Cochetopa Creek during the summer of 2002. digital.library.unt.edu/ark:/67531/metadc4381/
Applications of remote sensing and GIS to modeling fire for vegetative restoration in Northern Arizona
An accurate fire model is a useful tool in predicting the behavior of a prescribed fire. Simulation of fire requires an extensive amount of data and can be accomplished best using GIS applications. This paper demonstrates integrative procedures of using of ArcGIS™, ERDAS Imagine™, GPS, and FARSITE© to predict prescribed fire behavior on the Kaibab-Paiute Reservation. ArcGIS was used to create a database incorporating all variables into a common spatial reference system and format for the FARSITE model. ArcGIS Spatial Analyst was then used to select optimal burn sites for simulation. Our predictions will be implemented in future interagency efforts towards vegetative restoration on the reservation. digital.library.unt.edu/ark:/67531/metadc4323/
Determination of Dissociation Constants for GABAA Receptor Antagonists using Spontaneously Active Neuronal Networks in vitro
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Changes in spontaneous spike activities recorded from murine frontal cortex networks grown on substrate-integrated microelectrodes were used to determine the dissociation constant (KB) of three GABAA antagonists. Neuronal networks were treated with fixed concentrations of GABAA antagonists and titrated with muscimol, a GABAA receptor agonist. Muscimol decreased spike activity in a concentration dependent manner with full efficacy (100% spike inhibition) and a 50% inhibitory concentration (IC50) of 0.14 ± 0.05 µM (mean ± SD, n=6). At 10, 20, 40 and 80 µM bicuculline, the muscimol IC50 values were shifted to 4.3 ± 1.8 µM (n=6), 6.8 ± 1.7 µM (n=6), 19.3 ± 3.54 µM (n=10) and 43.5 µM (n=2), respectively (mean ± SD). Muscimol titration in the presence of 10, 20, 40 µM of gabazine resulted in IC50s values of 20.1 (n=2), 37.17 (n=4), and 120.45 (n=2), respectively. In the presence of 20, 80, and 160 µM of TMPP (trimethylolpropane phosphate) the IC50s were 0.86 (n=2), 3.07 (n=3), 6.67 (n=2) µM, respectively. Increasing concentrations of GABAA antagonists shifted agonist log concentration-response curves to the right with identical efficacies, indicating direct competition for the GABAA receptor. A Schild plot analysis with linear regression resulted in slopes of 1.18 ± 0.18, 1.29 ± 0.23 and 1.05 ± 0.03 for bicuculline, gabazine and TMPP, respectively. The potency of antagonists was determined in terms of pA2 values. The pA2 values were 6.63 (gabazine), 6.21 (bicuculline), and 5.4 (TMPP). This suggests that gabazine has a higher binding affinity to the GABAA receptor than bicuculline and TMPP. Hence, using spike rate data obtained from population responses of spontaneously active neuronal networks, it is possible to determine key pharmacological properties of drug-receptor interactions. digital.library.unt.edu/ark:/67531/metadc4911/
The Life History and Contributions to the Ecology of Camelobaetidius variabilis Wiersema 1998 (Ephemeroptera: Baetidae) in Honey Creek, Oklahoma
A study of the life history and ecology of Camelobaetidius variabilis was conducted in Honey Creek, OK from February 2003-April 2004. Nymph development was assessed using changes in external morphology. Laboratory reared nymphs were used to calculate number of degree days to complete development (772 degree days at 20.8° C ±.38° C), which was used to determine voltinism. Field collected nymph microhabitat distribution was used in assessing microhabitat distribution. Nymphal thermoregulation was assessed during the winter and spring by comparing nymphal numbers present in shaded and un-shaded habitats. Camelobaetidius variabilis nymphs showed preference for algal microhabitats during the spring and leaf packs in the winter. Nymphs inhabited leaf packs to increase metabolic rate during the winter. Increased temperatures aid in development of nymphs. Camelobaetidius variabilis exhibited a multivoltine life cycle with six overlapping generations. digital.library.unt.edu/ark:/67531/metadc4944/
Pyrimidine Enzyme Specific Activity at Four Different Phases of Growth in Minimal and Rich Media, and Concomitant Virulence Factors Evaluation in Pseudomonas aeruginosa
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Pseudomonas aeruginosa is a Gram-negative rod, aerobic, non-fermenting, oxidase positive, pigment producing, and nutritionally versatile bacterium. Infections by P. aeruginosa are the most important cause of morbidity and mortality in immunocompromised patients, given virulence factor production that suppresses antibiotic therapy and promotes persistent infection. This research is the first comprehensive report of the pyrimidine biosynthetic pathway for all phases of growth in minimal and rich media coupled with the evaluation of virulence factor production of P. aeruginosa in comparison to four other bacterial species (Pseudomonas putida, Pseudomonas fluorescens, Burkholderia cepacia, and Escherichia coli wild-type strains). Cellular growth and passing genetic information to the next generation depend on the synthesis of purines and pyrimidines, the precursors of DNA and RNA. The pyrimidine biosynthetic pathway is essential and found in most organisms, with the exception of a few parasites that depend upon the pyrimidine salvage pathway for growth. Both the pyrimidine biosynthetic and salvage enzymes are targets for chemotherapeutic agents. In our laboratory, research on pyrimidine auxotrophic mutants showed the role of the pyrimidine biosynthetic pathway and its intermediates on P. aeruginosa metabolism and impaired virulence factors production. The present research shows that pyrimidine enzymes are active in all phases of growth, including the production of two forms of ATCase in the late log phase in P. aeruginosa. This finding may be explained by the displacement of the inactive PyrC' by the active PyrC or PyrC2 to form a new and larger pyrBC encoded ATCase. Pseudomonas aeruginosa wild-type appears to produce by far the most virulence factors, haemolysin, iron chelation, rhamnolipid, adherence, and three types of motility (swimming, swarming, and twitching) investigated in this study, when compared to the other four wild-type strains. Growth analysis was carried out as typically done in minimal medium but also in rich medium to simulate conditions in the blood and lung tissues of humans as P. aeruginosa infections develop. digital.library.unt.edu/ark:/67531/metadc4918/
A Physiological Age-Grading System for Female Hydrellia pakistanae Deonier (Diptera: Ephydridae)
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Conflicting opinions about the effectiveness of H. pakistanae as a biological control agent for hydrilla prompt researchers to find a method for assessing the fly's success. Developing a physiological age-grading system for the fly using ovarian morphology to detect changes in reproductive activity is useful for evaluating reproductive status of the fly in field populations. Changes in the appearance of follicular relics in ovaries with oviposition provide a reliable method to estimate fecundity. Characteristics of follicular relics were used to develop a system with eight physiological age classes, three nulliparous and five parous. Changes that occur in the fat body were used to assist in classification of nulliparous females or those with low egg counts. digital.library.unt.edu/ark:/67531/metadc3280/
Enallagma civile (Odonata: Coengrionidae) life history and production in a west Texas playa
A life history and productivity study of Enallagma civile was conducted in a playa that was located in the southern High Plains of Texas. Other odonates were also studies to identify their contributions to the habitat. digital.library.unt.edu/ark:/67531/metadc3157/
Autoradiographic localization of carbachol-induced second messenger response in the rat spinal cord following inflammation.
This study examined central mechanisms of persistent pain using an autoradiographic technique to localize phosphoinositide hydrolysis (PI) in the rat spinal cord dorsal horn. The lateral half of laminae I-II showed the highest levels of baseline PI turnover and carbachol-stimulated PI turnover in normal animals as well as after inflammation. Inflammation resulted in increased baseline PI turnover in this region of the ipsilateral (76%) and contralateral (65%) dorsal horns. Carbachol increased PI turnover in this region in normal rats (55%) and following inflammation (ipsilateral: 46%, contralateral: 45%). The absolute magnitudes of these increases were 1.85, 2.71, and 2.51 nCi/mg, respectively. The results of this study demonstrate the involvement of PI turnover in neural mechanisms of persistent pain, and provide evidence for the involvement of cholinergic systems in this process. Because spinal cholinergic systems have been reported to be anti-nociceptive, the present results appear to reflect an upregulation of anti-nociceptive activity in response to inflammation. Thus, the spinal cholinergic system may be a regulatory site within the anti-nociceptive pathway, and may provide an attractive target for the development of new therapeutic agents. digital.library.unt.edu/ark:/67531/metadc3125/
College Freshman Biology Two Semester Course: Integrating Deep Processing Teaching Techniques
Development of a college level freshman biology course was undertaken in response to government reports that American students have fallen behind students of other countries in the area of the sciences. Teaching strategies were investigated to accomplish two objectives, to define essential academic material to include in the course and to investigate teaching techniques that would increase deep processing of the information. An active process that consisted of applying the cognitive information to solving problems or developing answers to questions was defined as critical thinking. Critical thinking was incorporated into the course by the use of case studies. digital.library.unt.edu/ark:/67531/metadc3158/
Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio).
Superior swimming stamina in adult fish is presumably passed on to their offspring, but the ontogeny of the appearance of superior stamina and the requisite enhanced cardio-respiratory support for locomotion in larval fishes has not been determined. Is the expression of the suite of parental traits enabling superior swimming stamina in their offspring dependent upon their achieving juvenile/adult morphology, or does it appear earlier in their larvae? To answer this, adults were classified into three groups based on swimming stamina, followed by measurement of length, mass, and width. Larval offspring from the two parental groups -high stamina larvae (HSL) and low stamina larvae (LSL)- were reared at 27°C in aerated water (21% O2). Routine and active heart rate, routine and active mass specific oxygen consumption were recorded through 21dpf, and cost of transport (COT) and factorial aerobic scope were derived from oxygen consumption measurements. Routine heart rate at 2dpf of LSL was 164 ± 1 b·min-1, compared to only 125 ± 2 b·min-1 for HSL. Routine heart rate subsequently peaked at 203 ± 1 b·min-1 at 5dpf in the HSL group, compared to 207 ± 1 b·min-1, at 4dpf in the LSP larvae. Active heart rate at 5 dpf of LSL was 218 ± 2 b·min-1 compared to 216 ± 2 b·min-1 for HSL. Active heart rate increased slightly to 227 ± 2 b·min-1 for LSL before decreasing again, while active heart rate remained relatively constant for HSL. Routine O2 consumption at 2dpf of HSL was 0.09 μmol·mg-1·hr-1, compared to 0.03 μmol·mg-1·hr-1 in LSL. Routine O2 consumption subsequently peaked at 0.70 μmol·mg-1·hr-1 at 9dpf in the HSL, compared to 0.71 μmol·mg-1·hr-1, at 9dpf in the LSL. These values dramatically decreased before leveling off at around 0.20 μmol·mg-1·hr-1 and 0.15 μmol·mg-1·h-1, respectively. Active O2 consumption at 5dpf for HSL was 0.38 μmol·mg-1·hr-1, compared to 0.57 μmol·mg-1·hr-1 for LSL. Active O2 consumption subsequently peaked at 0.97 μmol·mg-1·hr-1 at 10dpf in HSL, compared to 1.19 μmol·mg-1·hr-1 at 7dpf in LSL. These values also dramatically decreased and leveled off. Significant differences (p < 0.05) in heart rate and oxygen consumption persisted through 21dpf. The onset of differences observed in routine and active heart rate in early larvae, correlated with parent stamina, show that juvenile or adult features are not required as a precondition for the emergence of phenotypic physiological differences. digital.library.unt.edu/ark:/67531/metadc3669/
Bacterial challenge in Lumbricus terrestris: A terrestrial invertebrate immunotoxicity model.
A bacterial challenge assay was developed utilizing the earthworm, Lumbricus terrestris, in order to assess potential immunotoxic effects from exposure to specific polychlorinated biphenyl congeners. Earthworms were inoculated with Aeromonous hydrophila, establishing a 10-day LD50. In vitro assays for effects of PCBs on phagocytosis agreed with mammalian studies, demonstrating potent suppression of phagocytosis by the non-coplanar PCB congener 138 and no suppression by the coplanar congener 126. However, when the effects of the two PCB congeners were evaluated for suppression of resistance to a whole animal infection challenge assay, coplanar PCB 126 decreased the ability of L. terrestris to withstand infection while non-coplanar PCB 138 did not. digital.library.unt.edu/ark:/67531/metadc3640/
Classification of toolmark surfaces on zipper teeth
This study proposes the classification of the toolmark under the heads of zipper teeth as a subclass characteristic as outlined by the Association of Firearm and Toolmark Examiners (AFTE). Two separate cases in which zipper teeth were found at crime scenes prompted this study. Brass zipper teeth manufactured by YKK were taken from 20 pairs of jeans and studied using a Reichert comparison microscope at 4X power. Photographs were taken and over 750 comparisons made. It was found that the toolmarks on each side on the 20 zippers were unique and independent of all other sides. The observations made in this study indicate that classifying zipper teeth toolmarks as a subclass characteristic is valid. digital.library.unt.edu/ark:/67531/metadc2248/
Syllabus Outline for Genetics Lecture and Laboratory
This work is intended to be used as a teaching tool in conjunction with the text cited. It is written in outline format, highlighting the major concepts of each pertinent chapter. In this format, the concepts can be expanded upon at the discretion of the instructor. This work is to be used as a guide for lecture. The basic concepts contained in the outline are in such a format as to be able to work in more information regarding the subject matter if needed. The instructor can work from this outline as a starting point. Major topics in the chapters are highlighted, making lecture notes for the instructor easier to do. digital.library.unt.edu/ark:/67531/metadc2241/
Syllabus for Advanced Placement Biology
The purpose of this syllabus is to provide a working copy to those teachers of the advanced placement biology course taught at the high school level. Reference materials used were the Texas Education Agency ( TEA ) approved Campbell text Biology and the College Board's, Advanced Placement Biology Laboratory Manual. The syllabus is divided into major topics with outlined notes and includes laboratory exercises as recommended by the College Board. The AP biology course is intended to be equivalent to college biology. College freshman biology courses can differ among colleges and among teachers within the same college. This syllabus is intended to serve as an aid to AP teachers, to cover the topics and experiments as set out by the College Board, and to the high school student, the necessary material to successfully complete the AP examination while providing freshman biology equivalence. digital.library.unt.edu/ark:/67531/metadc2203/
Assimilation of Inorganic Nitrogen by Aquatic Actinomycetes
It was the purpose of this investigation to present laboratory data concerning the assimilation of inorganic nitrogen by the aquatic actinomycetes. The strains of aquatic actinomycetes under consideration represented a cross section of those currently under culture at North Texas State University. digital.library.unt.edu/ark:/67531/metadc108150/
Some Relationships between Certain Aquatic Actinomycetes and Bacillus Cereus
The purpose of this investigation is to determine if there was a metabolic relationship between the actinomycetes and the gram positive, spore-forming becilli in surface waters, and, if such a relationship was evident, to relate the association to the disappearances of typical actinomycete tastes and odors from waters. digital.library.unt.edu/ark:/67531/metadc108230/
Autoimmunity of Periodontitis
The purpose of this investigation is to determine if auto-antibodies are demonstrable in inflammatory periodontal disease using methods other than those of Novotny. digital.library.unt.edu/ark:/67531/metadc108240/
Effects of X-Irradiation on Water and Ion Flux in Isolated Roots
Due to the relatively small amount of work concerning radiation, it was thought feasible to use ionizing radiation as a tool to study the relationship between water and salt flux in isolated root systems. Moreover, the more quantitative potometric method lends itself well to such a study, since the two processes can be followed simultaneously. the aims of this study, therefore, were 1) to determine effects of X-irradiation on water, calcium and potassium flux in excised onion roots; 2) to determine the dose levels required for producing permeability changes; 3) to investigate the nature of radiation damage to root systems; and 4) to attempt to shed light on the relation between water transport and ion movement in root systems. digital.library.unt.edu/ark:/67531/metadc163902/
Studies of Fungal Antagonism in a Norfolk Fine Sand, Denton County, Texas
This investigation has had for its primary purpose the noting of the incidence of antagonists as occurring in a Denton County, Texas, Norfolk Fine Sand. A further interest has been the determination of the effect of these antagonists upon known beneficial soil organisms. digital.library.unt.edu/ark:/67531/metadc83403/
Microflora of Frozen Fruits and Vegetables Dispensed from Grocery Stores in Denton, Texas
This investigation was carried out in order to determine whether or not blanching and freezing tends to reduce the incidence of bacteria, particularly those of the coliform group, and also the yeasts and molds. digital.library.unt.edu/ark:/67531/metadc83405/
Identification of Streptomyces Species Using Fluorescent Antibody-Membrane Filter Techniques
It is the purpose of this investigation to modify existing methods in an attempt to provide a rapid identification of members of the genus Streptomyces. digital.library.unt.edu/ark:/67531/metadc130876/
Effects of pH and Substrate on Growth of Escherichia Coli and Pseudomonas Aeruginosa in Mixed Continuous Culture
The express purpose for doing this project was to develop methods for the continuous culture of E. coli and P. aeruginosa as a mixed population, and to apply these methods in studying the effects of pH and substrate upon the growth of these two organisms in mixed continuous culture. digital.library.unt.edu/ark:/67531/metadc108256/
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