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Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764

Description: The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which the substrate is initially detoxified by complexation to an appropriate lig… more
Date: August 2000
Creator: Parab, Preeti
open access

Scientific Considerations of Olestra as a Fat Substitute

Description: Olestra is, a sucrose polyester, a noncaloric fat substitute, made from sucrose and several fatty acid esters. It has been approved by the FDA as a food additive used in preparing low-fat deep-frying foods such as savory snacks. Available literature on olestra was evaluated that had both positive and negative connotations. Clinical trials in numerous species of animals including humans were conducted to determine if olestra would affect the utilization and absorption of macro- and micronutrient… more
Date: December 1999
Creator: Rattagool, Kullakan
open access

Aquatic Heterotrophic Bacteria Active in the Biotransformation of Anthracene and Pentachlorophenol

Description: Dominant genera of bacteria were isolated from three river waters during anthracene and pentachlorophenol biotransformation studies. The genera Pseudomonas, Acinetobacter, Micrococcus, Chromobacterium, Alcaligenes, Azomonos, Bacillus, and Flavobacterium were capable of biotransforming one or both of these compounds. These isolates were subjected to further biotransformation tests, including river water and a basal salt medium with and without additional glucose. The results of these experiments… more
Date: August 1985
Creator: Entezami, Azam A. (Azam Alsadat)
open access

A Study of the Water-Soluble Antigens from Virulent and Attenuated Biotypes of Brucella abortus

Description: Through chemical analysis and ion exchange chromatography of watersoluble antigens, this investigation supports the view that the majority of differences between the biotypes are quantitative. It was also found that strains demonstrate distinct, qualitative differences when compared to the attenuated strain 19 by immunodiffusion and thin-layer polyacrylamide gel, isoelectric focusing. These differences include the presence of antigens on virulent strains that are absent on strain 19. In additio… more
Date: May 1977
Creator: Brodeur, Richard D.
open access

The Detection of Poliovirus in Denton Sewage by Immunofluorescence and Immunodiffusion Techniques

Description: Several final sewage effluents from the Denton Disposal Plant were demonstrated to contain Poliovirus types II and III. Pleated encapsulated filters at pH3.5 enhanced the recovery of the Poliovirus at a higher tier in comparison with nitrocellulose filter (Millipore) and glass fiber filter of pore size 0.45u. This thesis explores problems that face us today in our quest to eliminate viral pathogens from the natural and waste water needed for human, domestic, and industrial consumption. Prelimin… more
Date: August 1975
Creator: Olaiya, Felix Ayodele
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