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open access

Identification of Glycine as the Factor in Peptone Which Induces Pleomorphism in Azotobacter Vinelandii

Description: The rigid peptidoglycan layer of the cell wall is responsible for maintaining the structural integrity of bacteria. Antibiotics such as penicillin exert their anti-bacterial effect by inhibiting synthesis of peptodoglycan, and enzymes such as lysozyme destroy cell integrity by hydrolyzing specific bonds in the interior of this macromolecule. Defective cells can no longer withstand the high turgor pressure within the cell because they are no longer protected by a rigid wall and tend to become fr… more
Date: December 1972
Creator: Rosenthal, Raoul Simon
open access

Isolation and Characterization of Malic Enzyme from Ascaris suum

Description: A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. The fractionation method yields relatively large quantities of the enzyme,with a specific activity of fifteen moles of malate converted to pyruvate and carbon dioxide per min per mg at 25º. Homogeneity was established with analytical ultracentrifugation, zone electrophoresis, isoelectric focusing, and rechromatography. The molecular weight of the enzyme was 250,000, and it is dissociated… more
Date: December 1972
Creator: Fodge, Douglas W.
open access

Linkage Relationships of Greenbug Resistance in Barley, Hordeum Vulgare L.

Description: The linkage relationship and arm location of the gene for greenbug resistance in the variety Will was determined by using primary trisonomics and tertiary trisomic homozygous translocations. The gene for greenbug resistance was found to be on linkage group 1 by using primary trisonomics. The gene was located on the cetromere segment of the Tl-6a translocation by using a tertiary trismoic homozygous for greenbug resistance. The data further substantiates the feasibility of using trisomics in pla… more
Date: December 1972
Creator: Gardenhire, James H.
open access

Carbon Flux in Reservoir Sediments

Description: The central objective of the study was to fractionate sedimenting organic materials according to their source (allochthonous or autochthonous) and ultimately to determine the degree of biodegradability of contributions from either source with particular reference to activities at the mud-water interface.
Date: May 1973
Creator: Newton, Charles Eugene
open access

Effects of a Methylcholanthrene-Induced Lymphosarcoma on Various Tissues of DBA/1J and Swiss White Mice

Description: This investigation was concerned with characterizing effects of this tumor line on lipid metabolism in DBA/lJ mice and serum protein levels and cellular changes in DBA/lJ and Swiss white mice. Total lipids, lipid phosphorus, neutral lipids, and changes in fatty acids were determined in liver, spleen, skin, and tumor of DBA/lJ mice bearing the lymphosarcoma at various days after injection of tumor cells.
Date: May 1973
Creator: Lindsey, Terri Jay
open access

Trophogenic Ecology of Selected Southwestern Reservoirs

Description: Three north central Texas reservoirs (Grapevine Reservoir, Lake Ray Hubbard, Lewisville Reservoir) were investigated in order to characterize the nutritional ecology, phytoplankton community structure, and primary productivity within the trophogenic zones of each. Emphasis was placed on elucidating the relative influences of the major nutrients (C, N, P, Fe, Si) and various other physico-chemical parameters on phytoplankton community biomass, structure, and productivity. Extensive physical, che… more
Date: May 1973
Creator: McDaniel, Michael David
open access

Biological Nitrogen Fixation in Two Southwestern Reservoirs

Description: This investigation has determined the presence of biological nitrogen fixation in two reservoirs in the southwestern United States: Lake Arlington and Lake Ray Hubbard. Subsequent tests have gathered baseline data on the effects of various biological, chemical, and physical parameters on in situ nitrogen fixation in these reservoirs. Of specific importance is the relationship between nitrogen fixation arid occasional blooms of blue-green algae which produce such problems as testes and odors in … more
Date: August 1973
Creator: Lawley, Gary G.
open access

Biochemical Systematics of the Genus Sophora

Description: Three unusual amino acids, y-amino-n-butyric acid, pipecolic acid, and 4-hydroxypipecolic acid, and an uncommon dipeptide, y-glutamyltyrosine, have been isolated and characterized from the seeds of members of the genus Sophora. Structural proof of these compounds was carried out by paper chromatography, thin-layer chromatography, column chromatography on amino acid analyzer, infrared, nuclear magnetic resonance, mass spectrometry, and C, H, N analysis. The presence and absence of these compound… more
Date: December 1973
Creator: Izaddoost, Mohamed
open access

Evolutionary Genetics of Certain Mice of the Peromyscus boylii Species Group

Description: The genetic structure of 49 natural populations of four species (P. attwateri, P. boylii, P. pectoralis, and P. polius) of the Peromyscus boylii species group was analyzed through application of chromosomal and electrophoretic techniques. Chromosomal variation within and among populations of the boylii species group was analyzed from 178 specimens. Electrophoretic techniques were utilized for the demonstration of variation in enzymes and other proteins encoded by structural loci and applied to … more
Date: December 1973
Creator: Kilpatrick, Charles William
open access

Microbiological Treatment of Wastewater from a Wood-Preserving Plant

Description: This research investigates interacting biological, chemical, and physical factors affecting the efficiency of microbiological wastewater treatment at the W. J. Smith Wood- Preserving Company in Denison, Texas. The treatment process consisted of collecting exhaust boiler water containing unidentified boiler treatment compounds, steam condensate contaminated with preservatives and wood extracts, plant process waters, and rainfall runoff from plant grounds. With a 5-minute residence time, wastewat… more
Date: August 1974
Creator: Ralston, James R.
open access

Biochemical Genetics of the Pocket Gopher Genus Geomys, and its Phylogenetic Implications

Description: Electrophoretic techniques were utilized for the demonstration of variation in 22 proteins from 24 natural populations of four species ( G. bursarius, G. pinetis, G. arenarius and G. personatus ) of the Geomys complex of pocket gophers. Of the 24 structural loci , 19 were considered to be polymorphic. Five of the six esterases contributed greatested to the polymorphism while non-esterase proteins generally showed low values. In the GeoMys complex of pocket gophers in this study,selection appear… more
Date: December 1974
Creator: Penney, Dan F.
open access

Ecological Energetics of the Dobson Fly, Corydalus Cornutus

Description: Rates and energies of consumption (C), egestion (F), assimilation (A), respiration (R), growth (Pg), production of exuviae (Pev), and production of egg masses (Pr) and associated efficiencies, and the effects of seasonal temperature, weight and metamorphic stage upon these factors were examined for a typical individual and cohort of Corydalus cornutus (L.) from a stream in North-Central Texas (330 23'N, 97*5'W). Dobson flies are apparently univoltine in the study area, with 11 larval instars. E… more
Date: December 1974
Creator: Brown, Arthur V.
open access

A Study of the Interaction of Co-Insult Treatments with Methylmercuric Chloride and X-Irradiation and Demonstration of a Peroxide Induced Protective Mechanism

Description: The initial purpose of this work was to investigate the interaction of methylmercuric chloride (MMC) and X-irradiation given as a co-insult upon the rat blood-brain barrier (BBB). The indicators used to determine BBB alterations were mortality and the in vivo tissue uptake of radioactive sulfate administered as 3 5S-sodium sulfate. The results of the interaction studies indicated a neutralization of effects when MMC and X-irradiation were given together. X-irradiation as a single insult general… more
Date: August 1975
Creator: Earhart, James M.
open access

Population Genetics, Karyology, and Morphology of Certain Species of the Peromyscus Truei Group

Description: The systematic relationship of two species of the Peromyscus truei group (P. truei and P. difficilis) was analysed through the application of starch gel electrophoresis, numerical taxonomy, and chromosomal techiques. Of 20 loci examined, 11 were monomorphic in all populations, two exhibited variation in only two populations, and seven loci were polymorphic in two or more populations. The mean number of polymorphic loci per population was 0.186, the mean number of polymorphic loci per individual… more
Date: May 1976
Creator: Hart, Billy Joe
open access

Aspects of the Thermal Ecology of Largemouth Bass (Micropterus salmoides) in North Central Texas

Description: The coefficient of body temperature change (K) ranged from -0.53 to -0.072 for bass weighing 73-1440 g. The double log regression of K on weight was similar to that reported for other poikilotherms (slope = -0.57; R = 0.93). Fingerling bass were eurythermal, being capable of surviving instantaneous temperature changes over a 20 C range at acclimation temperatures of 15, 25 and 30 C and over a 15 C range at acclimation temperatures of 20 and 35 C. Preferred temperatures for adult bass measured i… more
Date: December 1976
Creator: Venables, Barney J.
open access

Effect of 2,450 MHz Microwave Radiation on Microorganisms

Description: The effect of microwave radiation on soil bacteria in situ has been studied in both lab and field conditions. Radiation and thermal profiles show that heterotrophic bacteria, spores, fungi, and actinomycetes were not affected by total microwave radiations over the range 0 to 80 seconds of exposure at a net input of 1 KW of intensity. Nitrogen-fixing bacteria and nitrifying bacteria were also resistant to these doses. The soil microorganisms were inactivated as a function of microwave radiation … more
Date: May 1977
Creator: Wu, Jung Fu
open access

Effects of Phytohormones on Scenedesmus quadricauda

Description: The literature on the effects of phytohormone on algae is clouded with contradictory reports. Reports have been published which substantiate and deny the effects of phytohormones in enhancing the growth and developmental processes in algae. The overall aim of this study was to investigate the response, if any, of the phytohormones indole-3-acetic acid (IAA), gibberellic acid A3 (GA) and kinetin on the physiology of the green alga, Scenedesmus quadricauda. Results obtained for the uptake of 14^C… more
Date: August 1977
Creator: Alam, Mohammad Ihtisham
open access

The Physiology of Azotobacter Vinelandii Cysts

Description: The value of the adenylate energy charge [(ATP)+1/2(ADP)/(ATP)+(ADP)+(AMP)] in Azotobacter vinelandii cells was monitored during growth and germination in flask cultures. The miximal value of 0.88 was attained during mid-log phase; this declined gradually to 0.50 by late stationary phase. When these cultures were transferred to encystment media, the adenylate energy charge decreased to an average value of 0.40 as the vegetative cells encysted and remained unchanged during the next 20 days. Ency… more
Date: December 1980
Creator: Aladegbami, Solomon L.
open access

Taxonomy of the Azotobacteraceae

Description: The classification of the Azotobacteraceae to the level of genus and species has been uncertain since the studies of Beijerinck in 1901. This dissertation represents an effort to establish a system of classification more complete than the one now in use. In this study, both qualitative and quantitative taxonomy were used in order to establish a well founded classification scheme. Qualitative methods included certain important morphological and physiological characteristics, isoenzyme patterns, … more
Date: December 1980
Creator: Chang, Charles Shing
open access

Lizard Tail Autotomy: Some Aspects of its Ecology and Energetics

Description: Autotomy (self-induced loss) of body parts has evolved independently as a predator defense mechanism in several major animal phyla. The mechanism among vertebrates, including 13 of the 20 recognized lizard families, is tail autotomy. Its occurrence among the majority of lizard families offers an excellent opportunity to examine the effects of a major selective force on the ecology and evolution of a group of organisms. The research of this study was designed to examine experimentally (i) the fu… more
Date: December 1981
Creator: Dial, Benjamin Edward
open access

Chemically Active Odorants as Olfactory Probes

Description: The initial step in odor recognition by the nose is the binding of odorant molecules to receptor sites embedded in the dendritic membranes of olfactory receptor cells. Despite considerable interest and experimentation into the nature of these receptor sites, little is known of their specificity to different types of odorant molecules. This lack of knowledge partially stems from the fact that the nature of receptor proteins is most effectively studied when specific and irreversible inhibitors ar… more
Date: May 1982
Creator: Criswell, Darrell W. (Darrell Wayne)
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Lethal and Sublethal Effects of Hemoxidants, Particularly Nitrite, on Selected Aquatic Animals

Description: A research program was developed to investigate basic and applied aspects of toxicity, both lethal and sublethal, of hemoxidants, particularly nitrite, on fish, non-fish aquatic vertebrates, and crayfish. The major objectives of this research were to determine A) acute and sublethal toxicity of nitrite to selected aquatic organisms: 1. aquatic salamander larvae, Ambystoma texanum, 2. swamp crayfish, Procambarus simulans, 3. bluegill, Lepomis macrochirus, 4. bullfrog, tadpoles, Rana catesbiana, … more
Date: May 1982
Creator: Huey, David W. (David Worley)
open access

Postsynthetic Modifications of Glycolytic Enzymes of the Geriatric Immune System and in Fibroblasts from Premature Aging Diseases

Description: During mitogen-induced transformation of human lymphocytes, phosphoglycerate kinase (PGK) exhibits new electrophoretic forms (pl=8.5-8.9). Electrophoresis and electrofocusing showed that the new forms are not due to expression of the autosomally linked isozyme found in semen (PGK-B; pl=9.7). The multiple electrophoretic forms are the result of protease modification of sex-linked PGK-A isozyme.When peripheral lymphocytes from young persons are stimulated in vitro with phytohemagglutinin, a selec… more
Date: August 1982
Creator: Tollefsbol, Trygve O.
open access

The Effects of Freshet Turbidity on Selected Aspects of the Biogeochemistry and the Trophic Status of Flathead Lake, Montana U.S.A.

Description: The present study sought to falsify three distinct hypotheses about how the interactions of freshet clay turbidity with Flathead Lake may be affecting its trophic state: 1) that freshet-derived turbidity causes precipitation of organic detritus from the water column by flocculation and/or coagulation of dissolved and colloidal organic carbon and seston; 2) that flocculated clay-organic detritus complexes become increasingly infested with microbial biomass as they sink through the water column; … more
Date: August 1983
Creator: Stuart, Tom J.
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