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Isolation and Bioinformatic Characterization of Four Novel Bacteriophages from Streptomyces toxytricini

Description: Six initial phage isolates with high titer lysates were obtained using Streptomyces toxytricini B-5426 as the host bacterium. These isolates were named Goby, Toma, Yosif, Yara, Deema, and Hsoos. However, upon completion of the sequencing, it was found that the Yara and Hsoos isolates were identical, as were Goby and Deema. As a result, final analysis was completed on only the four unique isolates. All of the phages mentioned above were isolated from soil samples from different locations. Al… more
Date: May 2019
Creator: Alzaid, Hessah
Item Type: Refine your search to only Thesis or Dissertation
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Autonomic Nerve Activity and Cardiovascular Function in the Chicken Embryo (Gallus gallus)

Description: The goal of this study was to build on the historic use of the avian model of development and also to further the knowledge of autonomic nervous system (ANS) regulation of cardiovascular function in vertebrates. Vasoactive drugs sodium nitroprusside, a vasodilator and phenylephrine, a vasoconstrictor were used to study the correlation of cardiovascular function relationship with nerve activity, both sympathetic and parasympathetic (vagal). Additionally, ANG II was used to assess its effects on … more
Date: December 2018
Creator: Onyemaechi, Clinton
Item Type: Refine your search to only Thesis or Dissertation
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The Role of Thyroid Hormone on the Development of Endothermy in White Leghorn Chickens (Gallus gallus)

Description: As chickens hatch, there is a rapid change in their physiology and metabolism associated with attaining endothermy. It is thought that thyroid hormones (TH) play a major role in regulating developmental changes at hatching. In birds, TH regulates skeletal muscle growth, which has a direct impact on the chick's ability to thermoregulate via shivering thermogenesis. To better understand the role of TH in the timing of hatching, development of thermogenic capacity, and metabolic rate, we manipu… more
Date: August 2018
Creator: Rippamonti, Jessica D.
Item Type: Refine your search to only Thesis or Dissertation
open access

The Role of Transmembrane Protein 59 in Thrombocyte Function and the Effect of MS-222 on Hemostasis in Zebrafish

Description: Transmembrane protein 59 (tmem59) is a gene that encodes a protein involved in autophagy and apoptosis in human. A previous study in zebrafish showed that tmem59 mRNA was several folds higher in thrombocytes than those found in red blood cells (RBCs). Therefore, we hypothesized that tmem59 has a role in thrombocytes function. We injected a hybrid of control vivo-morpholino (cVMO) and tmem59 specific antisense standard oligonucleotide (tmem59SO) into adult zebrafish to knockdown tmem59.This pigg… more
Date: August 2018
Creator: Deebani, Afnan Omar M.
Item Type: Refine your search to only Thesis or Dissertation
open access

Superbursts: Investigation of Abnormal Paroxysmal Bursting Activity in Nerve Cell Networks In Vitro

Description: Superbursts (SBs) are large, seemingly spontaneous activity fluctuations often encountered in high density neural networks in vitro. Little effort has been put forth to define and analyze SBs which are paroxysmal bursting discharges. Through qualitative and quantitative means, I have described specific occurrences of superbursting activity. A complex of paroxysmal bursting has been termed a "superburst episode," and each individual SB is a "superburst event" which is comprises a fine burst stru… more
Date: May 2018
Creator: Suri, Nikita
Item Type: Refine your search to only Thesis or Dissertation
open access

In Vitro Exploration of Functional Acrolein Toxicity with Cortical Neuronal Networks

Description: Acrolein is produced endogenously after traumatic brain injury (TBI) and is considered a primary mechanism for secondary damage occurring after TBI. We are using frontal cortex networks derived from mouse embryos and grown on microelectrode arrays in vitro to monitor the spontaneous activity of networks and the changes that occur after acrolein application. Networks exposed to acrolein exhibit a biphasic response profile. An initial increase in network activity, followed by a decrease to 100% a… more
Date: May 2018
Creator: Durant, Stormy R.
Item Type: Refine your search to only Thesis or Dissertation
open access

Population Dynamics and Community Structure of Mosquitoes (Diptera: Culicidae) Recorded in Denton, Texas from 2005 to 2015

Description: A population survey was conducted on the mosquito species recorded in Denton, Texas for the years of 2005 to 2015. Data used in this project were obtained from an ongoing, long-term surveillance program led by the City of Denton and conducted through the University of North Texas. Research focused on the population dynamics and community structure of mosquitoes collected within urban areas of Denton, Texas in relation to certain environmental variables. A total of 80,837 female mosquitoes were … more
Date: May 2018
Creator: Hambrick, Bethany Lynn
Item Type: Refine your search to only Thesis or Dissertation
open access

Simultaneous Electrophysiological and Morphological Assessment of Impact Damage to Nerve Cell Networks

Description: A ballistic pendulum impulse generator was used to impact networks in primary culture growing on microelectrode arrays. This approach has the advantage of imparting pure tangential acceleration insults (50 to 300 g) with simultaneous morphological and electrophysiological multichannel monitoring for days before and after the impact. Action potential (AP) production, network activity patterns, and cell electrode coupling of individual units using AP waveshape templates were quantified. Network … more
Date: May 2018
Creator: Rogers, Edmond A.
Item Type: Refine your search to only Thesis or Dissertation
open access

Phenotypic Analysis of Medicago truncatula NPF1.7 Over-Expressing Plants Grown under Different Nitrate Conditions

Description: Plants have many nitrate transporters; in the model legume Medicago truncatula, MtNPF1.7 is among them. MtNPF1.7 is important for M. truncatula growth and it has been established that MtNPF1.7 is a high affinity nitrate transporter. M. truncatula plants with mutations in MtNPF1.7 gene show defects during plants growth, with striking abnormalities in nodule development and root architecture. Nitrogen fixation is an energy expensive process; when legumes have sufficient bioavailable nitrogen like… more
Date: December 2017
Creator: Cai, Jingya
Item Type: Refine your search to only Thesis or Dissertation
open access

Evaluating the Role of UV Exposure and Recovery Regimes in PAH Photo-Induced Toxicity to Daphina Magna

Description: Polyaromatic hydrocarbons (PAHs) are contaminants synthesized through incomplete combustion of carbon based substances. PAHs are known to be photodynamic and toxicity increases exponentially when in contact with ultraviolet radiation (UV). The effect of UV absent recovery periods and potential for latent toxicity during photo-induced toxicity are previously unknown and are not included within the toxicity model. Results of equal interval tests further support the current reciprocity model as a … more
Date: August 2017
Creator: Gnau, Jennifer Leigh
Item Type: Refine your search to only Thesis or Dissertation
open access

Neurological Responses to a Glucose Diet in Caenorhabditis elegans

Description: TRPV channels play a role in both mammalian insulin signaling, with TRPV1 expression in pancreatic beta-cells, and in C. elegans insulin-like signaling through expression of OSM-9, OCR-1, and OCR-2 in stress response pathways. In response to a glucose-supplemented diet, C. elegans are know to have sensitivity to anoxic stress, exhibit chemotaxis attraction, and display reduced egg-laying rate. Transcriptome analysis reveals that glucose stimulates nervous system activity with increased transcri… more
Date: August 2017
Creator: Dumesnil, Dennis
Item Type: Refine your search to only Thesis or Dissertation
open access

Generating Molecular Biology Tools to Investigate the Ca2+ Binding Ability of Arabidopsis TON2

Description: The position of the cell division plane in plants is determined by the position of the preprophase band. The pre prophase band (PPB) is a ring of microtubules centered around the nucleus on the inner side of plasma membrane that establishes the cortical division site. The PPB forms at the end of G2 and breaks down at the end of prophase leaving behind protein markers of its position that are collectively called the cortical division site. During cytokinesis the phragmoplast expands towards the … more
Date: August 2017
Creator: Shao, Danyang
Item Type: Refine your search to only Thesis or Dissertation
open access

Delivery of CRISPR/Cas9 RNAs into Blood Cells of Zebrafish: Potential for Genome Editing in Somatic Cells

Description: Factor VIII is a clotting factor found on the intrinsic side of the coagulation cascade. A mutation in the factor VIII gene causes the disease Hemophilia A, for which there is no cure. The most common treatment is administration of recombinant factor VIII. However, this can cause an immune response that renders the treatment ineffective in certain hemophilia patients. For this reason a new treatment, or cure, needs to be developed. Gene editing is one solution to correcting the factor VIII muta… more
Date: August 2017
Creator: Schneider, Sara Jane
Item Type: Refine your search to only Thesis or Dissertation
open access

Investigating Human Gut Microbiome in Obesity with Machine Learning Methods

Description: Obesity is a common disease among all ages that has threatened human health and has become a global concern. Gut microbiota can affect human metabolism and thus may modulate obesity. Certain mixes of gut microbiota can protect the host to be healthy or predispose the host to obesity. Modern next-generation sequencing technique allows accessing huge amount of genetic information underlying microbiota and thus provides new insights into the functionality of these micro-organisms and their interac… more
Date: August 2017
Creator: Zhong, Yuqing
Item Type: Refine your search to only Thesis or Dissertation
open access

Effect of pH on the Persistence and Toxicity of Cyfluthrin to Chironomus Tentans

Description: The effect of pH upon the aquatic toxicity of cyfluthrin was determined in 48 h static acute toxicity tests using 2nd instar Chironomus tentans larvae. Tests were conducted in both dechlorinated tap water and lake water of pH 8.0, 7.2, and 6.6. After 48 h, immobilized and dead larvae were removed and replaced with 2nd instar larvae to assess the persistence of toxicity. Midges were cultured in water adjusted to the pH values used in testing. Toxicity if cyfluthrin varied inversely with pH. An i… more
Date: May 1992
Creator: Hambleton, Faith Ann (Faith Ann Elizabeth)
Item Type: Refine your search to only Thesis or Dissertation
open access

Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course

Description: Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr G.A.O. Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiment, essays,. preparation and analysis of plasmid DNA and various o… more
Date: August 1998
Creator: Kumar, Shalini
Item Type: Refine your search to only Thesis or Dissertation
open access

A Water Quality Study of Lake Texoma

Description: A series of four stations for sampling the waters of the Red Rivers and Lake Texoma was established. Water samples from top, middle, and bottom levels of these four stations were taken on monthly intervals during periods of homothermousity and on two-week intervals during periods of thermal stratification. These samples were analysed for the cations sodium, potassium, calcium, and magnesium, and for the anions normal carbonate, bicarbonate, sulfate, chloride, nitrate, water hardness (expressed … more
Date: June 1959
Creator: Goodman, John Willis
Item Type: Refine your search to only Thesis or Dissertation
open access

Effects of Nutrient Media on Growth and Morphology of Azotobacter Vinelandii

Description: The work described in this thesis was undertaken to study the reasons why Azotobacter vinelandii ATCC 12837 after incubation in Burk's nitrogen-free liquid will not form as many colonies when plated on Difco Tryptic Soy Agar as when planted on Burk's nitrogen-free agar. The difference in growth of A. vinelandii on the two agars was established by performing viable cell-plate counts. The difference in growth was most apparent at 24-hours incubation of the Burk's liquid-media cultures. Phase cont… more
Date: August 1974
Creator: Butsch, Robert W.
Item Type: Refine your search to only Thesis or Dissertation
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A Comparison of Thermogenesis by Selected Substrates on Hypothermic Rat Liver

Description: The thermogenic effects in hypothermia of four substrates--alanine, glycine, ethano, and pyruvate - were studied in seventeen experiments. Albino rats were decapitated, and their livers were removed. The livers were homogenized with phosphate buffer at -5° C. After equilibration in a refrigerated Warburg apparatus at 20° C, the substrates were added and tissue respiration was recorded over three hours. Heat production was calculated from O2 uptake and CO2 production. Results showed that alanine… more
Date: August 1979
Creator: Long, James T.
Item Type: Refine your search to only Thesis or Dissertation
open access

Respiratory Responses in the Freshwater Snail (Pomacea Bridgesii) are Differently Affected by Temperature, Body Mass,and Oxygen Availability

Description: Pomacea bridgesii is a snail species native to tropical and sub-tropical regions, where it usually faces variability in water, temperature and oxygen level. This study of the effect of temperature on mass-specific oxygen consumption (ṀO2) and its relation to body weight shows that the ṀO2 of juvenile snails in normoxia (18-21 kPa) acclimated at temperature of 25°C ranged from 5 to 58 µMol O2/g/h, with a mean of 41.4 ± 18.3 µMol O2/g/h (n=7). Adult snails in normoxia at 25°C show less variat… more
Date: August 2016
Creator: Frifer, Wenasa Salem
Item Type: Refine your search to only Thesis or Dissertation
open access

The Role of Cysteinyl Leukotriene Receptor 2 in Thrombocyte Aggregation

Description: Cysteinyl leukotriene receptor 2, a G-protein coupled receptor known to be expressed and functional on human platelets. However, it seems that upon ligand activation the cysteinyl leukotriene receptor 2 activates a variety of signaling pathways in multiple cell types among different species. Previously, a former laboratory member Vrinda Kulkarni found cysteinyl leukotriene receptor 2 to be expressed on the surface of adult zebrafish thrombocytes. In this work I studied the characteristics of ag… more
Date: December 2015
Creator: Reyna, Julianna
Item Type: Refine your search to only Thesis or Dissertation
open access

Phenotypic Morphological Plasticity Induced by Environmental Salt Stress in the Brine Shrimp, Artemia franciscana

Description: Phenotypic plasticity is the ability of an organism to express different phenotypes in response to biotic or abiotic environmental cues. The ability of an organism to make changes during development to adjust to changes in its environment is a key to survival. Sexually reproducing organisms that have short life cycles and that are easy to raise in the laboratory are more conducive for developmental phenotypic plasticity. Considerable research has already been carried out on the brine shrimp, Ar… more
Date: December 2015
Creator: Jones, Shaun Gray
Item Type: Refine your search to only Thesis or Dissertation
open access

A High-fat Meal Alters Post-prandial mRNA Expression of SIRT1, SIRT4, and SIRT6

Description: Sirtuins (SIRT) regulate the transcription of various genes involved in the development of diet-induced obesity and chronic disease; however, it is unknown how they change acutely following a high-fat meal. The purpose of this study was to determine the effect of a high-fat meal (65% kcals/d; 85% fat recommendation), on SIRT1-7 mRNA expression in blood leukocytes at 1, 3, and 5-h post-prandial. Men and women (N=24) reported to the lab following an overnight fast (>12H). Total RNA was isolated a… more
Date: December 2015
Creator: Best Sampson, Jill Nicole
Item Type: Refine your search to only Thesis or Dissertation
open access

The Effect of Post-exercise Ethanol Consumption on the Acute Hormonal Response to Heavy Resistance Exercise in Women

Description: The purpose of this study was to examine the hormonal response to acute ethanol ingestion following a bout of heavy resistance exercise in women. Eight resistance trained women completed two identical acute heavy resistance exercise tasks (AHRET). From 10-20 minutes post-AHRET, participants consumed either a grain ethanol or a placebo beverage. Blood was collected before (PRE) and immediately after the AHRET (IP) and then every 20 minutes for five hours. Blood collected after beverage ingestion… more
Date: December 2015
Creator: Budnar, Ronald Gene, Jr.
Item Type: Refine your search to only Thesis or Dissertation
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