Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764
Description:
The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which the substrate is initially detoxified by complexation to an appropriate lig…
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Date:
August 2000
Creator:
Parab, Preeti