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open access

Aryl Hydrocarbon Hydroxylase and Sixteen Alpha Hydroxylase in Cultured Human Lymphocytes

Description: Cultured human lymphocytes may be assayed for aryl hydrocarbon hydroxylase (AHH) in whole cell preparations. The optimum assay conditions are pH 8.5, and 1.5 mM Mg++. The reaction is linear with time and cell number, and is inhibited by CO. Estradiol may inhibit induction of AHH by 3-methylcholanthrene, but is a poor competitor for the enzyme. A Caucasian population was assayed for AHH activity. The distribution was lognormal; no difference was found in cultured cells from males and females or … more
Date: December 1975
Creator: Coomes, Marguerite L.
open access

An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa

Description: The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the iso… more
Date: May 1978
Creator: Christensen, John N.
open access

The Autecology of Celtis Laevigata in Flood Plain Forests of Denton County, Texas

Description: This thesis describes the present nature of one facet of some of the flood plain forest stands in Denton County, Texas. The specific purpose was to demonstrate the presence or absence of difference between the Celtis laevigata (commonly known as the hackberry, southern hackberry or sugarberry) populations in stands on Denton Creek and Elm Fork of the Trinity River.
Date: January 1970
Creator: Hander, Lecil B.
open access

Biochemical Genetics of Certain Species of the Blackbird Family Icteridae

Description: Starch gel electrophoresis was used to compare 14 proteins encoded by 15 loci for seven species of the family Icteridae. A close genetic relationship among these species was classified into three groups. The Agelaiine group contained Agelaius phoeniceus, Sturnella magna, and S. neglecta. The Quiscaline group contained Euphagus cyanocephalus, Cassidix mexicanus, and Quiscalus quiscula. Molothrus ater, the most divergent, was placed in a separate group. Divergence times for the seven species were… more
Date: December 1974
Creator: Smith, Jackson Kelly
open access

Biochemical Genetics of the Pocket Gopher Genus Geomys, and its Phylogenetic Implications

Description: Electrophoretic techniques were utilized for the demonstration of variation in 22 proteins from 24 natural populations of four species ( G. bursarius, G. pinetis, G. arenarius and G. personatus ) of the Geomys complex of pocket gophers. Of the 24 structural loci , 19 were considered to be polymorphic. Five of the six esterases contributed greatested to the polymorphism while non-esterase proteins generally showed low values. In the GeoMys complex of pocket gophers in this study,selection appear… more
Date: December 1974
Creator: Penney, Dan F.
open access

Biological Nitrogen Fixation in Two Southwestern Reservoirs

Description: This investigation has determined the presence of biological nitrogen fixation in two reservoirs in the southwestern United States: Lake Arlington and Lake Ray Hubbard. Subsequent tests have gathered baseline data on the effects of various biological, chemical, and physical parameters on in situ nitrogen fixation in these reservoirs. Of specific importance is the relationship between nitrogen fixation arid occasional blooms of blue-green algae which produce such problems as testes and odors in … more
Date: August 1973
Creator: Lawley, Gary G.
open access

Biosystematic Study of a Desmodium Complex

Description: An examination of the Desmodium canescens complex (D. canescens; D. tweedyi; D. illinoense) has resulted in the delimitation of a previously unreported alliance between D. canescens and D. tweedyi. The following points support this view: (a) morphological data taken from herbarium and garden specimens indicate that for many characters, the mean values of D. canescens and D. tweedy are not significantly different (b) breeding experiments have shown that artificial interspecific hybridization is … more
Date: December 1977
Creator: Williams, John G., 1949-
open access

Capillary Permeability to Narrow-Range Macromolecular Dextrans at Normal and Hypobaric Pressures

Description: In view of its varied concepts and interpretations, and because of the discrepancies produced by the previous utilization of polydispersed dextrans, a study using extremely narrow-range molecular weight dextran fractions was initiated to reevaluate and consolidate some of the aspects of capillary permeability. A portion of the study was performed under decreased barometric pressure in order to clarify further some of the mechanisms involved in particulate transfer across the capillary endotheli… more
Date: December 1972
Creator: Norris, John Anthony
open access

Carbon Flux in Reservoir Sediments

Description: The central objective of the study was to fractionate sedimenting organic materials according to their source (allochthonous or autochthonous) and ultimately to determine the degree of biodegradability of contributions from either source with particular reference to activities at the mud-water interface.
Date: May 1973
Creator: Newton, Charles Eugene
open access

Chemical Composition of the Peptidoglycan of Vitreoscilla Stercoraria

Description: The peptidoglycan layer of Vitreoscilla stercoraria, ATCC 15218, was isolated from intact cells after treatment with sodium lauryl sulfate (SLS) and digestion with Pronase. Amino acid and amino sugar content was analyzed and 67% of the total present was made up of glutamic acid, alanine, diaminopimelic acid (DAP), and glucosamine in a molar ratio of 1:1.7:1:0.7. Electron microscopy of the final peptidoglycan product showed a thin, delicately folded sacculus which exhibited a morphology differen… more
Date: August 1976
Creator: Levit, Gary
open access

Chronic Acceleration and Osteogenesis

Description: The effect of excess gravity on bone-forming elements of the growing perichondrial shaft of embryonic chicks was investigated through the use of the transmission electron microscope and various cytochemical techniques.
Date: August 1973
Creator: Borgens, Richard Ben
open access

Cigarette Residues Affect Steroidogenesis in Cultured Y-1 Mouse Adrenal Tumor Cells

Description: This study (1) quantitatively compared steroid production in cultured Y-l mouse adrenal tumor cells exposed to Camel and Carlton-smoke derived residues, and (2) localized the effects in the cell. Basal steroid production was increased by Camel residues but not by Carlton, while ACTH stimulation was interfered with by both residues. Camel basal stimulation was comparable to that of cAMP, and was abolished by Cytochalasin D. The stimulation was also comparable to that of cholera toxin, which acti… more
Date: December 1979
Creator: Morris, Paula D.
open access

Colchicine Reversibly Inhibits Electrical Activity in Arthropod Mechanoreceptors

Description: Dendrites of cockroach tibial spine mechanoreceptors contain hundreds of free microtubules, which may have some relation to the generation of electrical activity. Deflection of a spine produces a train of action potentials. Continuous perfusion over a period of 4 hours results in no response decrement. Perfusion with 10mM colchicine reversibly inhibits the response within 5-7 minutes. Irreversible inhibition is produced by perfusion with 1mM vinblastine sulfate in perfusion solution containing … more
Date: August 1977
Creator: Reagan, Paul D.
open access

Comparative Chemistry of Thermally Stressed North Lake and Its Water Source, Elm Fork Trinity River

Description: To better understand abiotic dynamics in Southern reservoirs receiving heated effluents, water was analyzed before and after impoundment in 330 ha North Lake. Macronutrients, metals, and chlorinated hydrocarbons were measured. Concentrations of nutrients and metals in sediments were quantified in this 2 yr study. River water prior to impoundment contained 16 times more total phosphorus, and supported 23 times more Selenastrum capricornutum cells in an algal assay than reservoir water. The reser… more
Date: December 1976
Creator: Sams, Barry L.
open access

Comparative Ultrastructural Study between Young and Adult forms of Trypanosoma Lewisi

Description: The purpose of this study was to examine the ultrastructure of both young (dividing form) and adult (stationary form) of Trypanosoma lewisi. Changes observed between the two forms of the organism were related directly to data obtained from previous comparative biochemical studies conducted on these forms of Trypanosoma lewisi.
Date: August 1973
Creator: Abdul-Salam, Jasem M.
open access

A Comparison of Thermogenesis by Selected Substrates on Hypothermic Rat Liver

Description: The thermogenic effects in hypothermia of four substrates--alanine, glycine, ethano, and pyruvate - were studied in seventeen experiments. Albino rats were decapitated, and their livers were removed. The livers were homogenized with phosphate buffer at -5° C. After equilibration in a refrigerated Warburg apparatus at 20° C, the substrates were added and tissue respiration was recorded over three hours. Heat production was calculated from O2 uptake and CO2 production. Results showed that alanine… more
Date: August 1979
Creator: Long, James T.
open access

Differences in Protein Constituents of Some Azotobacter Species

Description: This study used polyacrylamide gel electrophoresis to study the acid-phenol soluble proteins of five strains (A. vinelandii 12837, A. vinelandii 0, A. chroococcum 8004, A. macrocytogenes 8702, A. tumefaciens) of bacteria grown on Burk's nitrogen-free media, Trypticase Soy Broth, and 0.3% butanol medium. The results showed that the protein patterns can be used for the identification and possibly the taxonomic classification of the Azotobacter. The change of phenotype of the bacteria in different… more
Date: August 1975
Creator: Hsu, Li-Chu Yao
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