UNT Libraries - 33 Matching Results

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open access

Alterations in Fatty Acid Amide Hydrolase (Faah) Transcript Levels and Activity Lead to Changes in the Abiotic Stress Susceptibility of Arabidopsis Thaliana

Description: N-Acylethanolamines (NAEs) are a class of bioactive lipids, and FAAH is one of the enzymes responsible for degrading NAEs in both plants and animals. in plants, FAAH appears to be closely associated with ABA, a phytohormone which has long been associated with plant stress responses, since the overexpression of FAAH in Arabidopsis results in ABA hypersensitivity. Therefore, it is reasonable to speculate that alterations in FAAH transcript levels will result in altered stress responses in plant… more
Date: May 2012
Creator: Gonzalez, Gabriel
Item Type: Refine your search to only Thesis or Dissertation
open access

Analysis of Mature and Young Thrombocytes in Zebrafish

Description: Eukaryotic platelets are small cell fragments that are released into the bloodstream from megakaryocytes, and their production is initiated in the bone marrow. They are mainly involved in blood hemostasis and thrombus formation. The newly synthesized platelets are called reticulated platelets or young platelets. Zebrafish thrombocytes are equivalent to mammalian platelets and have similar characteristics and functions. Likewise, zebrafish has both young and mature thrombocytes. Only young throm… more
Date: August 2018
Creator: Fallatah, Weam
Item Type: Refine your search to only Thesis or Dissertation
open access

Analysis of N-Acylethanolamines in the Oilseed Crop Camelina sativa

Description: To better understand the nature and function of N-acylethanolamines (NAEs) in Camelina sativa, we used mass spectrometry analysis to identify and quantify NAE types in developing seeds, desiccated seeds and seedlings. Developing seeds showed a differential increase in individual NAE species and an overall increase in NAE content with seed development and maturation. The NAE composition in mature, desiccated seeds mostly reflected the total fatty acid composition in the seed tissues, except fo… more
Date: August 2020
Creator: Corley, Chase D
Item Type: Refine your search to only Thesis or Dissertation
open access

brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays

Description: Subsidiary mother cell (SMC) divisions during stomatal complex formation in Zea mays are asymmetric generating a small subsidiary cell (SC) and a larger epidermal cell. Mutants with a high number of abnormally shaped subsidiary cells include the brick1 (brk1) and discordia1 (dcd1) mutants. BRK1 is homologous to HSPC300, an ARP2/3 complex activator, and is involved in actin nucleation while DCD1 is a regulatory subunit of the PP2A phosphatase needed for microtubule generation (Frank and Smith, 2… more
Date: August 2014
Creator: Malhotra, Divya
Item Type: Refine your search to only Thesis or Dissertation
open access

Cloacal Microbiota of Captive-bred and Wild Attwater’s Prairie-chicken, Tympanuchus Cupido Attwateri

Description: The Attwater’s prairie-chicken (Tympanuchus cupido attwateri; APC) is a species of grouse native to Texas coastal prairies and is on the critically endangered species list as a result of habitat destruction and overhunting. All of the current populations were captively bred and released into the wild. Survivorship for released APCs is very low, and individuals seldom survive to reproduce in the wild. One factor contributing to this may be an alteration in the gut microbiota as a result of ca… more
Date: August 2014
Creator: Simon, Stephanie E.
Item Type: Refine your search to only Thesis or Dissertation
open access

Comparison and Genetic Analysis of Host Specificity in Cluster BD1 Bacteriophages infecting Streptomyces

Description: Bacteriophages are viruses that specifically infect bacteria. When a phage infects a bacterium, it attaches itself to the surface of the bacteria and injects its DNA into the intracellular space. The phage DNA hijacks the cellular machinery of the bacteria and forces it to produce phage proteins. Eventually, the bacteria cell bursts or lyses, releasing new phage. The bacteria act as a host for phage reproduction. The ability for a phage to infect multiple bacterial species is known as host rang… more
Date: May 2019
Creator: Klug, Hannah
Item Type: Refine your search to only Thesis or Dissertation
open access

Cytochrome P450 Gene Expression Modulates Anoxia Sensitivity in Caenorhabditis Elegans

Description: With an increasing population suffering from obesity or Diabetes Mellitus (DM), it is more pertinent than ever to understand how physiological changes impact cellular processes. Patients with DM often suffer from obesity, hyperglycemia, altered fatty acids that contribute to vascular dysfunction, and increased risk to ischemia. Caenorhabditis elegans is a model system used to study the conserved insulin signaling pathway, cellular responses in whole organisms and the impact a glucose diet has o… more
Date: August 2016
Creator: Quan, Daniel L
Item Type: Refine your search to only Thesis or Dissertation
open access

Designing Tools to Probe the Calcium-dependent Function of Arabidopsis Tonneau2

Description: Plants possess unique features in many aspects of development. One of these features is seen in cell wall placement during cytokinesis, which is determined by the position of the preprophase band (PPB) and the subsequent expansion of the phragmoplast that deposits the new cell wall. During phragmoplast expansion, the phragmoplast tracks to the cortical division site, which was delineated by the PPB. Thus the position of the PPB determines the orientation of the division plane. In Arabidopsis … more
Date: December 2013
Creator: Oremade, Oladapo O.
Item Type: Refine your search to only Thesis or Dissertation

Developing a Generalizable Two-Input Genetic AND Logic Gate in Arabidopsis thaliana for Multi-Signal Processing

Description: With effective engineering using synthetic biology approaches, plant-based platforms could conceivably be designed to minimize the production costs and wastes of high-value products such as medicines, biofuels, and chemical feedstocks that would otherwise be uneconomical. Additionally, modern agricultural crops could be engineered to be more productive, resilient, or restorative in different or rapidly changing environments and climates. To achieve these complex goals, information-processing ge… more
Date: December 2022
Creator: Anderson, Charles Edgar
Item Type: Refine your search to only Thesis or Dissertation

Developing Informatics Tools and Methods Utilizing Whole Genome Sequencing and Transcription Data to Aid Gene Discovery in Medicago truncatula

Description: Research into the mechanism of symbiotic nitrogen fixation between legumes and rhizobia involves a complex interaction between the organisms, and many genes involved in this remain either uncharacterized or undiscovered. Using forward genetics, mutant plant lines are screened to find new genes without reliance on software-based gene prediction. A large population of Tnt1-mutagenized Medicago truncatula lines is used for this purpose. Herein, the aid of tools like whole genome sequencing (WGS) i… more
This item is restricted from view until January 1, 2025.
Date: December 2022
Creator: Troiani, Taylor
Item Type: Refine your search to only Thesis or Dissertation
open access

Development of a Targeted Protein Residue Analysis Approach in Archaeology

Description: Liquid chromatography-mass spectrometry (LC-MS) based proteomic methods have provided archaeologists with a powerful tool for the discovery and identification of proteins within artifacts. Traditionally, discovery-based methods have utilized a non-targeted full mass scan method in an attempt to identify all proteins present within a given sample. However, increased sensitivity is often needed to target specific proteins in order to test hypotheses. Proteins present within archaeological materia… more
Date: August 2017
Creator: Scott, Ashley
Item Type: Refine your search to only Thesis or Dissertation
open access

Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing

Description: von Willebrand factor (VWF) protein acts in the intrinsic coagulation pathway by stabilizing FVIII from proteolytic clearance and at the site of injury, by promoting the adhesion and aggregation of platelets to the exposed subendothelial wall. von Willebrand disease (VWD) results from quantitative and qualitative deficiencies in VWF protein. The variability expressivity in phenotype presentations is in partly caused by the action of modifier genes. Zebrafish has been used as hemostasis animal … more
Date: May 2017
Creator: Toffessi Tcheuyap, Vanina
Item Type: Refine your search to only Thesis or Dissertation
open access

Exploration of Genome Length, Burst Time, and Burst Size of Streptomyces griseus Bacteriophages

Description: Since phages use the host resources to replicate themselves after infection, the different sizes of the phage genome should influence the replication rate. We, therefore, hypothesized that the smaller genomes should burst the cell faster than the larger ones. As well, the shorter genomes would have greater burst sizes because they should replicate faster. Here, we obtained 16 phages of various genome length. All phages were isolated on Streptomyces griseus and available in our phage bank at the… more
Date: May 2019
Creator: Maneekul, Jindanuch
Item Type: Refine your search to only Thesis or Dissertation
open access

Exploring Caffeyl-Lignin Biosynthesis in Cleome hassleriana and Polymerization of Caffeyl Alcohol in Arabidopsis thaliana

Description: C-lignin (caffeyl-lignin) is a novel linear lignin polymer found in the seed coats of several non-crop plants, notably Vanilla planifolia (Vanilla), Jatropha Curcas (Jatropha), and Cleome hassleriana (Cleome). C-lignin has several advantages over normal G/S-lignin, found in the majority of lignocellulosic biomass, for valorization in the context of bioprocessing: less cross-linking to cell wall polysaccharides (less recalcitrant biomass), ordered linkages between monomers (homogeneous polymer),… more
Date: December 2019
Creator: Harkleroad, Aaron Djuanell
Item Type: Refine your search to only Thesis or Dissertation
open access

Forward Genetic Characterization of Medicago truncatula Tnt1 Insertion Mutants Defective in Nodule Development and Symbiotic Nitrogen Fixation

Description: Legumes are unique plants because they form special structures “nodules”, via symbiotic relationships with rhizobial bacteria present in the soil. Once rhizobia mature inside nodules, they fix atmospheric nitrogen providing a source of bioavailable nitrogen to the plant. To discover novel genetic components involved in the legume-rhizobia symbiosis by using forward genetic screening, we have isolated Medicago truncatula Tnt1 insertion mutants in the R108 ecotype, which are defective in nodule d… more
Date: May 2015
Creator: Kadel, Khem L.
Item Type: Refine your search to only Thesis or Dissertation
open access

The Generation of Recombinant Zea mays Spastin and Katanin Proteins for In Vitro Analysis

Description: Plant microtubules play essential roles in cell processes such as cell division, cell elongation, and organelle organization. Microtubules are arranged in highly dynamic and ordered arrays, but unlike animal cells, plant cells lack centrosomes. Therefore, microtubule nucleation and organization are governed by microtubule-associated proteins, including a microtubule-severing protein, katanin. Mutant analysis and in vitro characterization has shown that the highly conserved katanin is needed fo… more
Date: December 2017
Creator: Alodailah, Sattam Sonitan
Item Type: Refine your search to only Thesis or Dissertation
open access

Genetic Analysis of Medicago truncatula Plants with a Defective MtIRE Gene

Description: Leguminous plants are able to fix nitrogen by establishing a symbiotic relationship with soil dwelling bacteria, called rhizobia. The model plant Medicago truncatula forms a partnership with Sinorhizobium meliloti whereby the plant gains bioavailable nitrogen and in exchange the bacteria gains carbohydrates. This process occurs within nodules, which are structures produced on the roots of the plants within which nitrogen is fixed. M. truncatula incomplete root elongation (MtIRE) was localized t… more
Date: August 2015
Creator: Alexis, Naudin
Item Type: Refine your search to only Thesis or Dissertation
open access

Homologs of Mammalian Lysosomal Lipase in Arabidopsis and Their Roles in Lipid Droplet Dynamics

Description: Lipid droplets (LDs) are organelles with many functions in cells and numerous protein interactors facilitate their biogenesis, maintenance, and turnover. The mammalian lipase responsible for LD turnover during lipophagy, LipA, has two candidate homologs in Arabidopsis: MPL1 and LIP1. One or both of these plant homologs may function in a similar manner to mammalian LipA, providing an LD breakdown pathway. To test this hypothesis, wild type (WT) Arabidopsis plants, MPL1 over-expressing (OE) muta… more
Date: December 2017
Creator: McClinchie, Elizabeth A
Item Type: Refine your search to only Thesis or Dissertation
open access

Influence of a Human Lipodystrophy Gene Homologue on Neutral Lipid Accumulation in Arabidopsis Leaves

Description: CGI-58 is the defective gene in the human neutral lipid storage disease called Chanarin-Dorfman syndrome. This disorder causes intracellular lipid droplets to accumulate in nonadipose tissues, such as skin and blood cells. Here, disruption of the homologous CGI-58 gene in Arabidopsis thaliana resulted in the accumulation of neutral lipid droplets in mature leaves. Mass spectroscopy of isolated lipid droplets from cgi-58 loss-of-function mutants showed they contain triacylglycerols with common l… more
Date: August 2016
Creator: James, Christopher Neal
Item Type: Refine your search to only Thesis or Dissertation

Investigating the Molecular Framesworks of Phloem-Cap Fiber Development in Cotton (Gossypium hirsutum)

Description: The current study focuses on the vascular cambium and the reiterative formation of phloem fiber bundles in cotton stems. The role of the TDIF-PXY-WOX pathway was examined in regulating cambial activity and the differentiation of phloem fibers. A study was conducted to identify and characterize the cotton WOX family genes, focusing on WOX4 and WOX14, aiming to identify and analyze their phylogenetic relationships, tissue-specific expression profiles, functional roles, and metabolic consequences… more
This item is restricted from view until January 1, 2026.
Date: December 2023
Creator: Kaur, Harmanpreet
Item Type: Refine your search to only Thesis or Dissertation

Investigation of Gene Functions in the Cyanotrophic Bacterium Pseudomonas fluorescens NCIMB 11764

Description: Pseudomonas fluorescens NCIMB 11764 (Pf11764) is one of a group of bacteria known as cyanotrophs that exhibit the unique ability to grow on toxic cyanide as the sole nitrogen source. This ability has previously been genetically linked to a conserved cluster of seven genes (Nit1C), the signature gene (nitC) coding for a nitrilase enzyme. Nitrilases convert nitriles to ammonia and a carboxylic acid. Still, for the Pf11764 NitC enzyme (Nit11764), no in vivo substrate has been identified, and the… more
This item is restricted from view until June 1, 2027.
Date: May 2022
Creator: Gullapalli, Jaya Swetha
Item Type: Refine your search to only Thesis or Dissertation
open access

Isolation and Characterization of Phages Infecting Streptomyces azureus

Description: Isolating novel phages using Streptomyces azureus, which produces antibiotic thiostrepton, as a host, and characterizing the genomes may help us to find new tools that could be used to develop antibiotics in addition to contribute to the databases of phages and specifically, Streptomyces phages. Streptomyces phages Alsaber, Omar, Attoomi, Rowa, and ZamZam were isolated using during this study. They were isolated from enriched soil and sequenced by Illumina sequencing method. They were isolated … more
Date: May 2018
Creator: Sulaiman, Ahmad M.
Item Type: Refine your search to only Thesis or Dissertation
open access

Metabolic Engineering in Plants to Control Source/sink Relationship and Biomass Distribution

Description: Traditional methods like pruning and breeding have historically been used in crop production to divert photoassimilates to harvested organs, but molecular biotechnology is now poised to significantly increase yield by manipulating resource partitioning. It was hypothesized that metabolic engineering in targeted sink tissues can favor resource partitioning to increase harvest. Raffinose Family Oligosaccharides (RFOs) are naturally occurring oligosaccharides that are widespread in plants and are … more
Date: August 2013
Creator: Lahiri, Ipsita
Item Type: Refine your search to only Thesis or Dissertation
open access

Presence of Wolbachia, A Potential Biocontrol Agent: Screening for Vertebrate Blood Meal Source and West Nile Virus in Mosquitoes in the North Texas Region

Description: West Nile virus (WNV) is a geographically endemic mosquito-borne flavivirus that has spread across the United States infecting birds, mosquitos, humans, horses and other mammals. The wide spread nature of this virus is due to the ability of the mosquito vector to persist in broad, ecological diverse environments across the United States. In this study, mosquito populations in North Texas region were sampled for detection of Wolbachia, blood meal source, and WNV. The ultimate goal of this study … more
Date: August 2016
Creator: Adiji, Olubu Adeoye
Item Type: Refine your search to only Thesis or Dissertation
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