UNT Libraries - 40 Matching Results

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Analysis of the Cytochrome P450 and UDP-Glucuronosyltransferase Families and Vitamin D3- Supplementation in Anoxia Survival in Caenorhabditis elegans

Description: Alteration in diet and knockdown of detoxification genes impacts the response of C. elegans to oxygen deprivation stress. I hypothesized that feeding worms a vitamin D3-supplementation diet would result in differential oxygen deprivation stress response. We used a combination of wet lab and transcriptomics approach to investigate the effect of a vitamin-D3 supplemented diet on the global gene expression changes and the anoxia response phenotype of C. elegans (Chapter 2). C. elegans genome consi… more
Date: December 2020
Creator: Agarwal, Sujata
Item Type: Refine your search to only Thesis or Dissertation
open access

Anti-S2 Peptides and Antibodies Binding Effect on Myosin S2 and Anti-S2 Peptide's Ability to Reach the Cardiomyocytes in vivo and Interfere in Muscle Contraction

Description: The anti-S2 peptides, the stabilizer and destabilizer, were designed to target myosin sub-fragment 2 (S2) in muscle. When the peptides are coupled to a heart-targeting molecule, they can reach the cardiomyocytes and interfere with cardiac muscle contraction. Monoclonal antibodies, MF20 and MF30, are also known to interact with light meromyosin and S2 respectively. The MF30 antibody compared to anti-S2 peptides and the MF20 antibody is used as a control to test the central hypothesis that: Both … more
Date: July 2023
Creator: Quedan, Duaa Mohamad Alhaj Mahmoud
Item Type: Refine your search to only Thesis or Dissertation
open access

Compartmentalization of Jojoba Seed Lipid Metabolites

Description: Seeds from the desert shrub Simmondsia chinensis (jojoba) are one of the only known natural plant sources to store a majority of its oil in the form of liquid wax esters (WE) instead of triacylglycerols (TAGs) and these oils account for ~55% of the seed weight. Jojoba oil is highly valued as cosmetic additives and mechanical lubricants, yet despite its value much is still unknown about its neutral lipid biosynthetic pathways and lipid droplet packaging machinery. Here, we have used a multi-"omi… more
Date: December 2018
Creator: Sturtevant, Drew
Item Type: Refine your search to only Thesis or Dissertation

Defining Components Linked to Bacterial Nutritional Utilization of Cyanide as a Sole Nitrogen Source

Description: One of the challenges in biology is placing a function on the myriad of gene sequences having become available from rapid advances in genome sequencing. One such example is a gene cluster (Nit1C) found in bacteria that is tied to the unusual ability of certain bacteria to grow when supplied cyanide as the sole nitrogen source. The term cyanotrophs has been applied to such bacteria, for which a genetic linkage between cyanotrophy and Nit1C was demonstrated for 10 separate bacteria. In additio… more
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Date: May 2019
Creator: Jones, Lauren Brittany
Item Type: Refine your search to only Thesis or Dissertation
open access

The Development of Potential Therapeutic Anti-Myosin S2 Peptides that Modulate Contraction and Append to the Heart Homing Adduct Tannic Acid without Noticeable Effect on Their Functions

Description: This dissertation aimed to explore the S2 region with an attempt to modulate its elasticity in order to tune the contraction output. Two peptides, the stabilizer and destabilizer, showed high potential in modifying the S2 region at the cellular level, thus they were prepared for animal model testing. In this research, (i) S2 elasticity was studied, and the stabilizer and destabilizer peptides were built to tune contraction output through modulating S2 flexibility; (ii) the peptides were attache… more
Date: May 2021
Creator: Qadan, Motamed
Item Type: Refine your search to only Thesis or Dissertation

Effect of Phosphorus Starvation on Metabolism and Spatial Distribution of Phosphatidylcholine in Medicago truncatula Wild-Type and PDIL3 Genotypes

Description: Symbiotic nitrogen (N) fixation (SNF) occurs in specialized organs called nodules after successful interactions between legume hosts and rhizobia. Within nodule cells, N-fixing rhizobia are surrounded by plant-derived symbiosome membranes, through which the exchange of nutrients and ammonium occurs between bacteria and the host legume. Phosphorus (P) is an essential macronutrient, and N2-fixing legumes have a higher requirement for P than legumes grown on mineral N. First, I investigated the im… more
Date: August 2021
Creator: Dokwal, Dhiraj
Item Type: Refine your search to only Thesis or Dissertation

Exploring Flavonoid Glycosylation in Kudzu (Pueraria lobata)

Description: The isoflavones in kudzu roots, especially the C-glycosylated isoflavone puerarin, have been linked to many health benefits. Puerarin contains a carbon-carbon glycosidic bond that can withstand hydrolysis. The C-glycosylation reaction in the biosynthesis of puerarin has not been thoroughly investigated, with conflicting reports suggesting that it could take place on daidzein, isoliquiritigenin, or 2,7,4ʹ-trihydroxyisoflavanone. Kudzu species were identified for use in comparative transcriptomic… more
Date: August 2021
Creator: Adolfo, Laci Michelle
Item Type: Refine your search to only Thesis or Dissertation
open access

Expression of G-protein Coupled Receptors in Young and Mature Thrombocytes and Knockdown of Gpr18 in Zebrafish

Description: In this study, a novel method based on biotinylated antibodies and streptavidin coated magnetic beads was used to separate the thrombocyte subpopulations from zebrafish whole blood. DiI-C18, a lipophilic dye, labels only young thrombocytes when used at low concentrations. Commercially available biotinylated anti-Cy3 antibody was used to label the chromophore of DiI-C18 on the young thrombocytes and streptavidin coated magnetic beads were added subsequently, to separate young thrombocytes. The r… more
Date: May 2013
Creator: Potbhare, Vrinda Nikhil
Item Type: Refine your search to only Thesis or Dissertation

Fatty Acid Amide Hydrolases in Upland Cotton (Gossypium hirsutum L.) and the Legume Model Medicago truncatula

Description: Fatty acid amide hydrolase (FAAH) is a widely conserved amidase in eukaryotes, best known for inactivating the signal of N-acylethanolamine (NAE) lipid mediators. In the plant Arabidopsis thaliana, FAAH-mediated hydrolysis of NAEs has been associated with numerous biological processes. Recently, the phylogenetic distribution of FAAH into two major branches (group I and II FAAHs) across angiosperms outside of Arabidopsis (and in other Brassicaceae), suggests a previously unrecognized complexity … more
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Date: December 2023
Creator: Arias Gaguancela, Omar Paul
Item Type: Refine your search to only Thesis or Dissertation
open access

A Genetic Approach to Identify Proteins that Interact with Eukaryotic Microtubule Severing Proteins via a Yeast Two Hybrid System

Description: Microtubules (MT) are regulated by multiple categories of proteins, including proteins responsible for severing MTs that are therefore called MT-severing proteins. Studies of katanin, spastin, and fidgetin in animal systems have clarified that these proteins are MT-severing. However, studies in plants have been limited to katanin p60, and little is known about spastin or fidgetin and their function in plants. I looked at plant genomes to identify MT-severing protein homologues to clarify which … more
Date: May 2020
Creator: Alhassan, Hassan H
Item Type: Refine your search to only Thesis or Dissertation
open access

Glucose-Induced Developmental Delay is Modulated by Insulin Signaling and Exacerbated in Subsequent Glucose-Fed Generations in Caenorhabditis elegans

Description: In this study, we have used genetic, cell biological and transcriptomic methods in the nematode C. elegans as a model to examine the impact of glucose supplementation during development. We show that a glucose-supplemented diet slows the rate of developmental progression (termed "glucose-induced developmental delay" or GIDD) and induces the mitochondrial unfolded protein response (UPRmt) in wild-type animals. Mutation in the insulin receptor daf-2 confers resistance to GIDD and UPRmt in a daf-1… more
Date: December 2023
Creator: Nahar, Saifun
Item Type: Refine your search to only Thesis or Dissertation
open access

Identification and Characterization of a Mutation Causing Stunted Growth in Arabidopsis that is Linked to Phosphate Perception

Description: Plant yield is an agronomic trait dependent on the transport of photosynthate from mature source leaves to sink tissues. Manipulating phloem transport may lead to increased yield, however in a previous study, Arabidopsis thaliana overexpressing sucrose transporter AtSUC2 in the phloem resulted in stunted growth and an apparent P-deficiency. In the course of further characterizing the phenotype and identifying the causative mutation, this research included 1) reverse genetics to test genes hypot… more
Date: December 2020
Creator: Shaikh, Mearaj Ahmed A J
Item Type: Refine your search to only Thesis or Dissertation

Identification and Characterization of Genes Required for Symbiotic Nitrogen Fixation in Medicago truncatula Tnt1 Insertion Mutants

Description: In this dissertation I am using M. truncatula as a model legume that forms indeterminate nodules with rhizobia under limited nitrogen conditions. I take advantage of an M. truncatula Tnt1 mutant population that provides a useful resource to uncover and characterize novel genes. Here, I focused on several objectives. First, I carried out forward and reverse genetic screening of M. truncatula Tnt1 mutant populations to uncover novel genes involved in symbiotic nitrogen fixation. Second, I focused… more
This item is restricted from view until August 1, 2028.
Date: July 2023
Creator: Cai, Jingya
Item Type: Refine your search to only Thesis or Dissertation

Identification and Characterization of Two Putative Sulfate Transporters Essential for Symbiotic Nitrogen Fixation in Medicago truncatula

Description: The process of symbiotic nitrogen fixation (SNF) in legume root nodules requires the channeling and exchange of nutrients within and between the host plant cells and between the plant cells and their resident rhizobia. Using a forward genetics approach in the Medicago truncatula Tnt1 mutant population followed by whole genome sequencing, two putative sulfate transporter genes, MtSULTR3;5 and MtSULTR3;4b, were identified. To support the hypothesis that the defective putative sulfate transporter … more
This item is restricted from view until January 1, 2025.
Date: December 2022
Creator: Pradhan, Rajashree
Item Type: Refine your search to only Thesis or Dissertation

Identification, Characterization and Engineering of UDP-Glucuronosyltransferases for Synthesis of Flavonoid Glucuronides

Description: Flavonoids are polyphenolics compounds that constitute a major group of plant specialized metabolites, biosynthesized via the phenylpropanoid/polymalonate pathways. The resulting specialized metabolites can be due to decoration of flavonoid compounds with sugars, usually glucose, by the action of regiospecific UDP-glycosyltransferase (UGT) enzymes. In some cases, glycosylation can involve enzymatic attachment of other sugar moieties, such as glucuronic acid, galactose, rhamnose or arabinose. Th… more
Date: December 2020
Creator: Adiji, Olubu Adeoye
Item Type: Refine your search to only Thesis or Dissertation
open access

Identification of Genes Involved in Flocculation by Whole Genome Sequencing of Thauera aminoaromatica Strain MZ1T Floc-defective Mutants

Description: Thauera aminoaromatica MZ1T, a floc-forming bacterium isolated from an industrial activated sludge wastewater treatment plant, overproduces exopolysaccharide (EPS) leading to viscous bulking. This phenomenon results in poor sludge settling and dewatering during the clarification process. To identify genes responsible for bacterial flocculation, a whole genome phenotypic sequencing technique was applied. Genomic DNA of MZ1T flocculation-deficient mutants were subjected to massively parallel sequ… more
Date: December 2015
Creator: Prombutara, Pinidphon
Item Type: Refine your search to only Thesis or Dissertation
open access

Identification of Hox Genes Controlling Thrombopoiesis in Zebrafish

Description: Thrombocytes are functional equivalents of mammalian platelets and also possess megakaryocyte features. It has been shown earlier that hox genes play a role in megakaryocyte development. Our earlier microarray analysis showed five hox genes, hoxa10b, hoxb2a, hoxc5a, hoxc11b and hoxd3a, were upregulated in zebrafish thrombocytes. However, there is no comprehensive study of genome wide scan of all the hox genes playing a role in megakaryopoiesis. I first measured the expression levels of each of … more
Date: December 2015
Creator: Sundaramoorthi, Hemalatha
Item Type: Refine your search to only Thesis or Dissertation

Impact of Anti-S2 Peptides on a Variety of Muscle Myosin S2 Isoforms and Hypertrophic Cardiomyopathy Mutants Revealed by Fluorescence Resonance Energy Transfer and Gravitational Force Spectroscopy

Description: Myosin subfragment-2 (S2) is an intrinsically unstable coiled coil. This dissertation tests if the mechanical stability of myosin S2 would influence the availability of myosin S1 heads to actin thin filaments. The elevated instability in myosin S2 coiled coil could be one of the causes for hypercontractility in Familial Hypertrophic Cardiomyopathy (FHC). As hypothesized FHC mutations, namely E924K and E930del, in myosin S2 displayed an unstable myosin S2 coiled coil compared to wild type as mea… more
Date: August 2020
Creator: Aboonasrshiraz, Negar
Item Type: Refine your search to only Thesis or Dissertation
open access

Investigating Novel Streptomyces Bacteriophage Endolysins as Potential Antimicrobial Agents

Description: As antibiotic resistance has become a major global threat, the World Health Organization has urgently called scientists for alternative strategies for control of bacterial infections. Endolysin, a protein encoded by a phage gene, can degrade bacterial peptidoglycan (PG). Currently, there are three endolysin products in the clinical phase. We, thus, are interested in exploring novel endolysins from Streptomyces phages as only a few of them have been experimentally characterized. Using bioinforma… more
Date: December 2023
Creator: Maneekul, Jindanuch
Item Type: Refine your search to only Thesis or Dissertation
open access

Isolation and Genomic Characterization of 45 Novel Bacteriophages Infecting the Soil Bacterium Streptomyces griseus

Description: Bacteriophages, or simply "phages," are the most abundant biological entities on the planet and are thought to be the largest untapped reservoir of available genetic information. They are also important contributors to both soil health and nutrient recycling and have significantly influenced our current understanding of molecular biology. Bacteria in the genus Streptomyces are also known to be important contributors to soil health, as well as producing a number of useful antibiotics. The geneti… more
Date: December 2018
Creator: Hale, Richard
Item Type: Refine your search to only Thesis or Dissertation
open access

Lipogenic Proteins in Plants: Functional Homologues and Applications

Description: Although cytoplasmic lipid droplets (LDs) are the major reserves for energy-dense neutral lipids in plants, the cellular mechanisms for packaging neutral lipids into LDs remain poorly understood. To gain insights into the cellular processes of neutral lipid accumulation and compartmentalization, a necessary step forward would be to characterize functional roles of lipogenic proteins that participate in the compartmentalization of neutral lipids in plant cells. In this study, the lipogenic prote… more
Date: December 2018
Creator: Cai, Yingqi
Item Type: Refine your search to only Thesis or Dissertation

Manipulation of Lipid Droplet Biogenesis for Enhanced Lipid Storage in Arabidopsis thaliana and Nicotiana benthamiana

Description: In this study, I examined the use of mouse (Mus musculus) Fat Specific Protein 27 (FSP27) ectopically expressed in Arabidopsis thaliana and Nicotiana benthamiana as a means to increase lipid droplet (LD) presence in plant tissues. In mammalian cells, this protein induces cytoplasmic LD clustering and fusion and helps prevent breakdown of LDs contributing to the large, single LD that dominates adipocytes. When expressed in Arabidopsis thaliana and Nicotiana benthamiana, FSP27 retained its functi… more
Date: December 2021
Creator: Price, Ann Marie
Item Type: Refine your search to only Thesis or Dissertation

Medicago truncatula NPF1.7: Structure-Function Assessment and Potential as a Phytohormone Transporter

Description: In Medicago truncatula, the MtNPF1.7 transporter has been shown to be essential for root morphology and nodulation development. The allelic MtNPF1.7 mutants, Mtnip-1 (A497V), Mtnip-3 (E171K), and Mtlatd (W341STOP), show altered lateral root growth and compromised legume-rhizobium symbiosis. To assess the role of a series of distinct amino acids in the transporter's function, in silico structural predictions were combined with in planta complementation of the severely defective Mtnip-1 mutant pl… more
Date: December 2022
Creator: Yu, Yao Chuan
Item Type: Refine your search to only Thesis or Dissertation
open access

Metabolism and Action of Polyunsaturated N-acylethanolamines in Arabidopsis thaliana Seedlings

Description: The lipoxygenase (LOX) pathway plays an important role in the oxidative metabolism of polyunsaturated N-acylethanolamines (PU-NAEs). The LOX pathway functions in conjugation with hydrolysis by fatty acid amide hydrolase (FAAH) and to produce oxidized NAEs during seed germination and early seedling development. When Arabidopsis seedlings were grown in low micromolar concentrations of lauroylethanolamide (NAE 12:0), growth retardation and elevated endogenous PU-NAE levels were observed due to the… more
Date: August 2015
Creator: Keereetaweep, Jantana
Item Type: Refine your search to only Thesis or Dissertation
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