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Biochemical Identification of Molecular Components Required for Cyanide Assimilation in Pseudomonas fluorescens NCIMB 11764

Description: Utilization of cyanide as a nutritional nitrogen source in P. fluorescens NCIMB 11764 was shown to involve a novel metabolic mechanism involving nonenzymatic neutralization outside of cells prior to further enzymatic oxidation within. Several cyanide degrading enzymes were produced by NCIMB 11764 in response to growth or exposure to cyanide, but only one of these cyanide, oxygenase (CNO), was shown to be physiologically required for assimilation of cyanide as a growth substrate.
Date: May 1998
Creator: Chen, Jui-Lin
Item Type: Refine your search to only Thesis or Dissertation
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Assembly of Pseudomonas putida Aspartate Transcarbamoylase and Possible Roles of the PyrC' Polypeptide in the Folding of the Dodecameric Enzyme

Description: Aspartate transcarbamoylase (ATCase) of Pseudomonas putida consists of two different polypeptides, PyrB and PyrC' (Schurr et al, 1995). The role of the PyrC' and the assembly of PyrB and PyrC' have been studied. The ATCase made in vitro of P.putida PyrB with P.putida PyrC', and of E.coli PyrB with P.putida PyrC ' were generated under two different conditions, denaturation and renaturation, and untreated. It was found that PyrC' plays a role in the enzymatic regulation by ATP, CTP and UTP. In … more
Date: May 1999
Creator: Hongsthong, Apiradee, 1970-
Item Type: Refine your search to only Thesis or Dissertation
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Conception and Design of Constructed Wetland Systems to Treat Wastewater at the Biosphere 2 Center with Use of Reaction Rate Models and the Habitat Evaluation Procedure to Determine the Effects of Designing for Wildlife Habitat on Treatment Efficiency

Description: A study was undertaken to explore relationships between wetland characteristics which make them efficient water purifiers versus their ability to serve as wildlife habitat. The effects of designing constructed wetlands for improved habitat on water treatment efficiencies were quantified. Results indicate that some sacrifice in treatment efficiency is required and that the degree of efficiency reduction is dependant upon pollutant loading rates. However, sacrifice in efficiency is much smaller t… more
Date: May 1998
Creator: Clingenpeel, Glenn C. (Glenn Christopher)
Item Type: Refine your search to only Thesis or Dissertation
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Nucleotide Sequence Determination, Subcloning, Expression and Characterization of the xy1LT Region of the Pseudomonas putida TOL Plasmid pDK1

Description: The complete nucleotide sequence of the region encoding the DHCDH function of the pDK1 lower operon was determined. DNA analysis has shown the presence of two open reading frames, one gene consisting of 777 nucleotides encoding a polypeptide of 27.85 kDa and another gene of 303 nucleotides encoding a polypeptide of 11.13 kDa. The results of enzymatic expression studies suggest that DHCDH activity is associated only with xy1L. However although the addition of xy1T cell-free extracts to xy1L cell… more
Date: December 1992
Creator: Baker, Ronald F. (Ronald Fredrick)
Item Type: Refine your search to only Thesis or Dissertation
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Phagocytosis by Earthworm Coelomocytes : A Biomarker for Immunotoxicity of Hazardous Waste Site Soils

Description: Several biomarkers (cell viability and phagocytosis) based on earthworm (Lumbricus terrestris) immune cells (coelomocytes), together with whole-worm mortality (LC/LD50's), were used to assess a bioremediation attempt to reduce pentachlorophenol (PCP) toxicity in a former wood processing hazardous waste site (HWS).
Date: December 1997
Creator: Giggleman, Marina A.
Item Type: Refine your search to only Thesis or Dissertation
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Cell-Free Recovery and Isotopic Identification of Cyanide Degrading Enzymes from Pseudomonas Fluorescens

Description: Cell-free extracts from Pseudomonas fluorescens NCIMB 11764 catalyzed the degradation of cyanide into products that included C02, formic acid, formamide and ammonia. Cyanide-degrading activity was localized to cytosolic cell fractions and was observed at substrate concentrations as high as 100 mM. Two cyanide degrading activities were identified by: (i) the determination of reaction products stoichiometries, (ii) requirements for NADH and oxygen, and (iii) kinetic analysis. The first activity p… more
Date: December 1995
Creator: Wang, Chien-Sao
Item Type: Refine your search to only Thesis or Dissertation
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Mechanisms of Cyanide Assimilation in Pseudomonas fluorescens NCIMB 11764

Description: Pseudomonas fluorescens NCIMB 11764 was capable of utilizing cyanide as a sole nitrogen source for growth. Cyanate (OCN") and S-cyanoalanine could also serve as nitrogenous substrates, but do not appear to play a role as intermediates in cyanide metabolism. Growth of this strain on cyanate as the sole nitrogen source led to the induction of an enzyme characterized as a cyanase (EC 3.5.5.3) based on its stoichiometric conversion of cyanate to ammonia, and dependence on bicarbonate for maximal ac… more
Date: August 1992
Creator: Nagappan, Olagappan
Item Type: Refine your search to only Thesis or Dissertation
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Hooking Mortality of Largemouth Bass Caught on Controversial Artificial Lures and Live Bait : Lake Ray Roberts, Texas

Description: A total of 192 largemouth bass were caught at Lake Ray Roberts, Texas (1995) to investigate five controversial bass angling techniques relative to hooking mortality. The bait types were Texas-rigged scented and non-scented plastic worms, Carolina-rigged scented and non-scented plastic worms, and live golden shiners. Overall hooking mortality was 21.87% and mortality was dependent upon bait type. Highest mortality resulted from the Texas-rigged scented lures, while the lowest mortality was gener… more
Date: May 1996
Creator: Alumbaugh, Andrew E. (Andrew Edward)
Item Type: Refine your search to only Thesis or Dissertation
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Medial Medulla Networks in Culture: a Multichannel Electrophysiologic and Pharmacological Study

Description: Spontaneously active primary cultures obtained from dissociated embryonic medial medulla tissue were grown on microelectrode arrays for investigating burst patterns and pharmacological responses of respiratory-related neurons. Multichannel burst rates and spike production were used as primary variables for analysis. Pacemaker-like neurons were identified by continued spiking under low Ca++/high Mg++conditions. The number of pacemakers increased with time under synaptic blocking medium. Sensitiv… more
Date: August 1998
Creator: Keefer, Edward W. (Edward Wesley)
Item Type: Refine your search to only Thesis or Dissertation
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Eutrophication Monitoring and Prediction

Description: Changes in trophic status are often related to increases or decreases in the allocthonous inputs of nutrients from changes in land use and management practices. Lake and reservoir managers are continually faced with the questions of what to monitor, how to monitor it, and how much change is necessary to be considered significant. This study is a compilation of four manuscripts, addressing one of these questions, using data from six reservoirs in Texas.
Date: December 1993
Creator: Cairns, Stefan H., 1949-
Item Type: Refine your search to only Thesis or Dissertation
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Blood Pressure Regulation During Simulated Orthostatism Prior to and Following Endurance Exercise Training

Description: Cardiovascular responses and tolerance to an orthostatic stress were examined in eight men before and after eight months of endurance exercise training. Following training, maximal oxygen consumption and blood volume were increased, and resting heart rate reduced. Orthostatic tolerance was reduced following training in all eight subjects. It was concluded that prolonged endurance training decreased orthostatic tolerance and this decrease in tolerance appeared associated with attenuated barorefl… more
Date: May 1992
Creator: Stevens, Glen Harold John
Item Type: Refine your search to only Thesis or Dissertation
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Responses of Selected Texas Fishes to Abiotic Factors, and an Evaluation of the Mechanisms Controlling Thermal Tolerance of the Sheepshead Minnow

Description: Low oxygen tolerances of ten fishes were estimated using an original nitrogen cascade design, and reciprocally transformed to express responses as ventilated volume necessary to satisfy minimal oxygen demand (L·mg O2^-1). Values ranged from 0.52 to 5.64 L·mg^-1 and were partitioned into three statistically distinct groups. Eight stream fishes showed moderately high tolerances reflecting metabolic adaptations associated with stream intermittency. Juvenile longear sunfish and two mollies comprise… more
Date: May 1994
Creator: Bennett, Wayne A. (Wayne Arden)
Item Type: Refine your search to only Thesis or Dissertation
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Physical and Functional Characterization of the xy1XYZ Region From TOL Plasmid pDK1 and its Associated Downstream Regulatory Elements

Description: The nucleotide sequence for the pDKl TOL plasmid region encoding toluate-1,2-dioxygenase (Xy1XYZ, TO) was determined. TO is the first enzyme in the meta-cleavage operon, responsible for the conversion of toluates and benzoates to their carboxy-substituted diols. DNA sequence analysis revealed the presence of three open reading frames (ORF). The three ORFs correspond to xylX (1353 bp), xylY (486 bp) and xylZ (1008 bp), encoding predicted protein products of 51370 Da, 19368 Da and 36256 Da, respe… more
Date: August 1998
Creator: Hares, Douglas R. (Douglas Ryan)
Item Type: Refine your search to only Thesis or Dissertation
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Comparison of Remediation Methods in Different Hydrogeologic Settings Using Bioplume II

Description: A contaminant fate and transport computer model, Bioplume II, which allows simulation of bioremediation in ground water systems, was used to compare the effects of 11 remediation scenarios on a benzene plume. The plume was created in three different hydrogeologic settings from the simulation of an underground gasoline storage tank leak.
Date: May 1996
Creator: White, Sherry A. (Sherry Anne)
Item Type: Refine your search to only Thesis or Dissertation
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In Vitro Studies of Nuclear Changes in Mammalian CNS Neurons Subjected to Rapid Acceleration Impact Injury

Description: An in vitro model of Rapid Acceleration Impact (RAI) Injury was used to study the effects of multiple impact (220 g/impact, 3-5 sec intervals) trauma on cultures of murine CNS cells. Investigations with spinal cord cultures showed that 1) multiple impacts delivered tangential to the plane of cell growth caused neuronal death (12% after 3 impacts to 46% after 10 impacts); 2) multiple impacts delivered normal to the plane of cell growth were much less effective (8% dead after 10 impacts); 3) most… more
Date: May 1995
Creator: Wolf, Amy
Item Type: Refine your search to only Thesis or Dissertation
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Identification and Characterization of the Pyrimidine Biosynthetic Operon in Streptomyces griseus

Description: To further understand the ATCase/DHOase bifunctional complex formed in Streptomyces, the genes encoding these and other pyrimidine enzymes were identified and characterized. Polymerase chain reaction (PCR) was utilized in this effort. Primers were constructed by selecting conserved regions of pyrimidine genes from known gene and protein sequences of a wide variety of organisms. These sequences were then optimized to Streptomyces codon usage. PCR products were obtained from internal sites within… more
Date: May 1998
Creator: Hooten, Jody J. (Jody Jeran)
Item Type: Refine your search to only Thesis or Dissertation
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Biology and Energetics of Tropisternus Lateralis Nimbatus (SAY) (Coleoptera: Hydrophilidae) in a Playa on the Southern High Plains of Texas and Aquatic Coleoptera Diversity from Seven Playas on the Southern High Plains of Texas

Description: A study of the biology of Tropisternus lateralis, a hydrophilid beetle, was conducted during the flood period of a single playa on the Southern High Plains of Texas from early June 1995 through early September 1995. Mechanism of colonization, tolerance/avoidance to drought, larval density, and secondary production were analyzed. T. lateralis colonized playas from surrounding aquatic habitats and avoided drought through aerial dispersion. Once in the playa, larval density increased over time. Se… more
Date: December 1997
Creator: Cook, Robert E. (Robert Edward), 1969-
Item Type: Refine your search to only Thesis or Dissertation
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Comparison of Risk Assessment-Predicted Ecologically Safe Concentrations of Azinphos-Methyl and Fenvalerate to Observed Effects on Estuarine Organisms in a South Carolina Tidal Stream Receiving Agricultural Runoff

Description: A prospective ecological risk assessment method was developed evaluating the cumulative probabilistic impact of chemical stressors to aquatic organisms. This method was developed in response to the need to evaluate the magnitude, duration and episodic nature of chemical stressors on aquatic communities under environmental exposure scenarios. The method generates a probabilistic expression of the percent of an ecosystem's species at risk from a designated chemical exposure scenario.
Date: August 1997
Creator: Morton, Michael Gerard, 1957-
Item Type: Refine your search to only Thesis or Dissertation
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Selection and Use of Aquatic Vegetation by Migratory Waterfowl in North Central Texas

Description: Assessment of aquatic plant selection by waterfowl has been conducted during the winters of 1997-2000 on 49 0.2-0.79 ha research ponds in north central Texas. Ponds were categorized by dominant plant species into eight habitat types. Census with waterfowl species identification were performed to investigate impacts of aquatic vegetation and water depth on waterfowl. Eighteen waterfowl species were observed. Peak migration occurred in late December/early January. Mixed native ponds and mixed nat… more
Date: May 2001
Creator: Smith, JoEtta Kaye
Item Type: Refine your search to only Thesis or Dissertation
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Assessing Effects of an Environmental Education Field Science Program Fostering Responsibility at an Urban Middle School

Description: The study investigated the ability of an extracurricular program to influence environmental responsibility of sixth and seventh graders. The Children's Environmental Attitude and Knowledge Survey (CHEAKS) was evaluated for appropriateness in assessing the worth of this particular environmental education strategy emphasizing water quality fieldwork and technology. CHEAKS is designed with psychometric reliability and validity that may be used in comparing disparate programs. … more
Date: May 1999
Creator: Sills, Blake
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Prediction of Post Mortem Interval from Degradation of Endogenous Nucleotides in Human Subjects

Description: High Performance Liguid Chromatography was used to measure degradation of nucleotides in human cadavers for the purpose of prediction of post mortem interval. Endogenous nucleotides were extracted from integumentary tissue of six(6) human cadavers using six percent(6%) tricholoacetic acid. Linear regression statistical techniques were used to determine linearity of degradation of various nucleotide pools.
Date: April 1993
Creator: Williams, John Burgess
Item Type: Refine your search to only Thesis or Dissertation
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Effects of 5-hydroxytryptamine on Mouse Lumbar Motor Activity During Postnatal Development

Description: The lumbar motor activity in isolated spinal cords of 72 postnatal Balb/C mice aged 2, 5, 10 and 21 days (PN2-21) was electroneurographically recorded (ENG) via bilateral ventral roots following treatment with three different concentrations (25, 100 and 200 pM) of the neurotransmitter, 5-hydroxytryptamine (5-HT), i.e., serotonin, to determine its effects on spinal pattern generation.
Date: December 1998
Creator: Lowe-Chatham, Janice E. (Janice Elaine)
Item Type: Refine your search to only Thesis or Dissertation
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Physiological Effects of Ascaris Suum Intestinal Microflora on 5-Hydroxytryptamine Level and Binding Sites in the Intestinal Epithelial Cells

Description: Serotonin (5-hydroxytryptamine, 5-HT) has been shown to activate carbohydrate metabolism in adult female Ascaris suum. Serotonin may be either absorbed directly from the environment or synthesized de novo from the absorbed L-tryptophan in adult female A. suum. The enzymes necessary for the synthesis of 5-HT have been identified in both intestine and muscle tissues. The serotonin absorbed from the environment is obtained either from the host's gastrointestinal contents or from the 5-HT producing… more
Date: December 1991
Creator: Shahkolahi, Akbar Mohammadpour
Item Type: Refine your search to only Thesis or Dissertation
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