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open access

Carbachol- and ACPD-Induced Phosphoinositide Responses in the Developing Rat Neocortex

Description: Signal transduction via the phosphoinositide (PI) second messenger system has key roles in the development and plasticity of the neocortex. The present study localized PI responses to individual cortical layers in slices of developing rat somatosensory cortex. The acetylcholine agonist carbachol and the glutamate agonist trans-1-amino-1,3-cyclopentanedicarboxylic acid (ACPD) were used to stimulate PI turnover. The PI responses were compared to the distribution of the corresponding PI-linked rec… more
Date: August 2000
Creator: Hartgraves, Morri D.
open access

Scientific Considerations of Olestra as a Fat Substitute

Description: Olestra is, a sucrose polyester, a noncaloric fat substitute, made from sucrose and several fatty acid esters. It has been approved by the FDA as a food additive used in preparing low-fat deep-frying foods such as savory snacks. Available literature on olestra was evaluated that had both positive and negative connotations. Clinical trials in numerous species of animals including humans were conducted to determine if olestra would affect the utilization and absorption of macro- and micronutrient… more
Date: December 1999
Creator: Rattagool, Kullakan
open access

Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764

Description: The involvement of cyanide oxygenase in the metabolism of pyruvate and a-ketoglutarate-cyanohydrin was investigated and shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which the substrate is initially detoxified by complexation to an appropriate lig… more
Date: August 2000
Creator: Parab, Preeti

BioInformatics, Phylogenetics, and Aspartate Transcarbamoylase

Description: In this research, the necessity of understanding and using bioinformatics is demonstrated using the enzyme aspartate transcarbamoylase (ATCase) as the model enzyme. The first portion of this research focuses on the use of bioinformatics. A partial sequence of the pyrB gene found in Enterococcus faecalis was submitted to GenBank and was analyzed against the contiguous sequence from its own genome project. A BLAST (Basic Local Alignment Search Tool; Atschul, et al., 1990) was performed in or… more
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Date: August 2000
Creator: Cooke, Patrick Alan

Adherence and Haemagglutination of Moraxella Catarrhalis.

Description: M. catarrhalis is a gram-negative diplococci frequently associated with infections of the upper respiratory tract. During the past decade, some preliminary studies have attempted to elucidate mechanisms of adherence and haemagglutination of M. catarrhalis. These studies have reported, in many cases, inconsistent results. There are two purposes of this research. First, identify mechanisms that may potentially be associated with the adherence and haemagglutination of M. catarrhalis. Second, sug… more
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Date: August 2000
Creator: Kosterman, Edward, III
open access

Comparative Biochemistry and Evolution of Aspartate Transcarbamoylase from Diverse Bacteria

Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in pyrimidine biosynthesis. Bacterial ATCases are divided into three classes, A, B and C. Class A ATCases are largest at 450-500, are. dodecamers and represented by Pseudomonas ATCase. The overlapping pyrBC' genes encode the Pseudomonases ATCase, which is active only as a 480 kDa dodecamer and requires an inactive pyrC'-encoded DHOase for ATCase activity. ATCase has been studied in two non-pathogenic members of Mycobacteriu… more
Date: May 1999
Creator: Hooshdaran, Massoumeh Ziba
open access

A Study of the Water-Soluble Antigens from Virulent and Attenuated Biotypes of Brucella abortus

Description: Through chemical analysis and ion exchange chromatography of watersoluble antigens, this investigation supports the view that the majority of differences between the biotypes are quantitative. It was also found that strains demonstrate distinct, qualitative differences when compared to the attenuated strain 19 by immunodiffusion and thin-layer polyacrylamide gel, isoelectric focusing. These differences include the presence of antigens on virulent strains that are absent on strain 19. In additio… more
Date: May 1977
Creator: Brodeur, Richard D.
open access

Aquatic Heterotrophic Bacteria Active in the Biotransformation of Anthracene and Pentachlorophenol

Description: Dominant genera of bacteria were isolated from three river waters during anthracene and pentachlorophenol biotransformation studies. The genera Pseudomonas, Acinetobacter, Micrococcus, Chromobacterium, Alcaligenes, Azomonos, Bacillus, and Flavobacterium were capable of biotransforming one or both of these compounds. These isolates were subjected to further biotransformation tests, including river water and a basal salt medium with and without additional glucose. The results of these experiments… more
Date: August 1985
Creator: Entezami, Azam A. (Azam Alsadat)
open access

Induction of Interferon Messenger RNA and Expression of Cellular Oncogenes in Human Lymphoblastoid Cells

Description: The purposes of this study was to demonstrate the induction of alpha interferon mRNA in Sendai virus-induced Namalava cells, to follow the level of alpha interferon mRNA synthesis at the transcriptional level, and to determine whether the Namalava cell line expresses the c-myc oncogene and to what degree. The amount of c-myc message deteted in Namalva cell RNA was about one-tenth that of Daudi cell RNA, whereas no difference in the amount of the c-Ha-ras message was observed between the two cel… more
Date: December 1986
Creator: Mahmoudi, Massoud
open access

The Detection of Poliovirus in Denton Sewage by Immunofluorescence and Immunodiffusion Techniques

Description: Several final sewage effluents from the Denton Disposal Plant were demonstrated to contain Poliovirus types II and III. Pleated encapsulated filters at pH3.5 enhanced the recovery of the Poliovirus at a higher tier in comparison with nitrocellulose filter (Millipore) and glass fiber filter of pore size 0.45u. This thesis explores problems that face us today in our quest to eliminate viral pathogens from the natural and waste water needed for human, domestic, and industrial consumption. Prelimin… more
Date: August 1975
Creator: Olaiya, Felix Ayodele
open access

Immune Response of the Rat to Outer Membrane Proteins of Legionella Pneumophila

Description: Outer membrane proteins (OMPs) were recovered from eleven strains (eight serogroups) of Legionella pneumophila by sequential treatment with Tris buffer (pH 8), citrate buffer(pH 2.75) and Tris buffer (pH 8). Transmission electron microscopy revealed clearly the separation of the outer membrane from the bacteria. The development of delayed hypersensitivity was also noted by measuring the area of arythema and induration produced by intradermal injections of the MPSs from Chicago 8 strain. The adj… more
Date: August 1985
Creator: Ahanotu, Ejemihu Ndu
open access

Characterization of the Pigment-Protein and Pigment-ester of Xanthomonas Campestris Pv. Juglandis

Description: The objectives of this project were to develop a high performance liquid chromatographic method for separating the pigment esters mixture, to determine the locations of the pigment moiety in the isolated esters using pholosiphases, and to characterize the pigment-protein complex and determine its distribution in other bacteria. Saponification of the two pigment esters 1 and 2 with aqueous KOH yielded two free pigments on TLC plates developed by two solvent systems. The fasters moving of these t… more
Date: May 1987
Creator: Lawani, Leonard Olu
open access

Characterization of the Pigment-Protein Complex in Corynebacterium Poinsettiae

Description: The purpose of this study was to completely characterize the protein moiety in the caroteno complex in C. poinsettae, determine if the distribution and level of protein in the pigment-protein complex in membranes of the wild type and in a colorless mutant could account for the differences in the stability of the membrane, and to determine if this protein is common to other pigmented and non-pigmented organisms. Also, electron microscopy of cell membranes of C. poinsettiae which had been exposed… more
Date: May 1986
Creator: Ebadati, Nasrollah D.
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