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open access

The Evolutionary Genetics of Campostoma anomalum and Campostoma oligolepis

Description: Electrophoretic variation in 12 proteins encoded by 16 loci was analyzed to compare the genetic relationships of 18 natural populations representing two species of stoneroller minnows, Campostoma anomalum and C. oligolepis. Ten of the loci were monomorphic and fixed for the same allele in all populations of both species. One locus, Mdh-2, was found to separate both species. Mean heterozygosity for both species was 0.072. Estimates of levels of inbreeding indicated this phenomenon is operating i… more
Date: December 1978
Creator: Merritt, Ronald L.
open access

Pelagic Phytoplankton and Physicochemical Correlates for Lake Texoma

Description: An analysis and correlation of phytoplankton communities with physicochemical data from 3 sites in Lake Texoma was conducted to supplement time-series data. Water and phytoplankton were sampled monthly, March, 1976-February, 1977. Simple correlations were run between all physicochemical parameters and phytoplankton standing crop from the 3 sites. Multiple linear regression analyses were used to develop equations predictive of phytoplankton standing crop and chloride concentration. Minerals leac… more
Date: December 1978
Creator: McCullough, William P.
open access

Effects of Ribavirin on Normal Rat Kidney Cells and Chicken Embryo Fibroblasts Infected with Rous Sarcoma Virus

Description: Ribavirin, a synthetic nucleoside, was found to inhibit the replication of Rous sarcoma viruses (RSV) and subsequent cell transformation in chick embryo fibroblasts (CEF). It also blocked the transformation of normal rat kidney (NRK) cells infected with temperature-sensitive mutants of RSV. The action of Ribavirin was found to be reversible as removal of the drug from the NRK cells reversed the effects on cell transformation. Ribavirin appears to have a static effect on cell growth of both NRK … more
Date: April 1979
Creator: Jenkins, Frank J.
open access

Effects of an Acute Bout of Near-Maximal Intensity Exercise on the Cardiac Enzymes in Human Sera

Description: The Cardiac Profile, a pattern of serum enzyme changes seen within seventy-two hours after an AMI, is diagnostic aid for detecting occurrence of infarcts. The effects of exercise stress on the Cardiac Profile aid clinicians in avoiding diagnostic errors in patients immediately after exercise. Five male volunteers ran from six to ten miles. Serum enzyme levels were monitored serially three days before and five days after stress. Enzyme activity was determined spectrophotometrically and electroph… more
Date: May 1979
Creator: Goheen, Bernadette A.
open access

Histochemical Characterization of Lymphocytes in Preleukemic and Leukemic AKR Mice

Description: The AKR strain of mice have a genetic trait for spontaneous development of lymphocytic leukemia. In this study, leukemic mice were found to have significantly larger (p<0.01) thymuses and spleens than preleukemic mice. The enlarged leukemic tissues were densely packed with a light staining cell, with a hollow-appearing nucleus. Tissues from preleukemic mice were observed to be infiltrated with a smaller, darker-staining lymphocyte. Fluorescent antibody staining was done on preleukemic and leuke… more
Date: May 1979
Creator: Michnoff, Carolyn A.
open access

Effect of Operant Behavior on the Metabolism of 5-Hydroxytryptamine

Description: The role of operant behavior in the metabolism of brain 5-hydroxytryptamine (5-HT) turnover was investigated. Two and one-half hours following the administration of 150 mg/kg of para-chlorophenylalanine (PCPA), a specific inhibitor of tryptophan hydroxylase, levels of 5-HT were compared in sedentary and performing rats. Whole brain levels of serotonin were reduced in both responding and sedentary animals; however, differences between these groups were not statistically significant. The drug ind… more
Date: August 1979
Creator: Shepard, Paul
open access

Cigarette Residues Affect Steroidogenesis in Cultured Y-1 Mouse Adrenal Tumor Cells

Description: This study (1) quantitatively compared steroid production in cultured Y-l mouse adrenal tumor cells exposed to Camel and Carlton-smoke derived residues, and (2) localized the effects in the cell. Basal steroid production was increased by Camel residues but not by Carlton, while ACTH stimulation was interfered with by both residues. Camel basal stimulation was comparable to that of cAMP, and was abolished by Cytochalasin D. The stimulation was also comparable to that of cholera toxin, which acti… more
Date: December 1979
Creator: Morris, Paula D.
open access

Genic Differentiation Between Two Chromosomal Races of Pocket Gophers, Geomys bursarius

Description: Genic data from two chromosomal races of Geomys bursarius from a contact zone in central Texas indicated that the two races possessed distinct gene pools which would define them as separate species. Data from proteins encoded from 21 loci in this study substantiated this hypothesis. A pattern of alternately fixed alleles at the ADH-l, MDH-2, LDH-l, and IDH-1 loci with no apparent gene flow in zones of contact strongly suggested that these two races should be designated as separate species. Leve… more
Date: May 1980
Creator: Bohlin, Raymond G.
open access

Seasonal and Spatial Variability of the Microcrustacean Community in Lake Texoma, Texas and Oklahoma

Description: Twenty-eight species of zooplankton were identified from Lake Texoma. Seasonal density of the overall microcrustacean community and seasonal cycles of individual species were compared with northern populations and any available literature from the Southwest. Cycles of occurrence and abundance were similar to those observed in northern populations but tended to occur earlier in the year due to higher temperatures. Spatial distributions within the reservoir were heavily influenced by nutrient and… more
Date: August 1980
Creator: Crist, Lawrence W.
open access

The Physiology of Azotobacter Vinelandii Cysts

Description: The value of the adenylate energy charge [(ATP)+1/2(ADP)/(ATP)+(ADP)+(AMP)] in Azotobacter vinelandii cells was monitored during growth and germination in flask cultures. The miximal value of 0.88 was attained during mid-log phase; this declined gradually to 0.50 by late stationary phase. When these cultures were transferred to encystment media, the adenylate energy charge decreased to an average value of 0.40 as the vegetative cells encysted and remained unchanged during the next 20 days. Ency… more
Date: December 1980
Creator: Aladegbami, Solomon L.
open access

Taxonomy of the Azotobacteraceae

Description: The classification of the Azotobacteraceae to the level of genus and species has been uncertain since the studies of Beijerinck in 1901. This dissertation represents an effort to establish a system of classification more complete than the one now in use. In this study, both qualitative and quantitative taxonomy were used in order to establish a well founded classification scheme. Qualitative methods included certain important morphological and physiological characteristics, isoenzyme patterns, … more
Date: December 1980
Creator: Chang, Charles Shing
open access

Enhancement by Human Chorionic Gonadotropin of Transformation of Chick Embryo Fibroblasts and Rat Kidney Cells Infected with Temperature-Sensitive and Wild Type Rous Sarcoma Viruses

Description: Human chorionic gonodotropin (HCG) affected in various ways cell cultures infected with strains of Rous sarcoma virus (RSV). The cell cultures studied were chick embryo fibroblasts (CEF), normal rat kidney cells infected with temperature-sensitive mutant (LA31-NRK) and a wild type RSV (B77-NRK). HCG increased the rate of transformation and viral titer of CEF cells infected with RSV, but not B77-NRK. HCG increased significantly transformation rates of LA31-NRK, only if the temperature sensitive … more
Date: August 1981
Creator: Mitchell, Monte Mark
open access

Responses of Fishes to a Low pH Environment

Description: Data were collected from natural and introduced fishes present in Ferndale Lake, a small (120 ha) sport fishing reservoir in Camp County, east Texas. Levels of pH measured in the lake during the study period ranged from 3.5 to 5.3. Monthly field surveys and experimental manipulations were designed to evaluate quantitatively the signs of stress at various biological levels. Lethal limits to low pH were quantified for largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) to b… more
Date: August 1981
Creator: Prete, Philip J. (Philip John)
open access

Lizard Tail Autotomy: Some Aspects of its Ecology and Energetics

Description: Autotomy (self-induced loss) of body parts has evolved independently as a predator defense mechanism in several major animal phyla. The mechanism among vertebrates, including 13 of the 20 recognized lizard families, is tail autotomy. Its occurrence among the majority of lizard families offers an excellent opportunity to examine the effects of a major selective force on the ecology and evolution of a group of organisms. The research of this study was designed to examine experimentally (i) the fu… more
Date: December 1981
Creator: Dial, Benjamin Edward
open access

Microbiological Studies of Biological Activated Carbon Filters Used in Water Treatment

Description: A collaborative pilot study of the microflora on biological activated charcoal (BAC) filters employed in the tertiary treatment of drinking water revealed the principle bacterial genera to be Pseudomonas, Alcaligenes, Achromobacter, Bacillus, Micrococcus, Corynebacterium, Chromobacterium, Microcyclus and Paracoccus. The microbial population of the filters paralleled seasonal carbon dioxide production. Of particular interest were the effects of the BAC miroorganisms upon precursors of trihalomet… more
Date: December 1981
Creator: Chang, Eichin
open access

The Roles of Genic Behavioral and Biochemical Mechanisms in the Adaption of Minnows of the Genus Notropis (Cyprinidae) to Temperature

Description: Electrophoretic variation at twenty gene loci, patterns of behavioral thermoregulation, and genotype-specific malate dehydrogenase kinetics were investigated among populations of the red shiner, Notropis lutrensis, and the blacktail shiner, N. venustus, collected from thermally altered and thermally unaltered portions of their ranges. Genic variation was found to be high among red shiners and low among blacktail shiners. The behavioral response of the blacktail shiner to temperature was fixed a… more
Date: December 1981
Creator: Calhoun, Stuart W. (Stuart Wayne)
open access

Lethal and Sublethal Effects of Hemoxidants, Particularly Nitrite, on Selected Aquatic Animals

Description: A research program was developed to investigate basic and applied aspects of toxicity, both lethal and sublethal, of hemoxidants, particularly nitrite, on fish, non-fish aquatic vertebrates, and crayfish. The major objectives of this research were to determine A) acute and sublethal toxicity of nitrite to selected aquatic organisms: 1. aquatic salamander larvae, Ambystoma texanum, 2. swamp crayfish, Procambarus simulans, 3. bluegill, Lepomis macrochirus, 4. bullfrog, tadpoles, Rana catesbiana, … more
Date: May 1982
Creator: Huey, David W. (David Worley)
open access

The Effect of Intermediate Filament Inhibitors on Steroidogenesis and Cytoskeleton in Y-1 Mouse Adrenal Tumor Cells

Description: When Y-1 mouse adrenal tumor cells were treated with sodium orthovanadate, an intermediate filament (IF) inhibitor in BHK21-F cells, there was no change in the amount of 20α-dihydroprogesterone produced. A neurofilament inhibitor, β, β'-iminodipropionitrile (IDPN), enhanced the ability of Y-1 cells to produce steroid in response to ACTH by acting on the plasma membrane. Electron microscopy of Y-1 cells extracted with Triton X-100 revealed that both vanadate and IDPN caused the aggregation of cy… more
Date: August 1982
Creator: Lee, Hyun Sook
open access

Effects of Exogenous Steroids on the Adrenal Plasma Membrane Alteration of Steroidogenesis and Cell Morphology

Description: Using cultured Y-1 mouse adrenal tumor cells which produce the steroid 20(-hydroxypregn-4-en-3-one (20-DHP), it was found that 10-5 M corticosterone and deoxycorticosterone increased basal and inhibited ACTH-induced 20-DHP production. The steroid effects were concentration-dependent, reversible, and specific since six other steroids did not stimulate steroidogenesis and varied in their ability to inhibit ACTH-induced steroidogenesis. Cytochalasin D inhibited steroid-stimulated 20-DHP production… more
Date: August 1982
Creator: Mattson, Mark Paul
open access

The Growth of Azotobacter vinelandii on p-Hydroxybenzoic Acid from Soil Medium

Description: The purpose of this study was to search for the substrates utilized by Azotobacter vinelandii in dialysed soil media. Also, we sought to determine the relationship between these substrates and the growth and morphological variations of A. vinelandii. p-Hydroxybenzoic acid was shown to be used as the carbon and energy source by A. vinelandii in dialysed soil medium. The amount of this compound in the soil dialysed soil medium ranged from 14 to 21 micrograms per gram of soil. In a dialysed soil m… more
Date: August 1982
Creator: Wu, Fang Jy
open access

The Influence of Stream Regulation on Genic Differentiation and Thermal Tolerance in the Red Shiner, Notropis Lutrensis

Description: Genetic variation and thermal tolerance were surveyed for variation attributed to nonuniform selection pressures for five populations of the red shiner, Notropis lutrensis, collected from regulated and unregulated portions of a Texas river. Populations within 30 km of a hypolimnion-release dam that experience large thermal perturbations were found to have higher levels of heterozygosity, higher levels of polymorphism, significantly depressed levels of upper thermal tolerance endpoints, and grea… more
Date: December 1982
Creator: King, Timothy L. (Timothy Lee)
open access

The Effect of Human Alpha Interferon on Rat Kidney Cell Infected with Temperature-Sensitive Mutant of Rous Sarcoma Virus

Description: LA31-NRK and B77-NRK are established cell lines that were normal rat kidney cells transformed with temperature-sensitive mutant (LA31) and wild-type Bratislava 77 (B77) of Rous sarcoma virus. It is recognized that many transformation-induced changes differentiate between normal and transformed cells. Morphology and four parameters of transformed cells such as saturation density, anchorage independence, plasminogen activator, and colony stimulating factor were used as indicators to observe the e… more
Date: May 1983
Creator: Chang, Shiuan
open access

An Immunological Study of Adults with Down Syndrome

Description: The high susceptibility to infection in persons with Down Syndrome (DS) has led some investigators to explore the possibility of a defect in the immune system. Studies to date indicate no defect in humoral immunity suggesting that the defect might be in the cellular immune functions, but no specific defect has been found. Our investigation of the cellular immune system of adult DS patients was conducted by examining (1) the number and function of T-lymphocytes, (2) the phagocytic function of gr… more
Date: August 1983
Creator: White, Olivia Masih
open access

Drumming Behavior of Selected North American Stoneflies (Plecoptera)

Description: Drumming is first described for five North American stonefly species, Acroneuria evoluta, Doroneuria baumanni, Isoperla namata, Chernokrilus misnomus, and Pictetiella expansa. Signals of Acroneuria lycorias, Phasganophora capitata and Isoperla signata are further described. Drumming was not recorded from Amhinemura delosa. Signals of A. evoluta are the most complex yet recorded in Plecoptera. Doroneuria baumanni, P. expanse, C. misnomus and P. capitata have 2-way exchanges. Male D. baumanni pro… more
Date: December 1983
Creator: Maketon, Monchan
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