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  Partner: UNT Libraries
 Department: Department of Biological Sciences
 Decade: 1990-1999
 Collection: UNT Theses and Dissertations
A Multimedia Atlas of Dissection for Comparative Anatomy of the Vertebrates

A Multimedia Atlas of Dissection for Comparative Anatomy of the Vertebrates

Access: Use of this item is restricted to the UNT Community.
Date: August 1999
Creator: Curran, Anthony A., Jr.
Description: This interactive multimedia content is part of the thesis that was prepared for the degree of Master of Science in Biology: http://digital.library.unt.edu/ark:/67531/metadc2224. Traditional methods of teaching the laboratory course for Comparative Anatomy of the Vertebrates could be improved by applying current computer technology to construct an interactive, multimedial atlas of dissection. Five specimens used in comparative anatomy courses at most institutions were chosen as representative members of the Phylum Chordata: amphioxus, lamprey, dogfish shark, mud puppy, and cat. Specimens were dissected according to the modified method of Wischnitzer, 1993, and each stage was photographed with a Kodak DC120 digital zoom camera. These images were processed on a Power Macintosh 7600 computer with Adobe Photoshop v. 5.0. The atlas was constructed from these images using Macromedia Authorware v. 4.0.3. Each image contains a series of interactive objects that display a highlight and descriptive text as the cursor passes over each object.
Contributing Partner: UNT Libraries
DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers

DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers

Date: August 1997
Creator: Chiu, Angela Chen-Yen
Description: The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.
Contributing Partner: UNT Libraries
Nicotinic Acetylcholine Receptor α3 mRNA in Rat Visual System After Monocular Deprivation

Nicotinic Acetylcholine Receptor α3 mRNA in Rat Visual System After Monocular Deprivation

Date: August 1997
Creator: Taylor, James H. (James Harvey), 1970-
Description: In situ hybridization was used to examine effects of monocular enucleation on nicotinic acetylcholine receptor subunit cc3 mRNA in the rat dLGNand visual cortex. After 28 days postoperative, there were no significant differences in α3 mRNA density between the contralateral (deprived) and ipsilateral (non-deprived) sides. The lack of obvious effects of visual deprivation on α3 mRNA density suggests that other factors, possibly intrinsic to dLGNand visual cortex, govern the postnatal expression of α3 mRNA.
Contributing Partner: UNT Libraries
Nucleotide Sequence of a Bovine Arginine Transfer RNA Gene

Nucleotide Sequence of a Bovine Arginine Transfer RNA Gene

Date: May 1996
Creator: Eubanks, Aleida C. (Aleida Christine)
Description: A single plaque-pure lambda clone designated λBA84 that hybridized to a ˆ32P-labeled bovine arginine tRNA was isolated from a bovine genomic library harbored in a lambda bacteriophage vector. A 2.3-kilobase segment of this clone was found to contain an arginine transfer RNAccg gene by Southern blot hybridization analysis and dideoxyribonucleotide DNA sequencing. This gene contains the characteristic RNA polymerase III split promoter sequence found in all eukaryotic tRNAs and a potential RNA polymerase III termination site, consisting of four consecutive thymine residues, in the 3'-flanking region. Several possible cis-acting promoter elements were found within the 5'-flanking region of the sequenced gene. The function of these elements, if any, is unknown.
Contributing Partner: UNT Libraries
Subcellular Localization of N-acylphosphatidyl-ethanolamine Synthase in Cotyledons of Cotton Seedlings

Subcellular Localization of N-acylphosphatidyl-ethanolamine Synthase in Cotyledons of Cotton Seedlings

Date: December 1995
Creator: Sriparameswaran, Anuja
Description: N-acylation of phosphatidylethanolamine (PE) with free fatty acids catalyzed by N-acyl phosphatidylethanolamine (NAPE) synthase was reported in cotyledons of 24-h-old cotton seedlings. Here I report subcellular localization of this enzyme. Differential centrifugation, sucrose density gradient fractionation,aqueous two-phase partitioning and electron microscopy techniques were utilized to elucidate subcellular site(s) of NAPE synthase. Marker enzymes were used to locate organelles in subcellular fractions. Differential centrifugation indicated that NAPE synthase is present in more than one organelle and it is a membrane bound enzyme. Sucrose density gradient fractionations indicated that NAPE synthase is present in membranes derived from endoplasmic reticulum (ER),Golgi and possibly plasma membrane (PM) but not mitochondria, glyoxysomes or plastids. Aqueous two-phase partitioning experiments with cotton and spinach tissues supported these results but Goigi appeared to be the major site of NAPE synthesis. Electron microscopy of subcellular fractions was used to examine isolated fractions to provide visual confirmation of our biochemical results. Collectively, these results indicate that NAPE is synthesized in plant ER, Golgi and possibly PM.
Contributing Partner: UNT Libraries
Pyrimidine Biosynthesis in the Genus Streptomyces : Characterization of Aspartate Transcarbamoylase and Its Interaction with Other Pyrimidine Enzymes

Pyrimidine Biosynthesis in the Genus Streptomyces : Characterization of Aspartate Transcarbamoylase and Its Interaction with Other Pyrimidine Enzymes

Date: May 1998
Creator: Hughes, Lee E. (Lee Everette)
Description: Aspartate transcarbamoylase (ATCase) of Streptomyces was characterized and its interaction with other pyrimidine enzymes explored.
Contributing Partner: UNT Libraries
Upper and Lower Temperature Tolerances of Three Freshwater Game-Fish Species Exposed to Cycling Temperatures

Upper and Lower Temperature Tolerances of Three Freshwater Game-Fish Species Exposed to Cycling Temperatures

Date: August 1995
Creator: Currie, Rebecca J. (Rebecca Jean)
Description: A total of 670 critical thermal maxima (CTMax) and minima (CTMin) were determined for three freshwater fishes acclimated to three constant temperatures and a diel regimen cycling between the lowest and highest acclimation temperatures. In all species temperature tolerance was directly related to acclimation temperature and slopes relating these variables indicate that acclimation temperature has a greater influence on tolerance of low rather than high temperatures. CTMax and CTMin values generated following exposure to 32 days of oscillating temperatures indicate that in general, fishes had temperature tolerance acclimation states consistent with the average temperature and not either the highest or lowest temperature of the diel cycle.
Contributing Partner: UNT Libraries
GABAᴀ Receptors in Rat Whisker Barrel Cortex: Effects of Sensory Deprivation

GABAᴀ Receptors in Rat Whisker Barrel Cortex: Effects of Sensory Deprivation

Date: August 1995
Creator: Salazar, Eduardo, 1962-
Description: The GABAergic system in adult sensory cortex is affected by sensory deprivation, but little is known about how this predominant inhibitory system is affected during ontogeny. The present study investigates developmental effects of whisker trimming on GABAa receptors in rat barrel cortex. Rats trimmed for 6 wk beginning at birth and adulthood showed similar decreases in [3H]muscimol binding in deprived relative to non-deprived barrels, suggesting absence of a critical period.
Contributing Partner: UNT Libraries
Heat Shock Proteins in Ascaris suum

Heat Shock Proteins in Ascaris suum

Date: August 1995
Creator: Chao, Sheng-Hao
Description: Ascaris suum were exposed to a number of stressors, including heavy metals and both high (40°C) and low (18°C) temperatures. The 70kD and 90kD heat shock proteins (HSPs) in the different A. suum tissues were analyzed by Western blot and quantitated by Macintosh Image Program.
Contributing Partner: UNT Libraries
Subcloning and Nucleotide Sequence of the xylO/PUWCMA Region from the Pseudomonas putida TOL Plasmid pDK1

Subcloning and Nucleotide Sequence of the xylO/PUWCMA Region from the Pseudomonas putida TOL Plasmid pDK1

Date: December 1997
Creator: Guigneaux, Michelle M. (Michelle Marie)
Description: The TOL plasmids of Pseudomonas putida encode enzymes required for the oxidation of toluene and other related aromatic compounds. These genes are organized into two operons, the xylUWCMABN operon (upper), and the xylXYZLTEGFJQKIH operon (lower). Here we report the nucleotide sequence of a 7107 bp segment of the TOL pDK1 plasmid encoding the region just upstream of the "upper" operon through the genes encoding xylUWCMA. Sequence analysis, comparison of base-usage patterns, codon-usage patterns, and intergenic distances between genes help support the idea that the "upper" and "lower" operons have evolved independently in different genetic backgrounds and have only more recently been brought together in TOL and related catabolic plasmids.
Contributing Partner: UNT Libraries
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