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  Access Rights: Use restricted to UNT Community
  Partner: UNT Libraries
 Department: Department of Biology
 Degree Level: Master's
 Collection: UNT Theses and Dissertations
Characterization of  Moraxella bovis Aspartate Transcarbamoylase

Characterization of Moraxella bovis Aspartate Transcarbamoylase

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Date: December 2001
Creator: Hooshdaran, Sahar
Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in the pyrimidine biosynthetic pathway. Bacterial ATCases have been divided into three classes, class A, B, and C, based on their molecular weight, holoenzyme architecture, and enzyme kinetics. Moraxella bovis is a fastidious organism, the etiologic agent of infectious bovine keratoconjunctivitis (IBK). The M. bovis ATCase was purified and characterized for the first time. It is a class A enzyme with a molecular mass of 480 to 520 kDa. It has a pH optimum of 9.5 and is stable at high temperatures. The ATCase holoenzyme is inhibited by CTP > ATP > UTP. The Km for aspartate is 1.8 mM and the Vmax 1.04 ┬Ámol per min, where the Km for carbamoylphosphate is 1.05 mM and the Vmax 1.74 ┬Ámol per min.
Contributing Partner: UNT Libraries
General Biology Lecture and Laboratory Curriculum Outline in a Two or Four-Year College

General Biology Lecture and Laboratory Curriculum Outline in a Two or Four-Year College

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Date: August 2000
Creator: Moreland, Amy L.
Description: In July of 1999, I wrote to 24 Texas junior and community colleges (and one four-year institution) describing my thesis agenda of a general biology lecture and laboratory syllabus for introductory biology students. I requested the titles and authors of the general biology textbooks and laboratory manuals they were currently using, the publishers of these texts, and the edition of said texts. I then contacted publishers of the various textbooks who, in turn, directed me to the Dallas-area representatives for further inquiries. I assimilated the various authors' general biology topics into a two-semester syllabus of lecture and one semester of laboratory. The document is not a text manuscript, but an all-inclusive listing of a general biology syllabus broken down by subject.
Contributing Partner: UNT Libraries
Integrating Concepts in Modern Molecular Biology into a High School Biology Curriculum

Integrating Concepts in Modern Molecular Biology into a High School Biology Curriculum

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Date: August 2003
Creator: Parker, Timothy P.
Description: More so than any other science in the past several decades, Biology has seen an explosion of new information and monumental discoveries that have had a profound impact on much more than the science itself. Much of this has occurred at the molecular level. Many of these modern concepts, ideas, and technologies, as well as their historical context, can be easily understood and appreciated at the high school level. Moreover, it is argued here that the integration of this is critical for making biology relevant as a modern science. A contemporary high school biology curriculum should adequately reflect this newly acquired knowledge and how it has already has already begun to revolutionize medicine, agriculture, and the study of biology itself. This curriculum provides teachers with a detailed framework for integrating molecular biology into a high school biology curriculum. It is not intended to represent the curriculum for an entire academic year, but should be considered a significant component. In addition to examining key concepts and discoveries, it examines modern molecular techniques, their applications, and their relevance to science and beyond. It also provides several recommended labs and helpful protocols.
Contributing Partner: UNT Libraries
A Physiological Age-Grading System for Female Hydrellia pakistanae  Deonier (Diptera: Ephydridae)

A Physiological Age-Grading System for Female Hydrellia pakistanae Deonier (Diptera: Ephydridae)

Access: Use of this item is restricted to the UNT Community.
Date: December 2002
Creator: Lenz, Jennifer Marie
Description: Conflicting opinions about the effectiveness of H. pakistanae as a biological control agent for hydrilla prompt researchers to find a method for assessing the fly's success. Developing a physiological age-grading system for the fly using ovarian morphology to detect changes in reproductive activity is useful for evaluating reproductive status of the fly in field populations. Changes in the appearance of follicular relics in ovaries with oviposition provide a reliable method to estimate fecundity. Characteristics of follicular relics were used to develop a system with eight physiological age classes, three nulliparous and five parous. Changes that occur in the fat body were used to assist in classification of nulliparous females or those with low egg counts.
Contributing Partner: UNT Libraries