Date: April 15, 2010
Creator: Manley, Coreen M. & Hughes, Lee E.
Description: This presentation discusses research on the purification and analysis of mycobacteriophage Alice. The purpose of this research is to expand the knowledge of mycobacteriophage and analyze a single genome to be archived for future use. Mycobacteriophages are found in soil almost anywhere with an abundance of the host mycobacterial cells. The analysis of mycobacteriophage may lead to breakthroughs in DNA recombination and help the scientific community in the search for a cure for genetic diseases. In this study, a single unique phage, Alice, was isolated from a soil sample found in a compost pile. The sample was directly plated using M. smegmatis as the host organism. After the plaques were verified to be formed by phage, a series of dilutions to isolate and purify the phage were performed. The final product from the dilutions was a high titer lysate that, when prepared, was used to extract the DNA from the phage, which was then sequenced and analyzed.
Contributing Partner: UNT Honors College