Date: April 14, 2011
Creator: Harvey, Erin; Hughes, Lee E.; Benjamin, Robert C. & Simon, Stephanie E.
Description: This paper discusses research on mycobacteriophage (phage) timshel. Abstract: The purpose of this research is to isolate, purify and sequence a unique phage in order to increase the knowledge of phage. The study began by isolating a unique phage from a soil sample. The phage was purified by a series of plaque picking and plating. Once a pure phage was obtained, DNA was extracted from a high titer lysate and a restriction fragment analysis was performed to verify the uniqueness of the phage, provide a preliminary estimate of genome size, and assess of phage family. Timshel has been classified as an A4 phage based upon the electron microscopy, gel electrophoresis, and plaque morphology. This classification has recently been verified by preliminary sequence homology analyses. The sequence also reveals the phage chromosome to be linear, 53.49 kb pairs in length, to have a 10 bp sticky end, and to encode approximately 80 predicted genes.
Contributing Partner: UNT Honors College