UNT Theses and Dissertations - 7 Matching Results

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Protein Kinase C Activation in Hyperglycemic Bovine Lens Epithelial Cells

Description: This study demonstrates the presence of protein kinase C activity in both cytosolic and membrane fractions of bovine lens epithelial cells in culture. Protein kinase C activity is similar in normal and hyperglycemic cells. Furthermore, the ability of the enzyme to translocate from the cytosol to the membrane following phorbol ester treatment is unimpeded by hyperglycemic conditions. Moreover, protein kinase C activation had no effect on myoinositol uptake either in normal cells or in cells expo… more
Date: December 1993
Creator: Fan, Wen-Lin
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Studies on ADP-Ribose Polymer Metabolism in Cultured Mammalian Cells Following DNA Damage

Description: ADP-ribose polymer metabolism has been studied in human cells derived from a patient with Glutamyl Ribose Phosphate Storage Disease (GRPSD) and in mouse C3H1OT1/2 cells following oxidative stress induced by hydrogen peroxide (H202 ). It has been postulated that GRPSD resulted from an abnormality in ADP-ribose polymer metabolism. This study has shown that these cells exhibit reduced poly(ADP ribose) polymerase activity which is proposed to result from modification of the enzyme with ribose phosp… more
Date: May 1991
Creator: Maharaj, Geeta
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Characterization of Human Glucose-6-Phosphate Isomerase of Different Sizes

Description: Glucose phosphate isomerase (GPI) was purified from human placenta utilizing cross-linked spherical particle phosphocellulose. In three steps, GPI could be purified approximately 5500 fold with greater than 50% recovery. The purified enzyme exhibited four bands upon non-denaturing PAGE and isoelectric focusing (IEF) when stained with GPI specific activity stain. The four isozymes were isolated by preparative IEF. The isoelectric points of the isozymes were determined. Sodium dodecyl sulfate (SD… more
Date: December 1989
Creator: Sun, An Qiang
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Physical Mapping of Human Transfer RNA Gene Clusters

Description: Two plaque-pure phage lambda clones designated as λhtX-l and λhtX-2 that hybridized to unfractionated bovine liver tRNA were isolated from a human X chromosome-specific library. The λDNAs were characterized by restriction mapping and Southern blot hybridization techniques. The human DNA segment in λhtX-l contains five or more presumptive tRNA genes and at least one Alu family member. The 19-kilobase human DNA insert in λhtX-2 contains two or more presumptive tRNA genes and at least three Alu fa… more
Date: December 1989
Creator: Wang, Luping
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Structural Analyses of a Human Valine Transfer RNA Gene and of a Transfer RNA Pseudogene Cluster

Description: Two different cloned human DNA segments encompassing transfer RNA gene and pseudogene clusters have been isolated from a human gene library harbored in bacteriophage lambda Charon 4-A. One clone (designated as λhVal7) encompassing a 20.5-kilobase (Kb) human DNA insert was found to contain a valine transfer RNA_AAC gene and several Alu-like elements by Southern blot hybridization analysis and DNA sequencing with the dideoxyribonucleotide chain-termination method in the bacteriophage M13mp19 vect… more
Date: December 1987
Creator: Lee, Mike Ming-Jen
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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pH Dependence of the Kinetic Parameters for the Oxalacetate Decarboxylation and Pyruvate Reduction Reactions Catalyzed by Malic Enzyme

Description: Ascaris suum NAD-malic enzyme catalyzes the decarboxylation of oxalacetate and reduction of pyruvate. Thus, the present classification (E.C. 1.1.1.39) for this enzyme should be changed to E.C. 1.1.1.38. In the absence of nucleotide, both the chicken liver NADP-malic enzyme and Ascaris suum NAD-malic enzymes catalyze the decarboxylation of oxalacetate. A study of the pH dependence of kinetic parameters for oxalacetate decarboxylation and pyruvate reduction was carried out for the NAD(P)-malic en… more
Date: August 1985
Creator: Park, Sang-Hoon
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Isolation and Partial Characterization of Lecithin Cholesterol Acyltransferase and High Density Lipoprotein from Hog Plasma

Description: Lecithin:cholesterol acyltransferase (LCAT) was purified 30,000-fold from hog plasma in a homogeneous state as indicated by polyacrylamide gel electrophoresis. The purified enzyme had an apparent molecular weight of 66,000 and was found to contain about 21.4 percent (w/w) carbohydrate. The properties of hog LCAT including amino acid composition were compared with human LCAT. High density lipoprotein (HDL) was isolated from the hog plasma by an immunoaffinity column chromatography. The isolated … more
Date: May 1984
Creator: Park, Yong Bok
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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