UNT Theses and Dissertations - 4 Matching Results

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Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit

Description: A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and my… more
Date: May 2007
Creator: Coffee Castro-Zena, Pilar G.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

FLP-mediated conditional loss of an essential gene to facilitate complementation assays

Description: Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious and time-consuming step, the newly developed two-component system utilizes a site-specific recombinase to excise a wild-type copy of the gene of interest from transformed tissues. In the first componen… more
Date: December 2007
Creator: Ganesan, Savita
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft.

Description: In order to understand the structural changes in myosin S1, fluorescence polarization and computational dynamics simulations were used. Dynamics simulations on the S1 motor domain indicated that significant flexibility was present throughout the molecular model. The constrained opening versus closing of the 50 kDa cleft appeared to induce opposite directions of movement in the lever arm. A sequence called the "strut" which traverses the 50 kDa cleft and may play an important role in positioning… more
Date: August 2007
Creator: Gawalapu, Ravi Kumar
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Gene Expression Profiling of the nip Mutant in Medicago truncatula

Description: The study of root nodule symbiosis between nitrogen-fixing bacteria and leguminous plant species is important because of the ability to supplement fixed nitrogen fertilizers and increase plant growth in poor soils. Our group has isolated a mutant called nip in the model legume Medicago truncatula that is defective in nodule symbiosis. The nip mutant (numerous infections with polyphenolics) becomes infected by Sinorhizobium meliloti but then accumulates polyphenolic defense compounds in the no… more
Date: August 2007
Creator: McKethan, Brandon Lee
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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