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Effects of Gold Sodium Thiomalate on Murine Spleen Cells

Description: The effects of gold sodium thiomalate (GST) on murine spleen cells were investigated using in vitro mitogen blastogenesis techniques. Addition of GST to intact spleen cells resulted in a decreased blastogenic response to the T cell mitogen, concanavalin A (Con A). Thymidine uptake of spleen cells depleted of macrophages and cultured with Con A and GST demonstrated biphasic effects. At 2.5 pg Con A/ml, blastogenesis of macrophage depleted spleen cells was inhibited to a lesser degree than intact… more
Date: December 1986
Creator: Brownback, Paul (Paul Eldon)
Partner: UNT Libraries
open access

Internal Radiolabeling of Mycobacterial Antigens and Use in Macrophage Processing Studies

Description: Mycobacter avium complex serovars 4 and 20 were cultured in the presence of [3H] fucose, [3H]-methionine, and [3H]-mannose to specifically radiolabel the oligosaccharide of the glycopeptidolipid (GPL) antigens. Distribution of radioactivity in lipid was determined by thin-layer chromatographic methods. Examination of acid hydrolysates from radiolabeled antigens revealed that [3H]-methionine incorporated into methylated sugars in polar and apolar GPL components, whereas [3H]-mannose incorporated… more
Date: August 1988
Creator: Woodbury, Julie L. (Julie Lynn)
Partner: UNT Libraries
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