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open access

DNA-DNA Hybridization of Methane Oxidizing Bacteria

Description: Bacteria classified in the family Methylomonadaceae must derive their carbon from one-carbon compounds. They are characterized by the possession of internal membranes of two types. Type I membranes are layered and fill the middle of the cells while type II membranes form concentric layers around the periphery of the cells. Also, there are two metabolic pathways by which the methylobacteria assimilate one-carbon compounds. Further evidence of this dichotomy was sought by DNA-DNA saturation hybri… more
Date: December 1976
Creator: Ackerson, Jill W.
Partner: UNT Libraries
open access

Solvent Effects and Bioconcentration Patterns of Antimicrobial Compounds in Wetland Plants

Description: This study looked at effects of organic solvents dimethylsulfoxide, dimethylformamide and acetone at 0.01%, 0.05% and 0.1% concentration on germination and seedling development wetland plants. Even at 0.01% level, all solvents affected some aspect of seed germination or seedling growth. Acetone at 0.01% was least toxic. Root morphological characteristics were most sensitive compared to shoot morphological characteristics. This study also looked at bioconcentration patterns of antimicrobial comp… more
Date: May 2011
Creator: Adhikari, Sajag
Partner: UNT Libraries
open access

Degradation of Phenolic Acids by Azotobacter Species Isolated from Sorghum Fields

Description: Sorghum plants excrete phenolic acids which reduce subsequent crop yields. These acids accumulate in field soil by combining with soil and clay particles to form stable complexes which remain until degraded by bacterial metabolism. The amount of phenolic acids in soil samples were obtained by gas chromatography measurements, while Azotobacter populations were obtained by plate counts in 40 sorghum field samples from Denton County, Texas. One can conclude that increasing the Azotobacter populati… more
Date: August 1989
Creator: Al-Hadhrami, Mohamed N. (Mohamed Nasser)
Partner: UNT Libraries
open access

Evaluation of Diet, Water, and Culture Size for Ceriodaphnia Dubia Laboratory Culturing

Description: Six reagent waters, eleven diets, and two culture sizes were evaluated for culturing C. dubia. Different filtration techniques were used to prepare the reagent waters. The eleven diets were comprised of two algae augmented with eight supplements. Reproduction and growth were assessed to discern differences among C. dubia raised in mass cultures and cultured in individual cups, during which, bacterial population densities, lipid, protein, and carbohydrate concentrations of the diets were measure… more
Date: December 1992
Creator: Allen, Jerry D. (Jerry Dee)
Partner: UNT Libraries
open access

Retinoic acid Treatment Affects Kidney Development and Osmoregulatory System in the Developing Chicken (Gallus Gallus)

Description: Development is a dynamic process characterized by critical periods in which organ systems are sensitive to changes in the surrounding environment. In the current study, critical windows of embryonic growth and kidney development were assessed in the embryonic chicken. All‐trans retinoic acid (tRA) influences not only organogenesis and cell proliferation, but also targets metanephric kidney nephrogenesis. Embryonic chickens were given a single injection of tRA on embryonic day 8. tRA decre… more
Date: May 2011
Creator: Alvine, Travis Douglas
Partner: UNT Libraries
open access

Isolation and Bioinformatic Characterization of Four Novel Bacteriophages from Streptomyces toxytricini

Description: Six initial phage isolates with high titer lysates were obtained using Streptomyces toxytricini B-5426 as the host bacterium. These isolates were named Goby, Toma, Yosif, Yara, Deema, and Hsoos. However, upon completion of the sequencing, it was found that the Yara and Hsoos isolates were identical, as were Goby and Deema. As a result, final analysis was completed on only the four unique isolates. All of the phages mentioned above were isolated from soil samples from different locations. Al… more
Date: May 2019
Creator: Alzaid, Hessah
Partner: UNT Libraries
open access

The Effects of Probiotics on Growth, and Metabolism in Juvenile Oreochromis mossambicus (Mozambique Tilapia)

Description: Improving growth, lowering mortality rates, and having a faster turnaround to harvest is essential for the future of commercial aquaculture. The primary goal of this study was to determine if introducing a single strain probiotic Lactobacillus rhamnosus IMC 501 into the feed regimen of a commercially important aquaculture freshwater fish, Mozambique tilapia (Oreochromis mossambicus), would decrease mortality; change metabolic rates; and increase tissue wet mass (MW), standard length, growth rat… more
Date: May 2020
Creator: Anderson, Michael Earl
Partner: UNT Libraries

Evaluation of virulence in wild type and pyrimidine auxotrophs of Pseudomonas aeruginosa using the eukaryotic model system Caenorhabditis elegans.

Description: The human opportunistic pathogen, Pseudomonas aeruginosa PAO1, has been shown to kill the nematode Caenorhabditis elegans. C. elegans has been a valuable model for the study of bacterial pathogenesis, and has reinforced the notion that common virulence and host defense mechanisms exist. Recently, the pyrimidine pathway was shown to regulate virulence levels. Therefore, mutations in the pyrimidine pathway of PAO1 showed decrease virulence in the nematode. When starving the nematode, bacterial… more
Access: Restricted to the UNT Community Members at a UNT Libraries Location.
Date: August 2004
Creator: Anvari, Sara
Partner: UNT Libraries

Inquiry-based science for high school students: a forensic unit

Description: This project constitutes an instructional unit for honors biology that involves the use of science in the field of criminal investigation and forensics. Before beginning the unit, the learners should have mastered basic laboratory skills, including use of the microscope. They should also have an understanding of the basic structure and function of DNA and its role in heredity and protein synthesis. The standard time frame is 24 days with 70-minute periods, but can be easily adjusted to meet cla… more
Access: Restricted to UNT Community Members. Login required if off-campus.
Date: August 2000
Creator: Apple, Kendra Kea
Partner: UNT Libraries
open access

Effector Response of the Aspartate Transcarbamoylase From Wild Type Pseudomonas Putida and a Mutant with 11 Amino Acids Deleted at the N-terminus of PyrB.

Description: Like its enteric counterpart, aspartate transcarbamoylase (ATCase) from Pseudomonas putida is a dodecamer of two different polypeptides. Unlike the enterics, the Pseudomonas ATCase lacks regulatory polypeptides but employs instead inactive dihydroorotases for an active dodecamer. Previous work showed that PyrB contains not only the active site but also the effector binding sites for ATP, UTP and CTP at its N-terminus. In this work, 11 amino acids were deleted from the N-terminus of PyrB and t… more
Date: May 2002
Creator: Asfour, Hani
Partner: UNT Libraries
open access

Hypoxia-Induced Cardiac Arrest Alters Central Nervous System Concentrations of the GLYT2 Glycine Transporter in Zebrafish (Danio rerio)

Description: Hypoxia as a stressor has physiological implications that have been a focal point for many physiological studies in recent years. In some studies, hypoxia had large effects on the organ tissue degeneration, which ultimately effects multiple ecological processes. These organ tissue studies played a part in the development of new fields like neurocardiology, a specialty that studied the relationship between the brain and the heart. This thesis focuses on how hypoxia-induced cardiac arrest alte… more
Date: July 2023
Creator: Auzenne, Alexis
Partner: UNT Libraries
open access

Comparison of Aspartate Transcarbamoylase and Pyrimidine Salvage in Sporosarcina urea, Sprolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus

Description: The enzyme that catalyzes the committed step in pyrimidine biosynthesis, aspartate transcarbamoylase, has been compared in selected endospore-forming organisms and in morphologically similar control organisms. The ATCases and pyrimidine salvage from Sporosarcina ureae, Sporolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus were compared to those of Bacillus subtilis. While the ATCases from Sporosarcina ureae, Sporolactobacillus inulinus, and L. fermentum were found to exhi… more
Date: August 1994
Creator: Barron, Vincent N. (Vincent Neal)
Partner: UNT Libraries
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Evaluating Fish Impingement and Entrainment at the Comanche Peak Steam Electric Station

Description: This study was designed to determine if impingement and entrainment by cooling water intake at the Comanche Peak Steam Electric Station have an adverse impact upon the Squaw Creek Reservoir fish population. The yearly impingement of fish was estimated to be 262,994 of 14 species. The threadfin shad (Dorosoma petenense) accounted for 96% of this total. Entrainment of eggs and larvae for a five month period was estimated to be 15,989,987 and 42,448,794 respectively. Two fish population studies we… more
Date: May 1996
Creator: Bauml, George A. (George Arthur)
Partner: UNT Libraries

A morphological study of the avian (Gallus domesticus) ductus arteriosi during hatching.

Description: The ductus arteriosi (DA) are two blood vessels connecting the pulmonary arteries to the descending aorta in the avian embryo. Following hatching, the DA closes, separation of the systemic and pulmonary circulation. I present the morphological changes that occur in the chicken DA during prepipping, internal pipping, external pipping, and hatching. The avian DA consists of two distinct tissue types, a proximal and a distal portion. Histological examination shows developmental differences bet… more
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Date: May 2006
Creator: Belanger, Candace
Partner: UNT Libraries

Evidentiary Value of Condoms: Comparison of Durable Physical and Chemical Characteristics of Condoms

Description: Condom trace evidence must not be overlooked in sexual assault cases; understanding the chemical and physical characteristics of condoms is imperative if condoms are to be useful evidence. Previous research shows that condom identification is possible, but it is equally important to evaluate durability of condom residues versus time. Using FT-IR, this study examined vaginal swabs from subjects who self-sampled at intervals for up to 72 hours after having intercourse with a condom. This study in… more
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Date: May 2000
Creator: Belcher, Kelly Leigh
Partner: UNT Libraries
open access

Production and Energy Metabolism in Three Benthic Insect Populations in a Small North Central Texas Pond

Description: Annual energy budgets of dominant benthic macro-invertebrates were examined during November 1973 to October 1974 from the benthos of a small pond ecosystem in north-central Texas. Estimates of annual secondary production (Hynes and Coleman 1968) were Procladius s. (Diptera, Chironimidae), 2.4 g m^-2 y^-1 (13 kcal m^-2 y^-1 ); Tendipes decorus (Diptera, Chironomidae), 6.0 g m^-2 y^-1 (40 kcal m^-2 y^-1 ); Brachycercus sp. (Ephemeroptera, Caenidae), 1.9 g m^-2 y^-1 (11 kcal m^-2 y^-1). Energy met… more
Date: May 1978
Creator: Benson, Daniel J.
Partner: UNT Libraries
open access

A High-fat Meal Alters Post-prandial mRNA Expression of SIRT1, SIRT4, and SIRT6

Description: Sirtuins (SIRT) regulate the transcription of various genes involved in the development of diet-induced obesity and chronic disease; however, it is unknown how they change acutely following a high-fat meal. The purpose of this study was to determine the effect of a high-fat meal (65% kcals/d; 85% fat recommendation), on SIRT1-7 mRNA expression in blood leukocytes at 1, 3, and 5-h post-prandial. Men and women (N=24) reported to the lab following an overnight fast (>12H). Total RNA was isolated a… more
Date: December 2015
Creator: Best Sampson, Jill Nicole
Partner: UNT Libraries
open access

Documentation of Biodiversity Impacts (Including Cumulative Biodiversity Impacts) in Environmental Impact Statements

Description: In the United States, biodiversity impact assessment has historically received little attention. Responding in 1993, the Council on Environmental Quality (CEQ) released guidelines on incorporating biodiversity into environmental impact assessment under the National Environmental Policy Act of 1969. The objectives of the study here were to identify the level of documentation of biodiversity impact assessment in sample Environmental Impact Statements (EISs); identify whether in the years followin… more
Date: August 1998
Creator: Bhatia, Sarika
Partner: UNT Libraries
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Developmental Patterns of Metabolism and Hematology in the Late Stage Chicken Embryo (Gallus Domesticus) at Two Incubation Temperatures.

Description: How temperature affects physiological development in the chicken embryo is unknown. Embryos incubated at 38°C or 35°C showed no difference in growth or survival. The time to hatching was longer in 35°C than 38°C embryos (23.7 vs. 20.6 days), but unaffected was the relative timing of appearance of developmental landmarks (internal, external pipping). At stage 43-44, 38°C embryos maintained oxygen consumption around 1 mL/g/h despite acute temperature reduction (suggesting thermoregulatory maturat… more
Date: May 2003
Creator: Black, Juli
Partner: UNT Libraries
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College Freshman Biology Two Semester Course: Integrating Deep Processing Teaching Techniques

Description: Development of a college level freshman biology course was undertaken in response to government reports that American students have fallen behind students of other countries in the area of the sciences. Teaching strategies were investigated to accomplish two objectives, to define essential academic material to include in the course and to investigate teaching techniques that would increase deep processing of the information. An active process that consisted of applying the cognitive information… more
Date: May 2002
Creator: Blevins, Mary Jean
Partner: UNT Libraries
open access

Genetic Differentiation of the Geomys Pocket Gopher Complex of Texas

Description: Genetic variation was analyzed for populations of seven taxa comprising four cytotypes of the Geomys bursarius chromosome complex, including G. b. major, G. b. knoxjonesi, and the Edwards Plateau taxa, G. b. llanensis and G. b. texensis. Genetic relationships of the Edwards Plateau gophers with other taxa and between themselves were examined. Genetic similarity, number of fixed allelic differences, and ectoparasite distribution indicate the Edwards Plateau gophers are a distinct gene pool. Isol… more
Date: August 1988
Creator: Block, Scott B. (Scott Bishop)
Partner: UNT Libraries
open access

Molecular systematics of Baird's pocket gopher (Geomys breviceps)

Description: Baird's pocket gopher (Geomys breviceps) is found in eastern Texas, eastern Oklahoma, central and western Arkansas, and western Louisiana. The cytochrome-b gene was sequenced and analyzed for 16 pocket gophers from throughout the range of the species. Similar phylogenetic trees were obtained using maximum-parsimony, maximum-likelihood, neighbor-joining, and Bayesian analyses. Two major clades were formed with northern individuals belonging to clade I and southern individuals belonging to clade … more
Date: August 2010
Creator: Bodine, Deanna Martinez
Partner: UNT Libraries
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Genic Differentiation Between Two Chromosomal Races of Pocket Gophers, Geomys bursarius

Description: Genic data from two chromosomal races of Geomys bursarius from a contact zone in central Texas indicated that the two races possessed distinct gene pools which would define them as separate species. Data from proteins encoded from 21 loci in this study substantiated this hypothesis. A pattern of alternately fixed alleles at the ADH-l, MDH-2, LDH-l, and IDH-1 loci with no apparent gene flow in zones of contact strongly suggested that these two races should be designated as separate species. Leve… more
Date: May 1980
Creator: Bohlin, Raymond G.
Partner: UNT Libraries
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