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 Degree Discipline: Environmental Science
 Collection: UNT Theses and Dissertations
Photoinduced Toxicity in Early Lifestage Fiddler Crab (Uca Longisignalis) Following Exposure to Deepwater Horizon Spill Oil

Photoinduced Toxicity in Early Lifestage Fiddler Crab (Uca Longisignalis) Following Exposure to Deepwater Horizon Spill Oil

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Date: December 2015
Creator: Taylor, Leigh M.
Description: The 2010 Deepwater Horizon (DWH) oil spill resulted in a large release of polycyclic aromatic hydrocarbons (PAH) into the Gulf of Mexico. PAH can interact with ultraviolet radiation (UV) resulting in increased toxicity, particularly to early lifestage organisms. The goal of this research was to determine the sensitivity of fiddler crab larvae (Uca longisignalis) to photo-induced toxicity following exposure to Deepwater Horizon spill oil in support of the DWH Natural Resource Damage Assessment. Five replicate dishes each containing 20 larvae, were exposed to one of three UV treatments (10%, 50%, and 100% ambient natural sunlight) and one of five dilutions of water accommodated fractions of two naturally weathered source oils. A dose dependent effect of PAH and UV on larval mortality was observed. Mortality was markedly higher in PAH treatments that included co-exposure to more intense UV light. PAH treatments under low intensity sunlight had relatively high survival. These data demonstrate the importance of considering combined effects of non-chemical (i.e. UV exposure) and chemical stressors and the potential for photo-induced effects after exposure to PAH following the Deepwater Horizon spill.
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Plankton Community Response to Dechorination of a Municipal Effluent Discharged into the Trinity River

Plankton Community Response to Dechorination of a Municipal Effluent Discharged into the Trinity River

Date: December 1994
Creator: Bryan, Brynne L. (Brynne Lee)
Description: Chorine is used by the Village Creek Waste Water Treatment Plant to kill pathogenic microorganisms prior to discharge of the effluent into the Trinity River. The residual chlorine in the river impacted aquatic life prompting the U.S. Environmental Protection Agency in December 1990 to require dechlorination using sulfur dioxide. One pre-dechlorination and four post-dechlorination assessments of phytoplankton, periphyton, and zooplankton communities were conducted by the Institute of Applied Sciences at the University of North Texas. Dechlorination had no effect on the phytoplankton community. The periphyton community exhibited a shift in species abundance with a more even distribution of organisms among taxa. No change occurred in zooplankton species abundance, however, there was a decrease in zooplankton density following dechlorination.
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Population Dynamics of Zebra Mussels (Dreissena Polymorpha) in a North Texas Reservoir: Implications for Invasions in the Southern United States

Population Dynamics of Zebra Mussels (Dreissena Polymorpha) in a North Texas Reservoir: Implications for Invasions in the Southern United States

Date: December 2013
Creator: Churchill, Christopher J.
Description: This dissertation has two main objectives: first, quantify the effects of environmental conditions on spatio-temporal spawning and larval dynamics of zebra mussels (Dreissena polymorpha [Pallas 1771]) in Lake Texoma, and second, quantify the effects of environmental conditions on survival, growth, and reproduction of young of the year (YOY) juvenile zebra mussels. These biological responses directly influence population establishment success and invasive spread dynamics. Reproductive output of the zebra mussel population in Lake Texoma was significantly related to water temperature and lake elevation. Annual maximum larval (veliger) density decreased significantly indicating a population crash, which was likely caused by thermal stress and variability of lake elevation. In 2011, temperatures peaked at 34.3°C and lake elevation decreased to the lowest level recorded during the previous 18 years, which desiccated a substantial number of settled mussels in littoral zones. Estimated mean date of first spawn in Lake Texoma was observed approximately 1.5 months earlier than in Lake Erie, and peak veliger densities were observed two months earlier. Veligers were observed in the deepest oxygenated water after lake stratification. During a 69-day in situ experiment during summer in Lake Texoma, age-specific mortality of zebra mussels was generally high until temperatures decreased to approximately 28°C, ...
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Portrait of Your Stream: Development and Assessment of a Stream Ecology Program for Middle-School Student

Portrait of Your Stream: Development and Assessment of a Stream Ecology Program for Middle-School Student

Date: May 2001
Creator: Swirczynski, Brenda J.
Description: Portrait of Your Stream (POYS) is a stream ecology and student action program designed for use with middle-school students. The program is correlated with learning cycle pedagogical methods emphasizing student-centered lessons and activities in both classroom and outdoor settings. Implementation of a pilot program in the Fall semester of 1999 was used to collect formal and informal responses and data from students and teachers. Data included changes in student knowledge, skills and attitudes and were analyzed for determination of the success of program objectives and modifications to the program. The final POYS program is currently distributed and administered by the Botanical Research Institute of Texas.
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The Potential of Coelomocyte Chemotaxis as an Immune Biomarker in the Earthworm, Lumbricus terrestris

The Potential of Coelomocyte Chemotaxis as an Immune Biomarker in the Earthworm, Lumbricus terrestris

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Date: December 1999
Creator: Mota, Jennifer A.
Description: Coelomocyte migration responses, both random and chemotatic, were examined in the earthworm Lumbricus terrestris. Coelomocyte random migration patterns towards non-stimulatory, non-chemotatic solutions were described. Migration responses to immunostimulatory agents lipopolysaccharides (LPS), N-formly-methionyl-leucyl-phenylalanine (FMLP), sheep erythrocytes, Saccharomyces cerevisiae, Aeromonas hydrophila, Eisenia fetida and Rhabditis pellio were characterized. Chemotaxis was reported to LPS, FMLP, sheep erythrocytes, S. cerivesae and E. fetida. Bio-indicator potential of chemotaxis is discussed relative to variability in migration responses.
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Pretreatment Optimization of Fiberglass Manufacturing Industrial Wastewater

Pretreatment Optimization of Fiberglass Manufacturing Industrial Wastewater

Date: December 1998
Creator: Dragoo, Ron
Description: Wastewater effluent produced in the fiberglass manufacturing industry contains a significant amount of total suspended solids. Environmental regulations require pretreatment of effluent before it is discharged to the municipal wastewater treatment plant. Chemical precipitation by coagulation and flocculation is the method of pretreatment used at the Vetrotex CertainTeed Corporation (VCT). A treatability study was conducted to determine conditions at which the VCT Wastewater Pretreatment Plant could operate to consistently achieve a total suspended solids concentration ≤ 200-mg/L. Jar tests varied pH, polymer dosage, and ferric sulfate dosage. Total suspended solids and turbidity were measured to evaluate treatment performance. The data were used to determine an optimum set of conditions under project guidelines. Of twelve polymers screened, BPL 594 was selected as the most effective polymer. For cost efficiency in the wastewater pretreatment operation, recommendations suggested that treatment chemical injection be electronically controlled according to turbidity of the treated effluent.
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Quantifying Forest Vertical Structure to Determine Bird Habitat Quality in the Greenbelt Corridor, Denton, Tx

Quantifying Forest Vertical Structure to Determine Bird Habitat Quality in the Greenbelt Corridor, Denton, Tx

Date: August 2013
Creator: Matsubayashi, Shiho
Description: This study presents the integration of light detection and range (LiDAR) and hyperspectral remote sensing to create a three-dimensional bird habitat map in the Greenbelt Corridor of the Elm Fork of the Trinity River. This map permits to examine the relationship between forest stand structure, landscape heterogeneity, and bird community composition. A biannual bird census was conducted at this site during the breeding seasons of 2009 and 2010. Census data combined with the three-dimensional map suggest that local breeding bird abundance, community structure, and spatial distribution patterns are highly influenced by vertical heterogeneity of vegetation surface. For local breeding birds, vertical heterogeneity of canopy surface within stands, connectivity to adjacent forest patches, largest forest patch index, and habitat (vegetation) types proved to be the most influential factors to determine bird community assemblages. Results also highlight the critical role of secondary forests to increase functional connectivity of forest patches. Overall, three-dimensional habitat descriptions derived from integrated LiDAR and hyperspectral data serve as a powerful bird conservation tool that shows how the distribution of bird species relates to forest composition and structure at various scales.
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Rainfall-runoff changes due to urbanization: a comparison of different spatial resolutions for lumped surface water hydrology models using HEC-HMS.

Rainfall-runoff changes due to urbanization: a comparison of different spatial resolutions for lumped surface water hydrology models using HEC-HMS.

Date: December 2005
Creator: Redfearn, Howard Daniel
Description: Hydrologic models were used to examine the effects of land cover change on the flow regime of a watershed located in North-Central Texas. Additionally, the effect of spatial resolution was examined by conducting the simulations using sub-watersheds of different sizes to account for the watershed. Using the Army Corps of Engineers, Hydrologic Engineering Center Hydrologic Modeling System (HEC-HMS), two different modeling methods were evaluated at the different sub-watershed resolutions for four rainfall events. Calibration results indicate using the smaller spatial resolutions improves the model results. Different scenarios for land cover change were evaluated for all resolutions using both models. As land cover change increased, the amount of flow from the watershed increased.
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Recovery of the Fish Population of a Municipal Wastewater Dominated, North Texas Creek After a Major Chlorine Disturbance

Recovery of the Fish Population of a Municipal Wastewater Dominated, North Texas Creek After a Major Chlorine Disturbance

Date: August 2002
Creator: Maschmann, Gerald F.
Description: This study evaluated the effects of a major chlorine disturbance on fish communities in Pecan creek by the City of Denton's Pecan Creek Water Reclamation Plant. Fish communities in Pecan Creek were sampled using a depletion methodology during February, April, July, and November, 1999. February and April sampling events showed that the fish communities were severely impacted by the chlorine. Sampling during July and November showed fish communities recovered in Pecan Creek. The first-twenty minutes of shocking and seining data were analyzed to mirror an equal effort methodology. This methodology was compared to the depletion methodology to see if the equal effort methodology could adequately monitor the recovery of Pecan Creek after the chlorine disturbance. It was determined that the equal effort methodology was capable of monitoring the recovery of Pecan Creek, but could not accurately represent the fisheries community as well as the depletion method. These data using the twenty-minute study were compared to a previous study. Results of this study were similar to those found in a previous study, although fish communities were more severely impacted and took longer to recover.
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Removal of selected water disinfection byproducts, and MTBE in batch and continuous flow systems using alternative sorbents.

Removal of selected water disinfection byproducts, and MTBE in batch and continuous flow systems using alternative sorbents.

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Date: December 2002
Creator: Kadry, Ahmed Y.
Description: A study was conducted to evaluate the sorption characteristics of six disinfection byproducts (DBPs) on four sorbents. To investigate sorption of volatile organic compounds (VOCs), specially designed experimental batch and continuous flow modules were developed. The investigated compounds included: chloroform, 1,2-dichloroethane (DCE), trichloroethylene (TCE), bromodichloromethane (BDCM), methyl tertiary butyl ether (MTBE), bromate and bromide ions. Sorbents used included light weight aggregate (LWA), an inorganic porous material with unique surface characteristics, Amberlite® XAD-16, a weakly basic anion exchange resin, Amberjet®, a strongly basic anion exchange resin, and granular activated carbon (GAC). Batch experiments were conducted on spiked Milli-Q® and lake water matrices. Results indicate considerable sorption of TCE (68.9%), slight sorption of bromate ions (19%) and no appreciable sorption for the other test compounds on LWA. The sorption of TCE increased to 75.3% in experiments utilizing smaller LWA particle size. LWA could be a viable medium for removal of TCE from contaminated surface or groundwater sites. Amberlite® was found unsuitable for use due to its physical characteristics, and its inability to efficiently remove any of the test compounds. Amberjet® showed an excellent ability to remove the inorganic anions (>99%), and BDCM (96.9%) from aqueous solutions but with considerable elevation of pH. ...
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Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone

Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone

Date: May 2011
Creator: Paulos, Peter M.
Description: A commonly prescribed contraceptive, the synthetic progestin norethindrone (NET) inhibits ovulation in humans. However, ecotoxicological data are lacking. Preliminary tests produced an LC50 for NET of > 1.0 mg/L (96-hour, fathead minnow (FHM) and medaka) and a NOEC of 242.0 µg/L, a LOEC of 485.0 µg/L (7-day, growth for FHM and medaka). Reproductive testing revealed a LOEC for fecundity of 24.1 ng/L (21 days, medaka). Further testing confirmed the LOEC of 24.1 ng/L while defining a NOEC of 4.7 ng/L (28 days, medaka). Effect of NET in medaka life-cycle exposure at concentrations exceeding 4.7 ng/L was evident. Few females were present in the 24.7 ng/L exposure concentration, with none in the 104.6 ng/L. Egg production was significantly reduced at concentrations exceeding 4.7 ng/L. Additionally, weight, condition factor and somatic indices were significantly different in males exposed to concentrations exceeding 4.7 ng/L. For fecundity and sexual differentiation; the NOEC was 4.7 ng/L, the LOEC 24.6 ng/L; growth and somatic indices, the NOEC was more appropriately 0.9 ng/L, with effect evident at 4.7 ng/L. Sexual differentiation of the F1 population was similar to the F0. A defining result of this test was development of exceptionally large ovaries in NET- exposed female medaka, ...
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Residential Grid-Connected Photovoltaics Adoption in North Central Texas: Lessons from the Solarize Plano Project

Residential Grid-Connected Photovoltaics Adoption in North Central Texas: Lessons from the Solarize Plano Project

Date: August 2016
Creator: Jack, Katherine G.
Description: Residential Grid-Connected Photovoltaics (GPV) systems hold remarkable promise in their potential to reduce energy use, air pollution, greenhouse gas emissions, and energy costs to consumers, while also providing grid efficiency and demand-side management benefits to utilities. Broader adoption of customer-sited GPV also has the potential to transform the traditional model of electricity generation and delivery. Interest and activity has grown in recent years to promote GPV in north central Texas. This study employs a mixed methods design to better understand the status of residential GPV adoption in the DFW area, and those factors influencing a homeowner's decision of whether or not to install a system. Basic metrics are summarized, including installation numbers, distribution and socio-demographic information for the case study city of Plano, the DFW region, Texas, and the United States. Qualitative interview methods are used to gain an in-depth understanding of the factors influencing adoption for the Solarize Plano case study participants; to evaluate the effectiveness of the Solarize Plano program; and to identify concepts that may be regionally relevant. Recommendations are presented for additional research that may advance GPV adoption in north central Texas.
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Revisiting Aldo Leopold's "Perfect" Land Health: Conservation and Development in Mexico's Rio Gavilan

Revisiting Aldo Leopold's "Perfect" Land Health: Conservation and Development in Mexico's Rio Gavilan

Date: December 2004
Creator: Forbes, William
Description: The Rio Gavilan watershed, located in Mexico 's northern Sierra Madre Occidental , has significance in conservation history. Upon visiting the remote, largely un­developed watershed during two hunting trips in the 1930s, renowned conservationist Aldo Leopold thought it was the best picture of land health he had seen. His main indicators of healthy land were slow water runoff rates regulating erosion and historical predator-prey relationships. The visits confirmed Leopold's concept of land health, inspired many of his essays, and helped shape his land ethic. Leopold proposed the area as a control site to research healthy land throughout North America . The proposal never went forward and the area has since been more intensively logged and grazed. This dissertation research used extensive literature review, archives, oral histories, citizen surveys, and rapid assessment of forest, rangeland, riparian, and socioeconomic health to assess impacts of past cultures and update the area's land health status. Projects that could restore land health, such as linked eco-tourism, forest density reduction, and rotational grazing, were assessed for feasibility. Recent critiques of Leopold's land ethic were also reviewed. Results indicate most pre-1940s impacts were light, current land health status is moderate, and local interest exists in restoring land ...
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Riparian Forest Width and the Avian Community in a Greenbelt Corridor Setting

Riparian Forest Width and the Avian Community in a Greenbelt Corridor Setting

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Date: May 2001
Creator: Hoffman, Karl W.
Description: The forest avian community of the Ray Roberts Greenbelt (Denton Co., Texas) was characterized for two years using point count station sampling, from fall 1998 to summer 2000. Richness data for both breeding seasons were correlated with two-spatial metrics: width of the riparian forest and distance to the nearest edge. There were significant correlations between forest interior species richness and both spatial metrics, for both breeding seasons. Based on these data, a minimum riparian forest width threshold of 400-meters is suggested to provide habitat for forest interior species, which have lost considerable habitat through forest fragmentation. Partners in Flight breeding bird priority concern scores were used to create a habitat priority index for the Trinity River bottomland hardwood forest system
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Role of N-Acylethanolamines in Plant Defense Responses: Modulation by Pathogens and Commercial Antimicrobial Stressors

Role of N-Acylethanolamines in Plant Defense Responses: Modulation by Pathogens and Commercial Antimicrobial Stressors

Date: August 2010
Creator: Vadapalli, Vatsala
Description: N-acyl ethanolamines (NAEs) are a class of lipids recently recognized as signaling molecules which are controlled, in part, by their degradation by fatty acid amide hydrolase (FAAH). On the basis of previous studies indicating increased NAE levels in a tobacco cell suspension-xylanase elicitor exposure system and the availability of FAAH mutants, overexpressor and knockout (OE and KO) genotypes in Arabidopsis thaliana, further roles of NAEs in A. thaliana plant defense was investigated. The commonly occurring urban antimicrobial contaminant triclosan (TCS) has been shown to suppress lipid signaling associated with plant defense responses. Thus, a second objective of this study was to determine if TCS exposure specifically interferes with NAE levels. No changes in steady state NAE profiles in A. thaliana-Pseudomonas syringae pv. syringae and A. thaliana-flagellin (bacterial peptide, flg22) challenge systems were seen despite evidence that defense responses were activated in these systems. There was a significant drop in enoyl-ACP reductase (ENR) enzyme activity, which catalyzes the last step in the fatty acid biosynthesis pathway in plants, on exposure of the seedlings to TCS at 10 ppm for 24 h and decreased reactive oxygen species (ROS) production due to flg22 in long term exposure of 0.1 ppm and short term ...
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The Role of Rainfed Farm Ponds in Sustaining Agriculture and Soil Conservation in the Dry High Valley Region of Cochabamba, Bolivia: Design Considerations and Post Impoundment Analysis

The Role of Rainfed Farm Ponds in Sustaining Agriculture and Soil Conservation in the Dry High Valley Region of Cochabamba, Bolivia: Design Considerations and Post Impoundment Analysis

Date: August 1999
Creator: Kuiper, John R.
Description: Lack of sufficient water for irrigation is a major problem in and around the valleys surrounding the town of Aiquile, Cochabamba Bolivia. In addition, much of the region is undergoing desertification compounded by drought, deforestation, bad traditional agricultural practices, over grazing and a "torrential" rainfall pattern leading to severe soil erosion and low agricultural production. Between 1992 and 1994, the author constructed a network of 24 small, mostly rainfed farm ponds to increase agricultural production and alleviate soil erosion and land-use problems by improving cover conditions. A 5-year post-impoundment analysis was carried out in 1998. The analysis examined current pond conditions, design criteria, irrigation water / crop production increases and the alleviation of land-use problems. Current pond conditions fell into four distinct categories with only 25 percent of the ponds being deemed as "functioning well." The project increased irrigation in the region and improved cover conditions in 66 percent of the pond sites.
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Selection and Use of Aquatic Vegetation by Migratory Waterfowl in North Central Texas

Selection and Use of Aquatic Vegetation by Migratory Waterfowl in North Central Texas

Date: May 2001
Creator: Smith, JoEtta Kaye
Description: Assessment of aquatic plant selection by waterfowl has been conducted during the winters of 1997-2000 on 49 0.2-0.79 ha research ponds in north central Texas. Ponds were categorized by dominant plant species into eight habitat types. Census with waterfowl species identification were performed to investigate impacts of aquatic vegetation and water depth on waterfowl. Eighteen waterfowl species were observed. Peak migration occurred in late December/early January. Mixed native ponds and mixed native/hydrilla ponds were the most frequently selected habitat types. The study included correlation analysis between pond water levels and waterfowl use. Full ponds received greatest use followed by half full ponds, while almost empty ponds received minimal use. Time activity budgets were conducted on waterfowl utilizing mixed native and hydrilla ponds to compare waterfowl time partitioning on native aquatic vegetation versus hydrilla. Although only minor differences were found in time budgets, social status appears to be strongly related to habitat selection. Ducks on native ponds were paired (86%), conversely no ducks on hydrilla ponds were paired. Hydrilla pond although frequently utilized, were populated by lower status birds mostly single hens.
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Simulating Thermal and Chemical Spills in Coupled Cooling Reservoirs

Simulating Thermal and Chemical Spills in Coupled Cooling Reservoirs

Date: August 1997
Creator: Qiu, Bin
Description: Hot water discharges and potential chemical spills are factors that threaten water quality in cooling reservoirs of chemical and power plants. In this thesis, three models are used to analyze the impact of these factors in a particular case study.
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Simulation of local watershed nutrient and sediment delivery to Lake Texoma.

Simulation of local watershed nutrient and sediment delivery to Lake Texoma.

Date: May 2004
Creator: Upton, Alexandra C.
Description: A hydrologic model and watershed export model was used to estimate the loading of nutrients and total suspended solids from un-gaged local watersheds associated with Lake Texoma. Discharge to the reservoir from local watersheds was predicted using a modification of the curve number method in HEC Hydrologic Modeling System (HEC-HMS). Event mean concentrations were applied to land use to estimate loadings of nutrients and TSS. Total discharge from the local watersheds was estimated to be 3.02 x 107 cubic meters for a study window of March 1 to May 31, 1997, less than 10 percent of the input to the lake from the Red River and Washita River systems. Loadings were estimated to be 33,553 kg nitrogen, 4,401 kg phosphorus, and 3,423,140 kg TSS. The models and results obtained from their application appear to have potential utility for use in a water quality management decision support system for the reservoir.
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Simulation of physical and chemical processes in reservoirs: Two case studies.

Simulation of physical and chemical processes in reservoirs: Two case studies.

Date: December 2005
Creator: García Iturbe, Selma L.
Description: Managing water quality aspects requires the use of integrative tools that allow a holistic approach to this problem. Water quality models coupled to hydrodynamic models are these tools. This study presents the application of the water quality model WASP coupled to the hydrodynamic model DYNHYD for two distinct reservoirs: Lake Texoma and Tocoma Reservoir. Modeling the former included simulations of water velocities, water level, and four chemical and physical compounds: chlorides, dissolved oxygen (DO), biochemical oxygen demand (BOD), and total suspended solids (TSS); and validation of the results by comparing with observed values during March - May, 1997. The latter is still under project status and the simulation was performed in a prospective way. The analysis included simulations of water velocities under current and for expected conditions, DO and BOD. Both models, DYNHYD and WASP, fitted pretty well to observed conditions for Lake Texoma and for where Tocoma Reservoir has been planned. Considering management and decision support purposes, the role of boundary and loading conditions also was tested. For Lake Texoma, controlling boundary conditions for chlorides is a determinant factor for water quality of the system. However, DO and TSS in the reservoir are governed by additional process besides the ...
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Soil and Forest Variation by Topography and Succession Stages in the Greenbelt Corridor, Floodplain of the Elm Fork of the Trinity River, North Texas.

Soil and Forest Variation by Topography and Succession Stages in the Greenbelt Corridor, Floodplain of the Elm Fork of the Trinity River, North Texas.

Date: August 2011
Creator: Rijal, Rajan
Description: The Greenbelt Corridor (GBC), located in a floodplain of the Elm Fork of the Trinity River, contains patches of bottomland forest and serves as part of Lake Lewisville’s flood control backwaters. This study examines forest structure and composition in relation to topographic position and forest stage in the GBC. Thirty two plots were surveyed within various stage classes, topographic positions, and USDA soil types. Trees were identified and measured for height and DBH. Density, basal area, and importance value for each of species was calculated. Soil and vegetation were analyzed using ANOVA, Principal Component Analysis, Canonical Correlation, Canonical Correspondence Analysis and Cluster Analysis. Tests confirmed that calcium carbonate and pH show significant differences with topographic positions but not with forest stage. Potassium shows no significant difference with soil texture class. Sand shows a strong negative correlation with moisture, organic matter, organic carbon and negative correlation with calcium carbonate and potassium. Silt shows positive correlation with moisture, organic matter, organic carbon, and calcium carbonate. Clay shows strong positive correlation with moisture, organic matter and organic carbon but negative correlations with pH. Swamp privet is dominant tree types in wetland forest. Sugarberry cedar elm, green ash and American elm are widely distributed ...
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Solid phase microextraction of amino-dinitrotoluenes in tissue.

Solid phase microextraction of amino-dinitrotoluenes in tissue.

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Date: December 2004
Creator: Tsui-Bowen, Alethea
Description: TNT (2,4,6-trinitrotoluene) readily and predominantly transforms to 2ADNT (2-amino-4,6-dinitrotoluene) and 4ADNT (4-amino-2,6-dinitrotoluene) in environmental matrixes and tissues. Solid phase microextraction (SPME) was used to extract ADNTs (amino-dinitrotoluenes) from tissue as a potential method to investigate the recalcitrance of metabolically-generated ADNTs versus absorbed ADNTs. Tubifex tubifex was allowed to metabolize TNT into ADNTs in 24-hr static non-renewal exposure test followed by 24-hr depuration in clean reconstituted hard water. Polyacrylate-coated (PA) SPME fibers were then deployed and agitated in tissue homogenates containing metabolically-generated ADNTs for 48 hr to provide a measure of available ADNTs. Extractability of ADNTs from T. tubifex tissue containing metabolically-generated ADNTs was significantly less than extractability of ADNTs from T. tubifex tissue containing absorbed ADNTs: 50-60% and 81-90% of expected extractability based on fiber-water partition ratio. The lower SPME extractability of metabolically-generated ADNTs may stem from the unavailability of metabolically-generated ADNTs sequestered in tissue or bound to tissue macromolecules during metabolism of TNT to ADNT. Tissue extractions using SPMEs may be able to estimate such bound organic residues in tissue and serve as potential indicators of toxicological bioavailability and biomagnification potential of tissue-associated organic compounds.
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Spatial Analysis of Atrazine in the Elm Fork Watershed

Spatial Analysis of Atrazine in the Elm Fork Watershed

Date: May 2000
Creator: Ochandio, Mario Roberto
Description: This study assessed the water quality of the Elm Fork Watershed with regards to the herbicide Atrazine. Atrazine is a potential environmental endocrine disruptor and carcinogen. Overall, concentrations were lower than the four-quarter drinking water average of 3 µg/Lthe Maximum Contaminant Level set by the USEPA. However, three creek stations had four-quarter average concentrations greater than 3 µg/L, and virtually all samples exceeded the 0.1 µg/L standard set in Europe [1,2]. Statistically significant differences in concentrations were detected between the 27 sampling stations and areas of high concentrations were identified. However correlations between Atrazine concentrations and land-use and precipitation were not statistically significant. Further analysis with more detailed data should be conducted before any relationships are discarded.
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Spatial and Temporal Patterns of Areal and Volumetric Phytoplankton Productivity of Lake Texoma

Spatial and Temporal Patterns of Areal and Volumetric Phytoplankton Productivity of Lake Texoma

Date: August 2001
Creator: Baugher, Tessy
Description: Phytoplankton productivity of Lake Texoma was measured for one year from August 1999 to August 2000 for four stations, using the oxygen change method and laboratory incubation. Mean values of the photosynthetic parameters, PBmax and alphaB ranged from 4.86 to 46.39 mg O2.mg Chl-1.hr-1 for PBmax and 20.06 to 98.96 mg O2.mg Chl-1.E-1.m2 for alphaB. These values were in the range to be expected for a highly turbid, temperate reservoir. Estimated gross annual areal productivity ranged from 594 g C.m2.yr-1 (P.Q. = 1.2), at a station in the Washita River Zone to 753 g C.m2.yr-1 at a station in the Red River Zone, of the reservoir. Gross annual areal productivity at Station 17, in the Main Lake Zone, was 708 g C.m2.yr-1. Gross areal and volumetric productivity showed distinct seasonal variation with Photosynthetically Available Radiation (PAR) and temperature. Trophic status estimated on a station-by-station basis, using net productivity values derived from gross productivity and respiration estimates, was mesotrophic for all the stations, though one station approached eutrophy. Net productivity values ranged from 0.74 to 0.91 g C. m-2.d-1. An algal bioassay conducted at two stations in August 2000, revealed that phosphorus was most likely the nutrient limiting photosynthesis at both ...
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