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 Collection: UNT Theses and Dissertations
N-Acylethanolamine metabolism during seed germination: Molecular identification of a functional N-acylethanolamine amidohydrolase.

N-Acylethanolamine metabolism during seed germination: Molecular identification of a functional N-acylethanolamine amidohydrolase.

Date: August 2004
Creator: Shrestha, Rhidaya
Description: N-Acylethanolamines (NAEs) are endogenous lipid metabolites that occur in a variety of dry seeds, and their levels decline rapidly during the first few hours of imbibition (Chapman et al., 1999, Plant Physiol., 120:1157-1164). Biochemical studies supported the existence of an NAE amidohydrolase activity in seeds and seedlings, and efforts were directed toward identification of DNA sequences encoding this enzyme. Mammalian tissues metabolize NAEs via an amidase enzyme designated fatty acid amide hydrolase (FAAH). Based on the characteristic amidase signature sequence in mammalian FAAH, a candidate Arabidopsis cDNA was identified and isolated by reverse transcriptase-PCR. The Arabidopsis cDNA was expressed in E. coli and the recombinant protein indeed hydrolyzed a range of NAEs to free fatty acids and ethanolamine. Kinetic parameters for the recombinant protein were consistent with those properties of the rat FAAH, supporting identification of this Arabidopsis cDNA as a FAAH homologue. Two T-DNA insertional mutant lines with disruptions in the Arabidopsis NAE amidohydrolase gene (At5g64440) were identified. The homozygous mutant seedlings were more sensitive than the wild type to exogenously applied NAE 12:0. Transgenic seedlings overexpressing the NAE amidohydrolase enzyme showed noticeably greater tolerance to NAE 12:0 than wild type seedlings. These results together provide evidence in vitro ...
Contributing Partner: UNT Libraries
N-Acylethanolamine (NAE) profiles change during Arabidopsis thaliana seed germination and seedling growth.

N-Acylethanolamine (NAE) profiles change during Arabidopsis thaliana seed germination and seedling growth.

Date: August 2006
Creator: Wiant, William C.
Description: An understanding of the potential roles as lipid mediators of a family of bioactive metabolites called N-acylethanolamines (NAEs) depends on their accurate identification and quantification. The levels of 18C unsaturated NAEs (e.g. NAE18:2, NAE 18:3, etc.) in wild-type seeds (about 2000 ng/g fw) generally decreased by about 80% during germination and post-germinative growth. In addition, results suggest NAE-degradative fatty acid amide hydrolase (FAAH) expression does not play a major role in normal NAE metabolism as previously thought. Seedlings germinated and grown in the presence of abscisic acid (ABA), an endogenous plant hormone, exhibited growth arrest and secondary dormancy, similar to the treatment of seedlings with exogenous N­lauroylethanolamine (NAE12:0). ABA-mediated growth arrest was associated with higher levels of unsaturated NAEs. Overall, these results are consistent with the concept that NAE metabolism is activated during seed germination and suggest that the reduction in unsaturated NAE levels is under strict temporal control and may be a requirement for normal seed germination and post-germinative growth.
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ADA Compliance and Accessibility of Aquatic Facilities in the North Texas Area

ADA Compliance and Accessibility of Aquatic Facilities in the North Texas Area

Date: May 2007
Creator: Pike, Hilary Eryn
Description: The purpose of this study was to determine the degree to which existing aquatic facilities in the North Texas metroplex complied with the 1991 Americans with Disabilities Act Accessibility Guidelines (ADAAG) and the proposed Americans with Disabilities Act Accessibility Guidelines: Recreation Facilities (ADAAG supplement). Fifty-two aquatic facilities were evaluated based on: parking lot, ticket counter, gate/entry, restroom, dressing area, drinking fountain, pathway, and pool entry method structural domains. Physical measurements and a few direct observations were recorded on the survey instrument. Surveys were then reviewed and facility scores were tabulated. No facility was found to be 100% compliant with ADAAG and the ADAAG supplement. Aquatic facilities are already struggling to catch up with the 1991 ADAAG, but when the United States Department of Justice approves the proposed ADAAG supplement, aquatic facilities will fall even further behind.
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The Adagio of Mahler's Ninth Symphony: A Schenkerian Analysis and Examination of the Farewell Story

The Adagio of Mahler's Ninth Symphony: A Schenkerian Analysis and Examination of the Farewell Story

Date: May 2011
Creator: Patterson, Jason
Description: Mahler's Ninth Symphony, since its premier in 1912, has sparked much debate about its programmatic meaning. This thesis provides an in-depth analysis of the Adagio and an examination of the controversy of the farewell story. In the process of the analysis I have compared my findings to some of the important authors in Mahler's field such as Vera Micznik, Henry-Louis de La Grange, and Christopher Orlo Lewis. Some of the conclusions are that a closer investigation of the music is necessary and that the programmatic reading of the farewell story can be appropriate.
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Adam Smith Revisited

Adam Smith Revisited

Date: January 1967
Creator: Roden, Peyton Foster
Description: This study represents an interpretation of Adam Smith's attitude toward a commercial society based upon natural liberty. In developing the thesis that the two works, The Theory of Moral Sentiments and Wealth of Nations, are logically consistent, it is necessary to establish the reason of reasons why Adam Smith embraced a commercial society based upon the "obvious and simple system of natural liberty." It will be established that he embraced such as system because it allowed the maximum accumulation of capital, which is the manifestation of being truly virtuous as developed in his Moral Sentiments. To go one step further, it will be seen that, so far as Smith is concerned, it is the rising bourgeoisie, the manufacturers and artificers, which is truly virtuous, i.e., accumulates capital.
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The Adaptability and Present Status of Andropogon Ischaemum L. in Denton County, Texas

The Adaptability and Present Status of Andropogon Ischaemum L. in Denton County, Texas

Date: 1951
Creator: Belders, Floyd William
Description: This investigation has been made with reference to the adaptability of Andropogon ischaemum, King Ranch bluestem, to various soils, various slopes, and various erosion conditions in Denton County.
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Adapting a Reading Program to Individual Differences

Adapting a Reading Program to Individual Differences

Date: August 1954
Creator: Manning, Lucile
Description: The problem of this study was to test the effectiveness of adapting the reading instruction to the level and ability of individuals or small groups of a particular group of second grade children.
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Adapting Filial Therapy for Families who have a Child with a Life-Threatening Illness

Adapting Filial Therapy for Families who have a Child with a Life-Threatening Illness

Date: August 2004
Creator: Steen, Rheta LeAnne
Description: Utilizing a collective case study design, I examined and described the filial therapy (FT) process and adaptations discovered to be necessary and unnecessary in working with families who have a child with a life-threatening illness in the hospital setting. Data from a total of 7 parents was utilized, including those who terminated early, in order to gain a greater understanding of adapting FT for families who have a child with a life-threatening illness and their participation patterns. The parents attended 10 one- to two-hour FT sessions. The data was analyzed to examine for themes, patterns and relationships intrinsically with each case participant, as well as across cases. Analysis indicated that parents with a child with a life-threatening illness had great difficulty committing to attend FT; and a high rate of attrition occurred for those who did commit. A theme regarding flexibility was found to be of eminent importance in a variety of manifestations including therapeutic methods, session format, location and time of sessions, and intense vs traditional FT. Therapeutic adaptations in flexibility found to be important including openness to cathartic and personal parenting sessions, tolerance of forgetfulness, and lowering typical therapeutic concerns of dependency in the relationship. An inability for ...
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Adapting on the Plains: the United States Army's Evolution of Mobile Warfare in Texas, 1848-1859

Adapting on the Plains: the United States Army's Evolution of Mobile Warfare in Texas, 1848-1859

Date: May 2013
Creator: Buchy, Mark B.
Description: The Army, despite having been vexed for a century on how to effectively fight the Plains Indians, ultimately defeated them only a decade after the Civil War. This thesis will bring to the forefront those individuals who adapted fighting techniques and ultimately achieved victories on the Texas frontier before the Civil War. The majority of these victories came as a result of mounted warfare under the direction of lower ranking officers in control of smaller forces. The tactic of fighting Indians from horseback was shown to be effective by the Rangers and later emulated by the Army.
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Adaptive Advantages of Carotenoid Pigments in Alpine and Subalpine Copepod Responses to Polycyclic Aromatic Hydrocarbon Induced Phototoxicity

Adaptive Advantages of Carotenoid Pigments in Alpine and Subalpine Copepod Responses to Polycyclic Aromatic Hydrocarbon Induced Phototoxicity

Date: May 2010
Creator: Kovach, Matthew James
Description: Alpine zooplankton are exposed to a variety of stressors in their natural environment including ultraviolet radiation. Physiological coping mechanisms such as the accumulation of photoprotective compounds provide these zooplankton protection from many of these stressors. Elevated levels of carotenoid compounds such as astaxanthin have been shown to help zooplankton survive longer when exposed to ultraviolet radiation presumably due to the strong antioxidant properties of carotenoid compounds. This antioxidant capacity is important because it may ameliorate natural and anthropogenic stressor-induced oxidative stress. While previous researchers have shown carotenoid compounds impart increased resistance to ultraviolet radiation in populations of zooplankton, little work has focused on the toxicological implications of PAH induced phototoxicity on zooplankton containing high levels of carotenoid compounds. This thesis discusses research studying the role that carotenoid compounds play in reducing PAH induced phototoxicity. By sampling different lakes at elevations ranging from 9,500' to 12,700' in the front range of the Colorado Rocky Mountains, copepod populations containing different levels of carotenoid compounds were obtained. These populations were then challenged with fluoranthene and ultraviolet radiation. Results discussed include differences in survival and levels of lipid peroxidation among populations exhibiting different levels of carotenoid compounds, and the toxicological and ecological implications of ...
Contributing Partner: UNT Libraries