Identification and Characterization of the Pyrimidine Biosynthetic Operon in Streptomyces griseus
Description:
To further understand the ATCase/DHOase bifunctional complex formed in Streptomyces, the genes encoding these and other pyrimidine enzymes were identified and characterized. Polymerase chain reaction (PCR) was utilized in this effort. Primers were constructed by selecting conserved regions of
pyrimidine genes from known gene and protein sequences of a wide variety of organisms. These sequences were then optimized to Streptomyces codon usage. PCR products were obtained from internal sites within…
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Date:
May 1998
Creator:
Hooten, Jody J. (Jody Jeran)
Partner:
UNT Libraries