Description: The primary purpose of this investigation is not only to improve the present technique of culture of Mycobacterium tuberculosis, but also to make a comparative study of the media use, namely, Veterans Administration modification of Trudeau's medium, Lowenstein's egg medium, and that developed by the author, in order to find which, if any, of these will most easily and effectively meed the needs of the hospital laboratory.

Description: This thesis attempted to study the bottom productivity both qualitatively and quantitatively with reference to the distribution of bonthos. The study of the bottom fauna in large reservior lakes is a relatively new field. This work will give more information on an unknown field than previously existed, although its scope is not intend to be exhaustive.

Description: The ability to synthesize pyrimidine nucleotides is essential for most organisms. Pyrimidines are required for RNA and DNA synthesis, as well as cell wall synthesis and the metabolism of certain carbohydrates. Recent findings, however, indicate that the pyrimidine biosynthetic pathway and its intermediates maybe more important for bacterial metabolism than originally thought. Maksimova et al., 1994, reported that a P. putida M, pyrimidine auxotroph in the third step of the pathway, dihydroorotase (DHOase), failed to produce the siderophore pyoverdin. We created a PAO1 DHOase pyrimidine auxotroph to determine if this was also true for P. aeruginosa. Creation of this mutant was a two-step process, as P. aeruginosa has two pyrC genes (pyrC and pyrC2), both of which encode active DHOase enzymes. The pyrC gene was inactivated by gene replacement with a truncated form of the gene. Next, the pyrC2 gene was insertionally inactivated with the aacC1 gentamicin resistance gene, isolated from pCGMW. The resulting pyrimidine auxotroph produced significantly less pyoverdin than did the wild type. In addition, the mutant produced 40% less of the phenazine antibiotic, pyocyanin, than did the wild type. As both of these compounds have been reported to be vital to the virulence response of P. aeruginosa, ...

Description: The purpose of this study was to correlate the Vegetation of the Woodbine Sands with the edaphic factors. In the laboratory an analysis of the edephic factors was made of the twenty-two soil types collected from the three formations. The results of these and other analysis are shown in tables and graphs. The results indicate that the vegetational cover of an area that is uniform in its origin and in its resident soil factors is determined by the edaphic factors present.

Description: The problem has consisted, first, of a mapping of the soil types present in Haskell County; second, a collection of the existing vegetation of each soil type; third, an identification of the vegetation collected and observed; fourth, and lastly, a comparative study of the vegetation of each soil type in an effort to establish a correlation between the vegetation and the soil type.