Use of luminescence energy transfer probes to detect genetic variants.
Description:
The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A …
more
Date:
August 2004
Creator:
Vaccaro, Carlos
Partner:
UNT Libraries