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  Partner: UNT Libraries
 Decade: 2010-2019
 Degree Level: Master's
 Collection: UNT Theses and Dissertations
A Preliminary Controller Design for Drone Carried Directional Communication System

A Preliminary Controller Design for Drone Carried Directional Communication System

Date: August 2015
Creator: AL-Emrani, Firas
Description: In this thesis, we conduct a preliminary study on the controller design for directional antenna devices carried by drones. The goal of the control system is to ensure the best alignment between two directional antennas so as to enhance the performance of air-to-air communication between the drones. The control system at the current stage relies on the information received from GPS devices. The control system includes two loops: velocity loop and position loop to suppress wind disturbances and to assure the alignment of two directional antennae. The simulation and animation of directional antennae alignment control for two-randomly moving drones was developed using SIMULINK. To facilitate RSSI-based antenna alignment control to be conducted in the future work, a study on initial scanning techniques is also included at the end of this thesis.
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My Whine, Your Wine

My Whine, Your Wine

Date: December 2011
Creator: Abbott, Shannon Marie
Description: Grapes hold the flavors of the lands where they grow, and when you make wine from them, those flavors of the land come through. Tasting wine from a place you've been can bring you back to that place with aromas and notes indicative of that place. A bottle of wine changes every day, and how it will taste depends on the moment you choose to release it from the glass walls. I have a vested interest in wine, because it is a living thing. I am compelled to make wine because its characteristics are like personality traits. Although some of those characteristics are harsh at times, I appreciate them all. Each trait plays an important role in the balance, the overall personality. Like my own personality flaws, wine's harsh tones can smooth over time. My relationship with wine is constantly evolving, with every new varietal, vintage, batch and blend. Believe me, after some of the jobs I had before my first day at Su Vino, I cherish every moment of my winemaking career. My Whine, Your Wine is the story of how it all started.
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Field Validation of Zero Energy Lab Water-to-Water Ground Coupled Heat Pump Model

Field Validation of Zero Energy Lab Water-to-Water Ground Coupled Heat Pump Model

Date: May 2016
Creator: Abdulameer, Saif
Description: Heat pumps are a vital part of each building for their role in keeping the space conditioned for the occupant. This study focuses on developing a model for the ground-source heat pump at the Zero Energy lab at the University of North Texas, and finding the minimum data required for generating the model. The literature includes many models with different approaches to determine the performance of the heat pump. Each method has its pros and cons. In this research the equation-fit method was used to generate a model based on the data collected from the field. Two experiments were conducted for the cooling mode: the first one at the beginning of the season and the second one at the peak of the season to cover all the operation conditions. The same procedure was followed for the heating mode. The models generated based on the collected data were validated against the experiment data. The error of the models was within ±10%. The study showed that the error could be reduced by 20% to 42% when using the field data to generate the model instead of the manufacturer’s catalog data. Also it was found that the minimum period to generate the cooling ...
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Jacques-Antoine-Hippolyte, comte de Guibert: Father of the Grande Armée

Jacques-Antoine-Hippolyte, comte de Guibert: Father of the Grande Armée

Date: May 2011
Creator: Abel, Jonathan, 1985-
Description: The eighteenth century was a time of intense upheaval in France. The death of Louis XIV in 1715 and the subsequent reign of Louis XV saw the end of French political and martial hegemony on the continent. While French culture and language remained dominant in Europe, Louis XV's disinterested rule and military stagnation led to the disastrous defeat of the French army at the hands of Frederick the Great of Prussia in the Seven Years War (1756-1763). The battle of Rossbach marked the nadir of the French army in the Seven Years War. Frederick's army routed the French infantry that had bumbled its way into massed Prussian cavalry. Following the war, two reformist elements emerged in the army. Reformers within the government, chiefly Etienne François, duc de Choiseul, sought to rectify the army's poor performance and reconstitute France's military establishment. Outside the traditional army structure, military thinkers looked to military theory to reinvigorate the army from within and without. Foremost among the latter was a young officer named Jacques-Antoine-Hippolyte de Guibert, whose 1772 Essai général de tactique quickly became the most celebrated work of theory in European military circles. The Essai provided a new military constitution for France, proposing wholesale ...
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Source Apportionment Analysis of Measured Volatile Organic Compounds in Corpus Christi, Texas

Source Apportionment Analysis of Measured Volatile Organic Compounds in Corpus Christi, Texas

Date: May 2014
Creator: Abood, Ahmed T.
Description: Corpus Christi among of the largest industrialized coastal urban areas in Texas. The strategic location of the city along the Gulf of Mexico allows for many important industries and an international business to be located. The cluster of industries and businesses in the region contribute to the air pollution from emissions that are harmful to the environment and to the people living in and visiting the area. Volatile organic compounds (VOC) constitute an important class of pollutants measured in the area. The automated gas chromatography (Auto GC) data was collected from Texas Commission of Environmental Quality (TCEQ) and source apportionment analysis was conducted on this data to identify key sources of VOC affecting this study region. EPA PMF 3.0 was employed in this sources apportionment study of measured VOC concentration during 2005 - 2012 in Corpus Christi, Texas. The study identified nine optimal factors (Source) that could explain the concentration of VOC at two urbane monitoring sites in the study region. Natural gas was found to be the largest contributor of VOC in the area, followed by gasoline and vehicular exhaust. Diesel was the third highest contributor with emissions from manufacturing and combustion processes. Refineries gases and evaporative fugitive emissions ...
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Development of a Cost Effective Wireless Sensor System for Indoor Air Quality Monitoring Applications

Development of a Cost Effective Wireless Sensor System for Indoor Air Quality Monitoring Applications

Date: May 2014
Creator: Abraham, Sherin
Description: Poor air quality can greatly affect the public health. Research studies indicate that indoor air can be more polluted than the outdoor air. An indoor air quality monitoring system will help to create an awareness of the quality of air inside which will eventually help in improving it. The objective of this research is to develop a low cost wireless sensor system for indoor air quality monitoring. The major cost reduction of the system is achieved by using low priced sensors. Interface circuits had to be designed to make these sensors more accurate. The system is capable of measuring carbon dioxide, carbon monoxide, ozone, temperature, humidity and volatile organic compounds. The prototype sensor node modules were developed. The sensor nodes were the connected together by Zigbee network. The nodes were developed in such a way that it is compact in size and wireless connection of sensor nodes enable to collect air quality data from multiple locations simultaneously. The collected data was stored in a computer. We employed linear least-square approach for the calibration of each sensor to derive a conversion formula for converting the sensor readings to engineering units. The system was tested with different pollutants and data collected was ...
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Ultrafast Laser Sampling of a Plant Tissue and ion Conductivity Measurement for Investigation of Light Stress Generation Mechanisms

Ultrafast Laser Sampling of a Plant Tissue and ion Conductivity Measurement for Investigation of Light Stress Generation Mechanisms

Date: August 2010
Creator: Abtahi, Seyed Ali
Description: In this study we applied ultra-short laser pulses on a biological sample (Arabidopsis), in order to cut it precisely in a square pattern and subsequently use it for studying stress generation mechanisms. For this purpose, we utilized femtosecond laser pulses at 100 fs pulse width and 80 MHz repetition rate. We took two processing parameters into consideration such as laser power, laser exposure time which is related to the stage speed. Therefore, we were able to find the laser optimum conditions for ablation of biological tissues. The mutant and wildtype (control) obtained from laser cutting with a size of 500 µm × 500 µm were directly transferred (in-situ with laser cutting) into a microfabricated chamber containing ~500 nanoliters deionized water for measuring ion conductivity. The ion conductivity is a signature of cell-death mechanisms caused by various stresses. A light with intensity of 100 µmol was exposed to the samples for 2 hours and 20 minutes as a source of stress. A quantitative electrical analysis with high accuracy was assured by utilizing a microchamber, which enables a measurement in nanoliter volume. We measured the impedance which is reciprocal of conductivity using a lock-in amplifier and a precise current source at frequency ...
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Integrating Selective Herbicide and Native Plant Restoration to Control Alternanthera philoxeroides (Alligator Weed)

Integrating Selective Herbicide and Native Plant Restoration to Control Alternanthera philoxeroides (Alligator Weed)

Date: December 2011
Creator: Adams, Justin
Description: Exotic invasive aquatic weeds such as alligator weed (Alternanthera philoxeroides) threaten native ecosystems by interfering with native plant communities, disrupting hydrology, and diminishing water quality. Development of new tools to combat invaders is important for the well being of these sensitive areas. Integrated pest management offers managers an approach that combines multiple control methods for better control than any one method used exclusively. In a greenhouse and field study, we tested the effects of selective herbicide application frequency, native competitor plant introduction, and their integration on alligator weed. In the greenhouse study, alligator weed shoot, root, and total biomass were reduced with one herbicide application, and further reduced with two. Alligator weed cumulative stem length and shoot/root ratio was only reduced after two applications. In the greenhouse, introduction of competitors did not affect alligator weed biomass, but did affect shoot/root ratio. The interaction of competitor introduction and herbicide did not significantly influence alligator weed growth in the greenhouse study. In the field, alligator weed cover was reduced after one herbicide application, but not significantly more after a second. Introduction of competitor species had no effect on alligator weed cover, nor did the interaction of competitor species and herbicide application. This ...
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Qos Aware Service Oriented Architecture

Qos Aware Service Oriented Architecture

Date: August 2013
Creator: Adepu, Sagarika
Description: Service-oriented architecture enables web services to operate in a loosely-coupled setting and provides an environment for dynamic discovery and use of services over a network using standards such as WSDL, SOAP, and UDDI. Web service has both functional and non-functional characteristics. This thesis work proposes to add QoS descriptions (non-functional properties) to WSDL and compose various services to form a business process. This composition of web services also considers QoS properties along with functional properties and the composed services can again be published as a new Web Service and can be part of any other composition using Composed WSDL.
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Determining the Emissivity of Roofing Samples: Asphalt, Ceramic and Coated Cedar

Determining the Emissivity of Roofing Samples: Asphalt, Ceramic and Coated Cedar

Date: December 2015
Creator: Adesanya, Oludamilola
Description: The goal is to perform heat measurements examine of selected roofing material samples. Those roofing materials are asphalt shingles, ceramics, and cedar. It’s important to understand the concept of heat transfer, which consists of conduction, convection, and radiation. Research work was reviewed on different infrared devices to see which one would be suitable for conducting my experiment. In this experiment, the main focus was on a specific property of radiation. That property is the emissivity, which is the amount of heat a material is able to radiate compared to a blackbody. An infrared measuring device, such as the infrared camera was used to determine the emissivity of each sample by using a measurement formula consisting of certain equations. These equations account for the emissivity, transmittance of heat through the atmosphere and temperatures of the samples, atmosphere and background. The experiment verifies how reasonable the data is compared to values in the emissivity table. A blackbody method such as electrical black tape was applied to help generate the correct data. With this data obtained, the emissivity was examined to understand what factors and parameters affect this property of the materials. This experiment was conducted using a suitable heat source to heat ...
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Knowledge Based System and Decision Making Methodologies in Materials Selection for Aircraft Cabin Metallic Structures

Knowledge Based System and Decision Making Methodologies in Materials Selection for Aircraft Cabin Metallic Structures

Date: August 2016
Creator: Adhikari, Pashupati Raj
Description: Materials selection processes have been the most important aspects in product design and development. Knowledge-based system (KBS) and some of the methodologies used in the materials selection for the design of aircraft cabin metallic structures are discussed. Overall aircraft weight reduction means substantially less fuel consumption. Part of the solution to this problem is to find a way to reduce overall weight of metallic structures inside the cabin. Among various methodologies of materials selection using Multi Criterion Decision Making (MCDM) techniques, a few of them are demonstrated with examples and the results are compared with those obtained using Ashby's approach in materials selection. Pre-defined constraint values, mainly mechanical properties, are employed as relevant attributes in the process. Aluminum alloys with high strength-to-weight ratio have been second-to-none in most of the aircraft parts manufacturing. Magnesium alloys that are much lighter in weight as alternatives to the Al-alloys currently in use in the structures are tested using the methodologies and ranked results are compared. Each material attribute considered in the design are categorized as benefit and non-benefit attribute. Using Ashby's approach, material indices that are required to be maximized for an optimum performance are determined, and materials are ranked based on the ...
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Solvent Effects and Bioconcentration Patterns of Antimicrobial Compounds in Wetland Plants

Solvent Effects and Bioconcentration Patterns of Antimicrobial Compounds in Wetland Plants

Date: May 2011
Creator: Adhikari, Sajag
Description: This study looked at effects of organic solvents dimethylsulfoxide, dimethylformamide and acetone at 0.01%, 0.05% and 0.1% concentration on germination and seedling development wetland plants. Even at 0.01% level, all solvents affected some aspect of seed germination or seedling growth. Acetone at 0.01% was least toxic. Root morphological characteristics were most sensitive compared to shoot morphological characteristics. This study also looked at bioconcentration patterns of antimicrobial compounds triclosan, triclocarban and methyl-triclosan in wetland plants exposed to Denton Municipal Waste Water Treatment Plant effluent. Bioconcentration patterns of antimicrobial compounds varied among species within groups as well as within organs of species. The highest triclocarban, triclosan and methyltriclosan concentration were in shoot of N. guadalupensis, root of N. lutea and in shoots of P. nodous respectively.
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Presence of Wolbachia, A Potential Biocontrol Agent: Screening for Vertebrate Blood Meal Source and West Nile Virus in Mosquitoes in the North Texas Region

Presence of Wolbachia, A Potential Biocontrol Agent: Screening for Vertebrate Blood Meal Source and West Nile Virus in Mosquitoes in the North Texas Region

Date: August 2016
Creator: Adiji, Olubu Adeoye
Description: West Nile virus (WNV) is a geographically endemic mosquito-borne flavivirus that has spread across the United States infecting birds, mosquitos, humans, horses and other mammals. The wide spread nature of this virus is due to the ability of the mosquito vector to persist in broad, ecological diverse environments across the United States. In this study, mosquito populations in North Texas region were sampled for detection of Wolbachia, blood meal source, and WNV. The ultimate goal of this study was to examine the potential of a biocontrol agent, Wolbachia sp. that colonizes the hindgut of various insects, including mosquitos, as a natural means to interrupt virus transmission from mosquitos to other hosts, including humans. In Australia, Wolbachia sp. from fruit flies (Drosophila melanogaster) have been successfully used to block transmission of a similar pathogenic virus from mosquitos responsible for transmission of Dengue fever. Here, mosquitoes were collected using CDC style Gravid Traps in Denton, Texas, from October 2012 through September 2014. Collected mosquitoes were identified, sexed, and categorized as to the amount of host blood in their alimentary system using a Zeiss Axio Zoom microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY). Culex quinquefaciatus was the dominant blood engorged species collected. Smaller ...
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A Study of the Drafting Curriculum in the Fort Worth Public High Schools, Fort Worth, Texas

A Study of the Drafting Curriculum in the Fort Worth Public High Schools, Fort Worth, Texas

Date: December 2012
Creator: Agee, David W.
Description: This is a study of the drafting curriculum in the Fort Worth public high schools, Fort Worth, Texas.The specific purposes or the study were as follows: 1. To review and compare the courses and content of the drafting curriculums listed in the bulletins of the TEA and the Fort Worth Public Schools. 2. To study drafting scheduling procedures in each of the Fort Worth public high schools. 3. To study the courses and contents of the drafting curriculums offered in each of the Fort Worth public high schools. 4. To determine if there are variations from school to school in curriculum content of the drafting courses in each of the Fort Worth public high schools. 5. To offer suggestions and recommendations for improving the program, if weaknesses were evident, when the program was evaluated by acceptable criteria (12, 13, and 14).
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Spatial Mismatch Between Hiv Infection and Access to Hiv Service Facilities in Texas

Spatial Mismatch Between Hiv Infection and Access to Hiv Service Facilities in Texas

Date: August 2013
Creator: Aggrey Korsah, Emmanuel
Description: Since 2004, the number of people living with HIV (PLWH) has steadily increased by about 5% and currently, the number in Texas is about 86,000. Though the National HIV/AIDS Strategic Plan seeks to ensure “unfettered access to quality healthcare”, barriers to access still exist especially among minority populations. This study examines the relationship between HIV infection rates and the geographic location of HIV service centers with a focus on 4 counties: namely, Dallas, Denton, Harris and Tarrant. The goal is to show whether there is a spatial mismatch between HIV rates and service providers. Are service facilities located in zip codes where they are most needed? Using the vulnerability framework and the Inverse Care Law (ICL), we address the research question using demographic variables (race/ethnicity, sex, poverty, education attainment) and HIV data. Our results show that extreme vulnerable zip codes have high HIV rates and closest proximity to HIV service providers.
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“No Paper Cowboys”: Stories

“No Paper Cowboys”: Stories

Date: December 2015
Creator: Agnew, Bryn
Description: Equilibrium is paramount in the crafting of a story, and for every writer this sense of balance is different. The writer must manage a balance of showing and telling, of denotation and connotation, and forever strive to find the perfect word in both the denotative and connotative sense, so that the reader and writer can meeting in a living story—both in the ink on the page and the remaining white space.
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Determinants of Effort and Associated Cardiovascular Response to a Behavioral Restraint Challenge

Determinants of Effort and Associated Cardiovascular Response to a Behavioral Restraint Challenge

Date: December 2015
Creator: Agtarap, Stephanie
Description: This study directly tested implications of motivation intensity theory on effort to restrain against a behavioral urge or impulse (i.e. restraint intensity). Two factors were manipulated—magnitude of an urge and the importance of successfully resisting it—with cardiovascular (CV) responses related to active coping measured. Male and female undergraduate students were presented with a mildly- or strongly evocative film clip with instructions to refrain from showing any facial response. Success was made more or less important through coordinated manipulations of outcome expectancy, ego-involvement, and performance assessment. As expected, systolic blood pressure responses assessed during the performance period were proportional to the evocativeness of the clip when importance was high, but low regardless of evocativeness when importance was low. These findings support a new conceptual analysis concerned with the determinants and CV correlates of restraint intensity. Implications of the study and associations with current self-regulatory literature are discussed.
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Family Structure and Marijuana Use Among Adolescents

Family Structure and Marijuana Use Among Adolescents

Date: May 2014
Creator: Aguilar, Jamie M.
Description: Family structure as a predictive variable of juvenile delinquency has been studied for the last hundred years. This relationship originated due to societal belief that divorce was detrimental to adolescents. Due to the changing societal roles in the United States, family structure has been changing. More children are growing up in non-intact families, such as single-parent households, households with stepparents, cohabitating families, and households without a parent present. To study the effect family structure has on juvenile delinquency, researchers have utilized social control theory, differential association, self-control theory and general strain theory to conceptualize variables to explain why family structure influences delinquent behavior. A review of previous literature on this topic indicates that living in intact households, which are households with two biological parents who are married, have, on average, the lowest rates of delinquency. This thesis investigates the relationship between family structure and lifetime marijuana use among eighth and tenth grade adolescents in the United States through the use of secondary data analysis of Monitoring the Future Study, 2012. The results provide support for the relationship between family structure and lifetime marijuana use.
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Interpersonal Factors Related to the Pursuit of a Higher Education Among First Generation Undergraduate Students

Interpersonal Factors Related to the Pursuit of a Higher Education Among First Generation Undergraduate Students

Date: May 2012
Creator: Aguirre, Jacqueline S.
Description: The typical educational stressors experienced by college students, in conjunction with developmental stressors such as separation from parents, individuation, and perceived social support, can interact to impact adaptation significantly. First generation college students (students who are the first in their family to attend college) can experience stressors beyond the typical educational stressors experienced by later generation college students, including lack of support from family and peers as well as financial difficulties that can interact to impact the pursuit of an education beyond the undergraduate level. The present study examined factors that may be especially influential in the pursuit of a higher education for first generation college students. Results indicated that aspects of family enmeshment were related to academic motivation for first generation students, but not for later generation students. Exploratory analysis showed that family and finances were mentioned more often among first generation students when compared to later generation students as stressors that strongly influence the desire to continue beyond the undergraduate level.
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Ozone Pollution of Shale Gas Activities in North Texas

Ozone Pollution of Shale Gas Activities in North Texas

Date: May 2016
Creator: Ahmadi, Mahdi
Description: The effect of shale gas activities on ground-level ozone pollution in the Dallas-Fort Worth area is studied in detail here. Ozone is a highly reactive species with harmful effects on human and environment. Shale gas development, or fracking, involves activities such as hydraulic fracturing, drilling, fluid mixing, and trucks idling that are sources of nitrogen oxides (NOX) and volatile organic compounds (VOC), two of the most important precursors of ozone. In this study two independent approaches have been applied in evaluating the influences on ozone concentrations. In the first approach, the influence of meteorology were removed from ozone time series through the application of Kolmogorov-Zurbenko low-pass filter, logarithmic transformation, and subsequent multi-linear regression. Ozone measurement data were acquired from Texas Commission on Environmental Quality (TCEQ) monitoring stations for 14 years. The comparison between ozone trends in non-shale gas region and shale gas region shows increasing ozone trends at the monitoring stations in close proximity to the Barnett Shale activities. In the second approach, the CAMx photochemical model was used to assess the sensitivity of ozone to the NOX and VOC sources associated with shale oil and gas activities. Brute force method was applied on Barnett Shale and Haynesville Shale emission ...
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Energy Harvesting Wireless Piezoelectric Resonant Force Sensor

Energy Harvesting Wireless Piezoelectric Resonant Force Sensor

Date: December 2013
Creator: Ahmadi, Mehdi
Description: The piezoelectric energy harvester has become a new powering option for some low-power electronic devices such as MEMS (Micro Electrical Mechanical System) sensors. Piezoelectric materials can collect the ambient vibrations energy and convert it to electrical energy. This thesis is intended to demonstrate the behavior of a piezoelectric energy harvester system at elevated temperature from room temperature up to 82°C, and compares the system’s performance using different piezoelectric materials. The systems are structured with a Lead Magnesium Niobate-Lead Titanate (PMN-PT) single crystal patch bonded to an aluminum cantilever beam, Lead Indium Niobate-Lead Magnesium Niobate-Lead Titanate (PIN-PMN-PT) single crystal patch bonded to an aluminum cantilever beam and a bimorph cantilever beam which is made of Lead Zirconate Titanate (PZT). The results of this experimental study show the effects of the temperature on the operation frequency and output power of the piezoelectric energy harvesting system. The harvested electrical energy has been stored in storage circuits including a battery. Then, the stored energy has been used to power up the other part of the system, a wireless resonator force sensor, which uses frequency conversion techniques to convert the sensor’s ultrasonic signal to a microwave signal in order to transmit the signal wirelessly.
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Low Leakage Asymmetric Stacked Sram Cell

Low Leakage Asymmetric Stacked Sram Cell

Date: May 2014
Creator: Ahrabi, Nina
Description: Memory is an important part of any digital processing system. On-chip SRAM can be found in various levels of the memory hierarchy in a processor and occupies a considerable area of the chip. Leakage is one of the challenges which shrinking of technology has introduced and the leakage of SRAM constitutes a substantial part of the total leakage power of the chip due to its large area and the fact that many of the cells are idle without any access. In this thesis, we introduce asymmetric SRAM cells using stacked transistors which reduce the leakage up to 26% while increasing the delay of the cell by only 1.2% while reducing the read noise margin of the cell by only 15.7%. We also investigate an asymmetric cell configuration in which increases the delay by 33% while reduces the leakage up to 30% and reducing the read noise margin by only 1.2% compared to a regular SRAM cell.
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Space in Space: Privacy Needs for Long-Duration Spaceflight

Space in Space: Privacy Needs for Long-Duration Spaceflight

Date: May 2014
Creator: Aiken, Jo
Description: Space exploration is a uniquely human activity. As humans continue to push the limits of exploring the unknown, they have sought knowledge supporting the sustenance of life in outer space. New technologies, advancements in medicine, and rethinking what it means to be a “community” will need to emerge to support life among the stars. Crews traveling beyond the Moon will rely on the development of new technologies to support the technological aspects of their missions as well as their quality of life while away from Earth. Likewise, through advancements in medicine, scientists will need to address remaining questions regarding the effects of long-duration spaceflight on the human body and crew performance. Space explorers must learn to utilize these new technologies and medical advancements while learning to adapt to their new environment in space and as a space community. It is important that researchers address these issues so that human survival beyond Earth is not only achievable but so that life among the stars is worth living and sustaining. This thesis addressed these issues in an attempt to extend the trajectory of space exploration to new horizons.
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Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle

Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle

Date: December 2010
Creator: Akel, Amal
Description: Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single quantum dots were photoactivated, localized and reduced to their centroid and then reconstructed to a super-resolution image. The acquired super-resolution image shows a lateral and an axial sub-diffraction resolution and demonstrates ultrafine striations with widths less than 70 nm that are not evident by conventional confocal microscopy. The striations appear to be related to nebulin thin filament binding protein. This newly developed imaging system is cutting edge for its high resolution and localization as well its simplicity and convenience.
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