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open access

Lactate Dehydrogenase of Hymenolepis Diminuta: Isolation and Characterization

Description: Lactate dehydrogenase was isolated in pure form from crude extract of the cestode Hymenoleois diminuta by heat treatment and column chromatography. The purified enzyme has a specific activity of 106 units per mg protein. The molecular weight of the purified protein was 75,000 as determined by Sephadex gel filtration and analytical ultracentrifugation. An equilibrium ultracentrifugation study suggests a subunit molecular weight of 39,000. From these data, a dimer form of the native enzyme is pro… more
Date: December 1971
Creator: Burke, William F.
Partner: UNT Libraries

Probabilistic Modeling for Whole Metagenome Profiling

Description: To address the shortcomings in existing Markov model implementations in handling large amount of metagenomic data with comparable or better accuracy in classification, we developed a new algorithm based on pseudo-count supplemented standard Markov model (SMM), which leverages the power of higher order models to more robustly classify reads at different taxonomic levels. Assessment on simulated metagenomic datasets demonstrated that overall SMM was more accurate in classifying reads to their res… more
Date: May 2021
Creator: Burks, David
Partner: UNT Libraries

Benefits of Probiotics on Mortality, Growth Performance, Physiological Condition and Gut Histomophology of Juvenile Red Drum (Sciaenops ocellatus)

Description: Results from the present study found for the first time that the use of bacterial strains of Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium thermophilum, and Enterococcus faecium from the commercial product PrimaLac® had the potential to act as a possible probiotic for juvenile red drum. The addition of PrimaLac® probiotics [whether as a water-soluble probiotic (WSP) or in a probiotic enhanced starter feed (PESF)] reduced mortality (%M), enhanced growth rates (MW, LT, SGR, and … more
Date: May 2021
Creator: Busby, Wren Adell
Partner: UNT Libraries
open access

Protein Profiles of Azotobacter Vinelandii During the Encystment Process

Description: Azotobacter vinelandii 12837 was grown in Burk's glucose media and transferred onto Burk's n-butanol agar plates to allow for the formation of cysts. The patterns of the vegetative cell proteins were compared for each successive day of cyst formation, using the polyacrylamide gel isoelectric focusing technique. The findings revealed that, as the cysts developed to maturity, definite changes occurred in the protein constitution, indicative of the biochemical and physiological changes which cells… more
Date: August 1977
Creator: Butler, Mark A.
Partner: UNT Libraries
open access

Effects of Nutrient Media on Growth and Morphology of Azotobacter Vinelandii

Description: The work described in this thesis was undertaken to study the reasons why Azotobacter vinelandii ATCC 12837 after incubation in Burk's nitrogen-free liquid will not form as many colonies when plated on Difco Tryptic Soy Agar as when planted on Burk's nitrogen-free agar. The difference in growth of A. vinelandii on the two agars was established by performing viable cell-plate counts. The difference in growth was most apparent at 24-hours incubation of the Burk's liquid-media cultures. Phase cont… more
Date: August 1974
Creator: Butsch, Robert W.
Partner: UNT Libraries
open access

The Production of Fragile Cysts by an Aberrant Strain of Azotobacter Chroococcum Isolated from Soil

Description: The purpose of this study is to determine if a strain of Azotobacter chroococcum isolated from the soil in northern Louisiana produces cysts which are as resistant to deleterious agents as those produced by previously reported strains os Azotobacter.
Date: May 1969
Creator: Cagle, Gerald D.
Partner: UNT Libraries
open access

Phenotypic Analysis of Medicago truncatula NPF1.7 Over-Expressing Plants Grown under Different Nitrate Conditions

Description: Plants have many nitrate transporters; in the model legume Medicago truncatula, MtNPF1.7 is among them. MtNPF1.7 is important for M. truncatula growth and it has been established that MtNPF1.7 is a high affinity nitrate transporter. M. truncatula plants with mutations in MtNPF1.7 gene show defects during plants growth, with striking abnormalities in nodule development and root architecture. Nitrogen fixation is an energy expensive process; when legumes have sufficient bioavailable nitrogen like… more
Date: December 2017
Creator: Cai, Jingya
Partner: UNT Libraries
open access

Geographic Variation in Chromosomes and Morphology of Peromyscus Maniculatus in Texas and Oklahoma

Description: This study was initiated after finding two chromosomal types of Peromyscus maniculatus north and south of the Red River in Texas and Oklahoma. The problem was to explain the chromosomal variations and their implications to the systematics of the grassland subspecies of P. maniculatus in this region.
Date: August 1972
Creator: Caire, William, 1946-
Partner: UNT Libraries
open access

Eutrophication Monitoring and Prediction

Description: Changes in trophic status are often related to increases or decreases in the allocthonous inputs of nutrients from changes in land use and management practices. Lake and reservoir managers are continually faced with the questions of what to monitor, how to monitor it, and how much change is necessary to be considered significant. This study is a compilation of four manuscripts, addressing one of these questions, using data from six reservoirs in Texas.
Date: December 1993
Creator: Cairns, Stefan H., 1949-
Partner: UNT Libraries
open access

The Roles of Genic Behavioral and Biochemical Mechanisms in the Adaption of Minnows of the Genus Notropis (Cyprinidae) to Temperature

Description: Electrophoretic variation at twenty gene loci, patterns of behavioral thermoregulation, and genotype-specific malate dehydrogenase kinetics were investigated among populations of the red shiner, Notropis lutrensis, and the blacktail shiner, N. venustus, collected from thermally altered and thermally unaltered portions of their ranges. Genic variation was found to be high among red shiners and low among blacktail shiners. The behavioral response of the blacktail shiner to temperature was fixed a… more
Date: December 1981
Creator: Calhoun, Stuart W. (Stuart Wayne)
Partner: UNT Libraries
open access

Shortened in Vivo Bioconcentration Factor Testing in Cyprinus Carpio

Description: Bioconcentration factor testing serves as the most valuable surrogate for the assessment of bioaccumulation. The assessment of potentially harmful chemicals is crucial to not only the health of aquatic environments, but to humans as well. Chemicals that possess the ability to persist in the environment or that have the potential to bioaccumulate, pose a greater risk to organisms that are exposed to these chemicals. The Organization for Economic Cooperation and Development Guideline 305 outlines… more
Date: December 2013
Creator: Cantu, Mark
Partner: UNT Libraries
open access

Phosphorus Metabolism in Atypical Mycobacteria

Description: The design for this study was tri-phasic: 1) to establish growth time patterns for each group of atypical mycobacteria, 2) to demonstrate the dynamic state of phosphorus in the various fractions by determining its incorporation and turnover, 3) to determine quantitatively the amount of phosphorus in each fraction.
Date: August 1966
Creator: Carnes, James E.
Partner: UNT Libraries
open access

In Vivo and In Vitro Transformations of Mouse Tissues from a Murine Lymphosarcoma

Description: The problem with which this investigation is concerned is that of determining the nature of events leading to the change. of normal cells into malignant cells. The design of the study is multi-phasic: (A) to establish the presence or absence of an oncogenic virion, (B) to demonstrate by use of the electron microscopy any ultracellular alteration in malignant or transformed tissues, (C) to investigate the nature of the transforming agent in the murine lymphosarcoma, and (D) to employ various met… more
Date: August 1972
Creator: Carnes, James Edgar
Partner: UNT Libraries
open access

Opthalmic Use Of Sodium Cephalothin: An In Vivo Comparison

Description: A rabbit keratoconjunctivities model was used to evaluate ophthalmic formulations containing 1 percent sodium cephalothin in silicon oil, a 1 percent sodium cephalothin aqueous solution, and a 0.3 percent gentamicin sulfate solution. Rabit eyes were inoculated intracorneally with Pseudomonas aeruginosa, Staphylococcus aureus, or Streptococcus pneumoniae, After topical treatment, none of the antibiotic formulations were effective in the P. aeruginosa model; all three showed good activity agains… more
Date: August 1979
Creator: Carney, Gerald R.
Partner: UNT Libraries

Biogeography of Montane Mammals on the Colorado Plateau and Adjacent Regions

Description: This study identifies the biogeographic factors that structure small mammal communities on mountains of the Colorado Plateau and adjacent regions. Forty six isolated ranges were characterized across a 5-state study area encompassing the Colorado Plateau, including the central high plateaus of Utah and the Basin and Range Province (i.e. the Great Basin and mountains of Arizona and New Mexico). Presence/absence data of 25 montane mammal species were used to explore the interactions between histor… more
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Date: May 2004
Creator: Carr, Carla B.
Partner: UNT Libraries
open access

Temperature Tolerance of Freshwater Fish Exposed to Water-Borne Cadmium

Description: Lethal toxicity values (96 h LC50; mg Cd/L) for the test species were similar: Lepomis cyanellus, 11.52; Notropis lutrensis, 6.62; Pimephales promelus, 3.58. However the effects of cadmium concentration and exposure time on temperature tolerance varied between species. Neither cadmium concentration nor exposure time had a significant effect on the CTM of green sunfish. Both cadmium concentration and exposure time had a significant effect on the CTMs of red shiners and fathead minnows. By day 10… more
Date: May 1987
Creator: Carrier, Roxie
Partner: UNT Libraries
open access

Effects of Sertraline Exposure on Fathead Minnow (Pimephales promelas) Steroidogenesis

Description: Sertraline is a selective serotonin reuptake inhibitor (SSRI) that is widely used for the treatment of depression and anxiety. Due to the abundant therapeutic use of sertraline, low levels have been detected in municipal wastewater effluents suggesting that aquatic organisms may be exposed. The purpose of this study was to evaluate the steroidogenic effects of sertraline on larval (FHM) and adult female fathead minnows (FFHM), Pimephales promelas. Larval FHM were exposed to 0.1, 1, and 10 µg/L … more
Date: December 2014
Creator: Carty, Dennis R.
Partner: UNT Libraries
open access

A Study on the Serological Relationships of Various Fractions of Pseudomonas aeruginosa

Description: The purpose of this research was to determine the relationship of the slime layer antigen(s) to the "101" or LPS antigens and to attempt to evaluate the role of antibodies against the latter in protection against experimental infections in mice with the homologous strain of Pseudomonas aeruginosa. Results from agglutination tests, chromatographic separations, passive protection tests, and characterizations of the antigens by gel double diffusion do not support the concept that LPS is a necessar… more
Date: December 1973
Creator: Cash, Howard A.
Partner: UNT Libraries
open access

Use of Remote Sensing for Cover Type Interpretation Over the Ray Roberts Reservoir Area

Description: As part of a pre-impoundment study for the Ray Roberts Reservoir Area, Landsat-5 multi-spectral scanner (MSS) imagery was used for cover type interpretation. This research was concerned with analysis techniques for MSS images and a comparison of results obtained using computer assisted interpretatin of MSS images and a comparison of results obtained using computer assisted interpretation of MSS images with those obtained using manual interpretation of false color infrared (CIR) photographs.
Date: December 1987
Creator: Cassidy, Kelly Michela
Partner: UNT Libraries
open access

Niche Structure of an Anole Community in a Tropical Rain Forest within the Choco Region of Colombia

Description: Ten species of anoles at Bajo Calima within the Choco of Western Colombia separate into two principal microhabitat groups: forest species, and those inhabiting openings and edges. The ten anoles further separate according to ground and vegetation dwellers. There is a relation at Bajo Calima between the number of anole species and vegetational structural diversity. Anole diversity within a given macrohabitat is by perch microsite/microclimate heterogeneity. These are the two major ecological dim… more
Date: May 1988
Creator: Castro-Herrera, Fernando
Partner: UNT Libraries
open access

Taxonomy of the Azotobacteraceae

Description: The classification of the Azotobacteraceae to the level of genus and species has been uncertain since the studies of Beijerinck in 1901. This dissertation represents an effort to establish a system of classification more complete than the one now in use. In this study, both qualitative and quantitative taxonomy were used in order to establish a well founded classification scheme. Qualitative methods included certain important morphological and physiological characteristics, isoenzyme patterns, … more
Date: December 1980
Creator: Chang, Charles Shing
Partner: UNT Libraries
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