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open access

Degradation of Phenolic Acids by Azotobacter Species Isolated from Sorghum Fields

Description: Sorghum plants excrete phenolic acids which reduce subsequent crop yields. These acids accumulate in field soil by combining with soil and clay particles to form stable complexes which remain until degraded by bacterial metabolism. The amount of phenolic acids in soil samples were obtained by gas chromatography measurements, while Azotobacter populations were obtained by plate counts in 40 sorghum field samples from Denton County, Texas. One can conclude that increasing the Azotobacter populati… more
Date: August 1989
Creator: Al-Hadhrami, Mohamed N. (Mohamed Nasser)
Partner: UNT Libraries
open access

Site Directed Mutagenesis of Dienelactone Hydrolase

Description: The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.
Date: August 1995
Creator: Al-Khatib, Haifa Yousef
Partner: UNT Libraries
open access

The Physiology of Azotobacter Vinelandii Cysts

Description: The value of the adenylate energy charge [(ATP)+1/2(ADP)/(ATP)+(ADP)+(AMP)] in Azotobacter vinelandii cells was monitored during growth and germination in flask cultures. The miximal value of 0.88 was attained during mid-log phase; this declined gradually to 0.50 by late stationary phase. When these cultures were transferred to encystment media, the adenylate energy charge decreased to an average value of 0.40 as the vegetative cells encysted and remained unchanged during the next 20 days. Ency… more
Date: December 1980
Creator: Aladegbami, Solomon L.
Partner: UNT Libraries
open access

Effects of Phytohormones on Scenedesmus quadricauda

Description: The literature on the effects of phytohormone on algae is clouded with contradictory reports. Reports have been published which substantiate and deny the effects of phytohormones in enhancing the growth and developmental processes in algae. The overall aim of this study was to investigate the response, if any, of the phytohormones indole-3-acetic acid (IAA), gibberellic acid A3 (GA) and kinetin on the physiology of the green alga, Scenedesmus quadricauda. Results obtained for the uptake of 14^C… more
Date: August 1977
Creator: Alam, Mohammad Ihtisham
Partner: UNT Libraries

Novel Approaches for Enhancing Resistance to Fusarium graminearum in Arabidopsis and Wheat by Targeting Defense and Pathogenicity Factors

Description: Fusarium head blight (FHB) is an important disease of small grain cereals including wheat that affects grain quality and yield. The fungus Fusarium graminearum (Fg) is the major agent of this disease. Lack of natural resistance has limited ability to control wheat losses to this disease. Developing new approaches is critical for increasing host plant resistance to this fungus. This work has identified four processes that can be targeted for enhancing host plant resistance to FHB. The first i… more
Date: May 2020
Creator: Alam, Syeda Tamanna
Partner: UNT Libraries
open access

Assessing Student Perceptions in Short Research Experiences and Course Research Experiences in Undergraduate Biology Laboratories

Description: This study examined students' perception between short research experiences (SRE) courses and full-semester course research experiences (CRE) using the Persistence in the Sciences (PITS) survey and the interview questionnaire. The study also aimed to correlate the influence of student's demographic as a predictive indicator for Project Ownership Scores (POS) and Quantitative Literacy (QL) score means. The three courses studied at the University of North Texas were Biology for Science Majors Lab… more
Date: August 2022
Creator: Alberts, Arland Dulcey
Partner: UNT Libraries
open access

Long-Term Citizen Science Water Monitoring Data: An Exploration of Accuracy over Space and Time

Description: The Texas Stream Team (TST) is one of an increasing number of citizen science water monitoring programs throughout the US which have been continuously collecting surface water quality data under quality assurance protocols for decades. Volunteer monitoring efforts have generated monitoring datasets that are long-term, continuous, and cover a large geographic area - characteristics shown to be valuable for scientists and professional agencies. However, citizen science data has been of limited u… more
Date: December 2018
Creator: Albus, Kelly
Partner: UNT Libraries
open access

Transient Expression of BABY BOOM, WUSCHEL, and SHOOT MERISTEMLESS from Virus-Based Vectors in Cotton Explants: Can We Accelerate Somatic Embryogenesis to Improve Transformation Efficiency?

Description: Upland cotton (Gossypium hirsutum L.) is the world's most prominent fiber crop. Cotton transformation is labor intensive and time consuming, taking 12 to 18 months for rooted T0 plants. One rate limiting step is the necessary production of somatic embryos. In other recalcitrant species, ectopic expression of three genes were shown to promote somatic embryogenesis: WUSCHEL (WUS), SHOOT MERISTEMLESS (STM), and BABY BOOM (BBM). WUS is responsible for maintaining stem-cell fate in shoot and floral … more
Date: December 2019
Creator: Alejos, Marcos
Partner: UNT Libraries
open access

Exploration of Explanatory Variables in the Creation of Linear Regression Models and Logistic Regression Models to Predict the Performance of Preservice Teachers on the Science Portion of the EC-6 TExES Certification Examination

Description: The purpose of this study was to analyze the current and pre-service conditions that can affect student teachers' preparedness to pass the science portion of the EC-6 Texas Examinations for Educator Standards (TExES), one of the mandatory certification exam to become a teacher in Texas. Two types of prediction models were employed in this study: binomial logistic regression and multiple linear regression. The independent variables used in this study were: final grade in BIOL 1082, classificatio… more
Date: December 2019
Creator: Alexis, Naudin
Partner: UNT Libraries
open access

Role of 5.8S rRNA in Zebrafish and Human Blood Coagulation

Description: Hemolytic disorders are characterized by hemolysis and are prone to thrombosis. Previously, it has been shown that the RNA released from damaged blood cells activates clotting. However, the nature of RNA released from hemolysis is still elusive. We found that after hemolysis, the red blood cells from both zebrafish and humans release 5.8S rRNA. This RNA activated coagulation in zebrafish and human plasmas. Using both natural and synthetic 5.8S rRNA and its synthetic truncated fragments, we foun… more
Date: December 2020
Creator: Alharbi, Abdulmajeed Haya M.
Partner: UNT Libraries
open access

A Genetic Approach to Identify Proteins that Interact with Eukaryotic Microtubule Severing Proteins via a Yeast Two Hybrid System

Description: Microtubules (MT) are regulated by multiple categories of proteins, including proteins responsible for severing MTs that are therefore called MT-severing proteins. Studies of katanin, spastin, and fidgetin in animal systems have clarified that these proteins are MT-severing. However, studies in plants have been limited to katanin p60, and little is known about spastin or fidgetin and their function in plants. I looked at plant genomes to identify MT-severing protein homologues to clarify which … more
Date: May 2020
Creator: Alhassan, Hassan H
Partner: UNT Libraries
open access

The Role of the Actin Cytoskeleton in Asymmetric Cell Division in Maize

Description: Stomata are specialized plant structures required for gaseous exchange with the outer environment. During stomata formation, the cytoskeleton plays an important role in controlling the division of the individual cells leading to the generation of the stomata complex. Two mutants that affect microfilament and microtubule organization in subsidiary mother cells include brk1 and dcd1. While only 20% of the subsidiary cells in the brk1 and dcd1 single mutants are abnormally shaped, it was reported… more
Date: August 2014
Creator: Alhassan, Hassan Hamdan
Partner: UNT Libraries
open access

A Behavioral Model for Detection of Acute Stress in Bivalves

Description: A behavioral model for acute responses in bivalves, was developed using time series analysis for use in a real-time biomonitoring unit. Stressed bivalves closed their shell and waited for the stressful conditions to pass. Baseline data showed that group behavior of fifteen bivalves was periodic, however, individuals behaved independently. Group behavior did not change over a period of 20 minutes more than 30 percent, however, following toxic exposures the group behavior changed by more than 30 … more
Date: May 1998
Creator: Allen, H. Joel
Partner: UNT Libraries
open access

Evaluation of Diet, Water, and Culture Size for Ceriodaphnia Dubia Laboratory Culturing

Description: Six reagent waters, eleven diets, and two culture sizes were evaluated for culturing C. dubia. Different filtration techniques were used to prepare the reagent waters. The eleven diets were comprised of two algae augmented with eight supplements. Reproduction and growth were assessed to discern differences among C. dubia raised in mass cultures and cultured in individual cups, during which, bacterial population densities, lipid, protein, and carbohydrate concentrations of the diets were measure… more
Date: December 1992
Creator: Allen, Jerry D. (Jerry Dee)
Partner: UNT Libraries
open access

Effects of Suspended Multi-Walled Carbon Nanotubes on Daphnid Growth and Reproduction

Description: Multi-walled carbon nanotube aggregates can be suspended in the aqueous phase by natural organic matter. These aggregates are ingested by filter feeding zooplankton. Ingested aggregates result in decreased growth and decreased reproduction. These effects may be caused by reduction in energy input from normal feeding behavior. pH alters natural organic matter structure through changes in electrostatic repulsion. Altered natural organic matter structure changes multi-walled carbon nanotube aggreg… more
Date: May 2010
Creator: Alloy, Matthew Michael
Partner: UNT Libraries

Analysis of Multipartite Bacterial Genomes Using Alignment-Free and Alignment-Based Pipelines

Description: In this work, we have performed comparative evolutionary analysis, functional genomics analysis, and machine learning analysis to identify the molecular factors that discriminate between multipartite and unipartite bacteria, with the goal to decipher taxon-specific factors and those that are prevalent across the taxa underlying the these traits. We assessed the roles of evolutionary mechanisms, namely, horizontal gene transfer and gene gain, in driving the divergence of bacteria with single and… more
Date: August 2021
Creator: Almalki, Fatemah
Partner: UNT Libraries
open access

The Generation of Recombinant Zea mays Spastin and Katanin Proteins for In Vitro Analysis

Description: Plant microtubules play essential roles in cell processes such as cell division, cell elongation, and organelle organization. Microtubules are arranged in highly dynamic and ordered arrays, but unlike animal cells, plant cells lack centrosomes. Therefore, microtubule nucleation and organization are governed by microtubule-associated proteins, including a microtubule-severing protein, katanin. Mutant analysis and in vitro characterization has shown that the highly conserved katanin is needed fo… more
Date: December 2017
Creator: Alodailah, Sattam Sonitan
Partner: UNT Libraries
open access

Novel Role of Trypsin in Zebrafish

Description: It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved in thrombocyte activation via PAR2 during gill bleeding. In this study, I investigated another role of the trypsin that is secreted from zebrafish. This investigation has demonstrated a novel role of trypsin in zebrafish. Not only did this investigation demonstrate the role of trypsin in zebrafish behavior, but also it showed that PAR2 might be … more
Date: May 2013
Creator: Alsrhani, Abdullah Falleh
Partner: UNT Libraries
open access

Studies in Trypsin as an Alarm Substance in Zebrafish

Description: Previous studies have shown that fish release alarming substances into the water to alert their kin to escape from danger. In our laboratory, we found that zebrafish produce trypsin and release it from their gills into the environment when they are under stress. By placing the zebrafish larvae in the middle of a small tank and then placing trypsin at one end of the tank, we observed that the larvae moved away from the trypsin zone and almost to the opposite end of the tank. This escape response… more
Date: August 2018
Creator: Alsrhani, Abdullah Falleh
Partner: UNT Libraries
open access

Hooking Mortality of Largemouth Bass Caught on Controversial Artificial Lures and Live Bait : Lake Ray Roberts, Texas

Description: A total of 192 largemouth bass were caught at Lake Ray Roberts, Texas (1995) to investigate five controversial bass angling techniques relative to hooking mortality. The bait types were Texas-rigged scented and non-scented plastic worms, Carolina-rigged scented and non-scented plastic worms, and live golden shiners. Overall hooking mortality was 21.87% and mortality was dependent upon bait type. Highest mortality resulted from the Texas-rigged scented lures, while the lowest mortality was gener… more
Date: May 1996
Creator: Alumbaugh, Andrew E. (Andrew Edward)
Partner: UNT Libraries
open access

Retinoic acid Treatment Affects Kidney Development and Osmoregulatory System in the Developing Chicken (Gallus Gallus)

Description: Development is a dynamic process characterized by critical periods in which organ systems are sensitive to changes in the surrounding environment. In the current study, critical windows of embryonic growth and kidney development were assessed in the embryonic chicken. All‐trans retinoic acid (tRA) influences not only organogenesis and cell proliferation, but also targets metanephric kidney nephrogenesis. Embryonic chickens were given a single injection of tRA on embryonic day 8. tRA decre… more
Date: May 2011
Creator: Alvine, Travis Douglas
Partner: UNT Libraries
open access

Isolation and Bioinformatic Characterization of Four Novel Bacteriophages from Streptomyces toxytricini

Description: Six initial phage isolates with high titer lysates were obtained using Streptomyces toxytricini B-5426 as the host bacterium. These isolates were named Goby, Toma, Yosif, Yara, Deema, and Hsoos. However, upon completion of the sequencing, it was found that the Yara and Hsoos isolates were identical, as were Goby and Deema. As a result, final analysis was completed on only the four unique isolates. All of the phages mentioned above were isolated from soil samples from different locations. Al… more
Date: May 2019
Creator: Alzaid, Hessah
Partner: UNT Libraries

Quantifying the Effects of Single Nucleotide Changes in the TATA Box of the Cauliflower Mosaic Virus 35S Promoter on Gene Expression in Arabidopsis thaliana

Description: Synthetic biology is a rapidly growing field that aims to treat cellular biological networks in an analogous way to electrical circuits. However, the field of plant synthetic biology has not grown at the same pace as bacterial and yeast synthetic biology, leaving a dearth of characterized tools for the community. Due to the need for tools for the synthetic plant biologist, I have endeavored to create a library of well-characterized TATA box variants in the cauliflower mosaic virus (CaMV) 35S pr… more
Date: December 2021
Creator: Amack, Stephanie Carolina
Partner: UNT Libraries
open access

Investigation of Strategies for Improving STR Typing of Degraded and Low Copy DNA from Human Skeletal Remains and Bloodstains

Description: Forensic STR analysis is limited by the quality and quantity of DNA. Significant damage or alteration to the molecular structure of DNA by depurination, crosslinking, base modification, and strand breakage can impact typing success. Two methods that could potentially improve STR typing of challenged samples were explored: an in vitro DNA repair assay (PreCR™ Repair Mix) and whole genome amplification. Results with the repair assay showed trends of improved performance of STR profiling of blea… more
Date: August 2014
Creator: Ambers, Angie D.
Partner: UNT Libraries
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