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  Partner: UNT Libraries
 Department: Department of Biology
 Degree Discipline: Biology
 Collection: UNT Theses and Dissertations
Impact of a Genetically Engineered Probiotic Therapy and Igf-1 Genomics in the Pahenu2 Mouse Model of Pku

Impact of a Genetically Engineered Probiotic Therapy and Igf-1 Genomics in the Pahenu2 Mouse Model of Pku

Date: December 2015
Creator: Durrer, Katherine Elaine
Description: Absence of functional phenylalanine hydroxylase results in phenylketonuria (PKU). Viable treatments remain few, expensive and secondary conditions such as osteopenia occur in most PKU patients. Objective 1: Given the recently described roles of gut microbes to aid host digestion, an orally administered genetically engineered probiotic as the delivery vehicle for enzyme replacement therapy was created. The engineered probiotic, pHENOMMenal, produced phenylalanine ammonia lyase with significant production of trans-cinnamate (phenylalanine cleavage product) in vitro and resulted in a reduction of 515 μM in blood phenylalanine when fed to PKU animals for 14 days (from 2307µM ± 264µM to 1792µM ± 261µM, n = 6, P < 0.05). The control probiotic produced no change in blood phenylalanine. Thus, pHENOMMenal treatment in PKU mice demonstrated engineered microbes could compensate for a metabolic deficiency of the host. Objective 2: Evaluate the PAHenu2 mouse model of PKU for a genetic discrepancy causing ocular enlargement and delayed development observed only after the PAHenu2 mutation was crossed to the C57BL/6J mouse. When compared to healthy littermates, ELISA indicated a consistent but insignificant decrease in plasma IGF-1 and an increase in ocular IGF-1 in PKU animals. SNP screening demonstrated a differential inheritance of IGF-1 alleles in healthy and ...
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The Impact of Developmental Stress on Cardiovascular Physiology of Two Archosaur Species: American Alligator (Alligator mississippiensis) and Domestic Chicken (Gallus gallus)

The Impact of Developmental Stress on Cardiovascular Physiology of Two Archosaur Species: American Alligator (Alligator mississippiensis) and Domestic Chicken (Gallus gallus)

Date: December 2014
Creator: Tate, Kevin B.
Description: Crocodilians and birds comprise sister taxa of archosaurs, the development of these vertebrates occurs within an egg case that leaves developing embryos susceptible to fluctuations in the nesting environment. Studies suggest that sub-optimal conditions alter morphological growth and cardiovascular physiology. Regulation of the cardiovascular system is immature in the subjects studied, and embryos may rely on humoral rather than neural control of the cardiovascular system. The primary focus of this dissertation was to assess regulatory mechanisms responsible for maintenance of arterial pressure and heart rate. Dehydration stress had marked effects on embryo growth, and altered baseline cardiovascular parameters, while leaving the response to humoral regulator, angiotensin II (Ang II), unaffected. However, dehydrated alligator embryos developed cholinergic tone on heart rate. Hypoxic incubated chicken embryos were reduced in embryo mass, and altered response to humoral regulatory components Ang I and adenosine in addition identifying a novel regulatory component of the cardiovascular response to acute hypoxia. Collectively, these studies add to the existing knowledge of cardiovascular physiology in embryonic archosaurs and suggest that some components of cardiovascular regulation are plastic following developmental stress.
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Microflora in Prepared Foods Dispensed from Eating Establishments in Dallas, Texas

Microflora in Prepared Foods Dispensed from Eating Establishments in Dallas, Texas

Date: August 1948
Creator: Phillips, Margaret
Description: A bacteriological study was made of a number of prepared foods that were bought ready-to-eat, for home consumption, from several food establishments in Dallas, Texas. The purpose of this study was to show whether these foods could be a potential source of food poisoning; whether there are any particular foods that should have greater care than others in order to protect their quality; and whether the bacteriological contamination could have been prevented by strict observance of the city regulations for handling foods.
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A Study of Selected Factors Influencing Science Majors Toward Science Careers

A Study of Selected Factors Influencing Science Majors Toward Science Careers

Date: August 1957
Creator: Bragg, Louis Hairston
Description: "The purpose of this study is to gather information concerning some factors which may have operated to influence science majors in North Texas State College toward science careers."--4.
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The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes

The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes

Date: August 1959
Creator: Sissom, Stanley L.
Description: The role that aquatic actinomycetes play, in the production of tastes and odors in water supplies has been investigated since 1948. The ability of these organisms to produce by-products in lakes and streams, which renders the water unpalatable, is of considerable public health importance. It is desirable that the waterworks industry has as much information as possible concerning the factors that contribute to the growth of these organisms. Since it appears that the aquatic actinomycetes may be isolated from most fresh-water sources, the problem of diversified environments and nutritional requirements offers an excellent field of investigation. The fresh-waters of the world contain variable quantities of electrolytes that may determine in part the biological activity of these organisms. The unsolved questions in this instance are concerned with the electrolytes present and their quantitative effects on the growth and development of these forms.
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A Study of a Selected Group of Science Related Characteristics of Non-Science Majors

A Study of a Selected Group of Science Related Characteristics of Non-Science Majors

Date: August 1957
Creator: Bearden, Bennie Paul
Description: The purpose of this study is to gather information related to the characteristics of two groups of undergraduate non-science majors; namely, those students who once considered science as a career and subsequently changed to a non-science major and those who have never considered a career in science. It is frequently observed that children in the elementary school have an intense interest in science. One of the principal concerns of this study is how and for what reasons has the interest in science changed between elementary school and college? It is also the purpose of this study to gather information related to the attitudes of these college students toward the science courses they have had in high school and toward their science teachers. In attempting to arrive at an answer, the group who once considered a science career and those who have never considered an occupational choice in a field of science will be compared in the following areas: 1. General information such as sex, age, size of high school attended, rank within their graduating classes, college classification and military experiences. 2. Occupations and hobbies of parents. 3. Experiences which may influence attitudes toward science such as elementary school science, high ...
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Bacteriological Studies of the Campus Drinking Fountains of North Texas State Teachers College Denton, Texas

Bacteriological Studies of the Campus Drinking Fountains of North Texas State Teachers College Denton, Texas

Date: August 1941
Creator: McCoy, Eloise
Description: "In order to gain an adequate idea of the sanitary condition of the drinking fountains on the North Texas State Teachers College campus, it was found necessary to approach these bacteriological studies from a seasonal point of view."--1.
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A Vegetative Key to the Grasses of Erath County, Texas

A Vegetative Key to the Grasses of Erath County, Texas

Date: August 1951
Creator: Robinson, Inez Evans
Description: "The orientation of this study began with a collection of the grasses of Erath County during their flowering periods. Collections were made in the Spring and Fall of 1950 and 1951 to obtain both vernal and autumnal grasses. Identifications were made from the grass floras previously mentioned and checked against specimens in the herbarium of North Texas State College for verification. In addition, the list was checked by W.A. Turney, Conservationist, United States Department of Agriculture of Dublin, Texas. Finally, the list was compared with that prepared by W.M. Cory for the plant areas of which Erath County is a part. On the basis of the above identifications, a critical study of the vegetative characteristics was made and a key based on these features prepared. The width of the blad and characteristics of the ligule, roots, culms, and sheaths were used to distinguish each of the eighty-six specimens found in the collection area. A drawing was made of the longitudinal section of the juncture of the blade and the sheath of each grass. This diagnostic section includes the collar, ligule, a part of the blade and sheath, and auricles, if present."-- leaf 5.
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An Investigation of Naturally Occurring Tastes and Odors from Fresh Waters

An Investigation of Naturally Occurring Tastes and Odors from Fresh Waters

Date: May 1955
Creator: Pipes, Wesley O'Feral, Jr.
Description: The Problem of the cause and control of tastes and odors in water supplies has been the subject of a great many investigations during the last half-century; however, many questions in this field remain to be answered. Theories relating to the cause of tastes and odors occurring in water are still rather numerous and, at present, only a few of them are compatible. Although several procedures have been employed for the eradication of tastes and odors from water supplies, none of them are completely successful.
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The Effects of Hypothermia on the Release of Cardiac Enzymes

The Effects of Hypothermia on the Release of Cardiac Enzymes

Date: August 1977
Creator: Strawn, William B.
Description: The myocardium is known to release CPK, LDH1 , and GOT in response to ischemia as a result of myocardial infarction. This study was designed to induce the release of cardiac enzymes without adversely effecting the myocardium by perfusion hypothermia, thereby suggesting that these enzymes are not as specific in the diagnosis of myocardial infarction as once thought. Hypothermia was by in vivo perfusion of the left anterior descending coronary artery. Enzyme activity was measured from sera samples spectrophotometrically and electrophoretically. Significant CPK and LDH1 increases were observed in animals perfused between 25 and 19 C. These results indicate that, while heart function remained unchanged, an alteration occurred in the membrane integrity of the myocardial cells.
Contributing Partner: UNT Libraries
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