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 Decade: 2010-2019
 Collection: UNT Theses and Dissertations
Identification of Three Symbiosome Targeting Domains in the MtENOD8 Protein and Cell-to-cell MtENOD8 mRNA Movement in Nodules

Identification of Three Symbiosome Targeting Domains in the MtENOD8 Protein and Cell-to-cell MtENOD8 mRNA Movement in Nodules

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Date: May 2012
Creator: Meckfessel, Matthew Harold
Description: The model legume, Medicago truncatula, is able to enter into a symbiotic relationship with soil bacteria, known as rhizobia. This relationship involves a carbon for nitrogen exchange in which the plant provides reduced carbon from photosynthesis in exchange for reduced, or “fixed” atmospheric nitrogen, which allows the plant to thrive in nitrogen depleted soils. Rhizobia infect and enter plant root organs, known as nodules, where they reside inside the plant cell in a novel organelle, known as the symbiosome where nitrogen fixation occurs. the symbiosome is enriched in plant proteins, however, little is known about the mechanisms that direct plant proteins to the symbiosome. Using the M. truncatula ENOD8 (MtENOD8) protein as a model to explore symbiosome protein targeting, 3-cis domains were identified within MtENOD8 capable of directing green fluorescent protein (GFP) to the symbiosome, including its N-terminal signal peptide (SP). the SP delivered GFP to the vacuole in the absence of nodules suggesting that symbiosome proteins share a common targeting pathway with vacuolar proteins. a time course analysis during nodulation indicated that there is a nodule specific redirection of MtENOD8-SP from the vacuole to the symbiosome in a MtNIP/LATD dependent manner. GFP expression by the MtENOD8 promoter revealed spatial ...
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Incorporating Electrochemistry and X-ray Diffraction Experiments Into an Undergraduate Instrumental Analysis Course

Incorporating Electrochemistry and X-ray Diffraction Experiments Into an Undergraduate Instrumental Analysis Course

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Date: May 2012
Creator: Molina, Cathy
Description: Experiments were designed for an undergraduate instrumental analysis laboratory course, two in X-ray diffraction and two in electrochemistry. Those techniques were chosen due their underrepresentation in the Journal of Chemical Education. Paint samples (experiment 1) and pennies (experiment 2) were characterized using x-ray diffraction to teach students how to identify different metals and compounds in a sample. in the third experiment, copper from a penny was used to perform stripping analyses at different deposition times. As the deposition time increases, the current of the stripping peak also increases. the area under the stripping peak gives the number of coulombs passed, which allows students to calculate the mass of copper deposited on the electrode surface. the fourth experiment was on the effects of variable scan rates on a chemical system. This type of experiment gives valuable mechanistic information about the chemical system being studied.
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Interspecimen Study of Bone to Relate Macromechanical, Nanomechanical and Compositional Changes Across the Femoral Cortex of Bone

Interspecimen Study of Bone to Relate Macromechanical, Nanomechanical and Compositional Changes Across the Femoral Cortex of Bone

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Date: May 2013
Creator: Nar, Mangesh
Description: Mechanics of bone is widely studied and researched, mainly for the study of fracture. This has been done mostly on a macro scale. In this work hierarchical nature of bone has been explored to investigate bone mechanics in more detail. Flexural test were done to classify the bones according to their strength and deflection. Raman spectroscopy analysis was done to map the mineralization, collagen crosslinking changes across the thickness of the bone. Nanoindentation was done to map indentation hardness and indentation modulus across femoral cortex of the bone. The results indicate that the composition of the bone changes across the thickness of the femoral cortex. The hypothesis is confirmed as increase in mineralization, carbonate to phosphate ratio and collagen crosslinking shows the effect as increased indentation hardness and modulus and decreased deflection.
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Investigating Postpartum Depression in Southern Rural Egypt and Effects of Sertraline on Fsh and Lh Gene Expression on Fathead Minnows Using Rt-pcr

Investigating Postpartum Depression in Southern Rural Egypt and Effects of Sertraline on Fsh and Lh Gene Expression on Fathead Minnows Using Rt-pcr

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Date: May 2013
Creator: Mohamed, Hagar Abdo
Description: Postpartum depression (PPD) is a major health problem that affects many women worldwide. In Egypt, PPD is neglected despite the expected high prevalence rate among women during the transition period after the Egyptian revolution. This research investigated the prevalence, risk factors, and interventions of postpartum depression in southern rural Egypt. Interviews were conducted with 57 participants recruited from public and private hospitals. Questionnaires and the Arabic version of the Edinburgh Postnatal Depression Scale were administered. The prevalence of PPD is 73.7%. PPD is associated with low income and age at childbirth. Most participants regarded screening mothers after childbirth for PPD as effective; in comparison to, antidepressants that were regarded by most participants as ineffective. Women in southern rural Egypt prefer high number of pregnancies, so investigating the influence of sertraline, an antidepressant medication, on female hormones becomes important. In this research, fathead minnows were exposed to 3 and 10 ppb sertraline for 7 days. Real-time Polymerase Chain Reaction was used to detect the change in gene expression of the Follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Results showed that a down regulation at the 10 ppb was evident on the LH and to a lesser extent on FSH. Our results ...
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Investigation of Novel Electrochemical Synthesis of Bioapatites and Use in Elemental Bone Analysis

Investigation of Novel Electrochemical Synthesis of Bioapatites and Use in Elemental Bone Analysis

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Date: December 2012
Creator: DeLeon, Vallerie H.
Description: In this research, electrochemical methods are used to synthesize the inorganic fraction of bone, hydroxyapatite, for application in biological implants and as a calibration material for elemental analysis in human bone. Optimal conditions of electrochemically deposited uniform apatite coatings on stainless steel were investigated. Apatite is a ceramic with many different phases and compositions that have beneficial characteristics for biomedical applications. Of those phases hydroxyapatite (HA) is the most biocompatible and is the primary constituent of the inorganic material in bones. HA coatings on metals and metal alloys have the ability to bridge the growth between human tissues and implant interface, where the metal provides the strength and HA provides the needed bioactivity. The calcium apatites were electrochemically deposited using a modified simulated body fluid adjusted to pH 4-10, for 1-3 hours at varying temperature of 25-65°C while maintaining cathodic potentials of -1.0 to -1.5V. It was observed that the composition and morphology of HA coatings change during deposition by the concentration of counter ions in solution, pH, temperature, applied potential, and post-sintering. The coatings were characterized by powder x-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. The precipitated powders from the experiment were also characterized, with results ...
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Irony, Humor, and Ontological Relationality in Literature

Irony, Humor, and Ontological Relationality in Literature

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Date: August 2012
Creator: Kim, Soon Bae
Description: The purpose of this dissertation is to investigate ontological relationality in literary theory and criticism by critically reflecting on modern theories of literature and by practically examining the literary texts of Geoffrey Chaucer, William Shakespeare, and Oscar Wilde. Traditional studies of literary texts have been oriented toward interpretative or hermeneutic methodologies, focusing on an independent and individual subject in literature. Instead, I explore how relational ontology uncovers the interactive structures interposed between the author, the text, and the audience by examining the system of how the author's creative positioning provokes the reader's reaction through the text. In Chapter I, I critically inquire into modern literary theories of "irony" in Romanticism, New Criticism, and Deconstructionism to show how they tend to disregard the dynamic dimension of interactive relationships between different literary subjects. Chapter II scrutinizes Wilde's humor in An Ideal Husband (1895) and The Importance of Being Earnest (1895) in order to reveal the ontological relationships triggered by a creative positioning. In chapter III, I examine Chaucer's The Canterbury Tales (c. 1400) and the laughter in "The Miller's Tale" in particular, to examine the ethical and aesthetic dimensions of its interactive relationships. In Chapter IV, I explore Much Ado About Nothing ...
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Jeans, Boots, and Starry Skies: Tales of a Gay Country-and-Western Bar and Places Nearby

Jeans, Boots, and Starry Skies: Tales of a Gay Country-and-Western Bar and Places Nearby

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Date: May 2010
Creator: Gay, Wayne Lee
Description: Fourteen short stories, with five interspersed vignettes, describe the lives of gay people in the southwestern United States, centered around a fictional gay country-and-western bar in Dallas and a small town in Oklahoma. Various characters, themes, and trajectories recur in the manner of a short story cycle, as explained in the prefatory Critical Analysis, which focuses on exemplary works of James Joyce, Ernest Hemingway, William Faulkner, Shirley Jackson, Italo Calvino, Yevgeny Kharitonov, and Louise Erdrich.
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The Key to Unlocking the Secret Window

The Key to Unlocking the Secret Window

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Date: December 2010
Creator: McConnell, Sarah E.
Description: David Koepp's Secret Window was released by Columbia Pictures in 2004. The film's score was written by Philip Glass and Geoff Zanelli. This thesis analyzes transcriptions from six scenes within the film in conjunction with movie stills from those scenes in an attempt to explain how the film score functions.
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The Klezmer Influence in Paul Schoenfield’s Klezmer Rondos

The Klezmer Influence in Paul Schoenfield’s Klezmer Rondos

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Date: December 2011
Creator: Trimble, Mark
Description: Paul Schoenfield’s Klezmer Rondos is a work for flute, male vocalist, and orchestra revised in 1994 according to the score given to me by the composer. A review of current research in klezmer heritage music is the starting point to place Klezmer Rondos in the context of art music infused with klezmer flavor. Klezmer music can be defined as the instrumental folk music of Eastern European Jews, however because of its adaptability and quality of assimilating other cultures within it, this heritage music is constantly in flux. By looking at the research in this field, I describe how the sound of klezmer music has evolved and how popular notions have been formed. The body of this research explores the main musical aspects of Klezmer Rondos that can be tied to the klezmer tradition: scales and thematic materials, improvisatory elements, ornamentation, and instrumentation. Klezmer Rondos moves beyond a simple arrangement of vernacular music for orchestra; it is a fusion of contemporary art music with the elements of klezmer style.
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The Legacy of Theodore Leschetizky as Seen through His Pedagogical Repertoire and Teaching Style

The Legacy of Theodore Leschetizky as Seen through His Pedagogical Repertoire and Teaching Style

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Date: May 2010
Creator: Serrin, Bret
Description: Theodore Leschetizky's singular pianistic legacy survives to this day because of his revolutionary pedagogical methods and his compositions for the piano repertory. The amalgamation of these two aspects formed his distinctive contributions to the fields of piano and piano pedagogy and left an indelible mark on the history of the instrument. His students lead an impressive list of the greatest artists of the previous century, each influencing the evolution of pianism with their own remarkable style and personality. While Leschetizky was arguably without peer as a pedagogue, many pianists today are unaware of the vast number of compositions that he wrote. These pieces were intended not only for the concert stage, but also as a very specific pedagogical repertoire that he used within his own teaching studio. This repertoire comprises a vital component of the Leschetizky legacy, albeit one which is often slighted in comparison. It is imperative that the pianists of our current generation understand the dual aspects of his contribution to our art form, in order to fully grasp the way in which he has changed the face of pianism. The purpose of this dissertation and lecture recital is to enumerate the various aspects that constitute the dual ...
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