Description: The activity of plant 9-lipoxygenases (LOXs) influences the outcome of Arabidopsis thaliana interaction with pathogen and insects. Evidence provided here indicates that in Arabidopsis, 9-LOXs facilitate infestation by Myzus persicae, commonly known as the green peach aphid (GPA), a sap-sucking insect, and infection by the fungal pathogen Fusarium graminearum. in comparison to the wild-type plant, lox5 mutants, which are deficient in a 9-lipoxygenase, GPA population was smaller and the insect spent less time feeding from sieve elements and xylem, thus resulting in reduced water content and fecundity of GPA. LOX5 expression is induced rapidly in roots of GPA-infested plants. This increase in LOX5 expression is paralleled by an increase in LOX5-synthesized oxylipins in the root and petiole exudates of GPA-infested plants. Micrografting experiments demonstrated that GPA population size was smaller on plants in which the roots were of the lox5 mutant genotype. Exogenous treatment of lox5 mutant roots with 9-hydroxyoctadecanoic acid restored water content and population size of GPA on lox5 mutants. Together, these results suggest that LOX5 genotype in roots is critical for facilitating insect infestation of Arabidopsis. in Arabidopsis, 9-LOX function is also required for facilitating infection by F. graminearum, which is a leading cause of Fusarium head ...

Description: As vehicle manufacturers continue to increase their emphasis on safety with advanced driver assistance systems (ADAS), I propose a ubiquitous device that is able to analyze and advise on safety conditions. Mobile smartphones are increasing in popularity among younger generations with an estimated 64% of 25-34 year olds already using one in their daily lives. with over 10 million car accidents reported in the United States each year, car manufacturers have shifted their focus of a passive approach (airbags) to more active by adding features associated with ADAS (lane departure warnings). However, vehicles manufactured with these sensors are not economically priced while older vehicles might only have passive safety features. Given its accessibility and portability, I target a mobile smartphone as a device to compliment ADAS that can bring a driver assist to any vehicle without regards for any on-vehicle communication system requirements. I use the 3-axis accelerometer of multiple Android based smartphone to record and analyze various safety factors which can influence a driver while operating a vehicle. These influences with respect to the driver, vehicle and road are lane change maneuvers, vehicular comfort and road conditions. Each factor could potentially be hazardous to the health of the driver, ...

Description: The model legume, Medicago truncatula, is able to enter into a symbiotic relationship with soil bacteria, known as rhizobia. This relationship involves a carbon for nitrogen exchange in which the plant provides reduced carbon from photosynthesis in exchange for reduced, or “fixed” atmospheric nitrogen, which allows the plant to thrive in nitrogen depleted soils. Rhizobia infect and enter plant root organs, known as nodules, where they reside inside the plant cell in a novel organelle, known as the symbiosome where nitrogen fixation occurs. the symbiosome is enriched in plant proteins, however, little is known about the mechanisms that direct plant proteins to the symbiosome. Using the M. truncatula ENOD8 (MtENOD8) protein as a model to explore symbiosome protein targeting, 3-cis domains were identified within MtENOD8 capable of directing green fluorescent protein (GFP) to the symbiosome, including its N-terminal signal peptide (SP). the SP delivered GFP to the vacuole in the absence of nodules suggesting that symbiosome proteins share a common targeting pathway with vacuolar proteins. a time course analysis during nodulation indicated that there is a nodule specific redirection of MtENOD8-SP from the vacuole to the symbiosome in a MtNIP/LATD dependent manner. GFP expression by the MtENOD8 promoter revealed spatial ...

Description: From the earliest civilizations, victorious armies would loot defeated cities or nations. the practice evolved into art theft as a symbol of power. Cultural superiority confirmed a country or empire’s regime. Throughout history, the Greeks and Romans cultivated, Napoleon Bonaparte refined, and Adolf Hitler perfected the practice of plunder. As the tides of Second World War began to shift in favor of the Allied Powers, special commissions, established to locate the Germans’ hoards of treasure, discovered Nazi art repositories filled with art objects looted from throughout Europe. the Soviet Union Trophy Commission and the Western Allies Monuments, Fine Arts, and Archives Commission competed to discover Nazi war loot. the two organizations not only approached the subject of plunder as a treasure hunt, but the ideology motivating both commissions made uncovering the depositories first, a priority. the Soviet trophy brigades’ mission was to dismantle all items of financial worth and ship them eastward to help rebuild a devastated Soviet economy. the Soviet Union wished for the re-compensation of cultural valuables destroyed by the Nazis’ purification practices regarding “inferior” Slavic art and architecture; however, the defeated German nation did not have the ability to reimburse the Soviet State. the trophy brigades implemented ...

Description: This dissertation explores Goodbye, Columbus and Five Short Stories (1959), the Ghost Writer (1979), the Counterlife (1986), the Facts (1988), Operation Shylock (1993), Sabbath's Theater (1995),and the Human Stain (2000), arguing that Roth relishes the telling of the story and the search for self within that telling. with attention to narrative technique and its relation to issues surrounding reality and identity, Roth's narratives stress unreliability, causing Roth to create characters searching for a more complex interpretation of self. Chapter I examines Roth’s negotiation of dual identities as Neil Klugman in Goodbye, Columbus feels alienated and displaced from Christianized America. the search for identity and the merging of American Christianity and Judaism remain a focus in Chapter II, which explores the implications of how, in the Ghost Writer, a young Nathan Zuckerman visits his mentor E.I. Lonoff to find him living in what he believes to be a non-Jewish environment—the American wilderness. Chapter II also examines the difficulties of cultural assimilation in "Eli, the Fanatic," in which Eli must shed outward appearances of Judaism to fit into the mostly Protestant community of Woodenton. Relative to the negotiation of multiple identities, Chapter III considers Sabbath’s attempt, in Sabbath’s Theater, to reconcile his ...