UNT Theses and Dissertations - 831 Matching Results

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Analysis of the Cytochrome P450 and UDP-Glucuronosyltransferase Families and Vitamin D3- Supplementation in Anoxia Survival in Caenorhabditis elegans

Description: Alteration in diet and knockdown of detoxification genes impacts the response of C. elegans to oxygen deprivation stress. I hypothesized that feeding worms a vitamin D3-supplementation diet would result in differential oxygen deprivation stress response. We used a combination of wet lab and transcriptomics approach to investigate the effect of a vitamin-D3 supplemented diet on the global gene expression changes and the anoxia response phenotype of C. elegans (Chapter 2). C. elegans genome consi… more
Date: December 2020
Creator: Agarwal, Sujata
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Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Description: The expression of two cotton osmotin genes was evaluated in terms of the mRNA and protein expression patterns in response to chemical inducers such as ethylene, hydrogen peroxide, and sodium chloride. Reverse transcriptase-polymerase chain reactions (RT-PCR) indicated that osmotin mRNAs are expressed constitutively in root tissues of cotton plants, and that they are rapidly induced in leaf and stem tissues upon ethylene treatment. Real time RT-PCR indicated that osmotin transcript levels were… more
Date: May 2007
Creator: Spradling, Kimberly Diane
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Analysis of the One-Horned Rhinoceros (Rhinoceros Unicornis) Habitat in the Royal Chitwan National Park, Nepal.

Description: This study analyzes the remaining suitable habitat of the one-horned rhinoceros, Rhinoceros unicornis, in Royal Chitwan National Park of Nepal. An April 2003 Landsat image was classified into eight land cover types: wetland, sand, water, mixed forest, sal forest, agriculture, settlement, and grassland. This image was converted into habitat suitability maps using cover, food, and water. The rhinoceros prefers grassland habitat with oxbow lakes and closed canopy during the monsoon season. Nomina… more
Date: December 2005
Creator: Thapa, Vivek
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Analysis of the Trypanosoma brucei Genome and Identification and Characterization of a Gene Family Encoding Putative EF-Hand Calcium-Binding Proteins

Description: The flagellum of Trypanosoma brucei contains a family of antigenically related EF-hand calcium-binding proteins which are called the calflagins. Genomic Southern blots indicated that multiple copies of calflagin genes occur in T brucei. All of the copies were contained in a single 23 kb Xhol-Xhol fragment. Genomic fragments of 2.5 and 1.7 kb were cloned that encoded calflagin sequences. Two new members of the calflagin family were found from genomic clone sequences. The deduced amino acid seque… more
Date: May 1998
Creator: DeFord, James H. (James Henry), 1956-
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Anatomical and Morphological Responses of Cardiospermum Halicacabum L. (Balloon Vine), to Four Levels of Water Availability

Description: C. halicacabum (Sapindaceae) is an invasive plant that is considered a nuisance species in Texas riparian environments. Little is known of the tolerance of C. halicacabum to flooding and drought; however, this information may provide insight into the characteristics that contribute to C. halicacabum purported invasiveness. C. halicacabum seedlings (n = 92) were exposed to one of four levels of water availability (flooded, saturated, intermediate and dry) over six weeks under greenhouse conditio… more
Date: May 2011
Creator: Dempsey, Matthew Anthony
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Anti-S2 Peptides and Antibodies Binding Effect on Myosin S2 and Anti-S2 Peptide's Ability to Reach the Cardiomyocytes in vivo and Interfere in Muscle Contraction

Description: The anti-S2 peptides, the stabilizer and destabilizer, were designed to target myosin sub-fragment 2 (S2) in muscle. When the peptides are coupled to a heart-targeting molecule, they can reach the cardiomyocytes and interfere with cardiac muscle contraction. Monoclonal antibodies, MF20 and MF30, are also known to interact with light meromyosin and S2 respectively. The MF30 antibody compared to anti-S2 peptides and the MF20 antibody is used as a control to test the central hypothesis that: Both … more
Date: July 2023
Creator: Quedan, Duaa Mohamad Alhaj Mahmoud
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Antimicrobial and Anti-Platelet Activity in Botanical Extracts of Plants Collected in Northern Thailand

Description: The purpose of my research work was to assess a variety of Northern Thailand plants tissue extracts for antibacterial and anti-platelet aggregation activity. The Minimum Inhibitory Concentration assay method was used to assess antimicrobial activity of plant extracts, while the Zebrafish Platelet Aggregation Assay and the in vitro Whole Human Blood Impedance Aggregation Assay were used to study anti-platelet activity. Forty one plant extracts harvested from the tissues of 26 plants collected fr… more
Date: May 2019
Creator: Patcharapinyopong, Thanasan
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Application of Cultured Neuronal Networks for Use as Biological Sensors in Water Toxicology and Lipid Signaling.

Description: This dissertation research explored the capabilities of neuronal networks grown on substrate integrated microelectrode arrays in vitro to be applied to toxicological research and lipid signaling. Chapter 1 details the effects of chlorine on neuronal network spontaneous electrical activity and pharmacological sensitivity. This study demonstrates that neuronal networks can maintain baseline spontaneous activity, and respond normally to pharmacological manipulations in the present of three times… more
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Date: August 2004
Creator: Dian, Emese Emöke
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Applications of Molecular Genetics to Human Identity.

Description: The primary objectives of this project were: 1. to develop improved methods for extraction of DNA from human skeletal remains, 2. to improve STR profiling success of low-copy DNA samples by employing whole genome amplification to amplify the total pool of DNA prior to STR analysis, and 3. to improve STR profiling success of damaged DNA templates by using DNA repair enzymes to reduce the number/severity of lesions that interfere with STR profiling. The data from this study support the following … more
Date: December 2008
Creator: Turnbough, Meredith A.
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Applications of Remote Sensing and GIS to Modeling Fire for Vegetative Restoration in Northern Arizona

Description: An accurate fire model is a useful tool in predicting the behavior of a prescribed fire. Simulation of fire requires an extensive amount of data and can be accomplished best using GIS applications. This paper demonstrates integrative procedures of using of ArcGIS™, ERDAS Imagine™, GPS, and FARSITE© to predict prescribed fire behavior on the Kaibab-Paiute Reservation. ArcGIS was used to create a database incorporating all variables into a common spatial reference system and format for the FARSIT… more
Date: August 2003
Creator: Hardison, Tanya
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Aquatic Heterotrophic Bacteria Active in the Biotransformation of Anthracene and Pentachlorophenol

Description: Dominant genera of bacteria were isolated from three river waters during anthracene and pentachlorophenol biotransformation studies. The genera Pseudomonas, Acinetobacter, Micrococcus, Chromobacterium, Alcaligenes, Azomonos, Bacillus, and Flavobacterium were capable of biotransforming one or both of these compounds. These isolates were subjected to further biotransformation tests, including river water and a basal salt medium with and without additional glucose. The results of these experiments… more
Date: August 1985
Creator: Entezami, Azam A. (Azam Alsadat)
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Aspartate Transcarbamoylase of Aeromonas Hydrophila

Description: This study focused on the enzyme, aspartate transcarbamoylase (ATCase) from A. hydrophila, a Gram-negative bacterium found in fresh water. The molecular mass of the ATCase holoenzyme from A. hydrophila is 310 kDa. The enzyme is likely composed of 6 catalytic polypeptides of 34 kDa each and 6 regulatory polypeptides of 17 kDa each. The velocity-substrate curve for A. hydrophila ATCase is sigmoidal for both aspartate and carbamoylphosphate. The Km for aspartate was the highest to date for an ente… more
Date: December 2000
Creator: Higginbotham, Leah
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Aspects of the Thermal Ecology of Largemouth Bass (Micropterus salmoides) in North Central Texas

Description: The coefficient of body temperature change (K) ranged from -0.53 to -0.072 for bass weighing 73-1440 g. The double log regression of K on weight was similar to that reported for other poikilotherms (slope = -0.57; R = 0.93). Fingerling bass were eurythermal, being capable of surviving instantaneous temperature changes over a 20 C range at acclimation temperatures of 15, 25 and 30 C and over a 15 C range at acclimation temperatures of 20 and 35 C. Preferred temperatures for adult bass measured i… more
Date: December 1976
Creator: Venables, Barney J.
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An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa

Description: The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the iso… more
Date: May 1978
Creator: Christensen, John N.
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Assembly of Pseudomonas putida Aspartate Transcarbamoylase and Possible Roles of the PyrC' Polypeptide in the Folding of the Dodecameric Enzyme

Description: Aspartate transcarbamoylase (ATCase) of Pseudomonas putida consists of two different polypeptides, PyrB and PyrC' (Schurr et al, 1995). The role of the PyrC' and the assembly of PyrB and PyrC' have been studied. The ATCase made in vitro of P.putida PyrB with P.putida PyrC', and of E.coli PyrB with P.putida PyrC ' were generated under two different conditions, denaturation and renaturation, and untreated. It was found that PyrC' plays a role in the enzymatic regulation by ATP, CTP and UTP. In … more
Date: May 1999
Creator: Hongsthong, Apiradee, 1970-
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Assessing Effects of an Environmental Education Field Science Program Fostering Responsibility at an Urban Middle School

Description: The study investigated the ability of an extracurricular program to influence environmental responsibility of sixth and seventh graders. The Children's Environmental Attitude and Knowledge Survey (CHEAKS) was evaluated for appropriateness in assessing the worth of this particular environmental education strategy emphasizing water quality fieldwork and technology. CHEAKS is designed with psychometric reliability and validity that may be used in comparing disparate programs. … more
Date: May 1999
Creator: Sills, Blake
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Assessing Outcomes of a Recycling Education and Service Program within an Elementary School

Description: During the spring 2004 a pilot school recycling program was implemented within Robert E. Lee Elementary. The primary goal of the program was to determine how recycling education in the school would affect curbside recycling rates within the surrounding community. The program was a cooperative effort between the University of North Texas, City of Denton Solid Waste Department and Keep Denton Beautiful. Throughout the first months of the study during the spring 2004, an increase in curbside rec… more
Date: August 2005
Creator: Cunningham-Scott, Carey Beth
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Assessing Regional Gully Erosion Risk: A Remote Sensing and Geographic Information Systems Approach

Description: Gully erosion has been established as a major source of sediment pollution in the upper Trinity River watershed in north-central Texas. This fact, along with a lack of models appropriate for a large-area gully erosion analysis established a need for a gully erosion study in the upper Trinity basin. This thesis project attempted to address this need by deriving an index indicative of gully erosion risk using remote sensing and geographic information systems (GIS) methodology. In context of previ… more
Date: August 1995
Creator: Sill, Paul E. (Paul Eric)
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Assessing Student Perceptions in Short Research Experiences and Course Research Experiences in Undergraduate Biology Laboratories

Description: This study examined students' perception between short research experiences (SRE) courses and full-semester course research experiences (CRE) using the Persistence in the Sciences (PITS) survey and the interview questionnaire. The study also aimed to correlate the influence of student's demographic as a predictive indicator for Project Ownership Scores (POS) and Quantitative Literacy (QL) score means. The three courses studied at the University of North Texas were Biology for Science Majors Lab… more
Date: August 2022
Creator: Alberts, Arland Dulcey
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Assessing the Effects of a Municipal Wastewater Treatment Plant Effluent on Zooplankton, Phytoplankton and Corbicula Flumina in a Constructed Wetland

Description: Wetland wastewater treatment offers low-cost, energy efficient alternatives to conventional wastewater technologies. In this study, an artificial wetland was constructed at the City of Denton, Texas Pecan Creek Water Reclamation Plant to facilitate diazinon removal from treated effluent.
Date: May 1995
Creator: Hymel, Stephanie Ramick
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Assessing the Spatial and Temporal Distribution of MTBE and BTEX Compounds in Lake Lewisville, Texas February 1999 - February 2000

Description: The spatial and temporal distribution of Methyl Tertiary-Butyl Ether (MTBE) and BTEX (Benzene, Toluene, Ethylbenzene, Xylenes) compounds were assessed in a multipurpose reservoir, Lake Lewisville, Texas between February 1999 and February 2000. Concentrations of MTBE ranged from 0.0 - 16.7 mg/L. Levels of MTBE in the lake were related to watercraft. BTEX concentrations were never detected above 2.0 mg/L during the sampling period. Finished drinking water from Denton and the Upper Trinity Regio… more
Date: August 2000
Creator: Lee, Anne W.
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An Assessment of Storm Water Toxicity from the Dallas/Fort Worth Metroplex and Denton, Texas

Description: With the advent of national storm water regulations, municipalities with populations greater than 100,000 are required to obtain National Pollutant Discharge Elimination System Permits (NPDES) for storm water discharges. In addition to the sampling required for the permit process, the City of Fort Worth contracted with the University of North Texas' Institute of Applied Sciences to conduct acute toxicity testing using Pimephales prcmelas and Ceriodaphnia dubia on storm water samples received fr… more
Date: August 1995
Creator: Keating, Paul Redmond
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An Assessment of the Use of Seeding, Mowing, and Burning in the Restoration of an Oldfield to Tallgrass Prairie in Lewisville, Texas

Description: An examination of the effectiveness of seeding, burning, and mowing in the reestablishment of tallgrass prairie species on overgrazed and abandoned pastureland. The study site is a 20 acre tract on U.S. Corps of Engineers land below Lake Lewisville in Denton County, Texas. The site was partitioned into thirty-nine 40 by 40 meter plots with seeding (carried out in 1996) and management treatment (burning, mowing, and no maintenance carried out in 1998) randomly applied following a two level desig… more
Date: August 1999
Creator: Windhager, Steven
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Attenuation of Escherichia Coli Aspartate Transcarbamoylase Expressed in Pseudomonas Aeruginosa Mutant and Wild Type Strains

Description: No apparent repression of pyr gene expression in Pseudomonas aeruginosa is observed upon addition of exogenous pyrimidines to the growth medium. Upon introduction of the subcloned Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) into a P. aeruginosa pyrB mutant strain, repression was observed in response to exogenously fed pyrimidine compounds. The results proved that it is possible to bring about changes in pyrimidine nucleotide pool levels and changes in transcriptional r… more
Date: December 1994
Creator: Liu, Haiyan, 1966-
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