An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa
Description:
The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the iso…
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Date:
May 1978
Creator:
Christensen, John N.
Partner:
UNT Libraries