| Description: | Campylobacter organisms are the most commonly reported bacterial causes of foodborne infection in the world, with Campylobacter jejuni and Campylobacter coli responsible for over 99% of reported infections. Traditionally, Campylobacter species detection is an arduous process, requiring a special incubation environment as well as specific growth media for an extended growth period. The development of a rapid and reliable diagnostic tool for the detection of Campylobacter species would be a valuable aid to the medical diagnostic decision process, especially to rule out Campylobacter infection during the enteric pre-surgical time period. Improved patient outcomes would result if this rapid assay could reduce the number of enteric surgeries. Assays performed during this dissertation project have demonstrated that both SYBR® green and hydrolysis probe assays targeting an 84 nucleotide portion of cadF, a fibronectin-binding gene of Campylobacter jejuni and Campylobacter coli, were able to detect from 101 to 108 copies of organism from stool specimens, did not detect nonspecific targets, and exhibited a coefficient of variation (CV) of 1.1% or less. Analytical validation of sensitivity, specificity and precision, successfully performed in these studies, warrants additional clinical validation of these assays. |
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| Creator(s): | Lewis, Sally |
| Creation Date: | May 2009 |
| Partner(s): |
UNT Libraries
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| Collection(s): |
UNT Theses and Dissertations
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| Usage: |
Total Uses: 412
Past 30 days: 15
Yesterday: 0
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| Publisher Info: |
Publisher Name: University of North Texas
Place of Publication: Denton, Texas
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| Original Creation Date: | May 2009 | |
| Description: | Campylobacter organisms are the most commonly reported bacterial causes of foodborne infection in the world, with Campylobacter jejuni and Campylobacter coli responsible for over 99% of reported infections. Traditionally, Campylobacter species detection is an arduous process, requiring a special incubation environment as well as specific growth media for an extended growth period. The development of a rapid and reliable diagnostic tool for the detection of Campylobacter species would be a valuable aid to the medical diagnostic decision process, especially to rule out Campylobacter infection during the enteric pre-surgical time period. Improved patient outcomes would result if this rapid assay could reduce the number of enteric surgeries. Assays performed during this dissertation project have demonstrated that both SYBR® green and hydrolysis probe assays targeting an 84 nucleotide portion of cadF, a fibronectin-binding gene of Campylobacter jejuni and Campylobacter coli, were able to detect from 101 to 108 copies of organism from stool specimens, did not detect nonspecific targets, and exhibited a coefficient of variation (CV) of 1.1% or less. Analytical validation of sensitivity, specificity and precision, successfully performed in these studies, warrants additional clinical validation of these assays. |
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| Degree: |
Name:
Doctor of Philosophy
Level:
Doctoral
Discipline:
Molecular Biology
Department:
Department of Biological Sciences
Grantor:
University of North Texas
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| Keyword(s): | PCR | Campylobacter | Real-time | |
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| Partner: |
UNT Libraries
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| Collection: |
UNT Theses and Dissertations
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| Identifier: |
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| Resource Type: | Thesis or Dissertation | |
| Format: | Text | |
| Rights: |
Access:
Public
License:
Copyright
Holder:
Lewis, Sally
Statement:
Copyright is held by the author, unless otherwise noted. All rights reserved.
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