A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

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Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.

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13 p. : col. ill.

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Dasgupta, Kasturi; Ganesan, Savita; Manivasagam, Sindhu & Ayre, Brian G. April 15, 2011.

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This article is part of the collection entitled: UNT Scholarly Works and was provided by the UNT College of Arts and Sciences to the UNT Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 718 times, with 4 in the last month. More information about this article can be viewed below.

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Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.

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13 p. : col. ill.

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Abstract: Background: Cytochrome P450 monooxygenases form a large superfamily of enzymes that catalyze diverse reactions. The P450su1 gene from the soil bacteria Streptomyces griseolus encodes CYP105A1 which acts on various substrates including sulfonylurea herbicides, vitamin D, coumarins, and based on the work presented here, brassinosteroids. P450su1 is used as a negative-selection marker in plants because CYP105A1 converts the relatively benign sulfonyl urea pro-herbicide R7402 into a highly phytotoxic product. Consistent with its use for negative selection, transgenic Arabidopsis plants were generated with P450su1 situated between recognition sequences for FLP recombinase from yeast to select for recombinase-mediated excision. However, unexpected and prominent developmental aberrations resembling those described for mutants defective in brassinosteroid signaling were observed in many of the lines. Results: The phenotypes of the most affected lines included severe stunting, leaf curling, darkened leaves characteristic of anthocyanin accumulation, delayed transition to flowering, low pollen and seed yields, and delayed senescence. Phenotype severity correlated with P450su1 transcript abundance, but not with transcript abundance of other experimental genes, strongly implicating CYP105A1 as responsible for the defects. Germination and seedling growth of transgenic and control lines in the presence and absence of 24-epibrassinolide indicated that CYP105A1 disrupts brassinosteroid signaling, most likely by inactivating brassinosteroids. Conclusions: Despite prior use of this gene as a genetic tool, deleterious growth in the absence of R7402 has not been elaborated. The authors show that this gene can cause aberrant growth by disrupting brassinosteroid signaling and affecting homeostasis.

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  • BMC Plant Biology, 11(67), BioMed Central, April 15, 2011, pp. 1-13

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  • Publication Title: BMC Plant Biology
  • Volume: 11
  • Issue: 67
  • Peer Reviewed: Yes

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  • April 15, 2011

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  • April 6, 2012, 2:30 p.m.

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  • Nov. 20, 2023, 10:45 a.m.

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Dasgupta, Kasturi; Ganesan, Savita; Manivasagam, Sindhu & Ayre, Brian G. A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling, article, April 15, 2011; [London, United Kingdom]. (https://digital.library.unt.edu/ark:/67531/metadc81383/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.

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